Development of functional muscle sheet using direct conversion technique and iBTA
| Project/Area Number |
19K09053
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 55010:General surgery and pediatric surgery-related
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Fumino Shigehisa 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40405254)
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| Co-Investigator(Kenkyū-buntansha) |
松田 修 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00271164)
岸田 綱郎 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00370205)
坂井 宏平 京都府立医科大学, 医学(系)研究科(研究院), 客員講師 (30515292)
田尻 達郎 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80304806)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 骨格筋シート / バイオシート / ダイレクトリプログラミング / direct conversion / MYOD1 / L-MYC |
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| Outline of Research at the Start |
先天性骨格筋欠損に対しての骨格筋シートの作成を最終目標とする．線維芽細胞を高効率に多核筋細胞に形質転換するダイレクトリプログラミング法と生体内組織形成技術により作成したバイオシートを用いることで，マウス腹壁欠損モデルについて，自己細胞・組織から強度と運動性を担保した「機能する」骨格筋シートを短期間に作成し，実臨床において応用可能な治療プラットフォームの開発を目指す．
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| Outline of Final Research Achievements |
In order to create skeletal muscle sheets for congenital skeletal muscle defects, we have developed a short time frame to create "functional" skeletal muscle sheets in a mouse abdominal wall defect model by using a direct programming method that efficiently transforms fibroblasts into multinuclear muscle cells and biosheets created by in vivo tissue formation technology.
Specifically, multinucleated skeletal muscle cells differentiated from human fibroblasts by direct conversion were seeded onto a mouse abdominal wall defect model using a biosheet fabricated subcutaneously in mice as a scaffold, and transplanted into the mouse abdominal wall defect model for histological evaluation. The results showed some growth, but not enough. In vivo transplantation experiments using cross-linked nanogels will be performed in the future.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では，先天性骨格筋欠損に対しての骨格筋シートの作成を最終目標とする.線維芽細胞を高効率に多核筋細胞に形質転換するダイレクトリプログラミング法と生体内組織形成技術により作成したバイオシートを用いることで，マウス腹壁欠損モデルについて，自己細胞・組織から強度と運動性を担保した「機能する」骨格筋シートを短期間に作成し，実臨床において応用可能な治療プラットフォームの開発を目指す.
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Report
(4 results)
Research Products
(72 results)
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[Journal Article] Novel Two MRT Cell Lines Established from Multiple Sites of a Synchronous MRT Patient2020
Author(s)
Yasumichi Kuwahara, Tomoko Iehara, Eisuke Ichise, Yoshiki Katsumi, Kazutaka Ouchi, Kunihiko Tsuchiya, Mitsuru Miyachi, Eiichi Konishi, Hiroyasu Sasajima, Satoaki Nakamura, Shigehisa Fumino, Tatsuro Tajiri, Pascal D Johann, Michael Fruhwald, Tsukasa Okuda, Hajime Hosoi
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Journal Title
Anticancer Research
Volume: 40
Issue: 11
Pages: 6159-6170
DOI
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Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Japanese Clinical Practice Guidelines for Sacrococcygeal Teratoma, 20172019
Author(s)
Fumino S, Tajiri T, Usui N, Tamura M, Sago H, Ono S, Nosaka S, Yoneda A, Souzaki R, Higashi M, Sakai K, Takahashi K, Sugiura T, Taguchi T
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Journal Title
Pediatrics International
Volume: 61
Issue: 7
Pages: 672-678
DOI
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Peer Reviewed / Open Access
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[Journal Article] Efficacy and safety of sirolimus treatment for intractable lymphatic anomalies: A study protocol for an open-label, single-arm, multicenter, prospective study (SILA).2019
Author(s)
Ozeki M, Asada R, Saito AM, Hashimoto H, Fujimura T, Kuroda T, Ueno S, Watanabe S, Nosaka S, Miyasaka M, Umezawa A, Matsuoka K, Maekawa T, Yamada Y, Fujino A, Hirakawa S, Furukawa T, Tajiri T, Kinoshita Y, Souzaki R, Fukao T
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Journal Title
Regen Ther.
Volume: 14
Pages: 84-91
DOI
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Peer Reviewed / Open Access
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[Journal Article] Results of a prospective clinical trial JN-L-10 using image-defined risk factors to inform surgical decisions for children with low-risk neuroblastoma disease: A report from the Japan Children’s Cancer Group Neuroblastoma Committee2019
Author(s)
Iehara T, Yoneda A, Yokota I, Takahashi H, Teramukai S, Kamijyo T, Nakazawa A, Takimoto T, Kikuta A, Yagyu S, Ikeda H, Nakagawara A, Tajiri T
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Journal Title
Pediatr Blood Cancer
Volume: 66
Issue: 11
Pages: 1-7
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Enhanced metastatic growth after local tumor resection in the presence of synchronous metastasis in a mouse allograft model of neuroblastoma2019
Author(s)
Inoue M, Tsuchiya Y, Koike N, Umemura Y, Inokawa H, Togashi Y, Maniwa J, Higashi M, Fumino S, Tajiri T, Yagita K
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Journal Title
Pediatric Surgery International
Volume: 35
Issue: 12
Pages: 1403-1411
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] 神経芽腫マウスにおける神経芽腫選択的な新規間葉系幹細胞デリバリーシステム2019
Author(s)
Maniwa J, Fimino S, Kimura K, Tanaka T, Higashi M, Sakai K, Aoi S, Furukawa T, Kishida T, Mazda O, Tajiri T
Organizer
第57回日本癌治療学会学術集会
Related Report
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