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Regulation of strigolactone biosynthesis in Arabidopsis

Research Project

Project/Area Number 19K05757
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionEhime University

Principal Investigator

YONEYAMA KAORI  愛媛大学, 農学研究科, 准教授 (20769997)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsストリゴラクトン / シロイヌナズナ / LC-MS/MS / 養分欠乏
Outline of Research at the Start

ストリゴラクトン(SL)は、2008年に、植物の地上部枝分かれを抑制する新奇植物ホルモンとして報告された。その後SLは、根の形態形成、二次成長、葉の老化促進など、植物の生長・分化に深く関与していることが明らかとなり、SL研究の重要性は高まっている。一方、SLは化学的に不安定で植物の生産・分泌量も極めて微量であるため、その生合成経路、生合成・分泌調節メカニズムの詳細には不明な点が多い。また、現在でも植物ホルモンの活性本体は特定されていない。本研究では、シロイヌナズナのSL生合成を促進する条件を見出し、シロイヌナズナにおけるSL生合成調節メカニズムの解明を目指す。

Outline of Final Research Achievements

In Arabidopsis thaliana (Col-0), nitrogen deficiency significantly promoted the expression of strigolactone (SL) biosynthetic genes. In Atd14 mutants, carlactone and methyl carlactonoate, major SLs of Arabidopsis, accumulated under nitrogen deficiency. Furthermore, the expression of MAX1, which is involved in the conversion of carlactone into carlactonoic acid, increased within 3 hours after beginning of light period. These results suggest that for successful SL detection/quantification in Arabidopsis, it is better to grow plants under nitrogen deficiency and harvest them within 3 hours after beginning of light period.

Academic Significance and Societal Importance of the Research Achievements

植物の生長において重要な二次代謝産物であるストリゴラクトンを、実際の農業生産に利用する研究の重要性が高まっている。しかしストリゴラクトンは、化学的に不安定で壊れやすく、植物の生産・分泌量が微量であるため、その生合成経路、生合成・調節メカニズムの詳細については不明な点が多い。また植物ホルモンとしての活性本体も特定されていない。本研究では、モデル植物のシロイヌナズナのストリゴラクトン生合成を促進する条件を見出すことに成功した。今後、その条件下で豊富に存在するシロイヌナズナの変異体を栽培し、ストリゴラクトンの解析を進めることにより生合成調節メカニズムの解明につながることが期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (26 results)

All 2022 2021 2020 2019

All Journal Article (10 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 10 results,  Open Access: 5 results) Presentation (14 results) (of which Int'l Joint Research: 5 results,  Invited: 10 results) Book (2 results)

  • [Journal Article] Germination stimulant activity of isothiocyanates on Phelipanche spp2022

    • Author(s)
      Hinako Miura 、Ryota Ochi、Hisashi Nishiwaki、Satoshi Yamauchi、Xiaonan Xie、Hidemitsu Nakamura、Koichi Yoneyama、Kaori Yoneyama
    • Journal Title

      Plants

      Volume: 24 Issue: 5 Pages: 606-606

    • DOI

      10.3390/plants11050606

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Strigolactone biosynthesis catalyzed by cytochrome P450 and sulfotransferase in sorghum2021

    • Author(s)
      Yoda Akiyoshi、Mori Narumi、Akiyama Kohki、Kikuchi Mayu、Xie Xiaonan、Miura Kenji、Yoneyama Kaori、Sato‐Izawa Kanna、Yamaguchi Shinjiro、Yoneyama Koichi、Nelson David C.、Nomura Takahito
    • Journal Title

      New Phytologist

      Volume: 232 Issue: 5 Pages: 1999-2010

    • DOI

      10.1111/nph.17737

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Strigolactones, how are they synthesized to regulate plant growth and development?2021

    • Author(s)
      Yoneyama Kaori、Brewer Philip B
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 63 Pages: 102072-102072

    • DOI

      10.1016/j.pbi.2021.102072

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structure?activity relationship of the aromatic moiety of 6-substituted 5,6-dihydro-2<i>H</i>-pyran-2-one to find the novel compound showing higher plant growth inhibitory activity2021

    • Author(s)
      Ochi Ryota、Yoneyama Kaori、Nishiwaki Hisashi、Yamauchi Satoshi
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 86 Issue: 2 Pages: 165-169

    • DOI

      10.1093/bbb/zbab185

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation and Quantification of Natural Strigolactones from Root Exudates2021

    • Author(s)
      Xie Xiaonan、Yoneyama Kaori、Nomura Takahito、Yoneyama Koichi
    • Journal Title

      Strigolactones. Methods in Molecular Biology

      Volume: 2309 Pages: 3-12

    • DOI

      10.1007/978-1-0716-1429-7_1

    • ISBN
      9781071614280, 9781071614297
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hydroxyl carlactone derivatives are predominant strigolactones in Arabidopsis2020

    • Author(s)
      Yoneyama Kaori、Akiyama Kohki、Brewer Philip B.、Mori Narumi、Kawano‐Kawada Miyuki、Haruta Shinsuke、Nishiwaki Hisashi、Yamauchi Satoshi、Xie Xiaonan、Umehara Mikihisa、Beveridge Christine A.、Yoneyama Koichi、Nomura Takahito
    • Journal Title

      Plant Direct

      Volume: 4 Issue: 5 Pages: 1-14

    • DOI

      10.1002/pld3.219

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Do phosphate and cytokinin interact to regulate strigolactone biosynthesis or act independently?2020

    • Author(s)
      Kaori Yoneyama et al
    • Journal Title

      frontiers in Plant Science

      Volume: in press Pages: 1-9

    • DOI

      10.3389/fpls.2020.00438

    • Related Report
      2020 Research-status Report 2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chemical identification of 18-hydroxycarlactonoic acid as an LjMAX1 product and in planta conversion of its methyl ester to canonical and non-canonical strigolactones in Lotus japonicus2020

    • Author(s)
      Mori Narumi、Sado Aika、Xie Xiaonan、Yoneyama Kaori、Asami Kei、Seto Yoshiya、Nomura Takahito、Yamaguchi Shinjiro、Yoneyama Koichi、Akiyama Kohki
    • Journal Title

      Phytochemistry

      Volume: 174 Pages: 112349-112365

    • DOI

      10.1016/j.phytochem.2020.112349

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Regulation of biosynthesis, perception, and functions of strigolactones for promoting arbuscular mycorrhizal symbiosis and managing root parasitic weeds2019

    • Author(s)
      Yoneyama Koichi、Xie Xiaonan、Yoneyama Kaori、Nomura Takahito、Takahashi Ikuo、Asami Tadao、Mori Narumi、Akiyama Kohki、Kusajima Miyuki、Nakashita Hideo
    • Journal Title

      Pest Management Science

      Volume: 2 Issue: 9 Pages: 200-205

    • DOI

      10.1002/ps.5401

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Lotuslactone, a non-canonical strigolactone from Lotus japonicus2019

    • Author(s)
      Xie Xiaonan、Mori Narumi、Yoneyama Kaori、Nomura Takahito、Uchida Kenichi、Yoneyama Koichi、Akiyama Kohki
    • Journal Title

      Phytochemistry

      Volume: 157 Pages: 200

    • DOI

      10.1016/j.phytochem.2018.10.034

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] ストリゴラクトン生産・分泌の環境応答2021

    • Author(s)
      米山香織
    • Organizer
      日本植物生理学会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 根圏に分泌されるストリゴラクトンの新たな機能とその分泌制御メカニズム2021

    • Author(s)
      米山香織
    • Organizer
      日本植物学会第85回大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] The Possibility of Strigolactones for Plant-Plant Communication2021

    • Author(s)
      Kaori Yoneyama
    • Organizer
      3rd International Congress on Strigolactones
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Strigolactones, how are they synthesized to regulate plant growth and development?2021

    • Author(s)
      Kaori Yoneyama
    • Organizer
      The fourth webinar of the IRN France-Japan Frontiers in Plant Biology on “Photosynthesis and Metabolism”
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] イソチオシアネートによる根寄生雑草の発芽刺激活性2021

    • Author(s)
      三浦妃奈子、米山香織
    • Organizer
      2021 年度日本農芸化学会西日本・中四国・関西支部合同大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Do rice plants sense SLs exuded in the rhizosphere to regulate SL production?2019

    • Author(s)
      Kaori YONEYAMA
    • Organizer
      The 23rd International Conference on Plant Growth Substances
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characterization of germination stimulants fro P. ramosa.2019

    • Author(s)
      Kaori YONEYAMA
    • Organizer
      The 15th World congress on Parasitic Plants
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characterization of parasitic plant-host plant relationship2019

    • Author(s)
      Kaori YONEYAMA
    • Organizer
      Plant health and quality, summer school in France
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 寄生および共生をコントロールする植物化学調節物質2019

    • Author(s)
      米山香織
    • Organizer
      日本農芸化学会中国支部市民フォーラム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] How are strigolactone production and exudation regulated in rice plants?2019

    • Author(s)
      Kaori YONEYAMA
    • Organizer
      nternational Symposium on the Future Direction of Plant Science by Young Researchers
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 新奇植物ホルモン-ストリゴラクトン-の農業応用への可能性2019

    • Author(s)
      米山香織
    • Organizer
      愛媛県農林水産研究所合同研修会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Strigolactone Story2019

    • Author(s)
      米山香織
    • Organizer
      農芸化学会中国四国支部若手シンポジウム
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Regulation mechanism of strigolactone production in rice plants2019

    • Author(s)
      Kaori YONEYAMA
    • Organizer
      Workshop on Parasitic Plant and Strigolactone
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] イネのストリゴラクトン生産・分泌制御メカニズム2019

    • Author(s)
      米山香織
    • Organizer
      植物化学調節学会
    • Related Report
      2019 Research-status Report
  • [Book] Engineering Plant for Agriculture2019

    • Author(s)
      Kaori YONEYAMA
    • Total Pages
      231
    • Publisher
      Cold spring Harbor Perspectives in Biology
    • ISBN
      9781621823124
    • Related Report
      2019 Research-status Report
  • [Book] アグリバイオ2019

    • Author(s)
      米山香織
    • Total Pages
      102
    • Publisher
      北隆館
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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