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Characterization of Mg uptake mechanism utilizing the natural accessions and the ion-beam irradiated mutants

Research Project

Project/Area Number 19K05751
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionThe University of Tokyo

Principal Investigator

Kobayashi Natsuko  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (60708345)

Co-Investigator(Kenkyū-buntansha) 市橋 泰範  国立研究開発法人理化学研究所, バイオリソース研究センター, チームリーダー (20723810)
小堀 峻吾  国立研究開発法人理化学研究所, バイオリソース研究センター, 開発研究員 (20792691)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsマグネシウム / イネ / シロイヌナズナ / MutMap / QTL-seq / 品種間差 / MutMap法 / 低マグネシウム / 次世代シーケンサー / QTL / イオン吸収
Outline of Research at the Start

本研究では、低マグネシウム環境下での葉のマグネシウム濃度が標準品種の75~50%と大きく低下したシロイヌナズナとイネを用い、Mg-28をトレーサーとして用いたマグネシウム吸収速度の測定し、根で発現する遺伝子情報を得た上で、マグネシウム濃度低下の原因遺伝子を特定することで、新規のマグネシウム吸収機構の発見を目指す。

Outline of Final Research Achievements

A long deletion was found on chromosome 1 of the ion-beam irradiated rice mutant with low magnesium concentration in the shoot part. Due to this deletion, two genes are lost in the mutant. So, we have generated knockout mutants, and identified the gene coding a zing finger protein as the causal gene for low magnesium phenotype. This is the first time that a zinc finger protein involved in magnesium dynamics in plants has been identified.
QTL-seq analysis was performed on the genomes of the low-magnesium Arabidopsis variety Ove-0 and the high-magnesium variety Col-0, and the locus that regulates magnesium concentration was found to be located in the 0~2.3 Mb region of chromosome 5.

Academic Significance and Societal Importance of the Research Achievements

多量必須元素であるマグネシウムの吸収や体内動態を司る複数のイオン輸送体は同定されているが、それらが協調してマグネシウムの濃度を制御する仕組みはよくわかっていない。本研究で発見したイネの遺伝子は、微生物や哺乳類にも相同遺伝子が存在するが、これまでにマグネシウム動態への関与が示されたことはない。また、シロイヌナズナにおける候補領域にも、マグネシウムに関係する既知の遺伝子は座上しておらず、マグネシウムの濃度制御に関わる新規の因子の発見が予想される。近年、主要穀物中のマグネシウム濃度は低下の一途をたどっており、これらの遺伝子の情報は高マグネシウム品種の開発につながると期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (10 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (3 results) Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] Fudan University(中国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Universite libre de Bruxelles(ベルギー)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] ブリュッセル自由大学(ベルギー)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Mutagenesis Analysis of GMN Motif in Arabidopsis Mg2+ Transporter AtMRS2-12022

    • Author(s)
      Xiaoyu Yang, Natsuko I. Kobayashi I., Yoshiki Hayashi, Koichi Ito, Yoshitaka Moriwaki, Tohru Terada, Kentaro Shimizu, Motoyuki Hattori, Ren Iwata, Hisashi Suzuki, Tomoko M. Nakanishi, Keitaro Tanoi
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: -

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of the practical method for ion influx, efflux, and net flux measurement in plant roots2022

    • Author(s)
      Natsuko I. KOBAYASHI, Xiaoyu YANG, Ren IWATA, Hisashi SUZUKI, Tomoko M. NAKANISHI, Keitaro TANOI
    • Journal Title

      Radioisotopes

      Volume: -

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Short-Term Magnesium Deficiency Triggers Nutrient Retranslocation in Arabidopsis thaliana2020

    • Author(s)
      Ogura Takaaki、Kobayashi Natsuko I.、Hermans Christian、Ichihashi Yasunori、Shibata Arisa、Shirasu Ken、Aoki Naohiro、Sugita Ryohei、Ogawa Takahiro、Suzuki Hisashi、Iwata Ren、Nakanishi Tomoko M.、Tanoi Keitaro
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 563-563

    • DOI

      10.3389/fpls.2020.00563

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Identification of QTL Regulating Leaf Magnesium Concentration by Using Natural Accessions in Arabidopsis thaliana2022

    • Author(s)
      Akane Kodaka, Natsuko Kobayashi I., Toru Kudo, Misako Kato, Keitaro Tanoi
    • Organizer
      日本植物生理学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] マグネシウム含量が半減するイネ変異体の解析2021

    • Author(s)
      小林 奈通子, 高木 宏樹, 大西 孝幸, 鈴木 寿, 岩田 錬, 市橋 泰範, 横井 彩子, 土岐 精一, 中西 友子, 田野井 慶太朗
    • Organizer
      日本土壌肥料学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 植物の根におけるイオン動態のRIトレーサーによる定量解析2021

    • Author(s)
      小林 奈通子,鈴木 寿,岩田 練, 中西 友子,田野井 慶太朗
    • Organizer
      第58回アイソトープ・放射線研究発表会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Low magnesium conditions rapidly induce high-affinity magnesium transport system in Arabidopsis roots2019

    • Author(s)
      Takaaki Ogura, Natsuko I. Kobayashi, Hisashi Suzuki, Ren Iwata, Tomoko M. Nakanishi and Keitaro Tanoi
    • Organizer
      18th International Workshop on Plant Membrane Biology
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2023-01-30  

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