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Establishment of a new strategy for the treatment of functional restoration in skeletal muscle injury by tissue engineering using osteopontine derived SVVYGLR peptide

Research Project

Project/Area Number 19K10265
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57060:Surgical dentistry-related
Research InstitutionOsaka University

Principal Investigator

Tanaka Susumu  大阪大学, 歯学研究科, 教授 (00367541)

Co-Investigator(Kenkyū-buntansha) 濱田 吉之輔  大阪大学, 医学系研究科, 特任准教授 (10362683)
河口 直正  森ノ宮医療大学, 保健医療学研究科, 教授 (70224748)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords骨格筋 / オステオポンチン / SVVYGLR / 筋機能再生 / SVVYGLRペプチド / 再生医療
Outline of Research at the Start

本研究では、骨格筋外傷・手術後の重度の筋断裂、損傷、瘢痕線維化による運動機能低下に対して、SVペプチドが骨格筋の組織再生ならびに機能再生を促進しうるかを明らかにする目的で、①骨格筋(咀嚼筋、軟口蓋筋)損傷モデルを用いてSVペプチドが筋活動(摂食運動・鼻咽腔閉鎖運動)特性に及ぼす影響について生理学的に検討・解析するとともに、②骨格筋前駆細胞(筋衛星細胞・筋芽細胞)を用いて、細胞生物学的特性(増殖・分化能)に対するSVペプチドの作用効果についても分子生物学的に明らかとしていく。本研究成果をもとにペプチド製剤による新規骨格筋再生治療法の確立を目指す。

Outline of Final Research Achievements

Severe skeletal muscle injury by trauma or surgery could cause repair with excessive scar tissue and leads to persistent functional impairment. This project aimed to investigate the effects of osteopontin-derived synthetic peptide SVVYGLR (SV) on the biological properties of myogenic precursor cells and functional regeneration of injured skeletal muscle using volumetric muscle loss animal models.
Synthetic SV peptide showed no influence on the proliferation property of human-derived satellite cells and myoblasts, while cell migratory activity of those cells was significantly increased. In addition, local administration of SV peptide in animal models revealed the facilitation of regeneration of muscle tissue with matured myofibers and scarless healing, which contributed to favorable functional recovery after muscle injury.These results suggest that SV peptide could be a powerful alternative option as a new regenerative treatment for severe muscle injury.

Academic Significance and Societal Importance of the Research Achievements

骨格筋は、運動や軽微な損傷に対しては、速やかな修復、再生が行われるが、外傷、手術による筋組織の重篤な損傷や発育不全を有する先天性疾患においては、従来の治療、形成手術を行ったとしても十分な機能回復、機能獲得が得られないケースも少なくない。筋肉の再生医療について、骨格筋を対象とした再生医療技術として臨床応用されているものは未だ存在しない。本研究成果より、生体由来の機能性ペプチドである本被検物を損傷骨格筋に局所投与することで、筋組織の再生を促進し、良好な機能再生をもたらすことが明らかとなり、新たな骨格筋の機能再生治療法となる可能性が示唆された。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (7 results)

All 2021 2020

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] The synthetic peptide SVVYGLR promotes myogenic cell motility <i>via</i> the TGFβ1/Smad signaling pathway and facilitates skeletal myogenic differentiation <i>in vitro </i>2021

    • Author(s)
      Hamada Y, Tanaka S, Fujishita Y, Cho J-S, Usuki T, Yokoyama Y, Wu X, Mori S, Yamamoto H, Kogo M.
    • Journal Title

      Dental Materials Journal

      Volume: 40 Issue: 4 Pages: 957-963

    • DOI

      10.4012/dmj.2020-354

    • NAID

      130008069814

    • ISSN
      0287-4547, 1881-1361
    • Year and Date
      2021-07-25
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Osteopontin-derived synthetic peptide SVVYGLR upregulates functional regeneration of oral and maxillofacial soft-tissue injury2021

    • Author(s)
      Tanaka S, Hamada Y, Yokoyama Y, Yamamoto H, Kogo M
    • Journal Title

      Japanese Dental Science Review

      Volume: 57 Pages: 174-181

    • DOI

      10.1016/j.jdsr.2021.09.002

    • NAID

      210000172918

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The synthetic peptide SVVYGLR promotes cell motility of myogenic cells and facilitates differentiation in skeletal muscle regeneration2021

    • Author(s)
      Tanaka S, Fujishita Y, Kawaguchi N, Usuki T, Yokoyama Y, Wu X, Mori S, Yamamoto H, Kogo M.
    • Journal Title

      Dental Materials Journal

      Volume: advpub Issue: 0 Pages: 317-317

    • DOI

      10.4012/dmj.2020-317

    • NAID

      130008045523

    • ISSN
      0287-4547, 1881-1361
    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synthetic peptide SVVYGLR upregulates cell motility and facilitates oral mucosal wound healing.2020

    • Author(s)
      Tanaka S, Yasuda T, Hamada Y, Kawaguchi N, Fujishita Y, Mori S, Yokoyama Y, Yamamoto H, Kogo M.
    • Journal Title

      Peptides

      Volume: 134 Pages: 170405-170405

    • DOI

      10.1016/j.peptides.2020.170405

    • Related Report
      2020 Research-status Report
    • Peer Reviewed
  • [Presentation] 機能性ペプチドSVVYGLRの複数回投与が損傷骨格筋の創傷治癒に及ぼす作用2021

    • Author(s)
      趙正秀,田中晋,藤下陽平,古郷幹彦
    • Organizer
      第75回日本口腔科学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 機能性ペプチドSVVYGLRが口蓋損傷後の筋機能再生と顎発育に及ぼす作用2021

    • Author(s)
      趙正秀,田中晋,島岡祐加子,古郷幹彦
    • Organizer
      第45回日本口蓋裂学会総会・学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 機能性ペプチドSVVYGLRによる損傷骨格筋の修復ならびに筋機能再生治療法の開発2020

    • Author(s)
      田中晋,藤下陽平,趙 正秀,安田卓司, 松下豊,薄木崇介,島岡祐加子,古郷幹彦
    • Organizer
      第65回日本口腔外科学会総会・学術集会
    • Related Report
      2020 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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