Explotiation of probiotic system capable of reducing peroxides
Project/Area Number |
19K05780
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 38020:Applied microbiology-related
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Research Institution | Tokyo University of Agriculture |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Project Status |
Completed (Fiscal Year 2022)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | 過酸化脂肪酸還元乳酸菌 / プロバイオティックス / 過酸化脂肪酸 / 過酸化物還元 / 抗酸化ストレス / 過酸化水素 / 過酸化物還元乳酸菌 / 過酸化脂肪酸還元酵素 / 乳酸菌 / Lactobacillus plantarum / glutathione reductase / 酸化ストレス / グルタチオン / グルタチオン還元酵素 / Glutathione reductase / 過酸化脂質 / プロバイオティクス |
Outline of Research at the Start |
過酸化脂質は、疾病原因として最も注目されている過酸化物で、それを構成する過酸化脂肪酸の還元機構解明は重要課題である。そこで、過酸化脂肪酸還元乳酸菌から過酸化脂肪酸還元酵素を精製し酵素の反応機構解明により酸化ストレス防御機能の安全性を確証したい。 一方、H2O2は、鉄還元が起きやすい腸内環境では、最強毒性・OHを発生するため、H2O2還元力も重要となる。そこで、過酸化脂肪酸還元乳酸菌とH2O2分解乳酸菌の単一菌株での過酸化物還元活性培養条件を確立し、さらに両還元活性 の高い複合培養条件を確立する。この培養菌体を用いて高い酸化ストレス防御力を持つプロバイオティックス乳酸菌を完成させたい。
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Outline of Final Research Achievements |
Reinforcement of hydroperoxide-eliminating activity in the intestines and colon should prevent associated diseases. We successfully isolated an alternative lactic acid bacterium, Lactobacillus plantarum P1-2, that can efficiently reduce environmental fatty acid hydroperoxides to hydroxy derivatives through a 2-electron reduction. The strain exhibited a wide concentration range with regard to the environmental reducing activity for hydroperoxide. Given this, the isolate orally administered to the oxygen-sensitive short-lived nematode mutant, and this resulted in a significant expansion of its lifespan. This observation suggests that L. plantarum P1-2 inhibit internal oxidative stress. To determine the specific organs involved in this response, we performed a similar experiment in rats, involving induced lipid peroxidation by iron-overloading. We observed that only L. plantarum P1-2 inhibited colonic mucosa lipid peroxidation in rats with induced oxidative stress.
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Academic Significance and Societal Importance of the Research Achievements |
腸には疾病防御の要であり、過酸化物は各種疾病の原因物質であるが、腸の過酸化物分解活性は通常組織より低い。従って、積極的に菌体外過酸化脂肪酸を還元できる乳酸菌が分離できれば、その生菌剤による腸内疾患の防止や治療の可能性が期待されるが、研究報告は見いだせない。 私達は、目的の乳酸菌、Lactobacillus plantarum P1-2株の分離に成功し、線虫の酸素感受性短寿命突然変異体投与では、酸化ストレス防御効果が認められた。そこで、ラットに供試したところ、乳酸菌投与群に大腸粘膜中の脂質過酸化指標値の低下を認めた。乳酸菌による生体内過酸化脂肪酸還元は学術的、応用面での発展が期待される。
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Free Flavin Participates in Iron and Also Oxygen Metabolism in Bacteria2020
Author(s)
Tomonori Suzuki, Shinya Kimata, Junichi Satoh, Kouji Takeda, Daichi Mochizuki, Ken Kitano, Akio Watanabe, Etsuo Yoshimura, Masataka Uchino, Shinji Kawasaki, Akira Abe, Youichi Niimura
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Journal Title
Journal of Bacteriology & Parasitology
Volume: 11
Pages: 1-7
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Isolation of lactic acid bacteria capable of reducing environmental alkyl and fatty acid hydroperoxides, and the effect of their oral administration on oxidative-stressed nematodes and rats2020
Author(s)
Akio Watanabe, Takuro Yamaguchi, Kaeko Murota, Naoaki Ishi, Junji Terao, Sanae Okada, Naoto Tanaka,, Shinya Kimata, Akira Abe, Tomonori Suzuki, Masataka Uchino Youichi Niimura
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Journal Title
PLOS ONE
Volume: 15(2)
Pages: 1-18
Related Report
Peer Reviewed / Open Access
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[Journal Article] Atomic force microscopic image data of botulinum neurotoxin complexes with different molecular sizes2019
Author(s)
Shin-Ichiro Miyashita , Yoshimasa Sagane , Takamasa Uchino , Shura Karatsu , Keita Hosoya , Ihsun Huang , Koichi Niwa , Toshihiro Watanabe, Yoichi Niimura , Tomonori Suzuki ,
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Journal Title
Data in brief
Volume: 25
Pages: 1-5
Related Report
Peer Reviewed / Open Access
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[Journal Article] Free flavins accelerate release of ferrous iron from iron storage proteins by both free flavin-dependent and -independent ferric reductases in <i>Escherichia coli</i>2019
Author(s)
Junichi Satoh, Shinya Kimata,, Shota Nakamoto, Tatsuya Ishii, Eisuke Tanaka, Sayuri Yumoto, Kouji Takeda, Etsuro Yoshimura, Yu Kanesaki, Taichiro Ishige, Keisuke Tanaka, Akira Abe, Shinji Kawasaki, and Youichi Niimura
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Journal Title
The Journal of General and Applied Microbiology
Volume: 65
Issue: 6
Pages: 308-315
DOI
NAID
ISSN
0022-1260, 1349-8037
Related Report
Peer Reviewed
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