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Identification of brood pouch-specific genes in seahorse

Research Project

Project/Area Number 19K06793
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 45020:Evolutionary biology-related
Research InstitutionSophia University

Principal Investigator

KAWAGUCHI Mari  上智大学, 理工学部, 准教授 (00612095)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Discontinued (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsbrood pouch / trasnposon / retroposon / seahorse / transposon / digestive enzyme / タツノオトシゴ / 育児嚢 / ヨウジウオ科
Outline of Research at the Start

動物の子育ては一般的にはメスが行っているが、タツノオトシゴはオスの育児嚢内で卵を保護し、その後、出産する。育児嚢は、どのような遺伝的な変化によって生じたものなのだろうか?進化過程で育児嚢という新奇な器官がどのように生じたのかを考察するために、本研究では以下の3点を明らかにする。第一に、タツノオトシゴの育児嚢の形成開始に関わる遺伝子を同定する。第二に、育児嚢を構成する組織のマーカー遺伝子を同定する。第三に、タツノオトシゴが属するヨウジウオ科魚種の育児嚢の組織観察をとおして、タツノオトシゴの育児嚢に特徴的な組織が種間で共通しているのかを明らかにする。

Outline of Final Research Achievements

Seahorses are one of teleostean fishes and have unique characteristics. One of them is that male seahorses possess a brood pouch on the ventral side of the body and protect their eggs in the pouch until parturition. Considering that brood pouch is uniquely found in their relatives, lineage-specific genes appeared during the evolution of seahorses.
In the present study, we newly found a gene expressed in flame cone cells uniquely found in species of the genus Hippocampus. The gene is translated into a protein possessing proline and glycine-rich sequences, and we named it "proline-glycine rich (PGrich) gene". The gene is estimated to be established from the antisense strand of the elastin gene by transposon or retroposon. Based on the analysis of genome sequences of various teleosts, we conclude that the gene emerged in the common ancestor of seahorse and pipefish.

Academic Significance and Societal Importance of the Research Achievements

育児嚢は、タツノオトシゴを含むヨウジウオ科魚類のすべてのオスに見られる子育て器官である。その形態は体表に付着させるだけの単純なものから袋状のものまで主に5つの形態に分類され、ヨウジウオ科魚類の進化過程で頻繁にその形態が変化している。近縁種でのみ見られる器官ということは、ゲノム配列のわずかな違いによって形態を多様化させていると考えられ、形態進化を詳細に調べることが可能な良い実験題材である。ヨウジウオ科魚種は藻場やサンゴ礁など生活環境に合わせて多様化しており、ヨウジウオ科魚種の育児嚢の多様性進化について理解できれば、生物の生き残り戦略の一端を解き明かすことになる。

Report

(4 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (23 results)

All 2022 2021 2020 2019 Other

All Journal Article (8 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 8 results,  Open Access: 5 results) Presentation (14 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Brood pouch evolution in pipefish and seahorse based on histological observation.2022

    • Author(s)
      Akari Harada, Ryotaro Shiota, Ryohei Okubo, Makiko Yorifuji, Atsushi Sogabe, Hiroyuki, Motomura, Junya Hiroi, Shigeki Yasumasu, and Mari Kawaguchi
    • Journal Title

      Placenta

      Volume: 120 Pages: 88-96

    • DOI

      10.1016/j.placenta.2022.02.014

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lineage‐specific evolution of zona pellucida genes in fish2022

    • Author(s)
      Sano Kaori、Shimada Sho、Mibu Hideki、Taguchi Mizuki、Ohsawa Takasumi、Kawaguchi Mari、Yasumasu Shigeki
    • Journal Title

      Journal of Experimental Zoology Part B: Molecular and Developmental Evolution

      Volume: 338 Issue: 3 Pages: 181-191

    • DOI

      10.1002/jez.b.23122

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular evolution of hatching enzymes and their paralogous genes in vertebrates.2022

    • Author(s)
      Tatsuki Nagasawa, Mari Kawaguchi, Kohki Nishi, Shigeki Yasumasu
    • Journal Title

      BMC Ecology and Evolution

      Volume: 22 Issue: 1 Pages: 9-9

    • DOI

      10.1186/s12862-022-01966-2

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Pactacin is a novel digestive enzyme in teleosts2021

    • Author(s)
      Kawaguchi M, Okazawa Y, Imafuku A, Nakano Y, Shimizu R, Ishizuka R, Jiang T, Nagasawa T, Hiroi J, Yasumasu S
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 7230-7230

    • DOI

      10.1038/s41598-021-86565-9

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RADSex: A computational workflow to study sex determination using restriction site‐associated DNA sequencing data2021

    • Author(s)
      R. Feron, Q. Pan, M. Wen, B. Imarazene, E. Jouanno, J. Anderson, A. Herpin, L. Journot, H. Parrinello, C. Klopp, V. A. Kottler, A. S. Roco, K. Du, S. Kneitz, M. Adolfi, C. A. Wilson, B. McCluskey, A. Amores, T. Desvignes, F. W. Goetz, A. Takanashi, M. Kawaguchi 他計36名
    • Journal Title

      Molecular Ecology Resources

      Volume: - Issue: 5 Pages: 1715-1731

    • DOI

      10.1111/1755-0998.13360

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Genome Sequence of the Euryhaline Javafish Medaka, Oryzias javanicus: A Small Aquarium Fish Model for Studies on Adaptation to Salinity2020

    • Author(s)
      Takehana Yusuke、Zahm Margot、Cabau Cedric、Klopp Christophe、Roques Celine、Bouchez Olivier、Donnadieu Cecile、Barrachina Celia、Journot Laurent、Kawaguchi Mari、Yasumasu Shigeki、Ansai Satoshi、Naruse Kiyoshi、Inoue Koji、Shinzato Chuya、Schartl Manfred、Guiguen Yann、Herpin Amaury
    • Journal Title

      G3 Genes|Genomes|Genetics

      Volume: 10 Issue: 3 Pages: 907-915

    • DOI

      10.1534/g3.119.400725

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Translocation of promoter-conserved hatching enzyme genes with intron-loss provides a new insight in the role of retrocopy during teleostean evolution.2019

    • Author(s)
      Tatsuki Nagasawa, Mari Kawaguchi, Tohru Yano, Sho Isoyama, Shigeki Yasumasu, and Masataka Okabe.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 2447-2447

    • DOI

      10.1038/s41598-019-38693-6

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Male parental assistance in embryo hatching of barred-chin blenny Rhabdoblennius nitidus2019

    • Author(s)
      Sano, K., Yokoyama, R., Kitano, T., Takegaki, T., Kitazawa, N., Kaneko, T., Nishino, Y., Yasumasu, S., Kawaguchi, M.
    • Journal Title

      J. Exp. Zool. B

      Volume: in press Issue: 3-4 Pages: 000-000

    • DOI

      10.1002/jez.b.22854

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] ヨウジウオ科魚類の育児嚢におけるアンモニア輸送の進化2022

    • Author(s)
      山崎大嬉・廣井準也・川口眞理
    • Organizer
      第74回日本動物学会関東支部
    • Related Report
      2021 Annual Research Report
  • [Presentation] Development of seahorse brood pouch in the aspect of neofunctionalization of the genes2021

    • Author(s)
      Mari Kawaguchi, Wen-Shan Chang, and Shigeki Yasumasu
    • Organizer
      4th SYNGBIO
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] タツノオトシゴの育児嚢におけるアンモニア輸送2021

    • Author(s)
      山﨑大嬉・廣井準也・川口眞理
    • Organizer
      第92回日本動物学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] カワスズメ科魚類における孵化腺細胞の局在の進化2020

    • Author(s)
      長澤竜樹・相原光人・川口眞理・二階堂雅人
    • Organizer
      第72回日本動物学会関東支部
    • Related Report
      2019 Research-status Report
  • [Presentation] ピグミーシーホースの抱卵時の腹腔の形態2020

    • Author(s)
      原田明里・本村浩之・川口眞理
    • Organizer
      第72回日本動物学会関東支部
    • Related Report
      2019 Research-status Report
  • [Presentation] タツノオトシゴの育児嚢 ~形成メカニズムを分子レベルで探る~2019

    • Author(s)
      川口眞理
    • Organizer
      第42回日本分子生物学会ワークショップ「生命のゆりかご 「胚体外」 の発生とエピゲノム」
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Unique molecular evolution in teleost hatching enzyme genes - is this a new role of retrotransposon for evolution?2019

    • Author(s)
      Tatsuki Nagasawa, Mari Kawaguchi, Tohru Yano, Sho Isoyama, Shigeki Yasumasu, Masataka Okabe
    • Organizer
      SMBE 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 孵化酵素遺伝子と卵膜遺伝子の共進化がもたらした魚類の多様な孵化機構2019

    • Author(s)
      佐野香織・川口眞理・安増茂樹
    • Organizer
      第90回日本動物学会シンポジウム「魚類の繁殖~多様すぎる形態と生理に分子生物学のメスを入れる~」
    • Related Report
      2019 Research-status Report
  • [Presentation] 真骨魚類レトロコピー遺伝子―十数億年続く分子進化の制約を打ち破る―2019

    • Author(s)
      長澤竜樹・川口眞理・矢野十織・安増茂樹・岡部正隆
    • Organizer
      第90回日本動物学会シンポジウム「遺伝的変異-発生・生理-適応度をつなぐ進化・多様性研究」
    • Related Report
      2019 Research-status Report
  • [Presentation] タツノオトシゴの育児嚢の形成へのFGFシグナルの関与2019

    • Author(s)
      原田明里・川原玲香・川口眞理
    • Organizer
      第90回日本動物学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 硬骨魚類孵化酵素の卵膜分解系の機能進化:正真骨魚類HCE-LCE系は、どのように成立したか?2019

    • Author(s)
      安増茂樹・川口眞理・竹野陸央・小島利恵子・松本透夜
    • Organizer
      第90回日本動物学会
    • Related Report
      2019 Research-status Report
  • [Presentation] タツノオトシゴの育児嚢の形成へのfgf10a・wnt5a遺伝子の関与2019

    • Author(s)
      原田明里・川原玲香・川口眞理
    • Organizer
      第52回日本魚類学会
    • Related Report
      2019 Research-status Report
  • [Presentation] 魚類には新規の消化酵素があるのか?2019

    • Author(s)
      川口眞理
    • Organizer
      第5回ユニーク会
    • Related Report
      2019 Research-status Report
  • [Presentation] 組織観察によるヨウジウオ科魚種の進化過程2019

    • Author(s)
      原田明里・川口眞理
    • Organizer
      第21回日本進化学会
    • Related Report
      2019 Research-status Report
  • [Remarks] 教員の受賞・成果

    • URL

      https://www.sophia.ac.jp/jpn/news/PR/210401_outcome.html

    • Related Report
      2020 Research-status Report

URL: 

Published: 2019-04-18   Modified: 2024-01-30  

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