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Analysis of genes for salt-tolerance in green foxtail toward breeding of salt-tolerant foxtail millet

Research Project

Project/Area Number 19K06341
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 41050:Environmental agriculture-related
Research InstitutionKyoto Prefectural University

Principal Investigator

Ohsako Takanori  京都府立大学, 生命環境科学研究科, 准教授 (80363969)

Co-Investigator(Kenkyū-buntansha) 福永 健二  県立広島大学, 生命環境学部, 教授 (50435533)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsアワ / エノコログサ / 耐塩性 / QTL解析 / GRAS-Di / 適応進化 / ハマエノコロ / QTLマッピング / GWAS
Outline of Research at the Start

本研究では、(1)エノコログサ・ハマエノコロ系統を用いたゲノムワイド関連解析、(2)アワ-ハマエノコロ雑種由来の分離集団を用いたQTL解析の2種類の手法により、耐塩性関連遺伝子座の解明を目指す。耐塩性の低いアワと耐塩性の高いハマエノコロの雑種分離集団を用いたQTL解析により、ハマエノコロに耐塩性をもたらす遺伝子座の数と効果を明らかにする。次世代シーケンサーを利用したジェノタイピングにより高精度のマッピングを実現することを期待している。2つの分離集団それぞれについての遺伝子地図の比較により、ハマエノコロの各系統が持つ耐塩性遺伝子座の共通性と差異を明らかにする。

Outline of Final Research Achievements

We constructed linkage maps using two segregating populations derived from a cross between foxtail millet (74-12-6-3) and coastal green foxtail (Jogashima 1) and a cross between green foxtail (Katsurasaka 2) and coastal green foxtail (Mitsu 4) with GRAS-Di markers. Tolerance to salt stress was evaluated with F3 seedlings by hydroponic culture using media containing 100 mM and 0 mM (control) NaCl.
For 74-12-6-3 x Jogashima 1 population, a QTL for salt stress tolerance was detected on chromosome 5. For QTLs were seen on chromosomes 2, 3, 5, and 9 for Katsurasaka 2 x Mitsu 4 population. Our result indicates that geographically distinct populations possess different genetic architectures for salt stress tolerance and suggests parallel evolution of the traits for adaptation to coastal habitat.

Academic Significance and Societal Importance of the Research Achievements

2系統のハマエノコロの耐塩性の遺伝的基盤が異なることが示され、国内のハマエノコロの耐塩性が平行進化により独立に獲得されたことが示唆された。この成果は海浜環境における植物の適応進化の解明に寄与するものである。
異なる野生種由来の複数の耐塩性関連遺伝子を交配などにより導入することで強力な耐塩性を持つ新規アワ系統を育成できる可能性が示され、塩性化土壌における作物生産の拡大に貢献する成果を得た。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (4 results)

All 2022 2020

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (1 results)

  • [Journal Article] Recombinant inbred lines and next-generation sequencing enable rapid identification of candidate genes involved in morphological and agronomic traits in foxtail millet2022

    • Author(s)
      Fukunaga Kenji、Abe Akira、Mukainari Yohei、Komori Kaho、Tanaka Keisuke、Fujihara Akari、Yaegashi Hiroki、Kobayashi Michie、Ito Kazue、Ohsako Takanori、Kawase Makoto
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 218-218

    • DOI

      10.1038/s41598-021-04012-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Green foxtail, <i>Setaria viridis</i> (L.) P. Beauv. as a model plant—its application for weed biology2020

    • Author(s)
      Fukunaga Kenji、Ohsako Takanori
    • Journal Title

      Journal of Weed Science and Technology

      Volume: 65 Issue: 4 Pages: 140-149

    • DOI

      10.3719/weed.65.140

    • NAID

      130007980569

    • ISSN
      0372-798X, 1882-4757
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Classification and systematics of the <i>Fagopyrum</i> species2020

    • Author(s)
      Takanori Ohsako, Chengyun Li
    • Journal Title

      Breeding Science

      Volume: 70 Issue: 1 Pages: 93-100

    • DOI

      10.1270/jsbbs.19028

    • NAID

      130007809746

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] アワ近縁野生種ハマエノコロの耐塩性に関するQTL解析2020

    • Author(s)
      田中智子、山本菜穂子、福永健二、大迫敬義
    • Organizer
      日本育種学会第137回講演会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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