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Explotiation of probiotic system capable of reducing peroxides

Research Project

Project/Area Number 19K05780
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionTokyo University of Agriculture

Principal Investigator

Niimura Youichi  東京農業大学, 生命科学部, 教授 (00180563)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords過酸化脂肪酸還元乳酸菌 / プロバイオティックス / 過酸化脂肪酸 / 過酸化物還元 / 抗酸化ストレス / 乳酸菌 / 過酸化脂肪酸還元酵素 / 酸化ストレス防御機能 / 酸化ストレス / プロバイオティクス / 過酸化水素 / 過酸化物還元乳酸菌 / Lactobacillus plantarum / glutathione reductase / グルタチオン / グルタチオン還元酵素 / Glutathione reductase / 過酸化脂質
Outline of Research at the Start

過酸化脂質は、疾病原因として最も注目されている過酸化物で、それを構成する過酸化脂肪酸の還元機構解明は重要課題である。そこで、過酸化脂肪酸還元乳酸菌から過酸化脂肪酸還元酵素を精製し酵素の反応機構解明により酸化ストレス防御機能の安全性を確証したい。
一方、H2O2は、鉄還元が起きやすい腸内環境では、最強毒性・OHを発生するため、H2O2還元力も重要となる。そこで、過酸化脂肪酸還元乳酸菌とH2O2分解乳酸菌の単一菌株での過酸化物還元活性培養条件を確立し、さらに両還元活性 の高い複合培養条件を確立する。この培養菌体を用いて高い酸化ストレス防御力を持つプロバイオティックス乳酸菌を完成させたい。

Outline of Final Research Achievements

Reinforcement of hydroperoxide-eliminating activity in the intestines and colon should prevent associated diseases. We successfully isolated an alternative lactic acid bacterium, Lactobacillus plantarum P1-2, that can efficiently reduce environmental fatty acid hydroperoxides to hydroxy derivatives through a 2-electron reduction. The strain exhibited a wide concentration range with regard to the environmental reducing activity for hydroperoxide. Given this, the isolate orally administered to the oxygen-sensitive short-lived nematode mutant, and this resulted in a significant expansion of its lifespan. This observation suggests that L. plantarum P1-2 inhibit internal oxidative stress. To determine the specific organs involved in this response, we performed a similar experiment in rats, involving induced lipid peroxidation by iron-overloading. We observed that only L. plantarum P1-2 inhibited colonic mucosa lipid peroxidation in rats with induced oxidative stress.

Academic Significance and Societal Importance of the Research Achievements

腸には疾病防御の要であり、過酸化物は各種疾病の原因物質であるが、腸の過酸化物分解活性は通常組織より低い。従って、積極的に菌体外過酸化脂肪酸を還元できる乳酸菌が分離できれば、その生菌剤による腸内疾患の防止や治療の可能性が期待されるが、研究報告は見いだせない。
私達は、目的の乳酸菌、Lactobacillus plantarum P1-2株の分離に成功し、線虫の酸素感受性短寿命突然変異体投与では、酸化ストレス防御効果が認められた。そこで、ラットに供試したところ、乳酸菌投与群に大腸粘膜中の脂質過酸化指標値の低下を認めた。乳酸菌による生体内過酸化脂肪酸還元は学術的、応用面での発展が期待される。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (11 results)

All 2022 2020 2019

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (5 results)

  • [Journal Article] Esterification of side-chain oxysterols by lysosomal phospholipase A22020

    • Author(s)
      Abe Akira、Hiraoka Miki、Matsuzawa Fumiko、Aikawa Sei-ichi、Niimura Youichi
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids

      Volume: 1865 Issue: 10 Pages: 158787-158787

    • DOI

      10.1016/j.bbalip.2020.158787

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Free Flavin Participates in Iron and Also Oxygen Metabolism in Bacteria2020

    • Author(s)
      Tomonori Suzuki, Shinya Kimata, Junichi Satoh, Kouji Takeda, Daichi Mochizuki, Ken Kitano, Akio Watanabe, Etsuo Yoshimura, Masataka Uchino, Shinji Kawasaki, Akira Abe, Youichi Niimura
    • Journal Title

      Journal of Bacteriology & Parasitology

      Volume: 11 Pages: 1-7

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats2020

    • Author(s)
      Akio Watanabe, Takuro Yamaguchi, Kaeko Murota, Naoaki Ishi, Junji Terao, Sanae Okada, Naoto Tanaka,, Shinya Kimata, Akira Abe, Tomonori Suzuki, Masataka Uchino Youichi Niimura
    • Journal Title

      PLOS ONE

      Volume: 15(2) Pages: 1-18

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Atomic force microscopic image data of botulinum neurotoxin complexes with different molecular sizes2019

    • Author(s)
      Shin-Ichiro Miyashita , Yoshimasa Sagane , Takamasa Uchino , Shura Karatsu , Keita Hosoya , Ihsun Huang , Koichi Niwa , Toshihiro Watanabe, Yoichi Niimura , Tomonori Suzuki ,
    • Journal Title

      Data in brief

      Volume: 25 Pages: 1-5

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Free flavins accelerate release of ferrous iron from iron storage proteins by both free flavin-dependent and -independent ferric reductases in <i>Escherichia coli</i>2019

    • Author(s)
      Junichi Satoh, Shinya Kimata,, Shota Nakamoto, Tatsuya Ishii, Eisuke Tanaka, Sayuri Yumoto, Kouji Takeda, Etsuro Yoshimura, Yu Kanesaki, Taichiro Ishige, Keisuke Tanaka, Akira Abe, Shinji Kawasaki, and Youichi Niimura
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 65 Issue: 6 Pages: 308-315

    • DOI

      10.2323/jgam.2019.03.001

    • NAID

      130007792548

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comparison of composition-gradient sedimentation equilibrium and composition-gradient static light scattering as techniques for quantitative characterization of biomolecular interactions: A case study2019

    • Author(s)
      Fumio Arisak, Youichi Niimur, Allen P. Mintonc,
    • Journal Title

      Analytical Biochemistry

      Volume: 583 Pages: 1-9

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 過酸化脂肪酸を還元するLactobacillus plantarum P1-2株によるプロバイオティクス資材の開発2022

    • Author(s)
      鈴木智典、糸田侑花、野口龍生、植松悠、石塚雄一、村崎友彦、渡邊昭夫、野村佳歩、阿部晃、新村洋一、内野昌孝
    • Organizer
      日本農芸化学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] L. plantarum P1-2株の酸化ストレス防御機構に関する研究2022

    • Author(s)
      鈴木智典、奥山貴人、相本健吾、松永一太、渡邊昭夫、武田晃治、野村佳歩、阿部晃、新村洋一、内野昌孝
    • Organizer
      日本農芸化学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 酸素耐性嫌気性菌 Amphibacillus xylanus と通性好気性菌E.coli における“嫌気ストレス”に関する研究2022

    • Author(s)
      鈴木智典、加藤紗弥、木俣真弥、酒井陽一、高山努、中本忠宏、中島聖也、福島多一、武田晃治、吉村悦郎、野村佳歩、阿部晃、新村洋一、内野昌孝
    • Organizer
      日本農芸化学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 過酸化脂肪酸還元乳酸菌の酸化ストレス防御機構2022

    • Author(s)
      鈴木智典、奥山貴人、松永一太、相本健吾、渡邊昭夫、武田晃治、野村佳歩、阿部晃、新村洋一、内野昌孝
    • Organizer
      日本食品補蔵学会
    • Related Report
      2021 Research-status Report
  • [Presentation] O2耐性菌 Amphibacillus xylanus の“嫌気ストレス”に関する研究[第2報]2020

    • Author(s)
      大野 由依、木俣 真弥、○齋藤 迅成、松本 滉平、尾崎 和帆、川越 樹、田中 啓介、酒井 陽一、高山 努、中本 忠宏、武田 晃治、吉村 悦郎、阿部 晃、川崎 信治、新村 洋一
    • Organizer
      2020, 3月 日本農芸化学会大会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2024-12-25  

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