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Do emotional and motor conditionings share cerebellar circuit?

Research Project

Project/Area Number 19K06767
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44050:Animal physiological chemistry, physiology and behavioral biology-related
Research InstitutionHiroshima University

Principal Investigator

Yoshida Masayuki  広島大学, 統合生命科学研究科(生), 准教授 (70253119)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2019: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords情動 / 小脳 / キンギョ / ゼブラフィッシュ / 恐怖 / 不安 / 呼吸 / プルキンエ細胞 / 無条件刺激 / 登上線維 / 脳局所破壊 / 下オリーブ核 / EPSP / 魚類 / 学習
Outline of Research at the Start

キンギョの恐怖情動学習には小脳プルキンエ細胞の可塑的な変化が関わる。一方、運動性の学習にもこのニューロンの可塑性が重要であることが知られている。本研究では、「運動性の条件付けと情動性(恐怖)の条件付けとは同じ小脳神経回路を共有している」という仮説を検証し、その原理を明らかにする。さらに、反応獲得の時間経過が全く異なる情動性学習と運動性学習が、いかにして同じ小脳回路を使って実現するのかを解明する。手法として、条件付けに関わる情報伝達経路の遮断、神経活動の電気生理学的解析、新たな条件付け手続きの開発、を行う。本研究は、同一の脳内局所回路が複数のタスクに共有される原理の理解に貢献する。

Outline of Final Research Achievements

The aim of this research project was to determine whether fear emotional and motor learnings share cerebellar circuit in teleost fish. It has been found that unconditioned aversive stimulus is conveyed to the cerebellum via the inferior olive in the medulla. Both ipsilateral and contralateral lesions of the olivo-cerebellar tract negatively affected the performance of the classical fear conditioning. However, due to covid-19 pandemic situation, it was unable to properly supply experimental animals and difficult to proceed the project as expected. Nevertheless, modified project aiming to develop a technique to monitor the emotional state of the fish by means of measuring ventilatory activities in freely swimming fish was successfully conducted. The developed technique to monitor bioelectric signals derived from the ventilation activity was applied to examine the habituation process of the zebrafish in a novel environment.

Academic Significance and Societal Importance of the Research Achievements

脊椎動物における小脳は運動学習に深く関わることが明らかになっているが、近年恐怖などの情動性の学習にも重要であることが示されつつある。本研究では、基本的な恐怖学習である古典的恐怖条件付けにおいて、延髄の下オリーブ核が重要な役割を果たすことをキンギョをモデルとして明らかにした。さらに、ゼブラフィッシュをモデルとして、自由行動下における呼吸計測に基づく恐怖・不安情動の定量に成功した。またこれを応用して新奇環境におけるゼブラフィッシュの順化過程の記載に成功した。これらの成果は、情動の基本的神経機構とその進化の解明に貢献することが期待できる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (11 results)

All 2022 2021 2020 2019 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (5 results) (of which Invited: 3 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Recording the ventilation activity of free-swimming zebrafish and its application to novel tank tests2022

    • Author(s)
      Yoshida Masayuki
    • Journal Title

      Physiology & Behavior

      Volume: 244 Pages: 113665-113665

    • DOI

      10.1016/j.physbeh.2021.113665

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Measurement of emotional states of zebrafish through integrated analysis of motion and respiration using bioelectric signals2021

    • Author(s)
      Soh Zu、Matsuno Motoki、Yoshida Masayuki、Furui Akira、Tsuji Toshio
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 187-187

    • DOI

      10.1038/s41598-020-80578-6

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Relationship between brain morphology and life history in four bottom-dwelling gobiids2020

    • Author(s)
      Yoshida, M., Tsuji, T., Mukuda, T.
    • Journal Title

      Zoological Science

      Volume: 37 Issue: 2 Pages: 1680176-1680176

    • DOI

      10.2108/zs190109

    • NAID

      210000174021

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] Monitoring the ventilation activity of free-swimming zebrafish and its application to novel tank tests2021

    • Author(s)
      Yoshida Masayuki, Soh Zu
    • Organizer
      日本比較生理生化学会第43回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 自由行動中のゼブラフィッシュからの呼吸計測と新奇場面テストへの応用2021

    • Author(s)
      吉田将之
    • Organizer
      第92回日本動物学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Bold goldfish in behavioral neuroscience2021

    • Author(s)
      Yoshida Masayuki
    • Organizer
      The 2nd AsiaEvo Conference
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] サカナの行動と心理の比較生物学2019

    • Author(s)
      吉田将之
    • Organizer
      第21回 日本進化学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 脳と行動から探るサカナの心理2019

    • Author(s)
      吉田将之
    • Organizer
      同志社大学脳科学研究科リトリート
    • Related Report
      2019 Research-status Report
    • Invited
  • [Book] Death-Feigning in Insects, Mechanism and Function of Tonic Immobility2021

    • Author(s)
      Yoshida Masayuki
    • Total Pages
      181
    • Publisher
      Spriger
    • ISBN
      9789813365971
    • Related Report
      2021 Annual Research Report
  • [Book] Death-Feigning in Insects2021

    • Author(s)
      Sakai, M., Pfluter, HJ., Honma, A., Miyatake, T., Nakano, R., Takahashi, T., Nishono, H., Buschges , A., Namiki, S., Yoshida, M.
    • Total Pages
      181
    • Publisher
      Springer
    • ISBN
      9789813365988
    • Related Report
      2020 Research-status Report
  • [Remarks] 生物生産学部・教授に聞く

    • URL

      https://gsbstop.hiroshima-u.ac.jp/interview/ja/yoshida.html

    • Related Report
      2021 Annual Research Report

URL: 

Published: 2019-04-18   Modified: 2023-01-30  

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