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Development of Highly Efficient Food Sterilization Technology by Plasma-Based Ion Implantation Using Composite Gases

Research Project

Project/Area Number 19K05903
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 38050:Food sciences-related
Research InstitutionHiroshima Institute of Technology

Principal Investigator

Kakugawa Koji  広島工業大学, 生命学部, 教授 (60441507)

Co-Investigator(Kenkyū-buntansha) 田中 武  広島工業大学, 工学部, 教授 (10197444)
Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords応用微生物学 / 食品加工 / 非加熱殺菌 / プラズマ処理 / プラズマベースイオン注入法 / 殺菌 / HHOガス / PBII法 / プラズマベース注入法 / 耐熱性芽胞菌 / プラズマ / 食品 / 芽胞菌
Outline of Research at the Start

本研究は、複合ガスを用いて、効率的かつ効果的な食品の新規非加熱殺菌技術の開発を目的として実施する。提案者らは、これまで、PBII法を用いて種々の耐熱性芽胞菌の殺菌に適した条件の検討を行ってきた。これまでは、酸素ガスや窒素ガスを用いて殺菌を行ってきたが、本研究では、複合ガスを用いることによって、物理的な処理と化学的な処理を併用した場合の効果的な殺菌条件を特定することを目指す。そして、食品の新規非加熱殺菌技術の開発及び新たな食品の表面処理技術の開発を目指すこととしている。

Outline of Final Research Achievements

The effect of the different conductivity of the Petri dishes used for fixing samples for PBII treatment on the sterilizing effect was investigated. The results showed that the sterilization effect was higher when conductive stainless steel was used at low applied voltages. On the other hand, at higher applied voltages, the dielectric breakdown occurred even when dielectric glass was used, resulting in no difference in sterilizing effect. Based on these findings and the results of rudimentary simulations, it was confirmed that the use of HHO gas and the stainless steel petri dish, even at a low applied voltage such as -2kV, showed a better sterilizing effect than nitrogen gas or oxygen gas. The PBII treatment was also applied to food materials to show that surface processing is possible.

Academic Significance and Societal Importance of the Research Achievements

本研究成果の学術的意義は、HHOガスをチャンバー内に導入し、サンプル固定用シャーレとしてステンレスを用いることで、酸素ガスや窒素ガスを用いるよりも印加電圧等を下げた状態、すなわち温和な条件でも効果的に殺菌出来る事を見いだしたことにある。また、サンプル固定用シャーレに誘電体であるガラスを用いたとしても、一定レベル以上の印加電圧を負荷すれば絶縁破壊が起こり、PBII処理が可能であることを見いだした。この結果は、誘電体である食品素材を対象とする場合でも、条件を整える事で、表面加工や殺菌処理が可能であり、PBII処理が食品加工に利用出来る可能性を示すものである。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (7 results)

All 2022 2020 2019

All Journal Article (3 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (4 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Effect of the conductivity of the petri dish placed on the electrode on the surface layer of the spores in the PBII method2022

    • Author(s)
      Kakugawa, K., Umemoto, S., Noda, T., Nosaki, K., Tanaka, T., Vutova, K.
    • Journal Title

      ELECTROTECHNICA & ELECTRONICA

      Volume: 57 Pages: 41-45

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The sterilization effect of plasma-based ion implantation on prokaryotic microorganisms2022

    • Author(s)
      Umemoto, S., Kakugawa, K., Nosaki, K. , Noda, T., Tanaka, T., Vutova, K.
    • Journal Title

      ELECTROTECHNICA & ELECTRONICA

      Volume: 57 Pages: 46-50

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 新規食品殺菌装置の開発を目的としたプラズマベースイオン注入(PBII)法のフラットサワー菌に対する殺菌効果2020

    • Author(s)
      角川幸治、田中武
    • Journal Title

      アグリバイオ

      Volume: 4(5) Pages: 72-76

    • NAID

      40022309366

    • Related Report
      2020 Research-status Report
  • [Presentation] Effect of the conductivity of the petri dish placed on the electrode on the surface layer of the spores in the PBII method2022

    • Author(s)
      Kakugawa, K., Umemoto, S., Noda, T., Nosaki, K., Tanaka, T., Vutova, K.
    • Organizer
      The Fourteen International conference on Electron Beam Technologies EBT 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The sterilization effect of plasma-based ion implantation on prokaryotic microorganisms2022

    • Author(s)
      Umemoto, S., Kakugawa, K., Nosaki, K. , Noda, T., Tanaka, T., Vutova, K.
    • Organizer
      The Fourteen International conference on Electron Beam Technologies EBT 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 酸素ガスを用いた、プラズマベースイオン注入法がGeobacillus stearothermophius の芽胞に与える影響について2020

    • Author(s)
      梅本章矢、酒瀬川健人、日山元子、田中武、角川幸治
    • Organizer
      日本農芸化学会中四国支部第58回講演会
    • Related Report
      2020 Research-status Report
  • [Presentation] Influence of ion sheath size on the sterilization effect of Geobacillus stearothermophilus by plasma-based ion implantation (PBII) method2019

    • Author(s)
      Koji Kakugawa, Motoko Hiyama, Yoshinobu Tsuchiya, Takeshi Tanaka
    • Organizer
      2019 international Conference on Agricultural and Food Science 3rd ICAFS
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research

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Published: 2019-04-18   Modified: 2024-01-30  

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