Investigation of novel pollen tube guidance factors and analysis of functional diversity of guidance factors

• Project/Area Number: 19K06706
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 44030:Plant molecular biology and physiology-related
• Research Institution: Nagoya University
• Principal Investigator: Kanaoka Masahiro 名古屋大学, 理学研究科, 講師 (10467277)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 植物有性生殖 / 花粉管ガイダンス / 受精 / 花粉管 / 胚珠 / トマト / シロイヌナズナ / 生殖隔離 / 進化 / 多様性

Outline of Research at the Start

細胞間コミュニケーションに関わる分子メカニズムを明らかにすることは、植物科学のみならず基礎生物学において重要な課題である。本研究では花粉管（♂）と雌しべ（♀）の細胞間でのコミュニケーションにおいて鍵となる花粉管誘引因子に着目する。本研究の学術的独自性は、複数の植物種を用いて、花粉管誘引因子の構造の共通性・多様性の解明を目指すところにあり、植物生殖研究に大きく貢献すると期待される。

Outline of Final Research Achievements

To maintain species, it is important for organisms to recognize gametophytes from same species for fertilization. In this research, I have investigated the following topics which are based on my previous research about pollen tube guidance in Torenia and Arabidopsis. (1) I have established in vitro pollen tube guidance assay system in tomato and Solanaceae species. (2) I have analyzed the function of female gametophyte- specific genes in tomato. (3) I have revealed functionally important (and not important) sequences in Torenia LURE proteins.

Academic Significance and Societal Importance of the Research Achievements

本研究により初めて、トマトにおいて培地上で花粉管が胚珠内へと誘引される系を作成することができた。これはナス科植物を用いて人工的に異種間交配などをするための基盤となると期待される。また、受精過程において発現する複数の遺伝子の機能を解析できたことにより、植物有性生殖について基礎的な知見が深まったと言える。

Research Products

• [Int'l Joint Research] ETH(スイス)
• [Int'l Joint Research] University of Zurich(スイス)
• [Journal Article] Chemical synthesis of <i>Torenia</i> plant pollen tube attractant proteins by KAHA ligation (2022)
  • Author(s): Kumarswamyreddy Nandarapu、Reddy Damodara N.、Robkis D. Miklos、Kamiya Nao、Tsukamoto Ryoko、Kanaoka Masahiro M.、Higashiyama Tetsuya、Oishi Shunsuke、Bode Jeffrey W.
  • Journal Title: RSC Chemical Biology Volume: 1 Issue: 6 Pages: 1-7
  • DOI: 10.1039/d2cb00039c
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparisons among populations and individuals to evaluate pollen?pistil interaction as a mechanism of reproductive interference in Taraxacum (2021)
  • Author(s): Hashimoto Keisuke、Yamamoto Akane、Kanaoka Masahiro M.、Naiki Akiyo、Takakura Ko-Ichi、Nishida Sachiko
  • Journal Title: Journal of Plant Research Volume: 135 Issue: 1 Pages: 29-40
  • DOI: 10.1007/s10265-021-01352-x
  • Peer Reviewed
• [Journal Article] Fine‐scale empirical data on niche divergence and homeolog expression patterns in an allopolyploid and its diploid progenitor species (2020)
  • Author(s): Akiyama Reiko、Sun Jianqiang、Hatakeyama Masaomi、Lischer Heidi E. L.、Briskine Roman V.、Hay Angela、Gan Xiangchao、Tsiantis Miltos、Kudoh Hiroshi、Kanaoka Masahiro M.、Sese Jun、Shimizu Kentaro K.、Shimizu‐Inatsugi Rie
  • Journal Title: New Phytologist Volume: 229 Issue: 6 Pages: 3587-3601
  • DOI: 10.1111/nph.17101
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Adaptive reduction of male gamete number in the selfing plant Arabidopsis thaliana (2020)
  • Author(s): Takashi Tsuchimatsu, Hiroyuki Kakui, Misako Yamazaki, Cindy Marona, Hiroki Tsutsui, Afif Hedhly, Dazhe Meng, Yutaka Sato, Thomas Studler, Ueli Grossniklaus, Masahiro M. Kanaoka, Michael Lenhard, Magnus Nordborg and Kentaro K. Shimizu
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 2885-2885
  • DOI: 10.1038/s41467-020-16679-7
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Comparison of mechanisms of reproductive interference in Taraxacum (2019)
  • Author(s): Takemori Akane、Naiki Akiyo、Takakura Ko-Ichi、Kanaoka Masahiro M、Nishida Sachiko
  • Journal Title: Annals of Botany Volume: 20 Issue: 6 Pages: 1-11
  • DOI: 10.1093/aob/mcz007
  • Peer Reviewed / Open Access
• [Presentation] 配偶体の機能や花粉管誘引に関わる遺伝子の単離と解析 (2022)
  • Author(s): 金岡雅浩
  • Organizer: 第63回日本植物生理学会年会
• [Presentation] Analysis of genes expressed in tomato female gametophytes (2021)
  • Author(s): Masahiro Kanaoka, Kana Hisabayashi, Tetsuya Higashiyama
  • Organizer: JSOL2021
  • Int'l Joint Research
• [Presentation] トマト胚珠で発現する遺伝子の解析と 花粉管誘引アッセイ系の開発 (2020)
  • Author(s): 金岡雅浩、久林加奈、東山哲也
  • Organizer: 第84回植物学会大会
• [Presentation] トマトにおける花粉管誘引アッセイ系の開発 (2020)
  • Author(s): 金岡雅浩
  • Organizer: 第61回植物生理学会年会
• [Patent(Industrial Property Rights)] 植物のin vitro受精システムの開発 (2019)
  • Inventor(s): 金岡雅浩
  • Industrial Property Rights Holder: 金岡雅浩
  • Industrial Property Rights Type: 特許
  • Filing Date: 2019