Development of the early diagnostic method of intractable ITP using single nucleotide polymorphism analysis of the cytokine gene and EV
Project/Area Number |
19K07948
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 52010:General internal medicine-related
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Research Institution | Kansai Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
伊藤 量基 関西医科大学, 医学部, 准教授 (70434826)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | ITP / SNP / EV / サイトカイン遺伝子 / 樹状細胞 |
Outline of Research at the Start |
難治性の免疫性血小板減少症(ITP)に関する対策は未だ完全ではない。本研究では、難治性ITPを早期に診断して適切な治療ストラテジーを構築するために、Extracellular vesicle (EV)とサイトカイン遺伝子の一塩基多型(SNP)解析を組み合わせた早期診断システムの開発を目的としている。EVは、細胞の活性化やアポト-シスの結果生成される物質であり、生体における恒常性維持や様々な病態の発症メカニズムに関与している。サイトカイン遺伝子のSNPは多くの自己免疫疾患の病態に関わっており、海外ではITPの病態に関する検討が一部報告されている。
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Outline of Final Research Achievements |
We analized the structure of EV derived from THP-1, the dendritic cell instruction of immunity and cytokine production. The cytokine-related SNP analysis in ITP patients revealed that low frequency of TGF-β1 +869 C/C genotype compared to normal controls. About the treatment reactivity to ITP, a constant tendency was provided in reactivity and cytokine genetic polymorphism of a steroid and the thrombopoietin receptor stimulating medicine (eltrombopag). These results suggested that it was more likely to be tied to early checkup of intractable ITP and the construction of the appropriate treatment strategy by incorporating reactivity of the treatment and EV-related data.
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Academic Significance and Societal Importance of the Research Achievements |
ITP患者のTGF-β1 +869 C/C genotype の頻度が健常コントロールに比較して有意に低値であることが判明した。またITPに対する治療反応性に関してもステロイドおよびトロンボポエチン受容体作動薬(エルトロンボパグ)の反応性とサイトカイン遺伝子多型との関連性について新たな知見を得ることができた。活性化したTHP-1からのEVの構造解析、ITPにおける標的細胞の検討と並行して、EVの樹状細胞免疫誘導ならびにサイトカイン産生が明らかとなった。以上より、治療の反応性とEVデータの組み込みによって、難治性ITPの早期診断および適切な治療ストラテジーの構築が可能となることが示唆された。
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Report
(4 results)
Research Products
(30 results)
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[Journal Article] Evaluation of azacitidine in patients with transplant-ineligible myelodysplastic syndromes and acute myeloid leukemia with myelodysplasia-related changes in a Japanese clinical setting2019
Author(s)
Nakaya Aya, Fujita Shinya, Satake Atsushi, Nakanishi Takahisa, Azuma Yoshiko, Tsubokura Yukie, Saito Ryo, Konishi Akiko, Hotta Masaaki, Yoshimura Hideaki, Ishii Kazuyoshi, Ito Tomoki, Nomura Shosaku
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Journal Title
Oncology Letters
Volume: 19
Pages: 1317-1321
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Trend of salvage treatment in diffuse large B cell lymphoma in the outpatient chemotherapy era2019
Author(s)
Nakaya Aya, Fujita Sshinya, Satake Atsushi, Nakanishi Takahisa, Azuma Yoshiko, Tsubokura Yukie, Saito Ryo, Konishi Akiko, Hotta Masaaki, Yoshimura Hideaki, Ishii Kazuyoshi, Ito Tomoki, Nomura Shosaku
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Journal Title
Molecular and Clinical Oncology
Volume: 19
Pages: 557-562
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The immunomodulatory-drug, lenalidomide, sustains and enhances interferon-α production by human plasmacytoid dendritic cells2019
Author(s)
Kibata Kayo, Ito Tomoki, Inaba Muneo, Tanaka Akihiro, Iwata Ryoichi, Inagaki-Katashiba Noriko, Phan Vien, Satake Atsushi, Nomura Shosaku
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Journal Title
Jounal of Blood Medicine
Volume: 10
Pages: 217-226
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Evaluation of eltrombopag in patients with aplastic anemia in real-world experience2019
Author(s)
Konishi Akiko, Nakaya Aya, Fujita Shinya, Satake Atsushi, Nakanishi Takahisa, Azuma Yoshiko, Tsubokura Yukie, Saito Ryo, Hotta Masaaki, Yoshimura Hideaki, Ishii Kazuyoshi, Ito Tomoki, Nomura Shosaku
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Journal Title
Leukemia Research Reports
Volume: 11
Pages: 11-13
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Myodesopsia is a symptom of central nervous sysyem blast crisis in chronic myeloid leukemia2019
Author(s)
Ishii Kazuyoshi, Nakaya Aya, Fujita Shinya, Satake Atsushi, Azuma Yoshiko, Tsubokura Yukie, Saito Ryo, Konishi Akiko, Hotta Masaaki, Yoshimura Hideaki, Ito Tomoki, Nomura Shosaku
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Journal Title
Leukemia Research Reports
Volume: 11
Pages: 8-10
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Discovery of a Small Molecule to Increase Cardiomyocytes and Protect the Heart After Ischemic Injury2018
Author(s)
Hara H, Takeda N, Kondo M, Kubota M, Saito T, Maruyama J, Fujiwara T, Maemura S, Ito M, Naito AT, Harada M, Toko H, Nomura S, Kumagai H, Ikeda Y, Ueno H, Takimoto E, Akazawa H, Morita H, Aburatani H, Hata Y, Uchiyama M, Komuro I.
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Journal Title
JACC: Basic to Translational Science
Volume: 3
Issue: 5
Pages: 639-653
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Efficacy of CMV prophylaxis with letermovir early after transplantation2019
Author(s)
Satake Atsushi, Saito Ryo, Konishi Akiko, Yoshimura Hideaki, Hotta Masaaki, Nakanishi Takahisa, Fujita Shinya, Nakaya Aya, Ito Tomoki, Ishii Kazuyoshi, Nomura Shosaku
Organizer
45th ASH Annual Meeting of the EBMT
Related Report
Int'l Joint Research
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