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Mechanism of ascorbic acid and amino acids high-accumulation in introgression lines of tomato

Research Project

Project/Area Number 19K06012
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionTohoku University

Principal Investigator

Kazuhisa Kato  東北大学, 農学研究科, 准教授 (30613517)

Co-Investigator(Kenkyū-buntansha) 金山 喜則  東北大学, 農学研究科, 教授 (10233868)
池田 裕樹  宇都宮大学, 農学部, 助教 (90782053)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsトマト / ビタミンC / アミノ酸 / 近縁野生種 / 染色体断片置換系統 / アスコルビン酸 / 高蓄積機構
Outline of Research at the Start

トマトは日常的に消費するため、国民の健全な生活に貢献しうる野菜であるが、栽培化の過程で有用成分の低下が生じている。そこで本研究では、抗酸化能ほか多面的な機能性が医学的に証明されているビタミンCと、トマトのうま味の主成分であるとともに健康機能性も有するアミノ酸を、近縁野生種の遺伝的多様性のポテンシャルを活かして高蓄積するトマトの開発を行う。

Outline of Final Research Achievements

Useful components are reduced in tomato compared to wild tomato. In this study, introgression lines of tomato, in which part of the chromosome of a cultivated species was replaced with the chromosome of a wild species was used to understand the mechanism in high accumulation of useful components. L-ascorbic acid (L-AsA) is an abundant antioxidant in plants and plays an important role in detoxifying reactive oxygen species (ROS) produced during stress, and amino acids are the main components of tomato's umami and have health functionality. One IL line with high accumulation was screened, respectively. In the ascorbic acid high-accumulation line, comprehensive expression analysis of biosynthetic genes was performed to clarify a part of the ascorbic acid high-accumulation mechanism. Regarding the high amino acid accumulation line, the relationship between various amino acid contents and the expression of biosynthetic genes was investigated.

Academic Significance and Societal Importance of the Research Achievements

トマトは栽培品種化の過程で有用成分の低下が生じている。本研究成果より、ビタミンCおよびアミノ酸といった有用成分を高蓄積するメカニズムを明らかにすることで、交配により、機能性の高いトマトの育種可能となる。ビタミンCはトマトの含有率は高くないものの、消費量が最も多い野菜の一つであることから、日常的に多く食する。そのため、アスコルビン酸を多く含むトマトを作出すれば、健康増進への寄与が著しく高まり、園芸学の現代的課題の1つでもある医療費抑制への貢献が期待される。また、アミノ酸含量が高いトマトを作出すれば、味の向上とともに人の健康増進へも貢献できる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (55 results)

All 2022 2021 2020 2019

All Journal Article (17 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 15 results,  Open Access: 7 results) Presentation (36 results) (of which Int'l Joint Research: 9 results,  Invited: 4 results) Book (2 results)

  • [Journal Article] Effect of short-term temperature stress on fruit set and the expression of an auxin reporter gene and auxin synthesis genes in tomato2022

    • Author(s)
      Ito Hikaru、Kanayama Yoshinori、Shibuya Tomoki、Mohammed Seedahmed A.、Nishiyama Manabu、Kato Kazuhisa
    • Journal Title

      Scientia Horticulturae

      Volume: 300 Pages: 111039-111039

    • DOI

      10.1016/j.scienta.2022.111039

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effects of light quality on plant development and fruit metabolism and their regulation by plant growth regulators in tomato2022

    • Author(s)
      Xiao Lingran、Shibuya Tomoki、Kato Kazuhisa、Nishiyama Manabu、Kanayama Yoshinori
    • Journal Title

      Scientia Horticulturae

      Volume: 300 Pages: 111076-111076

    • DOI

      10.1016/j.scienta.2022.111076

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Physiological Investigation of Quantitative Traits for Fruit Mass Assessment Using a Tomato Introgression Line2022

    • Author(s)
      Yada Haruna、Matsumoto Chihiro、Xie Xiaonan、Kato Kazuhisa、Ikeda Hiroki
    • Journal Title

      The Horticulture Journal

      Volume: 91 Issue: 2 Pages: 221-228

    • DOI

      10.2503/hortj.UTD-356

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular breeding of miraculin-accumulating tomatoes with suitable traits for cultivation in plant factories with artificial lightings and the optimization of cultivation methods2022

    • Author(s)
      Hiwasa-Tanase Kyoko、Kato Kazuhisa、Ezura Hiroshi
    • Journal Title

      Plant Factory Basics, Applications and Advances

      Volume: - Pages: 377-390

    • DOI

      10.1016/b978-0-323-85152-7.00010-0

    • ISBN
      9780323851527
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Post-Heat Treatment on the Flavor and Nutritional Components of Tomato Puree Concentrated by a Vacuum Microwave2021

    • Author(s)
      Sasaki Kotomi、Orikasa Takahiro、Kato Kazuhisa、Matsushima Uzuki、Koide Shoji
    • Journal Title

      Nippon Shokuhin Kagaku Kogaku Kaishi

      Volume: 68 Issue: 3 Pages: 115-123

    • DOI

      10.3136/nskkk.68.115

    • NAID

      130008002712

    • ISSN
      1341-027X, 1881-6681
    • Year and Date
      2021-03-15
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 減圧マイクロ波によるトピューレの濃縮 -前加熱処理が食味と成分に与える影響 -2021

    • Author(s)
      佐々木琴瑞, 折笠貴寛, 加藤一幾, 松嶋卯月, 小出章二
    • Journal Title

      農業食料工学会東北支部報

      Volume: 68 Pages: 19-24

    • Related Report
      2021 Annual Research Report
  • [Journal Article] ウォールフラワー4品種および黄色系ストック15品種の花色と 花色素に関する研究2021

    • Author(s)
      小林公美 , 中條しづ子 , 加藤一幾 , 竹原明秀 , 三浦 靖 , 立澤文見
    • Journal Title

      岩手大学人文社会科学部紀要

      Volume: 109 Pages: 125-136

    • NAID

      40022825078

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Flavonoids in the flowers of Primula ×polyantha Mill. and Primula primulina (Spreng.) H. Hara (Primulaceae)2021

    • Author(s)
      Tatsuzawa Fumi、Mizuno Takayuki、Kikuchi Ryo、Kato Kazuhisa、Ota Toru、Murai Yoshinori、Yangzom Rinchen、Iwashina Tsukasa
    • Journal Title

      Phytochemistry

      Volume: 189 Pages: 112827-112827

    • DOI

      10.1016/j.phytochem.2021.112827

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Physiological Characterization of Tomato Introgression Line IL5-4 That Increases Brix and Blossom-end Rot in Ripening Fruit2021

    • Author(s)
      Matsumoto Chihiro、Yada Haruna、Hayakawa Chie、Hoshino Koichi、Hirai Hideaki、Kato Kazuhisa、Ikeda Hiroki
    • Journal Title

      The Horticulture Journal

      Volume: 90 Issue: 2 Pages: 215-222

    • DOI

      10.2503/hortj.UTD-264

    • NAID

      130008028818

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 果菜類の育苗において低濃度オゾン水が生育に及ぼす影響2021

    • Author(s)
      菅原愼太郎 , 田村香織 , 立澤文見 , 金山喜則 , 加藤一幾
    • Journal Title

      医療・環境オゾン研究

      Volume: 28 Pages: 34-42

    • NAID

      40022548932

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of the FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 Homolog SlFKF1 in Tomato as a Model for Plants with Fleshy Fruit2021

    • Author(s)
      Shibuya Tomoki、Nishiyama Manabu、Kato Kazuhisa、Kanayama Yoshinori
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 4 Pages: 1735-1735

    • DOI

      10.3390/ijms22041735

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Physiological roles of tryptophan decarboxylase revealed by overexpression of SlTDC1 in tomato2021

    • Author(s)
      Tsunoda Yui、Hano Shohei、Imoto Nozomi、Shibuya Tomoki、Ikeda Hiroki、Amagaya Kayoko、Kato Kazuhisa、Shirakawa Hitoshi、Aso Hisashi、Kanayama Yoshinori
    • Journal Title

      Scientia Horticulturae

      Volume: 275 Pages: 109672-109672

    • DOI

      10.1016/j.scienta.2020.109672

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of PcLEA14, a Group 5 Late Embryogenesis Abundant Protein Gene from Pear (Pyrus communis)2020

    • Author(s)
      Shibuya Tomoki、Itai Ryota、Maeda Minori、Kitashiba Hiroyasu、Isuzugawa Kanji、Kato Kazuhisa、Kanayama Yoshinori
    • Journal Title

      Plants

      Volume: 9 Issue: 9 Pages: 1138-1138

    • DOI

      10.3390/plants9091138

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of FLOWERING LOCUS C Homologs in Apple as a Model for Fruit Trees2020

    • Author(s)
      Kagaya Hidenao、Ito Naoko、Shibuya Tomoki、Komori Sadao、Kato Kazuhisa、Kanayama Yoshinori
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 12 Pages: 4562-4562

    • DOI

      10.3390/ijms21124562

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Interannual variation in the harvest time of mini cauliflower in the Sanriku coastal region2020

    • Author(s)
      OKADA Masumi、MATSUSHIMA Uzuki、KATO Kazuhisa
    • Journal Title

      Climate in Biosphere

      Volume: 20 Issue: 0 Pages: 55-58

    • DOI

      10.2480/cib.J-20-059

    • NAID

      130007830291

    • ISSN
      1346-5368, 2185-7954
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Confirmation of Everbearing Mutants in the Everbearing Strawberry Cultivar ‘Natsuakari’ and the Effect of an Overwintering Condition on Inflorescence and Runner Development2020

    • Author(s)
      Hamano Megumi、Honjo Masanori、Sato Shiori、Ito Atsushi、Machita So、Matsushima Uzuki、Okada Masumi、Kato Kazuhisa
    • Journal Title

      The Horticulture Journal

      Volume: 89 Issue: 2 Pages: 167-174

    • DOI

      10.2503/hortj.UTD-133

    • NAID

      130007828976

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization and Expression Analysis of the Ca2+/Cation Antiporter Gene Family in Tomatoes2019

    • Author(s)
      Amagaya Kayoko、Shibuya Tomoki、Nishiyama Manabu、Kato Kazuhisa、Kanayama Yoshinori
    • Journal Title

      Plants

      Volume: 9:25 Issue: 1 Pages: 25-25

    • DOI

      10.3390/plants9010025

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Malus domestica とMalus hupehensis におけるTEMPRANILLO ホモログと幼若性の関係2022

    • Author(s)
      伊藤尚子, 小森貞男, 加藤一幾, 金山喜則
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] デルフィニウムの花成に及ぼす光質の影響とフィトクロム遺伝子に関する研究2022

    • Author(s)
      沼澤芽生, 菅原のの, 阿部遼, 西山学, 加藤一幾, 金山喜則
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 低濃度オゾン水処理がトマト台木の挿し芽発根性と病害虫抵抗性関連遺伝子の発現に及ぼす影響2022

    • Author(s)
      高橋菜央子, 西山学, 金山喜則, 加藤一幾
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] トマト染色体断片置換系統IL12-1-1 の果実サイズに関わる植物ホルモンと遺伝子の解析2022

    • Author(s)
      谷田春菜, 謝肖男, 加藤一幾, 池田裕樹
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] パプリカの栽培および貯蔵における温度が果実品質に及ぼす影響2022

    • Author(s)
      青代香菜子, 西山学, 金山喜則, 加藤一幾
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Role of FLAVIN-BINDING, KELCH REPEAT and F-BOX 1 Homolog SlFKF1 in Flowering Initiation and Carotenoid Accumulation in Tomato2021

    • Author(s)
      Tomoki Shibuya, Manabu Nishiyama, Kazuhisa Kato, Yoshinori Kanayama
    • Organizer
      JSOL2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effects of low-concentration ozonated water on the growth and biotic stress tolerance in tomato rooted cuttings2021

    • Author(s)
      Naoko Takahashi, Manabu Nishiyama, Yoshinori Kanayama, Kazuhisa Kato
    • Organizer
      JSOL2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A long-day treatment by red LED promotes flowering in Delphinium2021

    • Author(s)
      Nono Sugawara, Mei Numazawa, Manabu Nishiyama, Kazuhisa Kato, Yoshinori Kanayama
    • Organizer
      JSOL2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Integrated analyses of the metabolome and transcriptome to reveal the effect of light quality on metabolism in tomato fruit2021

    • Author(s)
      Lingran Xiao, Tomoki Shibuya, Manabu Nishiyama, Kazuhisa Kato, Yoshinori Kanayama
    • Organizer
      JSOL2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Physiological mechanisms of fruit mass increase in tomato introgression line IL12-1-12021

    • Author(s)
      Haruna Yada, Chihiro Matsumoto, Xiaonan Xie, Kazuhisa Kato, Hiroki Ikeda
    • Organizer
      JSOL2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] プラズマ照射溶液がレタスの生育および抗酸化能に及ぼす影響;次世代蓄電池を用いたプラズマ農業の実用化に向けた研究;2021

    • Author(s)
      肖 凌冉, 高島圭介, 金子俊郎, 千葉一美, 西山 学, 高橋英樹, 金山喜則, 加藤一幾
    • Organizer
      SMILEcoみやぎシンポジウム
    • Related Report
      2021 Annual Research Report
  • [Presentation] 光質がトマト果実の遺伝子発現プロファイルに及ぼす影響2021

    • Author(s)
      肖凌冉, 渋谷知暉, 西山学, 加藤一幾, 金山喜則
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Malus domestica と Malus hupehensis における FLOWERING LOCUS C ホモログと幼若性の関係2021

    • Author(s)
      伊藤尚子, 加賀谷秀直, 小森貞男, 加藤一幾, 金山喜則
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] トマト果実における GCaMP6 を用いたカルシウ ムイメージング2021

    • Author(s)
      堀千秋, 栗原大輔, 大村道明, 西山学, 金山喜則, 加藤一幾
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] エラータム系およびシネンシス系デルフィニウムの花成と切り花品質に及ぼす光質の影響2021

    • Author(s)
      阿部遼, 沼澤芽生, 西山学, 加藤一幾, 金山喜則
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] トマトの染色体断片置換系 統 IL5-4 における果実の高 糖度化および尻腐れ発生要 因の解明2021

    • Author(s)
      松本千紘, 谷田春菜, 早川智恵, 星野幸一, 平井英明, 加藤一幾, 池田裕樹
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 栽培および貯蔵温度がカラーピーマンの収量や品質に及ぼす影響2021

    • Author(s)
      青代香菜子, 西山学, 加藤一幾, 金山喜則
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] リコペン高蓄積を示すトマト染色体断片置換系統の各種カロテノイド分析2021

    • Author(s)
      神田駿哉, 西山学, 金山喜則, 加藤一幾
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 低濃度オゾン水がトマト台木の挿し木発根性に及ぼす影響2021

    • Author(s)
      高橋菜央子, 田村香理, 西山学, 金山喜則, 加藤一幾
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] 公開 葛尾村植物工場におけるマンゴーおよび有機トマト生産による事業化の検討2020

    • Author(s)
      加藤一幾, 坪井真喜, 岩本正敏, 菅野均志, 西山 学, 大磯 真美, 牧野 知之, 北柴 大泰, 金山 喜則, 小倉 振一郎
    • Organizer
      東北大学農学研究科 東北復興農学センター主催シンポジウム 震災から10年: 「復興農学」の成果と課題
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] GCaMP6 を用いたカルシウムイメージングのトマトへの応用2020

    • Author(s)
      堀千秋,伊藤輝,栗原大輔,石田宏幸,大村道明,金山喜則,加藤一幾
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] トマトの染色体断片置換系統におけるアミノ酸高蓄積系統の各種アミノ酸分析2020

    • Author(s)
      西澤啓,西山学,大村道明,金山喜則,加藤一幾
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] リコペン高蓄積を示すトマト染色体断片置換系統の解析2020

    • Author(s)
      神田駿哉,西山学,金山喜則,加藤一幾
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] Effect of light quality on metabolic profile in tomato2020

    • Author(s)
      肖凌冉,渋谷知暉,西山学,加藤一幾,金山喜則
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] トマト IL8-3 領域の E3 ユビキチンリガーゼ様遺伝子の RNAi による発現抑制2020

    • Author(s)
      薗部彰,渋谷知暉,西山学,加藤一幾,金山喜則
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] トマトの染色体断片置換系統を用いた L- アスコルビン酸高蓄積機構の解明2020

    • Author(s)
      武田彩季,西山学,大村道明,金山喜則,加藤一幾
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 低濃度オゾン水の野菜栽培への応用利用2019

    • Author(s)
      加藤一幾
    • Organizer
      日本機能水学会第18回学術大会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Fruit set and auxin distribution in tomato.2019

    • Author(s)
      Kato, K,Ito, H,Shibuya, T,Kanayama, Y
    • Organizer
      The 16th Japan Solanaceae Consortium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Mechanism of high L-ascorbic acid accumulation in an introgression line of tomato2019

    • Author(s)
      Takeda, S,Nishiyama, M,Omura, M,Kanayama, Y,Kato, K
    • Organizer
      The 16th Japan Solanaceae Consortium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Study on genes related to high sugar content derived from chromosome fragments of wild relatives of tomato2019

    • Author(s)
      Sonobe, A,Shibuya, T,Kato, K,Kanayama, Y
    • Organizer
      The 16th Japan Solanaceae Consortium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mechanism of the amino acids accumulation in an introgression line of tomato.2019

    • Author(s)
      Nishizawa, H,Oomura, M,Kanayama, Y,Kato, K
    • Organizer
      The 16th Japan Solanaceae Consortium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] トマトの染色体断片置換系統における果実サイズと糖度の系統間比較2019

    • Author(s)
      松本千紘,加藤一幾,池田裕樹
    • Organizer
      園芸学会令和元年度秋季大会
    • Related Report
      2019 Research-status Report
  • [Presentation] トマトにおけるGCaMP6を用いたカルシウムイメージングの検討2019

    • Author(s)
      堀 千秋,伊藤 輝,栗原大輔,大村道明,金山喜則,加藤一幾
    • Organizer
      園芸学会東北支部令和元年度大会
    • Related Report
      2019 Research-status Report
  • [Presentation] トマト果実の代謝物濃度に及ぼす光質の影響2019

    • Author(s)
      肖 凌冉,渋谷知暉,西山 学,加藤一幾,金山喜則
    • Organizer
      園芸学会東北支部令和元年度大会
    • Related Report
      2019 Research-status Report
  • [Presentation] トマトの染色体断片置換系統におけるリコペン高蓄積系統の選抜2019

    • Author(s)
      神田駿哉,西山学,金山喜則,加藤一幾
    • Organizer
      園芸学会東北支部令和元年度大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 低濃度オゾン水の園芸作物栽培における有効活用2019

    • Author(s)
      加藤一幾,立澤文見,金山喜則
    • Organizer
      日本オゾン協会第28回年次研究講演会
    • Related Report
      2019 Research-status Report
  • [Book] 地力アップ大事典2022

    • Author(s)
      加藤一幾(437-444)
    • Total Pages
      960
    • Publisher
      農山漁村文化協会
    • ISBN
      9784540212147
    • Related Report
      2021 Annual Research Report
  • [Book] 野菜園芸学 第2版2020

    • Author(s)
      金山 喜則
    • Total Pages
      326
    • Publisher
      文永堂出版
    • ISBN
      9784830041372
    • Related Report
      2020 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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