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Fabrication of new cartilage tissues using latex nanoparticles as reinforcing particles and enhancing factors of cell differentiation

Research Project

Project/Area Number 19K05701
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 37010:Bio-related chemistry
Research InstitutionToyota Technological Institute

Principal Investigator

OKAMOTO MASAMI  豊田工業大学, 工学(系)研究科(研究院), 特任准教授 (60288553)

Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsラテックス / 軟骨再生 / 生体コンポジット / 細胞機能 / 薬理効果 / 3次元培養 / マイクロインデンテーション / 弾性率 / 軟骨細胞集塊 / 組織学的評価 / 遺伝子解析 / 免疫染色 / ヒト間葉系幹細胞 / 細胞分裂周期 / ヘマトキシリン・エオジン染色 / 軟骨組織 / 細胞分化 / 補強ナノ粒子
Outline of Research at the Start

軟骨の再生は重要かつ緊急の課題であり、臨床的にもその必要性は極めて高いために、インビトロ培養による軟骨組織形成の研究が注目されている。細胞増殖因子をバランスよく組み合わせた幹細胞からの軟骨組織の再生では、新しく形成された軟骨組織が繊維状コラーゲンを産生するために力学特性が劣り、力学的強度の向上が大きな課題となっている。ラテックスナノ粒子がヒト間葉系幹細胞の軟骨分化誘導を促進させる効果が見出されたことから、ラテックスナノ粒子を補強粒子かつ細胞分化の増強因子として作用せることで、天然の軟骨細胞外マトリックス組成物を模倣し、同様の力学的特性を特徴とする優れた硝子軟骨組織を創成することを目指す。

Outline of Final Research Achievements

we conducted a precise structural analysis of natural rubber latex (NRL)nanoparticles, and based on the cytotoxicity research of NRL particles, we demonstrated that target
selectivity and programmed cell death were induced in cancer cells. , found pharmacological effects as anticancer activity. He developed NRL particles as novel bioactive substances that can modulate various cellular functions (cell adhesion, differentiation, signaling pathways, etc.) and evaluated the physicochemical interactions between NRL particles and cells. His recent advances include research on the induction of bone and cartilage differentiation by NRL particles, and he was the first to successfully create a bio-composite incorporating NRL nanoparticles.

Academic Significance and Societal Importance of the Research Achievements

高分子ナノコンポジット研究を基盤として生体組織工学に向けた「生体コンポジット」という新しい研究領域をつくることに挑戦した。軟骨の再生は重要かつ緊急の課題であり、臨床的にもその必要性は極めて高いために、in vitro培養による軟骨組織形成の研究が注目されている。NRLの薬理的用途としての可能性は高いものの,NRLナノ粒子の細胞毒性に関しては十分に理解されてこなかった。そこで申請者はNRL粒子表面のタンパク質にhMSCの増殖や軟骨細胞への分化に効果があることを、マーカー遺伝子解析によって見いだした。さまざまな細胞機能を調節できる新規生物活性物質としてのNRLナノ粒子の開発に成功した。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (19 results)

All 2024 2023 2022 2020 2019 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (13 results) (of which Int'l Joint Research: 9 results,  Invited: 9 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells in hypoxia2024

    • Author(s)
      Yuki Okamoto, Masaya Kinoshita, Masami Okamoto
    • Journal Title

      Nanocomposites

      Volume: 6 Issue: 4 Pages: 137-148

    • DOI

      10.1080/20550324.2020.1851438

    • Related Report
      2023 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biocomposites composed of natural rubber latex and cartilage tissue derived from human mesenchymal stem cells2024

    • Author(s)
      Masaya Kinoshita, Yuki Okamoto, Mitsuru Furuya, Masami Okamoto
    • Journal Title

      Materials Today Chemistry

      Volume: 12 Pages: 315-323

    • DOI

      10.1016/j.mtchem.2019.03.002

    • Related Report
      2023 Annual Research Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Biocomposites composed of cartilage and natural rubber latex2023

    • Author(s)
      Yuki Okamoto, Masami Okamoto
    • Journal Title

      Int Phys Med Rehab J

      Volume: 8 Issue: 1 Pages: 40-41

    • DOI

      10.15406/ipmrj.2023.08.00331

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 生体コンポジット2022

    • Author(s)
      岡本正巳
    • Journal Title

      日本ゴム協会誌

      Volume: 6 Pages: 1-4

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] Role of natural rubber latex in tissue engineering2024

    • Author(s)
      M. Okamoto
    • Organizer
      LATEX 2024 Conference,
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells2023

    • Author(s)
      M. Okamoto
    • Organizer
      The 9th International Conference on Chemical and Polymer Engineering (ICCPE2023), London
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells2023

    • Author(s)
      M. Okamoto
    • Organizer
      PPS-37, Fukuoka, Japan
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Cytotoxicity and anticancer activity of natural rubber latex particles for cancer cells2023

    • Author(s)
      M. Okamoto, M Kinoshita
    • Organizer
      International conference on Materials Science Engineering and Technology, Dubai, UAE
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells2022

    • Author(s)
      Masami Okamoto
    • Organizer
      1st State Congress of Students of Science and Engineering of Materials (CEECIM) 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells2022

    • Author(s)
      Masami Okamoto
    • Organizer
      2nd Global Summit on Biomaterials and Applications (GSBA2022)
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Cytotoxicity and anticancer activity of natural rubber latex particles for cancer cells2022

    • Author(s)
      Masami Okamoto, Masaya Kinoshita
    • Organizer
      International conference on Materials Science Engineering and Technology
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Fabrication of cartilage/natural rubber latex biocomposites derived from human mesenchymal stem cells2022

    • Author(s)
      Masami Okamoto
    • Organizer
      International Polymer Processing Society 37
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 低酸素濃度における間葉系幹細胞の軟骨分化:天然ゴムラテックス添加軟骨組織の構造と物性2020

    • Author(s)
      岡本祐樹、岡本正巳
    • Organizer
      日本ゴム協会2020年次大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 低酸素濃度における間葉系幹細胞の軟骨分化2020

    • Author(s)
      岡本祐樹、岡本正巳
    • Organizer
      第32回高分子加工技術討論会
    • Related Report
      2020 Research-status Report
  • [Presentation] 低酸素濃度における間葉系幹細胞の軟骨分化:天然ゴムラテックスの添加効果2019

    • Author(s)
      岡本祐樹、木下雅也、岡本正巳
    • Organizer
      日本ゴム協会2019年年次大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 低酸素濃度における間葉系幹細胞の軟骨分化:天然ゴムラテックス添加軟骨組織の構造と物性2019

    • Author(s)
      木下雅也、岡本祐樹、岡本正巳
    • Organizer
      第30回エラストマー討論会
    • Related Report
      2019 Research-status Report
  • [Presentation] Biocomposites composed of natural rubber latex and cartilage tissue derived from human mesenchymal stem cells2019

    • Author(s)
      M. Okamoto, M. Kinoshita, Y. Okamoto
    • Organizer
      ADVANCES IN POLYMER SCIENCEAND RUBBER TECHNOLOGY 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Book] Handbook of Chemistry Manufacture and Applications of Natural Rubber2023

    • Author(s)
      M. Okamoto
    • Total Pages
      492
    • Publisher
      Elsevier
    • ISBN
      9780128188439
    • Related Report
      2023 Annual Research Report 2020 Research-status Report
  • [Remarks] Welcome to Okamoto Laboratory

    • URL

      https://www.toyota-ti.ac.jp/Lab/Zairyo/5z50/okamoto.htm

    • Related Report
      2020 Research-status Report 2019 Research-status Report

URL: 

Published: 2019-04-18   Modified: 2025-01-30  

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