Efffect of amygdalar activity on recognition and neural representation of facial information

• Project/Area Number: 19K07811
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 51020:Cognitive and brain science-related
• Research Institution: National Institutes for Quantum Science and Technology
• Principal Investigator: Miyakawa Naohisa 国立研究開発法人量子科学技術研究開発機構, 量子医科学研究所 脳機能イメージング研究部, 主任研究員 (60415312)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脳機能操作 / 情動 / 視覚 / 霊長類 / 神経操作 / 情動反応 / 顔認知 / 表情認知 / 腹側視覚皮質 / 扁桃体 / 電気生理 / 化学遺伝学 / 腹側視覚野 / 神経回路

Outline of Research at the Start

扁桃体が腹側視覚野とどのように協調して、顔の視覚情報処理を実現しているか、知覚、神経情報処理、神経回路の３つの側面から統合的に明らかにすることを目的とし、ヒト同様側頭葉が発達したマカクザルを対象として、申請者らがこれまで開発した広範囲で神経活動を計測するECoG記録法と、最新の化学遺伝学による脳活動操作とイメージングの融合技術によりアプローチする。本研究の成果は、現状の顔視覚情報処理の理解において、パラダイムシフトをもたらすとともに、ヒトのコミュニケーションの神経機構やその破綻の理解、そしてヒトの認知機能を模す人工知能への応用においても重要な意義をもたらす。

Outline of Final Research Achievements

In this study, we used "chemogenetics," which is rarely used in the world, to intervene the local brain activity in macaque monkeys in order to evaluate the effects of the amygdala, the center of emotional processing in the brain, on the ventral visual cortex, the center of visual information processing. In the first half of the study, we succeeded in developing a new drug that dramatically increases the function of "chemogenetics". In the second half of the study, we found that actual amygdala manipulations altered the animals' emotional responses as measured by pupil aperture and the neural representation of emotional visual perception as measured by electrocorticography (electroencephalography beneath the dura matter.

Academic Significance and Societal Importance of the Research Achievements

私たちヒトの社会的コミュニケーションを支える顔の視覚認知処理の神経機構は、腹側視覚皮質を中心に理解が進められてきた。一方、これまで情動に関連する脳領域とされてきた扁桃体が、短潜時で顔検知が可能であると報告されるなど、顔認知情報処理に重要な役割を果たす可能性が示されている。扁桃体は腹側視覚野とどのように協調して、顔の視覚情報処理を実現しているのだろうか？ 本研究はこの問いについて、脳の回路の機能操作とその結果の分析により、知覚、神経情報処理、神経回路の３つの側面から統合的かつ帰納的な解を与えることに成功した。

Research Products

• [Int'l Joint Research] University of North Carolina/Mount Sinai Cntr Therapeut Discov(米国)
• [Int'l Joint Research] University of North Carolina/Mount Sinai Cent. Therapeutic Discovery(米国)
• [Journal Article] Chronic Behavioral Manipulation via Orally Delivered Chemogenetic Actuator in Macaques (2022)
  • Author(s): Oyama Kei、Hori Yukiko、Nagai Yuji、Miyakawa Naohisa、Mimura Koki、Hirabayashi Toshiyuki、Inoue Ken-ichi、Takada Masahiko、Higuchi Makoto、Minamimoto Takafumi
  • Journal Title: The Journal of Neuroscience Volume: 42 Issue: 12 Pages: 2552-2561
  • DOI: 10.1523/jneurosci.1657-21.2021
  • Peer Reviewed / Open Access
• [Journal Article] Chemogenetic dissection of the primate prefronto-subcortical pathways for working memory and decision-making (2021)
  • Author(s): Oyama Kei、Hori Yukiko、Nagai Yuji、Miyakawa Naohisa、Mimura Koki、Hirabayashi Toshiyuki、Inoue Ken-ichi、Suhara Tetsuya、Takada Masahiko、Higuchi Makoto、Minamimoto Takafumi
  • Journal Title: Science Advances Volume: 7 Issue: 26 Pages: 1-9
  • DOI: 10.1126/sciadv.abg4246
  • NAID: 120007089253
  • Peer Reviewed / Open Access
• [Journal Article] Chemogenetic dissection of the primate prefronto-subcortical pathways for working memory and decision-making (2021)
  • Author(s): Kei Oyama, Yukiko Hori, Yuji Nagai, Naohisa Miyakawa, Koki Mimura, Toshiyuki Hirabayashi, Ken-ichi Inoue, Tetsuya Suhara, Masahiko Takada, Makoto Higuchi, Takafumi Minamimoto
  • Journal Title: Science Advances (in press) Volume: －
  • NAID: 120007089253
  • Peer Reviewed / Open Access
• [Journal Article] Deschloroclozapine, a potent and selective chemogenetic actuator enables rapid neuronal and behavioral modulations in mice and monkeys (2020)
  • Author(s): *Nagai Y, *Miyakawa N, Takuwa H, Hori Y, Oyama K, Ji B, Takahashi M, Huang XP, Slocum S, DiBerto JF, Xiong Y, Urushihata T, Hirabayashi T, Fujimoto F, Mimura K, English JG, Liu J, Inoue K, Kumata K, Seki C, Ono M, Shimojo M, Zhang MR, Tomita Y, Nakahara J, Suhara T, Takada M, Higuchi M, Jin J, Roth BL, Minamimoto T
  • Journal Title: Nature Neuroscience Volume: -
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Chemogenetic suppression of pharmacologically induced frontal lobe epilepsy in a macaque monkey (2021)
  • Author(s): Miyakawa N, Nagai Y, Kawasaki K, Hori Y, Oyama K, Orihara A, Matsuo T, Suzuki T, Inoue K, Takada M, Suhara T, Higuchi M, Minamimoto T
  • Organizer: 2021 American Epilepsy Society Annual Meeting
  • Int'l Joint Research
• [Presentation] Chemogenetic suppression of pharmacologically-induced epilepsy in a macaque monkey (2021)
  • Author(s): Miyakawa N, Nagai Y, Kawasaki K, Hori Y, Oyama K, Orihara A, Matsuo T, Suzuki T, Inoue K, Takada M, Suhara T, Higuchi M, Minamimoto T
  • Organizer: 44th Annual Meeting of the Japan Neuroscience Society
  • Int'l Joint Research
• [Presentation] Effects of differences in facial surface properties on temporal cortical neuronal activity and discrimination learning in macaque monkeys (2021)
  • Author(s): Kazuko Hayashi, Narihisa Matsumoto, Keiji Matsuda, Kenichiro Miura, Shigeru Yamane, Shin Matsuo, Keiji Yanai, Mark A. G. Eldridge, Richard C. Saunders, Barry J. Richmond, Yuji Nagai, Naohisa Miyakawa, Takafumi Minamimoto, Masato Okada, Kenji Kawano, Yasuko Sugase-Miyamoto
  • Organizer: 44th Annual Meeting of the Japan Neuroscience Society
  • Int'l Joint Research
• [Presentation] 扁桃体が腹側視覚皮質における社会・情動性の視覚情報表現に果たす役割 ～化学遺伝学神経操作によるアプローチ (2020)
  • Author(s): Naohisa Miyakawa, Yuji Nagai, Yukiko Hori, Takeshi Matsuo, Takafumi Suzuki, Ken-ichi Inoue, Toshiyuki Hirabayashi, Kei Oyama, Masahiko Takada, Tetsuya Suhara, Makoto Higuchi, Keisuke Kawasaki, Takafumi Minamimoto
  • Organizer: 第43回日本神経科学大会
• [Presentation] Effects of different physical surface properties on face discrimination learning in macaque monkeys (2020)
  • Author(s): Kazuko Hayashi, Narihisa Matsumoto, Keiji Matsuda, Kenichiro Miura, Shigeru Yamane, Shin Matsuo, Keiji Yanai, Mark A. G. Eldridge, Richard C. Saunders, Barry J. Richmond, Yuji Nagai, Naohisa Miyakawa, Takafumi Minamimoto, Masato Okada, Kenji Kawano, Yasuko Sugase-Miyamoto
  • Organizer: 日本動物心理学会 第80回大会
• [Presentation] 扁桃体の持続的賦活化により腹側視覚皮質の顔‐表情の神経表現が変容する (2020)
  • Author(s): 宮川 尚久
  • Organizer: 2020生理研研究会「行動の多様性を支える神経基盤とその動作様式の解明」
  • Invited
• [Presentation] 扁桃体神経活動が腹側視覚野における他者の顔・表情の神経表現に及ぼす影響 (2019)
  • Author(s): 宮川 尚久, 永井 裕司, 堀 由紀子, 南本 敬史
  • Organizer: 生理研研究会「行動の多様性を支える神経基盤とその動作様式の解明」
• [Presentation] Amygdala dysfunction specifically disrupts socio-emotional representation in macaque ventral visual cortex (2019)
  • Author(s): Miyakawa N, Nagai Y, Hori Y, Matsuo T, Suzuki T, Inoue K, Takada M, Suhara T, Kawasaki, K, Minamimoto T
  • Organizer: International Symposium "Past, Present and Future of Shitsukan Science and Technologies"
  • Int'l Joint Research
• [Presentation] DREADD inactivation reveals critical role of orbitofrontal cortex in reward-based adaptive decision-making in monkeys (2019)
  • Author(s): Oyama K, Hori Y, Nagai Y, Hirabayashi, T, Miyakawa N, Fujimoto A, Mimura K, Inoue K, Eldridge M, Saunders R, Suhara T, Takada M, Masato, H, Richmond, B, Minamimoto T
  • Organizer: 第42回 日本神経科学大会
  • Int'l Joint Research
• [Presentation] Neural coding of facial images with different skin textures in the temporal lobe of monkey (2019)
  • Author(s): Hayashi K, Matsumoto N, Matsuda K, Kenichro Miura, Yamane S, Matsuo S, Yanai K, Eldridge M A G, Saunders R C, Richmond B J, Nagai Y, Miyakawa N, Minamimoto T, Okada M, Kawano K, Sugase-Miyamoto Y
  • Organizer: 第42回 日本神経科学大会
  • Int'l Joint Research
• [Presentation] Chemogenetic activation of the amygdala specifically disrupts the representation of socio-emotional information in the macaque ventral visual cortex (2019)
  • Author(s): Miyakawa N, Nagai Y, Hori Y, Matsuo T, Suzuki T, Inoue K, Takada M, Suhara T, Kawasaki, K, Minamimoto T
  • Organizer: 第42回 日本神経科学大会
  • Int'l Joint Research
• [Presentation] A novel ligand “deschloroclozapine” selectively visualizes and activates chemogenetic receptors in non-human primates (2019)
  • Author(s): Nagai Y., Miyakawa N, Takuwa H., Hori Y., Oyama K., Ji B., Takahashi M., Huang X.-P., Slocum S. T., Xiong Y., Hirabayashi T., Fujimoto A., Mimura K., English J. G., Liu J., Inoue K.-i., Kumata K., Seki C., Ono M., Shimojo M., Zhang M.-R., Tomita Y., Suhara T., Takada M., Higuchi M., Jin J., Roth B. L., and Minamimoto T
  • Organizer: Brain and BrainPET 2019
  • Int'l Joint Research
• [Book] 実験医学 2020年11月号、担当範囲:「新しいDREADDsアゴニストDCZと霊長類におけるDREADDs実験例」 (2020)
  • Author(s): 宮川尚久(分担執筆)
  • Total Pages: 9
  • Publisher: 羊土社
  • ISBN: 9784758125376
• [Remarks] 脳の「スイッチ」にピンポイントで作用する薬剤候補を開発
  • URL: https://www.qst.go.jp/site/press/42113.html
• [Remarks] 神経活動を操作する新技術『化学遺伝学』に、飛躍的に性能が向上した薬剤が登場
  • URL: https://www.jnss.org/news-topics?id=210127-01
• [Patent(Industrial Property Rights)] 人工受容体に結合する新規化合物、人工受容体のイメージング方法、アゴニストまたはアンタゴニスト、治療薬、コンパニオン診断薬、神経細胞のイメージング方法 (2021)
  • Inventor(s): 南本敬史, 永井裕司, 季斌, 宮川尚久, 樋口真人, 須原哲也
  • Industrial Property Rights Holder: 国立研究開発法人量子科学技術研究開発機構
  • Industrial Property Rights Type: 特許
  • Filing Date: 2021
  • Acquisition Date: 2021