• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Epidermal stem cell dynamics during pregnancy

Research Project

Project/Area Number 19H03681
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 53050:Dermatology-related
Research InstitutionKyoto University

Principal Investigator

Toyoshima Fumiko  京都大学, ウイルス・再生医科学研究所, 教授 (40397576)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2019: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Keywords幹細胞 / 皮膚 / 妊娠 / 表皮幹細胞 / 血管 / 組織幹細胞 / 組織リモデリング
Outline of Research at the Start

表皮幹細胞の増殖・分化制御は、生体の恒常性維持に重要である。皮膚は、ライフステージごとの体型変化に伴い表面積を変化させるが、それを担う表皮幹細胞の制御機構や生理機能は不明である。申請者は、胎児の成長に伴って拡張する妊娠マウスの腹部皮膚において、表皮幹細胞から高い増殖能を持つ細胞群が産生されることを見出した。本研究では、妊娠に適応するための表皮幹細胞の増殖・分化制御機構を明らかにする。また、妊娠に伴う「真皮」の再編機構を解析し、表皮と真皮の相関を解明する。

Outline of Final Research Achievements

The skin surface area changes during life course. During pregnancy, the abdominal skin expands rapidly to accommodate the fetus grows, but its biological mechanism remains unknown.We previously reported that epidermal stem cells generate high proliferative basal cell progeny (Tbx3 + basal cells) that promotes skin expansion during pregnancy. In this study, we showed that Tbx3 + basal cells are vasculature-dependent epidermal stem cell population, which appear during pregnancy with a concomitant angiogenesis and undergo differentiation after parturition upon vasculature involution.In addition, when tension load was applied to the skin, skin vasculature increased even in non-pregnancy, and Tbx3 + basal cells were induced concomitantly, demonstrating that vasculatures and tension are important for skin extension. rice field.

Academic Significance and Societal Importance of the Research Achievements

妊娠期の皮膚リモデリング機構において、皮膚拡張を担う表皮細胞増殖クラスターの形成機構を解明し、その分子基盤を示した。また、血管新生を阻害すると表皮細胞増殖クラスターの出現が抑制されることを示し、皮膚リモデリング機構における血管の重要性を明らかにした。さらに、張力負荷により血管新生と表皮増殖クラスターが誘導されることを見出し、妊娠と張力による皮膚リモデリングの共通性を明らかにした。皮膚の医療・美容領域においては、力刺激を用いたケアが広く普及している。本研究は、これらの力学セラピーに対する生物学的根拠を提示するものである。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • Research Products

    (10 results)

All 2022 2021 2020 2019

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results)

  • [Journal Article] Delineation of biliary epithelial cell dynamics in maternal liver during pregnancy.2022

    • Author(s)
      Kozuki S, Sakurai S, Suzuki A, Yamamoto T, Toyoshima F.
    • Journal Title

      Genes Cells

      Volume: 27 Issue: 3 Pages: 192-201

    • DOI

      10.1111/gtc.12918

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs2021

    • Author(s)
      Ichijo R.、Kabata M.、Kidoya H.、Muramatsu F.、Ishibashi R.、Abe K.、Tsutsui K.、Kubo H.、Iizuka Y.、Kitano S.、Miyachi H.、Kubota Y.、Fujiwara H.、Sada A.、Yamamoto T.、Toyoshima F.
    • Journal Title

      Science Advances

      Volume: 7 Issue: 7

    • DOI

      10.1126/sciadv.abd2575

    • NAID

      120007181742

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence2020

    • Author(s)
      Ishibashi Riki、Abe Kota、Ido Nanami、Kitano Satsuki、Miyachi Hitoshi、Toyoshima Fumiko
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 14120-14133

    • DOI

      10.1038/s41598-020-70804-6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Skin remodeling during physiological body shape changes. Japan-Singapore Skin Webinar Series2021

    • Author(s)
      Toyoshima, F
    • Organizer
      Japan-Singapore Skin Webinar Series
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs2021

    • Author(s)
      Ryo Ichijo
    • Organizer
      27st East-Asia Symposium
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Vascular-driven stem cell states coordinates tissue scaling in dynamic skin2021

    • Author(s)
      Toyoshima, F
    • Organizer
      第94回日本生化学大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 皮膚拡張時における増殖能の高い表皮幹細胞の出現には血管が重要である2021

    • Author(s)
      一條遼
    • Organizer
      第73回日本細胞生物学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 妊娠期における臓器スケーリング2019

    • Author(s)
      豊島文子
    • Organizer
      ライフコースと個体および臓器スケーリング
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Defining the highly proliferative IFE stem cell state in regional skin homeostasis and remodeling2019

    • Author(s)
      Ryo Ichijo, Fumiko Toyoshima
    • Organizer
      Gordon Research Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 皮膚表皮幹細胞の性質は体表領域により異なる2019

    • Author(s)
      一條遼、佐田亜衣子、山本拓也、豊島文子
    • Organizer
      第42回 日本分子生物学会年会
    • Related Report
      2019 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi