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AGP degradative mechanism resolved by novel arabinobiose releasing enzymes

Research Project

Project/Area Number 19K05816
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38030:Applied biochemistry-related
Research InstitutionKagoshima University

Principal Investigator

Fujita Kiyotaka  鹿児島大学, 農水産獣医学域農学系, 准教授 (20381189)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2020: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsアラビノガラクタン・プロテイン / ビフィズス菌 / 糖質分解酵素 / AGP / L-アラビノース / 代謝
Outline of Research at the Start

アラビノガラクタン・プロテイン(AGP)は私たちが日常的に摂取する穀物・野菜・果物に含まれる食物繊維の一種であり、ビフィズス菌を増やすプレバイオティクス効果があることが知られている。しかし、ビフィズス菌がどのようにAGPを分解して増えるのかというメカニズムは分かっていなかった。本研究課題では、ビフィズス菌が持つ糖質分解酵素に着目し、AGP分解代謝システムの全容を明らかにすることを目的とする。

Outline of Final Research Achievements

We have been shown that a disaccharide at the end of the side chain of arabinogalactan protein (AGP) was released by a enzyme GAfase from Bifidobacterium longum and the enzyme had the key role for the gum arabic degradating ability. Therefore, we focused on the homologous enzyme gene conserved in other Bifidobacterium species and analyzed its function. As a result, we were able to show that the enzyme from B. pseudocatenulatum is an enzyme AAfase involved in arabinobiose release conserved in AGPs. We were able to clarify a part of the AGP-degradative mechanism on bifidobacteria.

Academic Significance and Societal Importance of the Research Achievements

アラビノガラクタンプロテイン(AGP)は全ての野菜や穀物に含まれる細胞壁構成成分の一つであり、私たちは食物繊維として日常的に摂取している。資化性試験を行うだけでビフィズス菌がプレバイオティクスとしてAGPを利用して増えることは分かるが、分解酵素を同定しなければその仕組みを理解することはできない。今回、AGPに一般的に見られる側鎖構造であるアラビノビオースを遊離する酵素を成人型ビフィズス菌B. pseudocatenulatumに保存されていることを明らかにできた。これは、食物繊維としてAGPを含む野菜や穀物を摂取する意義を論理的に説明することができるようになるという意義がある。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (19 results)

All 2022 2021 2020 2019 Other

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 4 results) Presentation (7 results) (of which Int'l Joint Research: 3 results,  Invited: 3 results) Remarks (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Mechanism of Cooperative Degradation of Gum Arabic Arabinogalactan Protein by Bifidobacterium longum Surface Enzymes2022

    • Author(s)
      Sasaki Yuki、Komeno Masahiro、Ishiwata Akihiro、Horigome Ayako、Odamaki Toshitaka、Xiao Jin-Zhong、Tanaka Katsunori、Ito Yukishige、Kitahara Kanefumi、Ashida Hisashi、Fujita Kiyotaka
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 88 Issue: 6

    • DOI

      10.1128/aem.02187-21

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two α-l-arabinofuranosidases from Bifidobacterium longum subsp. longum are involved in arabinoxylan utilization2022

    • Author(s)
      Komeno Masahiro、Yoshihara Yuki、Kawasaki Junya、Nabeshima Wataru、Maeda Koshi、Sasaki Yuki、Fujita Kiyotaka、Ashida Hisashi
    • Journal Title

      Applied Microbiology and Biotechnology

      Volume: 106 Issue: 5-6 Pages: 1957-1965

    • DOI

      10.1007/s00253-022-11845-x

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a GH36 α-D-Galactosidase Associated with Assimilation of Gum Arabic in <i>Bifidobacterium longum </i>subsp.<i> longum </i>JCM70522021

    • Author(s)
      Sasaki Yuki、Uchimura Yumi、Kitahara Kanefumi、Fujita Kiyotaka
    • Journal Title

      Journal of Applied Glycoscience

      Volume: 68 Issue: 2 Pages: 47-52

    • DOI

      10.5458/jag.jag.JAG-2021_0004

    • NAID

      130008050923

    • ISSN
      1344-7882, 1880-7291
    • Year and Date
      2021-06-11
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Substrate complex structure, active site labeling and catalytic role of the zinc ion in cysteine glycosidase2021

    • Author(s)
      Maruyama Shun、Sawano Kota、Amaki Satoko、Suzuki Takehiro、Narita Satoru、Kimura Kenta、Arakawa Takatoshi、Yamada Chihaya、Ito Yukishige、Dohmae Naoshi、Fujita Kiyotaka、Ishiwata Akihiro、Fushinobu Shinya
    • Journal Title

      Glycobiology

      Volume: 32 Issue: 2 Pages: 171-180

    • DOI

      10.1093/glycob/cwab103

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Identification of difructose dianhydride I synthase/hydrolase from an oral bacterium establishes a novel glycoside hydrolase family2021

    • Author(s)
      Kashima Toma、Okumura Kouki、Ishiwata Akihiro、Kaieda Machika、Terada Tohru、Arakawa Takatoshi、Yamada Chihaya、Shimizu Kentaro、Tanaka Katsunori、Kitaoka Motomitsu、Ito Yukishige、Fujita Kiyotaka、Fushinobu Shinya
    • Journal Title

      Journal of Biological Chemistry

      Volume: 297 Issue: 5 Pages: 101324-101324

    • DOI

      10.1016/j.jbc.2021.101324

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of a novel 3-O-α-D-galactosyl-α-L-arabinofuranosidase for the assimilation of gum arabic AGP in Bifidobacterium longum subsp. longum2021

    • Author(s)
      Sasaki Yuki、Horigome Ayako、Odamaki Toshitaka、Xiao Jin-Zhong、Ishiwata Akihiro、Ito Yukishige、Kitahara Kanefumi、Fujita Kiyotaka
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 87 Issue: 10 Pages: 1-20

    • DOI

      10.1128/aem.02690-20

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Degradation of plant arabinogalactan proteins by intestinal bacteria: characteristics and functions of the enzymes involved.2019

    • Author(s)
      Fujita, K., Sasaki, Y. & Kitahara, K.
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 103 Issue: 18 Pages: 7451-7457

    • DOI

      10.1007/s00253-019-10049-0

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 食物繊維が育む“大人”のビフィズス菌2019

    • Author(s)
      藤田清貴
    • Journal Title

      バイオミディア,生物工学会誌

      Volume: 97 Pages: 736-736

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] D-arabinan degradative enzymes of Microbacterium arabinogalactanolyticum for the assimilation of mycobacterial lipoarabinomannan.2022

    • Author(s)
      Kiyotaka Fujita
    • Organizer
      Microbial Glycoconjugates and the GlySpace Alliance (MiGGA) Symposium
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 未知の糖質分解酵素探索とビフィズス菌の生存戦略の解明.2021

    • Author(s)
      藤田清貴
    • Organizer
      JBA“未来へのバイオ技術” 勉強会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 結核菌細胞壁を構成するD-アラビナンの分解酵素群の発見.2021

    • Author(s)
      藤田清貴
    • Organizer
      第18回糖鎖科学コンソーシアムシンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] アラビノガラクタンプロテインに対する成人型ビフィズス菌の二糖遊離酵素の機能解析2020

    • Author(s)
      佐々木 優紀、柳田真言、吉田圭祐、小田巻 俊孝、清水(肖) 金忠、北原 兼文、藤田清貴
    • Organizer
      日本応用糖質科学会2020年度大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Discovery and characterization of a key enzyme for the assimilation of gum arabic AGP in Bifidobacterium longum subsp. longum JCM7052.2019

    • Author(s)
      Sasaki, Y., Horigome, A., Odamaki, T., Jin-Zhong Xiao, Kitahara, K., Fujita, K..
    • Organizer
      13th International Scientific Conference on Probiotics, Prebiotics, Gut Mirobiota and Health (IPC2019).
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Characterization of mycobacterial arabinan degrading enzymes from Microbacterium arabinogalactanolyticum2019

    • Author(s)
      Fujita, K., Ishiwata, A., Fushinobu, S., Suda.Y., Ito, Y., and Kitahara, K.
    • Organizer
      Japanese-American Symposium on Applied & Translational Glycosciences, ACS Fall 2019 National Meeting and Exposition, San Diego, U.S.A.
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] アラビアガム資化性を持つビフィズス菌由来のGH36 α-D-galactosidaseの機能解析.2019

    • Author(s)
      佐々木 優紀、内村 祐美、堀米 綾子、小田巻 俊孝、清水(肖) 金忠、北原 兼文、藤田清貴
    • Organizer
      日本応用糖質科学会2019年度大会
    • Related Report
      2019 Research-status Report
  • [Remarks]

    • URL

      https://hypba2.jimdo.com

    • Related Report
      2021 Annual Research Report
  • [Remarks] 藤田清貴のHP

    • URL

      https://hypba2.jimdo.com

    • Related Report
      2020 Research-status Report
  • [Patent(Industrial Property Rights)] 糖質分解酵素、抗酸菌の検出キット及び抗酸菌の検出方法.2021

    • Inventor(s)
      藤田清貴
    • Industrial Property Rights Holder
      藤田清貴
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-147456
    • Filing Date
      2021
    • Related Report
      2021 Annual Research Report
  • [Patent(Industrial Property Rights)] 糖質分解酵素、食品添加用細菌のスクリーニング方法、プレバイオティクスの評価方法及びプライマー対2019

    • Inventor(s)
      藤田清貴、石渡明弘
    • Industrial Property Rights Holder
      藤田清貴、石渡明弘
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2020-055336
    • Filing Date
      2019
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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