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Regulation of sperm capacitation through control of sperm membrane sterol levels by seminal vesicle secretory proteins.

Research Project

Project/Area Number 19K09721
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56030:Urology-related
Research InstitutionToin University of Yokohama

Principal Investigator

Yoshida Kaoru  桐蔭横浜大学, 医用工学部, 教授 (70398973)

Co-Investigator(Kenkyū-buntansha) 吉田 学  東京大学, 大学院理学系研究科(理学部), 准教授 (60301785)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords精子 / 糖衣 / 精嚢分泌タンパク質 / 卵管 / 受精能獲得 / 精嚢タンパク質 / グリコカリックス / ステロール
Outline of Research at the Start

DEFB126のマウスにおける相同タンパク質はβディフェンシン-22(DEFB22)であり、同様に精子膜表面最外層にグリコカリックスを形成することが示されている。しかしながら、これらグリコカリックスと精嚢分泌タンパク質の相互作用については明らかになっていない。そこで、本研究ではマウスを用い、まず、1)SVS2とDEFB22の相互作用を明らかにする。次
に、2)SVS2およびDEFB22の精子膜からの除去機構をin vivoで検討する。2)で明らかになった機構を手がかりに、3)精子膜ステロールレベル調節について、関わる細胞内外の因子を探索し、同定する。

Outline of Final Research Achievements

Although the efflux of cholesterol from the sperm membrane is triggered during the capacitation, the physiological regulatory mechanism is unknown. We have previously shown that seminal vesicle secretory proteins actively maintain cholesterol levels in the sperm membrane. We hypothesize that this action suppresses ectopic fertilization potential acquisition, but the molecular mechanism is not clear. In this study, mouse utero-tubal junction (UTJ) was cultured for a short time and tissue transparency enabled observation of sperm binding to the oviductal epithelium. In addition, lectin arrays of sperm cell membrane extracts were performed, and it was shown that the sperm cell membrane structure of glycan chains is altered in conjunction with the removal of cholesterol by MbCD and the regulation of the process of fertilization ability acquisition by the presence of sperm seminal vesicle secretory protein SVS2.

Academic Significance and Societal Importance of the Research Achievements

我々を含むほ乳類に共通の体内受精の仕組みを理解することは、挙児を希望するカップルに安全かつ有効な生殖補助医療を提供する基礎となる。特に、本研究のこれまで顧みられることの少なかった精漿成分の体内受精における生理的な役割を明らかにする試みは生殖補助医療への応用も期待できる重要な知見である。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (4 results)

All 2021 2020 2019

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results)

  • [Journal Article] Identification of an antibacterial polypeptide in mouse seminal vesicle secretions2021

    • Author(s)
      Morohoshi Kazunori、Yamazaki Takeo、Kito Keiji、Sato Ban、Kang Woojin、Hibino Taku、Yoshida Manabu、Yoshida Kaoru、Iwamoto Teruaki、Yamada Mitsutoshi、Miyado Kenji、Kawano Natsuko
    • Journal Title

      Journal of Reproductive Immunology

      Volume: 148 Pages: 103436-103436

    • DOI

      10.1016/j.jri.2021.103436

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Human Semenogelin 1 Promotes Sperm Survival in the Mouse Female Reproductive Tract.2020

    • Author(s)
      Sakaguchi D, Miyado K, Iwamoto T, Okada H, Yoshida K, Kang W, Suzuki M, Yoshida M, Kawano N
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 11 Pages: 3961-3961

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Deletion of a seminal gene cluster reinforces a crucial role of SVS2 in male fertility.2019

    • Author(s)
      Shindo, M., Inui, M., Kang, W., Tamano, M., Tingwei, C., Takada, S., Hibino, T., Yoshida, M., Yoshida, K., Okada, H., Iwamoto, T., Miyado, K., and Kawano, N.
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20(18) Pages: 4557-4557

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 受精時に見られる精子運動調節2019

    • Author(s)
      吉田 薫,吉田 学
    • Journal Title

      細胞

      Volume: 51(5) Pages: 253-255

    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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