Exploration for the mechanisms of cell therapy by encapsulated cell transplantation
Project/Area Number |
19K09528
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Review Section |
Basic Section 56010:Neurosurgery-related
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Research Institution | Okayama University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
黒住 和彦 浜松医科大学, 医学部, 教授 (20509608)
亀田 雅博 大阪医科薬科大学, 医学部, 特別職務担当教員(講師) (50586427)
菱川 朋人 岡山大学, 医歯薬学域, 講師 (60509610)
佐々木 達也 岡山大学, 大学病院, 助教 (80790865)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Project Status |
Completed (Fiscal Year 2021)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | 幹細胞 / 移植治療 / カプセル化細胞 / うつ病 / 脳梗塞 / 神経新生 / うつ様症状 / 骨髄由来幹細胞 / 脳血流評価 / うつ症状 / 細胞療法 |
Outline of Research at the Start |
ヒト間葉系幹細胞由来多能性幹細胞を用いて、凍結保存の状態から溶解し直接移植する群とカプセル化細胞移植する群に分けて治療効果を検討する。対象とするのは一過性中大脳動脈閉塞モデルラットであり、行動学的評価・組織学的評価を中心に行う。うつ様症状に対する治療効果も重要な評価項目とする。虚血の程度をエコーにより確認し、血流遮断時の虚血負荷、運動機能、うつ様症状、組織学的評価(脳梗塞体積、炎症性変化、神経新生の程度等)を個体ごとに確認し解析する。過去に、脳梗塞モデル動物で直接移植とカプセル化移植の比較を行ったことはなく、細胞治療の意義・作用機序の解明に迫る研究である。
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Outline of Final Research Achievements |
HMGB1 (High Mobility Group Box-1) administration enhanced angiogenesis in indirect vascular reconstruction for chronic ischemia model rats. Depression-like behavior of Wistar Kyoto rats were improved by encapsulated rat bone marrow-derived mesenchymal stromal cell transplantation into the lateral ventricle. The improvement of depression-like behavior were related to enhanced neurogenesis in the hippocampus. Encapsulated transplantation and direct transplantation of human bone marrow-derived multipotent stem cells into the brain showed motor function recovery and reduction of the infarct volumes. In encapsulated cell transplantation group, stronger neurogeneic potentials were shown by the increase of surviving cells. Depression-like behavior was improved in the both treatment groups without significant differences.
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Academic Significance and Societal Importance of the Research Achievements |
カプセル化細胞移植により、長期間の細胞生存が見込まれ、それにより神経新生が増強されたことは、実臨床の移植方法を考える上でも有益な情報である。また、直接細胞移植と同等の治療効果がカプセル化細胞移植で示されたことは、骨髄由来の様々な細胞が実臨床で用いられているが、その治療効果として様々な栄養因子が極めて大きな役割を果たしているということを示している。うつ様行動が細胞移植によって改善を示したことは、多くのうつ病患者の新しい治療として、細胞療法が重要な役割を果たすことを明らかにしており、今後の臨床応用への重要な一歩になったと考えている。
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Report
(4 results)
Research Products
(209 results)
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[Journal Article] Pyogenic Ventriculitis After Anterior Skull Base Surgery Treated With Endoscopic Ventricular Irrigation And Reconstruction Using a Vascularized Flap2021
Author(s)
Tomita Y , Shimazu Y , Kawakami M , Matsumoto H , Fujii K , Kameda M , Yasuhara T , Suruga Y , Ota T , Kimata Y , Kurozumi K , Date I
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Journal Title
Acta Medica Okayama
Volume: 75
Issue: 2
Pages: 243-248
DOI
NAID
URL
Related Report
Peer Reviewed / Open Access
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[Journal Article] Trends in incidence of intracranial and spinal arteriovenous shunts: Hospital-based surveillance in Okayama, Japan2021
Author(s)
Murai S , Hiramatsu M , Suzuki E , Ishibashi R , Takai H , Miyazaki Y , Takasugi Y , Yamaoka Y , Nishi K , Takahashi Y , Harum J , Hishikawa T , Yasuhara T , Chin M , Matsubara S , Uno M , Tokunaga K , Sugiu K , Date I , on behalf of the Okayama Cranial and Spinal A-V shunts (OCSS) Study Group
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Journal Title
Stroke
Volume: 52(4)
Issue: 4
Pages: 1455-1459
DOI
Related Report
Peer Reviewed
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[Journal Article] An Evaluation of the Safety and Feasibility of Adenosine-assisted Clipping Surgery for Unruptured Cerebral Aneurysms: Study Protocol2021
Author(s)
Hishikawa T , Murai S , Hiramatsu M , Haruma J , Nishi K , Ebisudani Y , Sato Y , Yasuhara T , Sugiu K , Shimizu K , Kobayashi M , Nakagawa K , Kimura-Ono A , Hotta K , Morimatsu H , Date I
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Journal Title
Neurologia medico-chirurgica
Volume: 61
Issue: 7
Pages: 393-396
DOI
NAID
ISSN
0470-8105, 1349-8029
Related Report
Peer Reviewed / Open Access
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[Journal Article] Vagus nerve stimulation with mild stimulation intensity exerts anti-inflammatory and neuroprotective effects in Parkinson's disease model rats2021
Author(s)
Kin I , Sasaki T , Yasuhara T , Kameda M , Agari T , Okazaki M , Hosomoto K , Okazaki Y , Yabuno S , Kawauchi S , Kuwahara K , Morimoto J , Kin K , Umakoshi M , Tomita Y , Tajiri N , Borlongan CV , Date I
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Journal Title
Biomedicines
Volume: 9(7):789
Issue: 7
Pages: 789-789
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Incidence and clinical characteristics of spinal arteriovenous shunts: hospital-based surveillance in Okayama, Japan2021
Author(s)
Hiramatsu M , Ishibashi R , Suzuki E , Miyazaki Y , Murai S , Takai H , Takasugi Y , Yamaoka Y , Nishi K , Takahashi Y , Haruma J , Hishikawa T , Yasuhara T , Chin M , Matsubara S , Uno M , Tokunaga K , Sugiu K , Date I
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Journal Title
Journal of Neurosurgery Spine
Volume: ー
Issue: 4
Pages: 670-677
DOI
Related Report
Peer Reviewed
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[Journal Article] Long-term continuous cervical spinal cord stimulation exerts neuroprotective effects in experimental Parkinson's disease2020
Author(s)
Kuwahara K , Sasaki T , Yasuhara T , Kameda M , Okazaki Y , Hosomoto K , Kin I , Okazaki M , Yabuno S , Kawauchi S , Tomita Y , Umakoshi M , Kin K , Morimoto J , Lee J-Y , Tajiri N , Borlongan CV , Date I
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Journal Title
Frontiers in Aging Neuroscience
Volume: 16;12:164.
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cell therapy for central nervous system disorders: Current obstacles to progress2019
Author(s)
Yasuhara T , Kawauchi S , Kin K , Morimoto J , Kameda M , Sasaki T , Bonsack B , Kingsbury C , Tajiri N , Borlongan CV , Date I
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Journal Title
CNS Neuroscience & Therapeutics
Volume: ー
Issue: 6
Pages: 592-602
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] High-mobility group box-1-induced angiogenesis after indirect bypass surgery in a chronic cerebral hypoperfusion model2019
Author(s)
Nishihiro S , Hishikawa T , Hiramatsu M , Kidani N , Takahashi Y , Murai S , Sugiu K , Higaki Y , Yasuhara T , Borlongan CV , Date I
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Journal Title
Neuromoloecular Medicine
Volume: 21
Issue: 4
Pages: 391-400
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Lithium counteracts depressive behavior and augments the treatment effect of selective serotonin reuptake inhibitor in treatment-resistant depressed rats2019
Author(s)
Kin K, Yasuhara T, Kawauchi S, Kameda M, Hosomoto K, Tomita Y, Umakoshi M, Kuwahara K, Kin I, Kidani N, Morimoto J, Sasaki T, Date I
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Journal Title
Brain Research
Volume: 1717
Pages: 52-59
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of somatotopic organization and optimal stimulation site within the subthalamic nucleus for Parkinson's disease2019
Author(s)
Sasaki T , Kuwahara K , Kin I , Okazaki M , Sasada S , Shinko A , Kameda M , Yasuhara T , Agari T , Date I
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Journal Title
Operative Neurosurgery (Hagerstown, Md.)
Volume: 17(3)
Issue: 3
Pages: 239-246
DOI
Related Report
Peer Reviewed
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