• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Do herbivorous caterpillars suppress plant defense responses with the enzyme from their silk glands?

Research Project

Project/Area Number 19K06070
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39050:Insect science-related
Research InstitutionKyoto University

Principal Investigator

Ozawa Rika  京都大学, 生態学研究センター, 研究員 (90597725)

Co-Investigator(Kenkyū-buntansha) 塩尻 かおり  龍谷大学, 農学部, 教授 (10591208)
松井 健二  山口大学, 大学院創成科学研究科, 教授 (90199729)
Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsみどりの香り(GLVs) / チョウ目幼虫 / 直接防衛 / 間接防衛 / 絹糸腺 / 寄生蜂 / FHD / 防衛回避 / 被食防衛 / みどりの香り(GLV) / 関節防衛 / みどりの香り / チョウ目昆虫
Outline of Research at the Start

植物の誘導的な被食防衛に対し、植食者がそれを積極的に抑制する現象に近年注目が集まっているが、その実態にはいまだ不明な点が多い。本研究では、チョウ目昆虫に広く分布する、植物の「みどりの香り」の生合成を抑制する酵素に注目し、「みどりの香り抑制酵素がチョウ目幼虫による食草の防衛回避に寄与している」という仮説を立てこれを検証する。具体的にはチョウ目幼虫について、本酵素の①酵素としての存在と機能、②食草の誘導的な被食防衛に対する抑制作用を明らかにすることを試みる。本研究成果は、チョウ目昆虫の植食性獲得進化に新しい知見をもたらすとともに、農業害虫としてのチョウ目幼虫の管理につながる基盤的研究である。

Outline of Final Research Achievements

We have already identified the enzyme, fatty acid hydroperoxide dehydratase (BmFHD), in the silk glands of the silkworm that inhibits the production of green leaf volatiles. We found that the enzyme plays a role in the avoidance of the indirect defense of mulberry plants by silkworms. We also found similar sequences of the BmFHD gene in other lepidopteran species. Based on these findings, we proposed that FHD in caterpillars may act to suppress plant defense responses in general. To test this hypothesis, we generated FHD-knockout (FHD-KO) armyworms and studied the effects of armyworm’s FHD on the avoidance of direct and indirect defenses in maize plants. We compared the growth of FHD-KO and wild-type armyworms on maize plants. Further, we examined the response of parasitoid wasps to maize plants infested by either FHD-KO or wild-type armyworms. The results showed that FHD was involved in the avoidance of direct defense mechanisms of maize plants against armyworms.

Academic Significance and Societal Importance of the Research Achievements

多くの植物が放出するみどりの香りの機能について、これまで知られていなかったチョウ目幼虫に対する食害抵抗性に関わることを明らかにした。また、チョウ目昆虫であるアワヨトウがもつ酵素(FHD)が、みどりの香りによる植物の抵抗性を回避する機能を持つことを示した。チョウ目幼虫の多くは食葉性であり、葉菜などの農作物に対する害虫となっている。今回明らかにしたみどりの香りの成長抑制活性を利用することで安心安全な害虫管理への応用が期待できる。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (13 results)

All 2024 2023 2022 2021 2020

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results) Presentation (9 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Uninfested eggplants exposed to volatiles from conspecific plants infested by omnivorous mirids exhibit jasmonic acid priming and enhanced defenses against herbivores2024

    • Author(s)
      Hojun Rim、Masayoshi Uefune、Rika Ozawa、Junji Takabayashi
    • Journal Title

      Arthropod-Plant Interactions

      Volume: 18 Issue: 2 Pages: 205-210

    • DOI

      10.1007/s11829-023-10010-z

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Olfactory responses of Nesidiocoris tenuis to uninfested or conspecific-infested banker plants and Thrips palmi-infested eggplants2024

    • Author(s)
      Yoneya Kinuyo、Nishimori Satsuki、Yano Eizi、Yamaguchi Kei、Tsumoto Matsuri、Ozawa Rika、Takabayashi Junji、Kandori Ikuo
    • Journal Title

      BioControl

      Volume: 69 Issue: 1 Pages: 19-28

    • DOI

      10.1007/s10526-023-10234-1

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fecundity of diamondback moth females when offered honey with pyridalyl, a selective insecticide2023

    • Author(s)
      Rika Ozawa、Masayoshi Uefune、Junji Takabayashi
    • Journal Title

      Journal of Applied Entomology

      Volume: 148 Issue: 1 Pages: 86-92

    • DOI

      10.1111/jen.13209

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Booklice <i>Liposcelis bostrychophila</i> are efficiently attracted by the combination of 2,3,5,6‐tetramethylpyrazine and ultraviolet light2023

    • Author(s)
      Kohjiro Tanaka、Rika Ozawa、Junji Takabayashi、Masanori Ochiai、Yoichi Hayakawa
    • Journal Title

      Pest Management Science

      Volume: 80 Issue: 2 Pages: 426-432

    • DOI

      10.1002/ps.7773

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Presentation] 植物のみどりの香りを用いたチョウ目幼虫に対する防衛機能2024

    • Author(s)
      小澤理香、塩尻かおり、大田航、大野裕香、藤田涼平、中尾拓磨、白井雄、大門高明、伊達みのり、松井健二、高林純示
    • Organizer
      日本昆虫学会第84回大会 第68回日本応用動物昆虫学会大会 合同大会(仙台)
    • Related Report
      2023 Annual Research Report
  • [Presentation] Functions of Green Leaf Volatiles in Direct and Indirect Defense of Plants against Herbivores2023

    • Author(s)
      Rika Ozawa, Ryohei Fujita, Takuma Nakao, Yuka Ohno, Wataru Ota, Kaori Shiojiri, Kyutaro Kishimoto, Kenji Matsui, Junji Takabayashi
    • Organizer
      the 33rd International Conference on Arabidopsis Research (ICAR2023)(Chiba)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Direct and Indirect Defenses against Insects in Plants Associated with Green Leaf Volatiles2023

    • Author(s)
      Rika Ozawa, Ryohei Fujita, Takuma Nakao, Yuka Ohno, Wataru Ota, Kaori Shiojiri, Kyutaro Kishimoto, Kenji Matsui, Junji Takabayashi
    • Organizer
      CNRS International Research Network France-Japan Frontiers in Plant Biology 2023 Kyoto Symposium (Kyoto)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] チョウ目幼虫の絹糸腺由来の酵素は植物の防衛機能を抑制しているか2023

    • Author(s)
      小澤理香、塩尻かおり、大田航、大野裕香、藤田涼平、中尾拓磨、白井雄、大門高明、田中萌菜、松井健二、高林純示
    • Organizer
      第55回種生物学シンポジウム(岡崎)
    • Related Report
      2023 Annual Research Report
  • [Presentation] 殺虫剤の摂食がコナガの次世代数に与える影響2023

    • Author(s)
      小澤理香、上船雅義、高林純示
    • Organizer
      第67回 日本応用動物昆虫学会 (大阪)
    • Related Report
      2022 Research-status Report
  • [Presentation] Synergistic effects of uninfested plants and honey on the attractiveness of the synthetic host-infested plant volatiles to parasitoid wasps2022

    • Author(s)
      Rika Ozawa, Yoshitsugu Ohara, Kaori Shiojiri, Toru Uchida, Kazumasa Kakibuchi, Kota Sano, Masayoshi Uefune, Junji Takabayashi
    • Organizer
      XXVI International Congress of Entomology, Helsinki
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] アワヨトウ絹糸腺由来の酵素による植物の防衛機能の抑制2022

    • Author(s)
      小澤理香、塩尻かおり、大田航、大野裕香、白井雄、大門高明、田中萌菜、松井健二、高林純示
    • Organizer
      日本応用動物昆虫学会 第66回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 植物のみどりの香りがコナガ幼虫の成長を抑制する2021

    • Author(s)
      小澤理香、塩尻かおり、中尾拓磨、藤田涼平、松井健二、高林純示
    • Organizer
      第65回 日本応用動物昆虫学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] みどりの香りの生合成抑制とその生態学的意義2020

    • Author(s)
      大西真由、後藤智哉、塩尻かおり、小澤理香
    • Organizer
      第31回新春技術講演会
    • Related Report
      2019 Research-status Report

URL: 

Published: 2019-04-18   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi