Single-cell metabolomic and proteomic analyses in cancer cells by a novel mass spectrometry-based analytical system

• Project/Area Number: 19K05167
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 27040:Biofunction and bioprocess engineering-related
• Research Institution: Kyushu University
• Principal Investigator: Izumi Yoshihiro 九州大学, 生体防御医学研究所, 准教授 (70622166)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 1細胞メタボローム解析 / 1細胞プロテオーム解析 / シングルセル / 1細胞解析 / がん細胞 / メタボローム解析 / 細胞周期

Outline of Research at the Start

従来のメタボロームおよびプロテオーム解析は，技術的限界から，細胞集団 (1～100万個の細胞) ごとの分析が主であり，「時間および空間で平均した細胞の応答」を観測してきた．真の代謝の機能を見極めるためには，ヘテロな細胞集合体ではなく，個々の細胞に着目してメタボローム解析およびプロテオーム解析を実施することが望ましいと考えられる．本研究課題では，「ライブセルイメージング」×「1細胞サンプリング技術」×「超高感マルチ分子フェノタイプ解析」を融合した1細胞レベルでの代謝機能を網羅的かつ定量的に数値化するための基盤技術を創生し，がん細胞の細胞周期と代謝の差異を明らかにすることを目指す．

Outline of Final Research Achievements

Heterogeneous cell population including rare cancer cells (e.g., circulating tumor and stem cells) in blood and tissues is related to cancer development and drug resistance. Thus, information garnered from the analysis of samples extracted from a rare cancer single-cell is expected to advance knowledge of the mechanism of cancer development and the discovery of therapeutic strategy. However, mass spectrometry-based conventional proteomic and metabolomic analyses require large amount of starting materials (e.g., 10^5-10^6 cells) due to technical limitations. The aim of this study is to develop a novel analytical system for single-cell proteomics and metabolomics in mammalian cells by a combination of single-cell sampling, in-line sample preparation techniques, and highly sensitive nanoflow liquid chromatography tandem mass spectrometry (nano-LC/MS/MS).

Academic Significance and Societal Importance of the Research Achievements

本研究で開発した1細胞分子フェノタイプ解析システムは，申請者らの独自の要素技術が集約されたものであり，技術レベルはきわめて高い．今後，各種疾患関連試料の1細胞分子フェノタイプ解析を推進していくことで，従来の平均的観測では見出すことのできなかった重要なタンパク質や代謝物を発見し，それらの知見を基に疾患の機序解明あるいは鋭敏なバイオマーカー候補の探索に応用できる可能性がある．将来的には，当該技術はライフサイエンスの幅広い分野において貢献できると考えられる．

Research Products

• [Int'l Joint Research] Vrije Universiteit Brussel(ベルギー)
• [Journal Article] Quantitative metabolomics for dynamic metabolic engineering using stable isotope labeled internal standards mixture (SILIS). (2022)
  • Author(s): Soma Y#, Takahashi M#, Fujiwara Y, Tomiyasu N, Goto M, Hanai T, Izumi Y, Bamba T*.
  • Journal Title: J. Biosci. Bioeng. Volume: 133(1) Issue: 1 Pages: 46-55
  • DOI: 10.1016/j.jbiosc.2021.09.009
  • NAID: 40022801277
  • Peer Reviewed / Open Access
• [Journal Article] Comparative evaluation of plasma metabolomic data from multiple laboratories. (2022)
  • Author(s): Nishiumi S#*, Izumi Y#*, Hirayama A#*, Takahashi M, Nakao M, Hata K, Saigusa D, Hishinuma E, Matsukawa N, M Tokuoka S, Kita Y, Hamano F, Okahashi N, Ikeda K, Nakanishi H, Saito K, Yokota Hirai M, Yoshida M, Oda Y, Matsuda F, Bamba T.
  • Journal Title: Metabolites Volume: 12(2) Issue: 2 Pages: 135-135
  • DOI: 10.3390/metabo12020135
  • Peer Reviewed / Open Access
• [Journal Article] Targeting leukemia-specific dependence on the de novo purine synthesis pathway. (2022)
  • Author(s): Yamauchi T, Miyawaki K, Semba Y, Takahashi M, Izumi Y, Nogami J, Nakao F, Sugio T, Sasaki K, Pinello L, Bauer DE, Bamba T, Akashi K, Maeda T*.
  • Journal Title: Leukemia Volume: 36(2) Issue: 2 Pages: 383-393
  • DOI: 10.1038/s41375-021-01369-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Investigation of supercritical fluid chromatography retention behaviors using quantitative structure-retention relationships. (2022)
  • Author(s): Si-Hung L, Izumi Y, Nakao M, Takahashi M, Bamba T*.
  • Journal Title: Anal. Chim. Acta Volume: 1197 Pages: 339463-339463
  • DOI: 10.1016/j.aca.2022.339463
  • Peer Reviewed
• [Journal Article] Differential effect of canagliflozin, a sodium-glucose cotransporter (SGLT2) inhibitor, on slow and fast skeletal muscles from nondiabetic mice. (2022)
  • Author(s): Otsuka H, Yokomizo H*, Nakamura S, Izumi Y, Takahashi M, Obara S, Nakao M, Ikeda Y, Sato N, Sakamoto R, Miyachi Y, Miyazawa T, Bamba T, Ogawa Y*.
  • Journal Title: Biochem. J. Volume: 479(3) Issue: 3 Pages: 425-444
  • DOI: 10.1042/bcj20210700
  • Peer Reviewed / Open Access
• [Journal Article] Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis. (2022)
  • Author(s): Mise S#, Matsumoto A#*, Shimada K, Hosaka T, Takahashi M, Ichihara K, Shimizu H, Shiraishi C, Saito D, Suyama M, Yasuda T, Ide T, Izumi Y, Bamba T, Kimura-Someya T, Shirouzu M, Miyata H, Ikawa M, Nakayama KI*.
  • Journal Title: Nat. Commun. Volume: 13(1) Issue: 1 Pages: 1071-1071
  • DOI: 10.1038/s41467-022-28677-y
  • Peer Reviewed / Open Access
• [Journal Article] 次世代のメタボローム計測 (2021)
  • Author(s): 和泉自泰*
  • Journal Title: Seibutsu-kogaku Kaishi Volume: 99 Issue: 5 Pages: 229-232
  • DOI: 10.34565/seibutsukogaku.99.5_229
  • NAID: 130008042865
  • ISSN: 0919-3758, 2435-8630
  • Year and Date: 2021-05-25
• [Journal Article] Skeletal muscle-specific Keap1 disruption modulates fatty acid utilization and enhances exercise capacity in female mice. (2021)
  • Author(s): Onoki T, Izumi Y, Takahashi M, Murakami S, Matsumaru D, Ohta N, Wati SM, Hatanaka N, Katsuoka F, Okutsu M, Yabe Y, Hagiwara Y, Kanzaki M, Bamba T, Itoi E, Motohashi H*.
  • Journal Title: Redox Biol. Volume: 43 Pages: 101966-101966
  • DOI: 10.1016/j.redox.2021.101966
  • Peer Reviewed / Open Access
• [Journal Article] Performance of functionalized monolithic silica capillary columns with different mesopore sizes using radical polymerization of octadecyl methacrylate. (2021)
  • Author(s): Hara T, Baron G V, Hata K, Izumi Y, Bamba T*, Desmet G*
  • Journal Title: J. Chromatogr. A Volume: 1651 Pages: 462282-462282
  • DOI: 10.1016/j.chroma.2021.462282
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Design of synthetic quorum sensing achieving induction timing-independent signal stabilization for dynamic metabolic engineering of E. coli. (2021)
  • Author(s): Soma Y, Takahashi M, Fujiwara Y, Shinohara T, Izumi Y, Hanai T*, Bamba T*.
  • Journal Title: ACS Synth. Biol. Volume: 10(6) Issue: 6 Pages: 1384-1393
  • DOI: 10.1021/acssynbio.1c00008
  • Peer Reviewed
• [Journal Article] Produced β-hydroxybutyrate after β-hydroxy-β-methylbutyrate (HMB) administration may contribute HMB function in mice. (2021)
  • Author(s): Ikeda K#, Takahashi M#, Aburaya S, Harada D, Ikeda M, Kitagawa Y, Soma Y, Izumi Y, Bamba T, Furuse M*.
  • Journal Title: Biochem. Biophys. Rep. Volume: 27 Pages: 101097-101097
  • DOI: 10.1016/j.bbrep.2021.101097
  • Peer Reviewed / Open Access
• [Journal Article] Porphyromonas gingivalis induces entero-hepatic metabolic derangements with alteration of gut microbiota in a type 2 diabetes mouse model. (2021)
  • Author(s): Kashiwagi Y#, Aburaya S#, Sugiyama N#, Narukawa Y, Sakamoto Y, Takahashi M, Uemura H, Yamashita R, Tominaga S, Hayashi S, Nozaki T, Yamada S, Izumi Y, Kashiwagi A, Bamba T, Ishihama Y, Murakami S*.
  • Journal Title: Sci. Rep. Volume: 11(1) Issue: 1 Pages: 18398-18398
  • DOI: 10.1038/s41598-021-97868-2
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of kit-based metabolomics with other methodologies in a large cohort, towards establishing reference values. (2021)
  • Author(s): Saigusa D*, Hishinuma E, Matsukawa N, Takahashi M, Inoue J, Tadaka S, Motoike I N, Hozawa A, Izumi Y, Bamba T, Kinoshita K, Ekroos K, Koshiba S, Yamamoto M.
  • Journal Title: Metabolites Volume: 11(10) Issue: 10 Pages: 652-652
  • DOI: 10.3390/metabo11100652
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structural library and visualization of endogenously oxidized phosphatidylcholines using mass spectrometry-based techniques. (2021)
  • Author(s): Matsuoka Y, Takahashi M, Sugiura Y, Izumi Y, Nishiyama K, Nishida M, Suematsu M, Bamba T, Yamada KI*.
  • Journal Title: Nat. Commun. Volume: 12(1) Issue: 1 Pages: 6339-6339
  • DOI: 10.1038/s41467-021-26633-w
  • Peer Reviewed / Open Access
• [Journal Article] Detection and structural analysis of pyrimidine-derived radicals generated on DNA using a profluorescent nitroxide probe. (2021)
  • Author(s): Yamauchi K, Matsuoka Y, Takahashi M, Izumi Y, Taniguchi Y, Kawai K, Bamba T, Yamada KI*.
  • Journal Title: Chem. Commun. Volume: 58(1) Issue: 1 Pages: 56-59
  • DOI: 10.1039/d1cc04998d
  • Peer Reviewed / Open Access
• [Journal Article] Enzyme systems involved in glucosinolate metabolism in Companilactobacillus farciminis KB1089. (2021)
  • Author(s): Watanabe H#, Usami R#, Kishino S#, Osada K, Aoki Y, Morisaka H, Takahashi M, Izumi Y, Bamba T, Aoki W, Suganuma H, Ogawa J*.
  • Journal Title: Sci. Rep. Volume: 11(1) Issue: 1 Pages: 23715-23715
  • DOI: 10.1038/s41598-021-03064-7
  • Peer Reviewed / Open Access
• [Journal Article] Helicobacter metabolites exacerbate gastritis through C-type lectin receptors. (2021)
  • Author(s): Nagata M, Toyonaga K, Ishikawa E, Haji S, Okahashi N, Takahashi M, Izumi Y, Imamura A, Takato K, Ishida H, Nagai S, Illarionov P, Stocker B L, Timmer M S M, Smith D G M, Williams S J, Bamba T, Miyamoto T, Arita M, Appelmelk B J, Yamasaki S*.
  • Journal Title: J. Exp. Med. Volume: 218(1) Issue: 1
  • DOI: 10.1084/jem.20200815
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Glioma cells require one-carbon metabolism to survive glutamine starvation. (2021)
  • Author(s): Tanaka K*, Sasayama T, Nagashima H, Irino Y, Takahashi M, Izumi Y, Uno T, Satoh N, Kitta A, Kyotani K, Fujita Y, Hashiguchi M, Nakai T, Kohta M, Uozumi Y, Shinohara M, Hosoda K, Bamba T, Kohmura E.
  • Journal Title: Acta Neuropathol. Commun. Volume: 9 Issue: 1 Pages: 16-16
  • DOI: 10.1186/s40478-020-01114-1
  • NAID: 120006956370
  • Peer Reviewed / Open Access
• [Journal Article] Ddhd1 knockout mouse as a model of locomotive and physiological abnormality in familial spastic paraplegia. (2021)
  • Author(s): Morikawa T, Ohishi H, Kosaka K, Shimojo T, Nagano A, Taniguchi I, Fujioka R, Moriyama K, Unoki M, Takahashi M, Nakao M, Izumi Y, Bamba T, Sasaki H, Miura S, Shibata H*.
  • Journal Title: Biosci. Rep. Volume: 41(2) Issue: 2
  • DOI: 10.1042/bsr20204171
  • Peer Reviewed
• [Journal Article] Metabolic alteration of Methylococcus capsulatus str. Bath during a microbial gas-phase reaction. (2021)
  • Author(s): Chen Y-Y#, Soma Y#, Ishikawa M#, Takahashi M, Izumi Y, Bamba T, Hori K*.
  • Journal Title: Bioresour. Technol. Volume: 330 Pages: 125002-125002
  • DOI: 10.1016/j.biortech.2021.125002
  • Peer Reviewed
• [Journal Article] Calibration-curve-locking database for semi-quantitative metabolomics by gas chromatography/mass spectrometry. (2021)
  • Author(s): Hata K#, Soma Y#, Yamashita T, Takahashi M, Sugitate K, Serino T, Miyagawa H, Suzuki K, Yamada K, Kawamukai T, Shiota T, Izumi Y*, Bamba T*.
  • Journal Title: Metabolites Volume: 11(4) Issue: 4 Pages: 207-207
  • DOI: 10.3390/metabo11040207
  • Peer Reviewed / Open Access
• [Journal Article] 1細胞メタボローム解析の現状 (2021)
  • Author(s): 和泉自泰*，馬場 健史．
  • Journal Title: 医学のあゆみ Volume: 276(10) Pages: 927-932
• [Journal Article] Nano-Liquid Chromatography Mass Spectrometry-Based Molecular and Phenotypic Analysis at Single-Cell Resolution (2020)
  • Author(s): 1.和泉自泰*，中谷航太，秦康祐，原健士，松本雅記，馬場健史．
  • Journal Title: Journal of the Mass Spectrometry Society of Japan Volume: 68 Issue: 2 Pages: 44-48
  • DOI: 10.5702/massspec.S20-09
  • NAID: 130007831565
  • ISSN: 1340-8097, 1880-4225, 1884-3271
  • Year and Date: 2020-04-01
  • Peer Reviewed
• [Journal Article] An Analytical System for Single-Cell Metabolomics of Typical Mammalian Cells Based on Highly Sensitive Nano-Liquid Chromatography Tandem Mass Spectrometry (2020)
  • Author(s): Nakatani K, Izumi Y*, Hata K, Bamba T.
  • Journal Title: Mass Spectrometry Volume: 9 Issue: 1 Pages: A0080-A0080
  • DOI: 10.5702/massspectrometry.A0080
  • NAID: 130007809373
  • ISSN: 2186-5116, 2187-137X
  • Year and Date: 2020-03-17
  • Peer Reviewed / Open Access
• [Journal Article] Method for Structural Determination of Lipid-Derived Radicals (2020)
  • Author(s): Matsuoka Yuta、Izumi Yoshihiro、Takahashi Masatomo、Bamba Takeshi、Yamada Ken-ichi
  • Journal Title: Analytical Chemistry Volume: - Issue: 10 Pages: 6993-7002
  • DOI: 10.1021/acs.analchem.0c00053
  • Peer Reviewed
• [Journal Article] Eicosapentaenoic acid attenuates renal lipotoxicity by restoring autophagic flux. (2020)
  • Author(s): Yamamoto T, Takabatake Y*, Minami S, Takahashi A, Sakai S, Fujimura R, Takahashi A, Namba-Hamano T, Matsuda J, Kimura T, Matsui I, Kaimori J, Takeda H, Takahashi M, Izumi Y, Bamba T, Matsusaka T, Niimura F, Yanagita M, Isaka Y.
  • Journal Title: Autophagy Volume: 28 Issue: 7 Pages: 1-14
  • DOI: 10.1080/15548627.2020.1782034
  • Peer Reviewed
• [Journal Article] Development of a novel method for polar metabolite profiling by supercritical fluid chromatography/tandem mass spectrometry. (2020)
  • Author(s): Konya Y, Izumi Y, Bamba T*.
  • Journal Title: J. Chromatogr. A Volume: 1632 Pages: 461587-461587
  • DOI: 10.1016/j.chroma.2020.461587
  • Peer Reviewed
• [Journal Article] Dynamic metabolome analysis reveals the metabolic fate of medium-chain fatty acid in AML12 cells. (2020)
  • Author(s): Fushimi T, Izumi Y*, Takahashi M, Hata K, Murano Y, Bamba T.
  • Journal Title: J. Agric. Food Chem. Volume: 68(43) Issue: 43 Pages: 11997-12010
  • DOI: 10.1021/acs.jafc.0c04723
  • Peer Reviewed
• [Journal Article] 超臨界流体クロマトグラフィー質量分析を基盤とした定量リピドーム分析法の開発． (2020)
  • Author(s): 和泉自泰*，馬場健史．
  • Journal Title: 生物工学 Volume: 98(9) Pages: 490-493
• [Journal Article] ω-3 脂肪酸含有脂質を識別する定量リピドーム解析技術． (2020)
  • Author(s): 竹田浩章，和泉自泰，馬場 健史*．
  • Journal Title: バイオインダストリー Volume: 37(10) Pages: 32-40
• [Journal Article] アルコール代謝研究の最新動向． (2020)
  • Author(s): 和泉自泰*．
  • Journal Title: 生物工学 Volume: 98(11) Pages: 622-622
• [Journal Article] Performance of small-domain monolithic silica columns in nano-liquid chromatography and comparison with commercial packed bed columns with 2 μm particles. (2020)
  • Author(s): Hara T, Izumi Y, Hata K, Baron G V, Bamba T, Desmet G*
  • Journal Title: J. Chromatogr. A Volume: 1616 Pages: 460804-460804
  • DOI: 10.1016/j.chroma.2019.460804
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Metabolic effects of RUBCN/Rubicon deficiency in kidney proximal tubular epithelial cells. (2020)
  • Author(s): Matsuda J, Takahashi A, Takabatake Y*, Sakai S, Minami S, Yamamoto T, Fujimura R, Namba-Hamano T, Yonishi H, Nakamura J, Kimura T, Kaimori J, Matsui I, Takahashi M, Nakao M, Izumi Y, Bamba T, Matsusaka T, Niimura F, Yanagita M, Yoshimori T, Isaka Y.
  • Journal Title: Autophagy Volume: 16 Issue: 10 Pages: 1-16
  • DOI: 10.1080/15548627.2020.1712107
  • Peer Reviewed
• [Journal Article] In-line sample processing system with an immobilized trypsin-packed fused-silica capillary tube for proteomic analysis of a small number of mammalian cells. (2020)
  • Author(s): Hata K, Izumi Y*, Hara T, Matsumoto M*, Bamba T.
  • Journal Title: Anal. Chem. Volume: 92(4) Issue: 4 Pages: 2997-3005
  • DOI: 10.1021/acs.analchem.9b03993
  • Peer Reviewed
• [Journal Article] Lipid profiling of serum and lipoprotein fractions in response to pitavastatin using an animal model of familial hypercholesterolemia. (2020)
  • Author(s): Takeda H, Izumi Y*, Tamura S, Koike T, Koike Y, Shiomi M, Bamba T.
  • Journal Title: J. Proteome Res. Volume: 19(3) Issue: 3 Pages: 1100-1108
  • DOI: 10.1021/acs.jproteome.9b00602
  • Peer Reviewed
• [Journal Article] A shift in glutamine nitrogen metabolism contributes to malignant progression of cancer. (2020)
  • Author(s): Kodama M, Oshikawa K, Shimizu H, Yoshioka S, Takahashi M, Izumi Y, Bamba T, Tateishi C, Tomonaga T, Matsumoto M*, Nakayama KI*.
  • Journal Title: Nat. Commun. Volume: 11 Issue: 1 Pages: 1320-1320
  • DOI: 10.1038/s41467-020-15136-9
  • Peer Reviewed / Open Access
• [Journal Article] Lipidomic analysis of cells and extracellular vesicles from high- and low-metastatic triple-negative breast cancer. (2020)
  • Author(s): Nishida-Aoki N, Izumi Y*, Takeda H, Takahashi M, Ochiya T*, Bamba T.
  • Journal Title: Metabolites Volume: 10(2) Issue: 2 Pages: 67-67
  • DOI: 10.3390/metabo10020067
  • Peer Reviewed / Open Access
• [Journal Article] Identification of acrylamide adducts generated during storage of canned milk coffee. (2020)
  • Author(s): Yoshioka T, Izumi Y*, Takahashi M, Suzuki K, Miyamoto Y, Nagatomi Y, Bamba T*
  • Journal Title: J. Agric. Food Chem. Volume: 68 Issue: 12 Pages: 3859-3867
  • DOI: 10.1021/acs.jafc.9b08139
  • Peer Reviewed
• [Journal Article] A highly sensitive determination method for acrylamide in beverages, grains, and confectioneries by supercritical fluid chromatography tandem mass spectrometry. (2019)
  • Author(s): Yoshioka T, Izumi Y, Nagatomi Y, Miyamoto Y, Suzuki K, Bamba T*.
  • Journal Title: Food Chem. Volume: 294 Pages: 486-492
  • DOI: 10.1016/j.foodchem.2019.05.033
  • Peer Reviewed
• [Journal Article] Improved quantitation of lipid classes using supercritical fluid chromatography with charged aerosol detector. (2019)
  • Author(s): Takeda H, Takahashi M, Hara T, Izumi Y, Bamba T*
  • Journal Title: J. Lipid Res. Volume: 60(8) Issue: 8 Pages: 1465-1474
  • DOI: 10.1194/jlr.d094516
  • Peer Reviewed
• [Journal Article] Comparison of retention behavior between supercritical fluid chromatography and normal-phase high performance liquid chromatography with various stationary phases. (2019)
  • Author(s): Hirose T*, Keck D, Izumi Y, Bamba T.
  • Journal Title: Molecules Volume: 24(13) Issue: 13 Pages: 2425-2425
  • DOI: 10.3390/molecules24132425
  • Peer Reviewed / Open Access
• [Journal Article] A pilot study: Effects of Kombu intake on lifestyle-related diseases, Possibility that an intake of Kombu is effective for the subjects with abnormally high serum triglyceride levels. (2019)
  • Author(s): Nishiumi S*, Izumi Y, Kobayashi T, Yoshida M*
  • Journal Title: Food Sci. Technol. Res. Volume: 25(6) Issue: 6 Pages: 827-834
  • DOI: 10.3136/fstr.25.827
  • NAID: 130007771301
  • Peer Reviewed
• [Journal Article] Inter-Laboratory Comparison of Metabolite Measurements for Metabolomics Data Integration (2019)
  • Author(s): Izumi Yoshihiro、Matsuda Fumio、Hirayama Akiyoshi、Ikeda Kazutaka、Kita Yoshihiro、Horie Kanta、Saigusa Daisuke、Saito Kosuke、Sawada Yuji、Nakanishi Hiroki、Okahashi Nobuyuki、Takahashi Masatomo、Nakao Motonao、Hata Kosuke、Hoshi Yutaro、Morihara Motohiko、Tanabe Kazuhiro、Bamba Takeshi、Oda Yoshiya
  • Journal Title: Metabolites Volume: 9 Issue: 11 Pages: 257-257
  • DOI: 10.3390/metabo9110257
  • Peer Reviewed / Open Access
• [Presentation] 高感度nano-uHILIC/AEX/MS/MSによる少数細胞集団試料のメタボローム分析 (2021)
  • Author(s): 〇中谷 航太，和泉 自泰，秦 康祐，高橋 政友，内田 穣，石井 優，馬場 健史
  • Organizer: 第69回 質量分析総合討論会
• [Presentation] メタボロミクスデータ統合に向けた施設間比較の検証と今後の課題 (2021)
  • Author(s): 〇和泉 自泰
  • Organizer: 第69回 質量分析総合討論会
• [Presentation] LC/MSを基盤とした血漿メタボローム分析法の性能評価 (2021)
  • Author(s): 〇今戸 優理，油屋 駿介，高橋 政友，中谷 航太，相馬 悠希，和泉 自泰，馬場 健史
  • Organizer: 第69回 質量分析総合討論会
• [Presentation] 免疫受容体の新規脂質リガンド探索のためのLC-TriVersa NanoMate/HRMS/MSを基盤とした解析プラットフォームの開発 (2021)
  • Author(s): 〇富安 範行，和泉 自泰，高橋 政友，西村 直矢，豊永 憲司，相馬 悠希，山崎 晶，馬場 健史
  • Organizer: 第69回 質量分析総合討論会
• [Presentation] ナノ液体クロマトグラフィー質量分析を基盤としたシングルセル分子フェノタイプ解析 (2021)
  • Author(s): 〇和泉 自泰，馬場 健史
  • Organizer: 代謝統合オミクス 第2回 新学術計測セミナー
  • Invited
• [Presentation] Development of a widely-targeted quantitative lipidomics methodology by supercritical fluid chromatography coupled with triple quadrupole mass spectrometry (2021)
  • Author(s): Hiroaki Takeda, Yoshihiro Izumi, Masatomo Takahashi, Motonao Nakao, 〇Takeshi Bamba
  • Organizer: Metabolomics 2021
  • Int'l Joint Research
• [Presentation] Remote solid cancers rewire hepatic nitrogen metabolism via host nicotinamide-N-methyltransferase (2021)
  • Author(s): Rin Mizuno, Hiroaki Hojo, Masatomo Takahashi, Soshiro Kashio, Sora Enya, Motonao Nakao, Riyo Konishi, Junzo Hamanishi, Hiroshi Kawamoto, Masaki Mandai, Yutaka Suzuki, Masayuki Miura, Takeshi Bamba, Yoshihiro Izumi, 〇Shinpei Kawaoka
  • Organizer: 6th Cancer Cachexia Conference
  • Int'l Joint Research
• [Presentation] 質量分析を基盤とした神経幹細胞のマルチオミクス解析 (2021)
  • Author(s): 〇和泉 自泰
  • Organizer: 第64回 日本神経化学会大会
  • Invited
• [Presentation] シングルセル最先端解析技術の紹介 (2021)
  • Author(s): 〇竹山 春子，〇松永 浩子，〇秦 康祐，〇中谷 航太，〇和泉 自泰，馬場 健史
  • Organizer: 早稲田大学 BINDS ユニット連携講習会
• [Presentation] 定量リピドーム分析によるエクソソーム解析 (2021)
  • Author(s): 〇和泉 自泰
  • Organizer: 第15回 メタボロームシンポジウム
  • Invited
• [Presentation] Vitamin B6 is an Oxygen-Sensitive Nutrient Shaping Macrophage Inflammatory Phenotype under Chronic Hypoxia by Suppressing Lysosomal Activity (2021)
  • Author(s): 〇Hiroki Sekine, Akihiro Kishino, Hayato Anzawa, Yoshihiro Izumi, Masatomo Takahashi, Takeshi Bamba, Kengo Kinoshita, Hozumi Motohashi
  • Organizer: Cold Spring Harbor Laboratory meeting
  • Int'l Joint Research
• [Presentation] ナノ液体クロマトグラフィー質量分析を基盤としたシングルセル分子フェノタイプ解析法の開発 (2021)
  • Author(s): 〇和泉 自泰，秦 康祐，中谷 航太，山村 昌平，松本 雅記，馬場 健史
  • Organizer: 第94回 日本生化学会大会
  • Invited
• [Presentation] Newly developed mass spectrometry-based analytical system for single-cell metabolomics and proteomics (2021)
  • Author(s): 〇Yoshihiro Izumi, Kosuke Hata, Kohta Nakatani, Shohei Yamamura, Masaki Matsumoto, Takeshi Bamba
  • Organizer: The 26th Symposium of Young Asian Biological Engineers’ Community
  • Int'l Joint Research
• [Presentation] Challenge to single-cell metabolome and proteome analyses (2021)
  • Author(s): 〇Yoshihiro Izumi, Kohta Nakatani, Kosuke Hata, Shohei Yamamura, Masaki Matsumoto, Takeshi Bamba
  • Organizer: 第59回 日本生物物理学会年会
  • Invited
• [Presentation] 呼吸鎖阻害に対するがん細胞と出芽酵母の代謝アダプテーション解析 (2021)
  • Author(s): 〇澤井 茜，清家 泰介，岡橋 伸幸，和泉 自泰，馬場 健史，松田 史生
  • Organizer: 第44回 日本分子生物学会年会
• [Presentation] 持続的NFκBの持続的な活性化による細胞老化の誘導 (2021)
  • Author(s): 〇田畑 祥，溝口 亜紀美，Miguel Luis A. Francisco，高橋 政友，和泉 自泰，馬場 健史，岡田 眞里子
  • Organizer: 第44回 日本分子生物学会年会
• [Presentation] LC-nESI-HRMS/MSを基盤とした免疫受容体の脂質リガンド探索プラットフォームの開発 (2021)
  • Author(s): ◯富安 範行，和泉 自泰，高橋 政友，西村 直矢，豊永 憲司，相馬 悠希，山崎 晶，馬場 健史
  • Organizer: 第27回 日本生物工学会九州支部 大分大会（2021）
• [Presentation] エクソソームの定量リピドーム解析 (2021)
  • Author(s): 〇和泉 自泰, 西田 奈央, 竹田 浩章, 高橋 政友, 落谷 孝広, 馬場 健史
  • Organizer: 第3回 脂質駆動学術産業創生研究部会講演会
  • Invited
• [Presentation] メタボロミクスを活用した肝細胞における中鎖脂肪酸代謝物解析 (2021)
  • Author(s): 〇伏見 達也，和泉 自泰，高橋 政友，秦 康祐，村野 賢博，馬場 健史
  • Organizer: 第3回 脂質駆動学術産業創生研究部会講演会
• [Presentation] 親水性代謝物のワイドターゲットメタボローム分析のプロトコル (2021)
  • Author(s): ○和泉 自泰
  • Organizer: 日本農芸化学会 2021年度大会
  • Invited
• [Presentation] 高感度ナノ液体クロマトグラフィータンデム質量分析を基盤とした動物細胞のシングルセルメタボローム分析 (2020)
  • Author(s): ○中谷 航太，和泉 自泰，秦 康祐，馬場 健史
  • Organizer: 第68回質量分析総合討論会
• [Presentation] ノンターゲットメタボロミクスに向けた基盤技術開発 (2020)
  • Author(s): ○和泉 自泰，鳥越 大平，富安 範行，中谷 航太，高橋 政友，馬場 健史
  • Organizer: 第68回質量分析総合討論会
• [Presentation] イオンクロマトグラフィー四重極オービトラップ型高分解能質量分析 (IC/HRMS/MS) を用いた次世代メタボローム分析法の開発 (2020)
  • Author(s): ○高橋 政友1，和泉 自泰1，鈴木 隆弘2，中谷 航太1，秦 康祐1，高原 健太郎2，馬場 健史1
  • Organizer: 第68回質量分析総合討論会
• [Presentation] Exploratory approaches to highly-sensitive analysis toward single-cell shotgun proteomics using nano-LC/MS/MS (2020)
  • Author(s): ○Kosuke Hata1, Takeshi Hara1, Yoshihiro Izumi1, Masaki Matsumoto1,2, Takeshi Bamba1
  • Organizer: 68th ASMS Conference on Mass Spectrometry and Allied Topics
  • Int'l Joint Research
• [Presentation] Single-cell metabolomics of hydrophilic metabolites in typical mammalian cells based on highly sensitive nano-liquid chromatography tandem mass spectrometry (2020)
  • Author(s): ○Kohta Nakatani, Yoshihiro Izumi, Kosuke Hata, Takeshi Bamba
  • Organizer: 68th ASMS Conference on Mass Spectrometry and Allied Topics
  • Int'l Joint Research
• [Presentation] 次世代のメタボローム計測 (2020)
  • Author(s): ○和泉 自泰
  • Organizer: 生物工学会バイオ計測サイエンス研究部会2020 シンポジウム
  • Invited
• [Presentation] ナノ液体クロマトグラフィー質量分析を基盤としたシングルセル分子フェノタイプ解析 (2020)
  • Author(s): ○和泉 自泰
  • Organizer: CBI Annual Meeting 2020
  • Invited
• [Presentation] 免疫受容体に対する新規脂質リガンド探索のための解析プラットフォームの開発 (2020)
  • Author(s): ○富安 範行1，和泉 自泰1,2，髙橋 政友2，西村 直矢3，豊永 憲司3，山崎 晶3，馬場 健史1,2
  • Organizer: 生物工学若手研究者の集い 第三回 オンラインセミナー
• [Presentation] Single-run comprehensive hydrophilic metabolome analysis by unified hydrophilic-interaction/anion-exchange liquid chromatography mass spectrometry (2020)
  • Author(s): Kohta Nakatani1, Yoshihiro Izumi1, Masatomo Takahashi1, Keita Sakurai, Michio Butsugan, Takeshi Bamba1
  • Organizer: AOMSC 2020
  • Int'l Joint Research
• [Presentation] Inter-laboratory comparison of metabolite measurements for metabolomics data integration (2020)
  • Author(s): Yoshihiro Izumi
  • Organizer: The 29th Hot Spring Harbor International Symposium
  • Int'l Joint Research / Invited
• [Presentation] 親水性相互作用/陰イオン交換クロマトグラフィータンデム質量分析による次世代メタボローム分析法の開発 (2019)
  • Author(s): 中谷 航太1，○和泉 自泰1，高橋 政友1，櫻井 恵太2，佛願 道男2，馬場 健史1
  • Organizer: 第67回 質量分析総合討論会
• [Presentation] A Novel Mass Spectrometry-Based Analytical System for Single-Cell Proteomics and Metabolomics in Mammalian Cells (2019)
  • Author(s): Yoshihiro Izumi1, Kousuke Hata1, Kohta Nakatani1, Takeshi Hara1, Shohei Yamamura2, Masaki Matsumoto1, Takeshi Bamba1
  • Organizer: 67th ASMS Conference on Mass Spectrometry and Allied Topics
  • Int'l Joint Research
• [Presentation] Large-Scale Metabolomic Analysis of Hydrophilic Metabolites Using Hydrophilic Interaction Liquid Chromatography Tandem Mass Spectrometry with a Novel Polymer-Based Amino Column (2019)
  • Author(s): Kohta Nakatani1, Yoshihiro Izumi1, Masatomo Takahashi1, Keita Sakurai2, Michio Butsugan2, Takeshi Bamba1
  • Organizer: 67th ASMS Conference on Mass Spectrometry and Allied Topics
  • Int'l Joint Research
• [Presentation] Novel analytical method for global metabolomic analysis by hydrophilic interaction/anion-exchange liquid chromatography tandem mass spectrometry (2019)
  • Author(s): Kohta Nakatani1, Yoshihiro Izumi1, Masatomo Takahashi1, Keita Sakurai2, Michio Butsugan2, Takeshi Bamba1
  • Organizer: 15th Annual Conference of the Metabolomics Society (Metabolomics 2019)
  • Int'l Joint Research
• [Presentation] シングルセル分子フェノタイプ解析に向けた基盤技術の創生 (2019)
  • Author(s): 和泉 自泰1，松本 雅記1，山村 昌平2，馬場 健史1
  • Organizer: 日本プロテオーム学会2019年大会 第70回日本電気泳動学会総会
  • Invited
• [Presentation] シングルセルプロテオミクスに資する細胞回収前処理装置の開発 (2019)
  • Author(s): 秦 康祐1，和泉 自泰1，原 健士1，平藤 衛2，山村 昌平3，松本 雅記1，馬場 健史1
  • Organizer: 日本プロテオーム学会2019年大会 第70回日本電気泳動学会総会
• [Presentation] 次世代メタボローム分析法：親水性相互作用/陰イオン交換クロマトグラフィータンデム質量分析の開発 (2019)
  • Author(s): 中谷 航太1，和泉 自泰1，高橋 政友1，櫻井 恵太2，佛願 道男2，○馬場 健史1
  • Organizer: 日本プロテオーム学会2019年大会 第70回日本電気泳動学会総会
• [Presentation] 1細胞マルチ分子フェノタイピング技術の開発 (2019)
  • Author(s): 馬場 健史，和泉 自泰
  • Organizer: 京都バイオ計測センター・京都大学ナノハブ拠点連携シンポジウム
  • Invited
• [Presentation] 次世代メタボロミクスに向けた技術開発 (2019)
  • Author(s): 和泉 自泰
  • Organizer: JASIS 2019
  • Invited
• [Presentation] 質量分析を基盤としたシングルセル分子フェノタイプ解析 (2019)
  • Author(s): 和泉 自泰，馬場 健史
  • Organizer: 第71回 日本生物工学会大会
  • Invited
• [Presentation] 親水性相互作用/陰イオン交換クロマトグラフィータンデム質量分析による親水性代謝物の一斉 (2019)
  • Author(s): 中谷 航太1，和泉 自泰1，高橋 政友1，櫻井 恵太2，佛願 道男2，馬場 健史1
  • Organizer: 第71回 日本生物工学会大会
• [Presentation] 1細胞分子フェノタイプ解析に向けた基盤技術の創生 (2019)
  • Author(s): 和泉 自泰，馬場 健史
  • Organizer: Single Cellome Co-Innovators’Consortium 第1回 オープンフォーラム
  • Invited
• [Presentation] Unified HILIC/AEX/MS/MSによる新規メタボローム分析法の開発 (2019)
  • Author(s): 和泉 自泰1，中谷 航太1，櫻井 恵太2，佛願 道男2，馬場 健史1
  • Organizer: 第13回 メタボロームシンポジウム
• [Presentation] Next-generation metabolomics: Technological development and medical application (2019)
  • Author(s): Yoshihiro Izumi
  • Organizer: The 4th Symposium of the Inter-University Research Network for Trans-Omics Medicine
  • Int'l Joint Research / Invited
• [Presentation] System Development of a method for single-cell shotgun proteomics using highly sensitive nano-LC/MS/MS (2019)
  • Author(s): Kousuke Hata1, Takeshi Hara1, Yoshihiro Izumi1, Masaki Matsumot2, 3, Takeshi Bamba1
  • Organizer: HPLC 2019 Kyoto
  • Int'l Joint Research
• [Presentation] Exploring column performance of monolithic silica capillary columns and its application in nano-LC (2019)
  • Author(s): Takeshi Hara1, Kosuke Hata1, Yoshihiro Izumi1, Gino.V. Baron2, Takeshi Bamba1 and Gert
  • Organizer: HPLC 2019 Kyoto
  • Int'l Joint Research
• [Presentation] Unified HILIC/AEX/MS/MSによる次世代メタボローム分析法の開発 (2019)
  • Author(s): 和泉 自泰1, 中谷 航太1, 高橋 政友1, 櫻井 恵太2, 佛願 道男2, 馬場 健史1
  • Organizer: 第30回 クロマトグラフィー科学会議
• [Patent(Industrial Property Rights)] 質量分析用標識組成物，代謝物の定量分析法，及び代謝物の動態解析法 (2019)
  • Inventor(s): 脇村明夏里，寺内勉，馬場健史，和泉自泰，相馬悠希
  • Industrial Property Rights Holder: 脇村明夏里，寺内勉，馬場健史，和泉自泰，相馬悠希
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2019-090121
  • Filing Date: 2019