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Genetical and eco-physiological study on the ability of grain protein accumulation in rice

Research Project

Project/Area Number 19K06004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39020:Crop production science-related
Research InstitutionIshikawa Prefectural University

Principal Investigator

Tsukaguchi TadashiTadashi  石川県立大学, 生物資源環境学部, 准教授 (40345492)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsイネ / 子実 / 窒素 / 分配 / QTL / 子実窒素集積性 / 窒素分配 / 窒素転流 / 環境負荷 / 多収 / 量的形質遺伝子座 / 窒素利用効率 / タンパク質含量
Outline of Research at the Start

イネの多収と環境負荷の軽減の両立のためには、作物の窒素利用効率を高めることが不可欠である。高子実タンパク質含量が高品質の条件ではないイネでは、子実タンパク質濃度の低下による子実への窒素分配の抑制が窒素利用効率向上に寄与することが期待される。本研究は、登熟期のイネ植物体内の窒素分配を支配する要因の1つとして、ポテンシャルな子実窒素蓄積量を反映する子実窒素集積性に着目し、子実窒素集積性を遺伝学的および生理・生態学的に解明しようとするものである。

Outline of Final Research Achievements

Nitrogen accumulation in grain is associated with N partitioning in the grain-filling period in rice plants, affecting yield and N use efficiency because it accompanies N remobilization and translocation from vegetative parts. We found a close relation between N content in total plants per grain dry weight (TNA/GW) and grain nitrogen concentration (GNC) and a large cultivar difference in the regression coefficient (A) between them. Using the population derived from Momiroman, with low A, and Takanari, with high A, we found quantitative trait loci (QTLs) for A on chromosomes 6 and 10, the Takanari alleles of both QTLs increased the value. We produced near isogenic lines (NILs) to validate the effect of the QTLs. NILs with the Takanari allele in each region had higher A than NILs with the Momiroman alleles, verifying the effect of the QTLs on A.

Academic Significance and Societal Importance of the Research Achievements

イネなどの作物において子実への窒素分配は収量性や窒素利用効率に強く影響する。イネにおいて窒素分配に関わると考えられる子実の窒素集積性が遺伝形質であることを明らかにし、その定量評価法の確立およびその評価法による量的形質遺伝子座(QTL)の推定を行ってきた。本課題においてこのQTLの子実窒素集積性への効果が実証され、子実への窒素分配を通じた収量性や窒素利用効率の向上につながることが期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (17 results)

All 2022 2021 2020 2019

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 9 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Estimation of spikelet number per area by UAV-acquired vegetation index in rice (Oryza sativa L.)2022

    • Author(s)
      Tadashi Tsukaguchi, Haruka Kobayashi, Yoichi Fujihara, and Shunsuke Chono
    • Journal Title

      Plant Production Science

      Volume: 25 Issue: 1 Pages: 20-29

    • DOI

      10.1080/1343943x.2021.1943467

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 空撮画像に基づいた水稲の倒伏程度と出穂20~10日前の植生指数との関係2022

    • Author(s)
      元川開斗, 藤原洋一, 塚口直史
    • Journal Title

      北陸作物学会報

      Volume: 57 Pages: 29-32

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of Seeding Density per Seedling Box and Planting Density on Production Cost of High-yielding Rice Varieties in Ishikawa Prefecture2021

    • Author(s)
      宇野史生, 島田雅博, 中村弘和, 吉田翔伍, 塚口直史
    • Journal Title

      Japanese Journal of Crop Science

      Volume: 90 Issue: 3 Pages: 252-260

    • DOI

      10.1626/jcs.90.252

    • NAID

      130008070981

    • ISSN
      0011-1848, 1349-0990
    • Year and Date
      2021-07-05
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of Soybean Seed Yield Using Vegetation Index Captured with UAV2021

    • Author(s)
      丹保 彩香, 島田 雅博, 吉藤 昭紀, 今本 裕士, 永畠 秀樹, 藤原 洋一, 塚口 直史
    • Journal Title

      Japanese Journal of Crop Science

      Volume: 90 Issue: 3 Pages: 261-268

    • DOI

      10.1626/jcs.90.261

    • NAID

      130008070978

    • ISSN
      0011-1848, 1349-0990
    • Year and Date
      2021-07-05
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 空撮によって得られた生殖成長期の植生指数によるダイズ子実収量の解析2021

    • Author(s)
      丹保彩香・島田雅博・吉藤昭紀・今本裕士・永畠秀樹・藤原洋一・塚口直史
    • Journal Title

      日本作物学会紀事

      Volume: 90

    • NAID

      130008070978

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 石川県における水稲多収品種の生産費が最小になる育苗箱当たり播種量および栽植密度2021

    • Author(s)
      宇野史生・島田雅博・中村弘和・吉田翔伍・塚口直史
    • Journal Title

      日本作物学会紀事

      Volume: 90

    • NAID

      130008070981

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 水稲の登熟期の掛流し灌漑が水田の窒素環境に与える影響2020

    • Author(s)
      西田和弘・岩崎里子・塚口直史・吉田修一郎
    • Journal Title

      農業農村工学会誌

      Volume: 88 Pages: 15-18

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of Continuous Irrigation with Running Water Using Cool and Low Nitrogen Concentration Irrigation Water during Rice Grain Filling Period on Grain Protein Content and Percentages of Chalky Grains of Rice2019

    • Author(s)
      西田和弘・塚口直史・柴田里子・吉田修一郎・塩沢昌
    • Journal Title

      Transactions of The Japanese Society of Irrigation, Drainage and Rural Engineering

      Volume: 87 Issue: 2 Pages: I_219-I_226

    • DOI

      10.11408/jsidre.87.I_219

    • NAID

      130007723643

    • ISSN
      1882-2789, 1884-7242
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Diagnosis of Rice Growth Using a Multispectral Camera: Evaluation of Nitrogen Absorption2019

    • Author(s)
      藤原洋一・塚口直史・長野峻介
    • Journal Title

      Transactions of The Japanese Society of Irrigation, Drainage and Rural Engineering

      Volume: 87 Issue: 2 Pages: IV_7-IV_8

    • DOI

      10.11408/jsidre.87.IV_7

    • NAID

      130007756639

    • ISSN
      1882-2789, 1884-7242
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Estimation of the Heading Dates of Rice Cultivar 'Ishikawa65gou' by Using a Phenological development Model2019

    • Author(s)
      宇野史生・島田雅博・吉田ひろえ・中川博視・塚口直史
    • Journal Title

      The Hokuriku Crop Science

      Volume: 54 Issue: 0 Pages: 38-40

    • DOI

      10.19016/hokurikucs.54.0_38

    • NAID

      130007657741

    • ISSN
      0388-8061, 2189-7417
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Detection of Lodging Area in a Paddy Field from a Digital Surface Model (DSM)2021

    • Author(s)
      Tadashi Tsukaguchi, Fumio Uno, Yoichi Fujihara
    • Organizer
      The 10th Asian Crop Science Association Conference
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ドローン空撮に基づいた水稲の倒伏評価2021

    • Author(s)
      元川開斗, 藤原洋一, 塚口直史
    • Organizer
      第57回北陸作物・育種学会講演会
    • Related Report
      2021 Annual Research Report
  • [Presentation] UAVによる植生指数と水稲窒素吸収量の関係2019

    • Author(s)
      澤邊穂乃佳・小林春香・藤原洋一・長野峻介・塚口直史
    • Organizer
      日本作物学会第248回講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] 植生指数を用いたダイズ品質に関わる生育評価2019

    • Author(s)
      丹保彩香・三納礼奈・今本裕士・永畠秀樹・塚口直史
    • Organizer
      日本作物学会第248回講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] UAVによる植生指数と植被率およびSPAD値の関係2019

    • Author(s)
      小林春香・土山優衣・澤邉穂乃佳・塚口直史
    • Organizer
      日本作物学会第248回講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] 灌漑水の窒素濃度が冷水掛流し灌漑水田の 玄米タンパク質濃度および白未熟粒割合に与える影響2019

    • Author(s)
      西田和弘・松本悠志・塚口直史・吉田修一郎
    • Organizer
      令和元年度農業農村工学会大会講演会
    • Related Report
      2019 Research-status Report
  • [Presentation] スペクトルカメラを搭載した UAV による水稲診断法の確立2019

    • Author(s)
      丸山拓巳・藤原洋一・塚口直史・長野峻介・一恩英二
    • Organizer
      日本生態学会中部地区大会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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