Evolution of chlorophyll metabolic pathway

• Project/Area Number: 19K06700
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44030:Plant molecular biology and physiology-related
• Research Institution: Hokkaido University
• Principal Investigator: Tanaka Ayumi 北海道大学, 低温科学研究所, 名誉教授 (10197402)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 進化 / 代謝系 / クロロフィル

Outline of Research at the Start

物質、エネルギー代謝は生命活動の中心的な役割を担っている。それらを構成する酵素がどのように誕生し、新しい代謝経路が出現したのかは、生物進化の研究において最も興味深い問いの一つである。酵素は生理的な役割を担う触媒活性以外に、生理的には意味のない触媒活性（Promiscous activity)を併せ持っている。本研究の目的は、このPromiscousな活性がどのようにして新しい酵素の誕生へとつながったのかを明らかにし、酵素の誕生と進化のモデルを提案することである。

Outline of Final Research Achievements

Metabolism plays a central role in living organism. How the enzymes that constitute metabolic pathway developed and how new metabolic pathways emerged in the history of life is one of the most interesting questions in the study of evolution. In this study, it was found that the enzyme that degrades chlorophyll in plants is derived from a bacteria that is not related to chlorophyll degradation in the host cell. These results indicate that the bacterial gene was transferred to the ancestor of plants and its promiscuous activity of the enzyme was used to form a new metabolic pathway in plant.

Academic Significance and Societal Importance of the Research Achievements

学術的な意義:本研究は、酵素が潜在的に持っている活性が、進化の過程で新しい代謝系の構築にどのように利用されてきたかを調べたものである。クロロフィル代謝系を研究対象とし、生化学的な手法によりその解明に取り組んだ。この研究は、酵素が潜在的に持つ多様な活性という分子的な実体に基づいて代謝系の進化の解明に取り組んだ点に意義がある。
社会的な意義:本研究の成果は、遺伝子の改変による人工的な代謝系を利用した有用物質の生産などに応用できる可能性がある。

Research Products

• [Journal Article] Characterization of photosystem II assembly complexes containing ONE-HELIX PROTEIN1 in Arabidopsis thaliana (2022)
  • Author(s): Maeda Hanaki、Takahashi Koharu、Ueno Yoshifumi、Sakata Kei、Yokoyama Akari、Yarimizu Kozue、Myouga Fumiyoshi、Shinozaki Kazuo、Ozawa Shin-Ichiro、Takahashi Yuichiro、Tanaka Ayumi、Ito Hisashi、Akimoto Seiji、Takabayashi Atsushi、Tanaka Ryouichi
  • Journal Title: Journal of Plant Research Volume: 135 Issue: 2 Pages: 361-376
  • DOI: 10.1007/s10265-022-01376-x
  • Peer Reviewed
• [Journal Article] Unique Peripheral Antennas in the Photosystems of the Streptophyte Alga Mesostigma viride (2021)
  • Author(s): Aso Michiki、Matsumae Renon、Tanaka Ayumi、Tanaka Ryouichi、Takabayashi Atsushi
  • Journal Title: Plant and Cell Physiology Volume: 62 Issue: 3 Pages: 436-446
  • DOI: 10.1093/pcp/pcaa172
  • NAID: 120007182921
  • Peer Reviewed
• [Journal Article] How plants grow under gravity conditions besides 1 g: Perspectives from hypergravity and space experiments that employ bryophytes as a model organism (2021)
  • Author(s): Kume, A., Kamachi, H., Onoda, Y., Hanba, T.Y., Hiwatashi, Y., Karahara, I., and Fujita T.
  • Journal Title: Plant Molecular Biology Volume: - Issue: 2 Pages: 348-355
  • DOI: 10.1093/pcp/pcaa165
  • Peer Reviewed
• [Journal Article] Insights into the structure and function of the rate-limiting enzyme of chlorophyll degradation through analysis of a bacterial Mg-dechelatase homolog (2021)
  • Author(s): Dey Debayan、Dhar Dipanjana、Fortunato Helena、Obata Daichi、Tanaka Ayumi、Tanaka Ryouichi、Basu Soumalee、Ito Hisashi
  • Journal Title: Computational and Structural Biotechnology Journal Volume: 19 Pages: 5333-5347
  • DOI: 10.1016/j.csbj.2021.09.023
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Enrichment of chlorophyll catabolic enzymes in grana margins and their cooperation in catabolic reactions (2021)
  • Author(s): Fukura Koki、Tanaka Ayumi、Tanaka Ryouichi、Ito Hisashi
  • Journal Title: Journal of Plant Physiology Volume: 266 Pages: 153535-153535
  • DOI: 10.1016/j.jplph.2021.153535
  • Peer Reviewed
• [Journal Article] Degradation of the photosystem II core complex is independent of chlorophyll degradation mediated by Stay-Green Mg2+ dechelatase in Arabidopsis (2021)
  • Author(s): Chen Ying、Yamori Wataru、Tanaka Ayumi、Tanaka Ryouichi、Ito Hisashi
  • Journal Title: Plant Science Volume: - Pages: 110902-110902
  • DOI: 10.1016/j.plantsci.2021.110902
  • Peer Reviewed
• [Journal Article] Jasmonate production through chlorophyll a degradation by Stay-Green in Arabidopsis thaliana (2019)
  • Author(s): Ono Kouhei、Kimura Madoka、Matsuura Hideyuki、Tanaka Ayumi、Ito Hisashi
  • Journal Title: Journal of Plant Physiology Volume: 238 Pages: 53-62
  • DOI: 10.1016/j.jplph.2019.05.004
  • NAID: 120007099144
  • Peer Reviewed
• [Journal Article] Horizontal Transfer of Promiscuous Activity from Nonphotosynthetic Bacteria Contributed to Evolution of Chlorophyll Degradation Pathway (2019)
  • Author(s): Obata Daichi、Takabayashi Atsushi、Tanaka Ryouichi、Tanaka Ayumi、Ito Hisashi
  • Journal Title: Molecular Biology and Evolution Volume: 36 Issue: 12 Pages: 2830-2841
  • DOI: 10.1093/molbev/msz193
  • Peer Reviewed / Open Access
• [Journal Article] In Vitro Enzymatic Activity Assays Implicate the Existence of the Chlorophyll Cycle in Chlorophyll b-Containing Cyanobacteria (2019)
  • Author(s): Lim HyunSeok、Tanaka Ayumi、Tanaka Ryouichi、Ito Hisashi
  • Journal Title: Plant and Cell Physiology Volume: 60 Issue: 12 Pages: 2672-2683
  • DOI: 10.1093/pcp/pcz157
  • NAID: 120006876294
  • Peer Reviewed
• [Journal Article] Formation of a PSI-PSII megacomplex containing LHCSR and PsbS in the moss Physcomitrella patens (2019)
  • Author(s): Furukawa, R., Aso M, Fujita, T., Akimoto, S., Tanaka, R., Tanaka, A., Yokono, M., and Takabayashi, A.
  • Journal Title: Journal of Plant Research Volume: 132 Issue: 6 Pages: 867-880
  • DOI: 10.1007/s10265-019-01138-2
  • NAID: 120006941693
  • Peer Reviewed
• [Remarks] 植物の老化や紅葉には，バクテリアの遺伝子が関わっていた
  • URL: https://www.hokudai.ac.jp/news/190906_pr2.pdf