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Phenotypic analysis of newly generated c-kit mutant marmosets

Research Project

Project/Area Number 19K06462
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42040:Laboratory animal science-related
Research InstitutionCentral Institute for Experimental Animals

Principal Investigator

Kumita Wakako  公益財団法人実験動物中央研究所, マーモセット医学生物学研究部, 研究員 (50549128)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsコモンマーモセット / c-kit遺伝子 / ゲノム編集 / CRISPR/Cas9 / マーモセット / c-kit
Outline of Research at the Start

本研究はゲノム編集技術CRISPR/Cas9で作出したc-kit遺伝子変異コモンマーモセットの有用性を明らかにすることを目的とする。c-kit遺伝子は造血、生殖機能や毛色に関与し、自然突然変異体も含めて、その変異マーモセットは報告がない。作出したc-kit変異マーモセットは遺伝子変異のほか、毛色異常などc-kit変異マウスと同様の表現型が確認できている。さらに血液解析や、変異体が示すc-kit遺伝子配列の発現ベクターをもちいたリン酸化能解析により、個体の特性を明らかにする。またc-kit変異体細胞由来iPS細胞から始原生殖細胞誘導能を検討し、c-kit変異マーモセットの生殖能を明らかにする。

Outline of Final Research Achievements

Phenotypic analyses of c-kit gene-modified common marmosets (c-kit marmosets), generated by gene-editing technology, were performed. Hematopoietic function analysis showed no difference between c-kit and wild-type marmosets. To clarify the phosphorylation ability of mutant c-kit exhibited by the c-kit marmoset, we performed a phosphorylation assay using a mutant c-kit expression vector and a phosphorylated c-kit antibody. The results showed that one mutant c-kit shown by males and all mutant c-kit proteins shown by females lacked phosphorylation ability. For reproductive function analysis, semen was obtained from a male c-kit marmoset and analyzed, and the mutant c-kit gene was detected. In female c-kit marmosets, urinary progesterone levels were measured, suggesting possible infertility. This study indicates that the c-kit marmoset may be a helpful model in reproductive research. It is expected to contribute to establishing c-kit mutant primate models in the future.

Academic Significance and Societal Importance of the Research Achievements

c-kitマーモセットをもちいた本研究の結果から、c-kit変異マウスとは違う表現型が確認された。これは、霊長類ならではの表現型を見出した可能性が考えられ、ヒトへの応用が期待できる。また不妊のc-kitマーモセットは人工樹立した生殖細胞や、幼若生殖細胞の移植レシピエントに使用できると考えられ、不妊症の原因究明や治療法開発の研究や、霊長類実験動物の問題点である世代時間を短縮する研究などに貢献することが期待できる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (5 results)

All 2021 2020 2019

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (1 results) (of which Int'l Joint Research: 1 results) Book (2 results)

  • [Journal Article] An improved de novo genome assembly of the common marmoset genome yields improved contiguity and increased mapping rates of sequence data2020

    • Author(s)
      Jayakumar Vasanthan、Ishii Hiromi、Seki Misato、Kumita Wakako、Inoue Takashi、Hase Sumitaka、Sato Kengo、Okano Hideyuki、Sasaki Erika、Sakakibara Yasubumi
    • Journal Title

      BMC Genomics

      Volume: 21 Issue: S3 Pages: 243-243

    • DOI

      10.1186/s12864-020-6657-2

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing2019

    • Author(s)
      Kumita Wakako、Sato Kenya、Suzuki Yasuhiro、Kurotaki Yoko、Harada Takeshi、Zhou Yang、Kishi Noriyuki、Sato Kengo、Aiba Atsu、Sakakibara Yasubumi、Feng Guoping、Okano Hideyuki、Sasaki Erika
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 12719-12719

    • DOI

      10.1038/s41598-019-49110-3

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] マーモセットにおけるCBEシステムによる遺伝子改変評価2021

    • Author(s)
      汲田和歌子、笹栗弘貴、佐藤賢哉、大浦奈津希、西道隆臣、佐々木えりか
    • Organizer
      第10回日本マーモセット研究会大会
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research
  • [Book] ゲノム編集技術を応用した製品開発とその実用化2021

    • Author(s)
      汲田和歌子,佐々木えりか
    • Total Pages
      602
    • Publisher
      (株)技術情報協会
    • ISBN
      9784861048272
    • Related Report
      2020 Research-status Report
  • [Book] 週刊医学のあゆみ Vol.273 No.92020

    • Author(s)
      汲田和歌子、佐々木えりか
    • Total Pages
      232
    • Publisher
      医歯薬出版
    • Related Report
      2020 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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