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A Study on amoebic dynamics and interactions in Legionella species for risk assessment of Legionella infection.

Research Project

Project/Area Number 19K12376
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 64010:Environmental load and risk assessment-related
Research InstitutionKobe Institute of Health

Principal Investigator

Nakanishi Noriko  神戸市健康科学研究所, 感染症部, 副部長 (50615490)

Co-Investigator(Kenkyū-buntansha) 野本 竜平  神戸市健康科学研究所, その他部局等, 副部長 (60642238)
Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsレジオネラ属菌 / 宿主アメーバ / 感染 / 相互作用 / アメーバ内動態 / ゲノム解析 / RNA-seq / L.pneumophila / アメーバ類 / 増殖能 / アメーバ / 全ゲノム解析 / RNA-Seq / 比較ゲノム / 菌種間相互作用 / 比較ゲノム解析
Outline of Research at the Start

Legionella pneumophila以外のレジオネラ属菌の環境中の挙動は不明な点が多い。本研究では、アメーバ内のレジオネラ属菌種間の相互作用に着目し、種々のアメーバを用いた感染実験により生存能や増殖能を解析し、オミクス解析によるアメーバ内のレジオネラ属菌の挙動を分子レベルで明らかにする。さらに、ゲノムワイドな多株比較解析からレジオネラ属菌種間のゲノム上の可動性遺伝領域の構造比較や組換えの実態を明らかにすることで、生活環境下に存在するレジオネラ属菌が高病原性になるリスクを評価する。

Outline of Final Research Achievements

The purpose of this study was to elucidate the interactions between host amoeba and Legionella species in various environments. We found differences in infectivity between Legionella species in infection experiment using various amoebae. The difference in the growth rate of L. pneumophila in some amoebae was comprehensively analyzed by RNA-seq for the variation in gene expression in the presence of amoebae, suggesting that it may be due to the difference in gene expression after internalinvasion within the amoebae. Furthermore, the plasmids possessed by L. pneumophila that colonize cooling towers may contribute to the pathogenicity and adaptation to the cooling tower environment.

Academic Significance and Societal Importance of the Research Achievements

本研究の成果は、環境中でレジオネラが生残していく戦略を明らかにすると共に、それに必要な因子をマーカーとすることで、環境中におけるレジオネラ感染制御法が確立されることが期待される。また、レジオネラ属菌種の病原性獲得機構および生存戦略の解明は、感染リスクの評価や公衆衛生の観点からも重要である。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (14 results)

All 2023 2022 2021 2020 2019

All Journal Article (6 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Evaluation of <i>Legionella pneumophila</i> SGUT Serotypes Isolated from Bath Water Using a Multiplex-PCR Serotyping Assay2023

    • Author(s)
      Komatsu Shoko、Tanaka Shinobu、Nakanishi Noriko
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 76 Issue: 1 Pages: 77-79

    • DOI

      10.7883/yoken.JJID.2022.397

    • ISSN
      1344-6304, 1884-2836
    • Year and Date
      2023-01-31
    • Related Report
      2023 Annual Research Report 2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Prevalence and genetic distribution of Legionella spp. in public bath facilities in Kobe City, Japan2023

    • Author(s)
      Komatsu Shoko、Tanaka Shinobu、Nakanishi Noriko
    • Journal Title

      Journal of Water and Health

      Volume: 21 Issue: 11 Pages: 1727-1734

    • DOI

      10.2166/wh.2023.247

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 市内浴場施設における理化学検査とレジオネラ属菌検出との関連性について2023

    • Author(s)
      藤永千波、小松頌子、田中忍、八木正博、中西典子、大久保祥嗣、向井健悟
    • Journal Title

      神戸市健康科学研究所報

      Volume: 51 Pages: 58-61

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Investigation of a Legionella pneumophila Outbreak at a Bath Facility in Japan Using Whole-Genome Sequencing of Isolates from Clinical and Environmental Samples2022

    • Author(s)
      Nakanishi Noriko、Komatsu Shoko、Tanaka Shinobu、Mukai Kengo、Nomoto Ryohei
    • Journal Title

      Microorganisms

      Volume: 11 Issue: 1 Pages: 28-28

    • DOI

      10.3390/microorganisms11010028

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 市内温泉施設における緊急事態宣言後のレジオネラ属菌の検出状況と遺伝子型の推移2021

    • Author(s)
      小松頌子 、中西典子、 岩本朋忠
    • Journal Title

      神戸市健康科学研究所報

      Volume: 第 49 巻 Pages: 39-42

    • Related Report
      2021 Research-status Report
  • [Journal Article] 人工環境水におけるアメーバ類の生息実態とレジオネラ属菌の関連性2020

    • Author(s)
      田中忍、中西典子、岩本朋忠
    • Journal Title

      神戸市環境保健研究所 所報

      Volume: 第48巻 Pages: 51-55

    • Related Report
      2020 Research-status Report
  • [Presentation] 公衆浴場から分離されたレジオネラ属菌の生態学的・遺伝的特徴(2016~2021)2023

    • Author(s)
      小松頌子 、田中忍、中西典子
    • Organizer
      日本防菌防黴学会第50回年次大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] The genetic characterization of L. pneumophila SG1 isolates from bath water in Kobe City, Japan.2022

    • Author(s)
      Shoko Komatsu, Shinobu Tanaka, Ryohei Nomoto, Noriko Nakanishi
    • Organizer
      The 10th International Conference on Legionella.
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Whole-Genome analysis of L. pneumophila strains causing outbreak at bath facility in Kobe, Japan.2022

    • Author(s)
      Noriko Nakanishi, Shoko Komatsu, Shinobu Tanaka, Ryohei Nomoto.
    • Organizer
      The 10th International Conference on Legionella.
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Bacterial counts by flow cytometry can determine presence/absence of Legionella in bath water.2022

    • Author(s)
      Taguri, T., Cai, G., Nakanishi, N., Hiratsuka, T., Inoue, H., Shimoda, T., Shinmichi K., Kura F. and Amemura-Maekawa, J
    • Organizer
      The 10th International Conference on Legionella.
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Acquisition of antimicrobial-resistant variants in repeated infections caused by Pseudomonas aeruginosa2020

    • Author(s)
      Kayo Osawa, Noriko Nakanishi, Mari Kusuki, Katsumi Shigemura, Takayuki Miyara:
    • Organizer
      第94回日本感染症学会総会・学術講演会
    • Related Report
      2020 Research-status Report
  • [Presentation] 冷却塔に定着するL. pneumophilaのゲノム分子疫学解析2020

    • Author(s)
      中西典子・野本竜平・田中忍・有川健太郎・岩本朋忠
    • Organizer
      第13回日本ゲノム微生物学会年会
    • Related Report
      2019 Research-status Report
  • [Presentation] 人工環境水から検出されたアメーバ類とレジオネラ属菌との関連性2019

    • Author(s)
      田中忍、中西典子、岩本朋忠
    • Organizer
      令和元年度(第46回) 地方衛生研究所全国協議会近畿支部 細菌部会研究会
    • Related Report
      2019 Research-status Report
  • [Presentation] Monitoring and Distribution of Legionella in artificially made water system in Kobe City, Japan2019

    • Author(s)
      Noriko Nakanishi
    • Organizer
      The 60th Anniversary of School of Public Health (SPH), Fujian Medical University (FJMU), A joint symposium on public health and environment in southeastern China and Ja
    • Related Report
      2019 Research-status Report
    • Invited

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Published: 2019-04-18   Modified: 2025-01-30  

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