Does modulation of muscle function in nasal obstruction during growth affect maxillofacial development?

• Project/Area Number: 19K10377
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 57070:Developmental dentistry-related
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Kokai Satoshi 東京医科歯科大学, 大学院医歯学総合研究科, 非常勤講師 (50431937)
• Co-Investigator(Kenkyū-buntansha): 渡 一平 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (10431941) ; 小野 卓史 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (30221857) ; 加藤 千帆 東京医科歯科大学, 歯学部附属病院, 特任助教 (80706987)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2021: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000); Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 鼻呼吸障害 / 成長期 / 咀嚼野 / 大脳 / 鼻閉 / 睡眠時無呼吸 / 筋機能訓練 / 睡眠時無呼吸 筋機能訓練 / 顔面発育 / 筋機能

Outline of Research at the Start

本研究では顎顔面発育形成段階である成長期における鼻呼吸障害が、顎口腔周囲筋の筋活動を変調し、筋機能低下をもたらすメカニズムを、遅筋速筋の割合およびエピジェネティクスの代表的な客観的指標であるヒストン修飾分布量、速筋または遅筋特異的な遺伝子発現量の変化を指標として解析し、筋活動の変化が顎顔面形態へ反映されるメカニズムを解明していくことを目的としている。

Outline of Final Research Achievements

The purpose of this study was to elucidate the effects of chronic nasal breathing disorders during growth on the development of the masticatory area of the cerebral cortex, which plays an important role in masticatory movements, using intracortical microelectrical stimulation. The results of the measurement of jaw movement by intracortical microelectrical stimulation of the cortical masticatory area using a high-speed camera revealed that nasal breathing disorder during growth affects the development of maxillofacial motor representations in the same area.

Academic Significance and Societal Importance of the Research Achievements

矯正歯科臨床において、鼻閉や口呼吸を有する者が多く見受けられる。大脳皮質咀嚼野は咀嚼運動の制御に関与する高次機能領域である。本研究は、SpO 2 レベルと大脳皮質咀嚼野に着目し、成長期における鼻呼吸障害が末梢における変化のみならず、咀嚼運動の中枢神経系である大脳皮質咀嚼野の発達に与える影響について初めて明らかにした。今後の歯科臨床のみならず、歯科医学や神経生理学の発展に寄与するところが極めて大きいと考えられる。

Research Products

• [Journal Article] Histological and contractile changes in the genioglossus muscle after nasal obstruction in growing rats (2023)
  • Author(s): Koecklin Karin Harumi Uchima、Kato Chiho、Abe Yasunori、Yabushita Tadachika、Kokai Satoshi、Ono Takashi
  • Journal Title: Scientific Reports Volume: 13 Issue: 1
  • DOI: 10.1038/s41598-023-32921-w
  • Peer Reviewed / Open Access
• [Journal Article] Unilateral nasal obstruction affects development of cortical orofacial motor representation in the cortical masticatory area of growing rats (2022)
  • Author(s): Fujita Akiyo、Kato Chiho、Abe Yasunori、Ishidori Hideyuki、Li Ruixin、Thura Aung Phyo、Ogawa Takuya、Okihara Hidemasa、Kokai Satoshi、Ono Takashi
  • Journal Title: Neuroscience Letters Volume: 783 Pages: 136700-136700
  • DOI: 10.1016/j.neulet.2022.136700
  • Peer Reviewed
• [Journal Article] Nasal obstruction during the growth period modulates the Wnt/β‐catenin pathway and brain‐derived neurotrophic factor production in association with tyrosine kinase receptor B mRNA reduction in mouse hippocampus (2021)
  • Author(s): Ishidori Hideyuki、Okihara Hidemasa、Ogawa Takuya、Abe Yasunori、Kato Chiho、Aung Phyo Thura、Fujita Akiyo、Kokai Satoshi、Ono Takashi
  • Journal Title: European Journal of Neuroscience Volume: 55 Issue: 1 Pages: 5-17
  • DOI: 10.1111/ejn.15547
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional Analysis of Rhythmic Jaw Movements Evoked by Electrical Stimulation of the Cortical Masticatory Area During Low Occlusal Loading in Growing Rats (2020)
  • Author(s): Aung Phyo Thura、Kato Chiho、Abe Yasunori、Ogawa Takuya、Ishidori Hideyuki、Fujita Akiyo、Okihara Hidemasa、Kokai Satoshi、Ono Takashi
  • Journal Title: Frontiers in Physiology Volume: 11 Pages: 1-16
  • DOI: 10.3389/fphys.2020.00034
  • Peer Reviewed / Open Access
• [Presentation] Effects of unilateral nasal obstruction during the growth period on cerebellar neural circuit and function (2023)
  • Author(s): Moe Tanigawa, Chiho Kato, Takashi Ono, Naofumi Uesaka
  • Organizer: The 100th Anniversary Annual Meeting of The Physiological Society of Japan,
• [Presentation] Altered neuromuscular behavior during cortically-elicited rhythmic jaw movement in obese Zucker rat (2022)
  • Author(s): Li Ruixin, Kato Chiho, Abe Yasunori, Fujita Akiyo, Ogawa Takuya, Ishidori Hideyuki, Misawa Eri, Okihara Hidemasa, Kokai Satoshi, Ono Takashi
  • Organizer: 第81回日本矯正歯科学会
• [Presentation] Impact of change in food properties on learning/memory function in the growing mice (2022)
  • Author(s): Sun Wenqian, Ogawa Takuya, Okihara Hidemasa, Ishidori Hideyuki, Misawa Eri, Kato Chiho, Ono Takashi
  • Organizer: 第81回日本矯正歯科学会
• [Presentation] 成長期咀嚼刺激低下はWnt signaling pathwayおよびARHGAP33の分子伝達を変調する (2021)
  • Author(s): 三澤 英里, 沖原 秀政, 小川 卓也, 小海 暁, 阿部 泰典, 加藤 千帆, 石通 秀行, 藤田 秋世, 高野 力磨, 谷川 萌, 孫 文倩, 小野 卓史
  • Organizer: 第80回日本矯正歯科学会大会
• [Presentation] Unilateral nasal obstruction affects development of motor representation in the cortical masticatory area in growing rats (2020)
  • Author(s): Akiyo Fujita, Chiho Kato, Yasunori Abe, Takuya Ogawa, Phyo Thura Aung, Hideyuki Ishidori, Ruixin Li, Hidemasa Okihara, Satoshi Kokai, Takashi Ono
  • Organizer: The 9th international Orthodontic Congress
  • Int'l Joint Research
• [Presentation] Low occlusal loading affects the neuromuscular control of jaw movements induced by anterior part of cortical masticatory area in growing rats (2020)
  • Author(s): Phyo Thura Aung, Chiho Kato, Yasunori Abe, Takuya Ogawa, Hideyuki Ishidori, Akiyo Fujita, Ruixin Li, Hidemasa Okihara, Satoshi Kokai, Takashi Ono
  • Organizer: The 9th international Orthodontic Congress
  • Int'l Joint Research
• [Presentation] マウス成長期鼻呼吸障害は海馬におけるWnt signaling pathwayに変調をもたらす (2019)
  • Author(s): 石通 秀行, 沖原 秀政, 小川 卓也, 小海 暁, 阿部 泰典, 加藤 千帆, ピョースラ・アン , 藤田 秋世, 小野 卓史
  • Organizer: 第78回 日本矯正歯科学会大会