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Development of nanobio device with carbon nanotube and enzyme from thermophile

Research Project

Project/Area Number 19K05172
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 27040:Biofunction and bioprocess engineering-related
Research InstitutionShibaura Institute of Technology

Principal Investigator

Muguruma Hitoshi  芝浦工業大学, 工学部, 教授 (20309719)

Co-Investigator(Kenkyū-buntansha) 平塚 淳典  国立研究開発法人産業技術総合研究所, 材料・化学領域, 研究グループ長 (70392652)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsカーボンナノチューブ / バイオセンサ / 酵素燃料電池 / 酵素 / 電気化学 / バイオデバイス / 単層カーボンナノチューブ / アノード / カソード / 燃料電池
Outline of Research at the Start

カーボンナノチューブと好熱菌酵素からなるナノバイオ融合デバイスの開発を行う。研究遂行には、応募者が開発した技術、(1)長寿命、高出力、高温動作が見込める好熱性菌由来の酵素技術、(2)酵素から電極へのエネルギーの取り込みとして、完全孤立した単層カーボンナノチューブによる直接電子移動機構、(3)実用化・産業応用のための簡便で量産性の高い作製方法開発のためのナノバイオ界面技術、を集約発展させる。

Outline of Final Research Achievements

As energy-creating technologies that contribute to the reduction of renewable energy and carbon dioxide, we will develop long-life, room temperature to high temperature operation, safety, and high output nanobiofusion enzyme fuel cells and self-powered biosensors. For that purpose, we utilized the basic technology already developed by the applicant, that is, an enzyme derived from thermophile that is stable for a long time, a method of dispersing single-walled carbon nanotubes in an aqueous solution, and a simple bio-nano interface preparation method. As a result, we obtained research results such as the development of high-performance single-walled carbon nanotubes and the development of enzyme fuel cells and enzyme biosensor prototypes.

Academic Significance and Societal Importance of the Research Achievements

スマートウォッチの登場でウェアラブル端末が身近となり、さらに先を行くものとして注目されているのが人体に直接埋め込んだり貼り付けたりするIoT・ウェアラブル端末である。現在までグーグルによるコンタクトレンズ型血糖センサ等が開発段階にあり、既に医療用体内埋め込み型インシュリンポンプ、血糖モニタリング等が実用化されている。生体埋込機器で課題となるのは、長期間連続使用可能な電源の確保およびその安全性である。このようなデバイスは数年~数10年以上にわたり交換なしでの電源供給が必要である。この要請に応えうる研究成果を得た。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (25 results)

All 2022 2021 2020 2019 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 4 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Direct Electron Transfer between Single-Walled Carbon Nanotube and Fructose Dehydrogenase2021

    • Author(s)
      Toya Fukuda, Hitoshi Muguruma, Hisanori Iwasa, Atsunori Hiratsuka, Takeshi Tanaka, Katsumi Tsuji, Takahide Kishimoto
    • Journal Title

      IEEE Transactions on Nanotechnology

      Volume: 20 Pages: 610-618

    • DOI

      10.1109/tnano.2021.3100817

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Detection of Curcumin in Food with a Carbon Nanotube-Carboxymethylcellulose Electrode2020

    • Author(s)
      R. Wada, S. Takahashi, H. Muguruma, N. Osakabe
    • Journal Title

      Analytical Sciences

      Volume: 36 Issue: 9 Pages: 1113-1118

    • DOI

      10.2116/analsci.20P021

    • NAID

      130007898782

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2020-09-10
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Xylose-Insensitive Direct Electron Transfer Biosensor Strip with Single-Walled Carbon Nanotubes and Novel Fungal Flavin Adenine Dinucleotide Glucose Dehydrogenase2020

    • Author(s)
      H. Iwasa, A. Hiratsuka, T. Tanaka, K. Tsuji, T. Kishimoto, Y. Watanabe, Y. Hoshino, H. Muguruma,
    • Journal Title

      IEEE Sensors Journal

      Volume: 20 Issue: 21 Pages: 12522-12529

    • DOI

      10.1109/jsen.2020.3001606

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] New perspective on ECE mechanism of monohydroxycinnamic acid oxidation with carbon nanotube electrode2020

    • Author(s)
      R. Wada, S. Takahashi, H. Muguruma,
    • Journal Title

      Electrochimica Acta

      Volume: 359 Pages: 136964-136964

    • DOI

      10.1016/j.electacta.2020.136964

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis Chlorogenic Acids in Coffee with A Multi-walled Carbon Nanotube Electrode2020

    • Author(s)
      S. Takahashi, R. Wada, H. Muguruma, N. Osakabe
    • Journal Title

      Food Analytical Methods

      Volume: 13 Issue: 4 Pages: 923-932

    • DOI

      10.1007/s12161-020-01714-6

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Electrochemical determination of uric acid in urine and serum with uricase/carbon nanotube /carboxymethylcellulose electrode2020

    • Author(s)
      T. Fukuda, H. Muguruma, H. Iwasa, T. Tanaka, A. Hiratsuka, T. Shimizu, K. Tsuji, T. Kishimoto
    • Journal Title

      Analytical Biochemistry

      Volume: 590 Pages: 113533-113533

    • DOI

      10.1016/j.ab.2019.113533

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Selective Detection of Rutin in The Presence of Ascorbic Acid with A Carbon Nanotube Electrode2020

    • Author(s)
      S. Takahashi, H. Muguruma, N. Osakabe, H. Inoue, T. Ohsawa
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 59 Issue: SD Pages: SDDD02-SDDD02

    • DOI

      10.7567/1347-4065/ab4ee4

    • NAID

      210000157391

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Simultaneous Electrochemical Determination of Isoquercitrin and Epigallocatechingallate at a Carbon Nanotube Electrode2019

    • Author(s)
      S. Takahashi, H. Muguruma, N. Osakabe, H. Inoue, T. Ohsawa
    • Journal Title

      Electrochemistry

      Volume: 87 Issue: 4 Pages: 242-244

    • DOI

      10.5796/electrochemistry.19-00020

    • NAID

      130007674916

    • ISSN
      1344-3542, 2186-2451
    • Year and Date
      2019-07-05
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Polyphenol Analysis in Black Tea with a Carbon Nanotube Electrode2019

    • Author(s)
      MURAKAMI Satoshi、TAKAHASHI Shota、MUGURUMA Hitoshi、OSAKABE Naomi、INOUE Hitoshi、OHSAWA Tatsuya
    • Journal Title

      Analytical Sciences

      Volume: 35 Issue: 5 Pages: 529-534

    • DOI

      10.2116/analsci.18P516

    • NAID

      130007644145

    • ISSN
      0910-6340, 1348-2246
    • Year and Date
      2019-05-10
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Electrochemical determination with a long-length carbon nanotube electrode of quercetin glucosides in onion, apple peel, and tartary buckwheat2019

    • Author(s)
      S. Takahashi, H. Muguruma, N. Osakabe, H. Inoue, T. Ohsawa
    • Journal Title

      Food Chemistry

      Volume: 300 Pages: 125189-125189

    • DOI

      10.1016/j.foodchem.2019.125189

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Diameter dependence of single-walled carbon nanotubes with flavin adenine dinucleotide glucose dehydrogenase for direct electron transfer bioanodes2019

    • Author(s)
      A. Suzuki, K. Ishida, H. Muguruma, H. Iwasa, T. Tanaka, A. Hiratsuka, K. Tsuji, T. Kishimoto
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 58 Issue: 5 Pages: 051015-051015

    • DOI

      10.7567/1347-4065/ab14fb

    • NAID

      210000155666

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] ケトン体を検出するバイオセンサ2022

    • Author(s)
      鈴木和志、六車仁志、岩佐尚徳、田中丈士、清水哲夫、平塚淳典、星野陽子、辻勝巳、岸本高英
    • Organizer
      2022年春季第69回応用物理学会学術講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] カーボンナノチューブとフラビンアデニンジヌクレオチドグルコース脱水素酵素を用いる直接電子伝達型バイオセンサストリップ2021

    • Author(s)
      斗米太一、六車仁志、岩佐尚徳、田中丈士、平塚淳典、星野陽子、辻勝巳、岸本高英
    • Organizer
      2021年秋季第82回応用物理学会学術講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] カーボンナノチューブ電極を用いたドーパミンとノルアドレナリンの同時定量2021

    • Author(s)
      関光洋、和田遼太郎、六車仁志
    • Organizer
      2021年秋季第82回応用物理学会学術講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] カーボンナノチューブ電極を用いるコーヒー中のクロロゲン酸類の定量2021

    • Author(s)
      和田遼太郎、松島大秀、六車仁志、越坂部奈緒美
    • Organizer
      2021年秋季第82回応用物理学会学術講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] カーボンナノチューブ/カルボキシメチルセルロース電極を用いるコーヒー焙煎度の判定2021

    • Author(s)
      和田遼太郎、松島大秀、六車仁志、越坂部奈緒美
    • Organizer
      日本分析化学会第70年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ウリカーゼ/カーボンナノチューブ/カルボシキメチルセルロース電極を用いる血中および尿中の尿酸の検出2020

    • Author(s)
      福田冬弥、六車仁志、岩佐尚徳、田中丈士、平塚淳典、清水哲夫、辻勝巳、岸本高英
    • Organizer
      2020年春季第67回応用物理学会学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] Diameter dependence of single-walled carbon nanotube with flavin adenine dinucleotide glucose dehydrogenase for direct electron transfer biosensor2019

    • Author(s)
      A. Suzuki, H. Muguruma, H. Iwasa, T, Tanaka, A. Hiratsuka, K. Tshiji, T. Kishimoto
    • Organizer
      32st International Microprocesses and Nanotechnology Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Electrochemical determination of quercetin glucosides in food with a carbon nanotube electrode2019

    • Author(s)
      S. Takahashi, H. Muguruma, N. Osakabe, H. Inoue, T. Ohsawa
    • Organizer
      32st International Microprocesses and Nanotechnology Conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Simultaneous Electrochemical Determination of Isoquercitrin and Epigallocatechingallate Electrode with A Carbon Nanotube Electrode2019

    • Author(s)
      S. Takahashi, H. Muguruma, N. Osakabe, H. Inoue, T. Ohsawa
    • Organizer
      10th International conference on Molecular Electronics and Bioelectronics(M&BE10)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] A Biological Ink Composed by Glycan Chain Rich Enzyme and Single-Walled Carbon Nanotube/Surfactant Aqueous Solution for Printable Biosensor2019

    • Author(s)
      A. Suzuki, H. Muguruma, H. Iwasa, A. Hiratsuka, H. Uzawa
    • Organizer
      10th International conference on Molecular Electronics and Bioelectronics(M&BE10)
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] カーボンナノチューブ電極を用いる食品中のケルセチン配糖体の定量2019

    • Author(s)
      高橋翔太、六車仁志、越阪部奈緒美、井上均、大澤達也
    • Organizer
      2019年秋季第80回応用物理学会学術講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] フラビンアデニンジヌクレオチドグルコース脱水素酵素と単層カーボンナノチューブからなる直接電子伝達バイオセンサの直径依存性2019

    • Author(s)
      鈴木敦哉、六車仁志、岩佐尚徳、田中丈士、平塚淳典、辻勝巳、岸本高英
    • Organizer
      2019年秋季第80回応用物理学会学術講演会
    • Related Report
      2019 Research-status Report
  • [Remarks] 研究者データベース

    • URL

      https://shibaura.pure.elsevier.com/en/persons/hitoshi-muguruma

    • Related Report
      2019 Research-status Report
  • [Patent(Industrial Property Rights)] 酵素ー電極間電子伝達増強作用の有無と予測方法2019

    • Inventor(s)
      岩佐尚徳、平塚淳典、六車仁志、他
    • Industrial Property Rights Holder
      東洋紡株式会社、国立研究開発法人産業技術総合研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Acquisition Date
      2021
    • Related Report
      2021 Annual Research Report

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Published: 2019-04-18   Modified: 2023-01-30  

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