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Investigation of lipid production characteristics of thraustochytrids and application for accelerating transformation of food waste to eco-feed

Research Project

Project/Area Number 19K12398
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 64030:Environmental materials and recycle technology-related
Research InstitutionHiroshima University

Principal Investigator

Nakai Satoshi  広島大学, 先進理工系科学研究科(工), 教授 (80313295)

Co-Investigator(Kenkyū-buntansha) 西嶋 渉  広島大学, 環境安全センター, 教授 (20243602)
新居 隆浩  広島大学, 統合生命科学研究科(生), 助教 (90804873)
Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsスラウストキトリッド / DHA / EPA / 食品残渣 / エコフィード / 家禽製品 / ドコサヘキサエン酸 / 食品廃棄物 / 産卵鶏 / 鶏肉 / 高度不飽和脂肪酸 / 植物性食品残渣
Outline of Research at the Start

本研究では、植物性食品残渣を用いてドコサヘキサエン酸(DHA)などの有用物質を生産するスラウストキトリッドを培養し、食品残渣と同微生物バイオマスの混合物を、家禽生産物を高価値化させる飼料として利用する手法を確立する。まず、同微生物の炭素源、窒素源の要求性と共に、基質や培養条件が脂質生産量や組成に与える影響とその変遷機構も明らかする。さらに、脂質生産と養鶏に好適な植物性食品残渣を把握し、同微生物の増殖特性を利用して滅菌を伴わない簡便な培養法を見出す。そして、植物性食品残渣と各株バイオマスの混合物をニワトリに給餌し、産卵機能と鶏卵の有用脂質の蓄積量を評価し、混合物の給餌効果を実証する。

Outline of Final Research Achievements

The objectives of this study was to establish methodologies to cultivate thraustochytrids that produce docosahexaenoic and eicosapentaenoic acids (DHA and EPA) using food residues and to use the resultant mixture of thraustochytrid biomass and food residues as a functional eco-feed giving high values to poultry products via strengthening DHA and EPA contents. First, the lipid production characteristics of the thraustochytrids were investigated. Next, the thraustochytrids were cultivated using the food residues to obtain a mixture of food residues and thraustochytrid biomass, and the mixture was fed to poultry. The results confirmed that feeding of the biomass mixture increased the DHA and EPA content in chicken eggs and the DHA content in thighs and breast meat.

Academic Significance and Societal Importance of the Research Achievements

本研究では、DHAやEPAを生産するスラウストキトリッドという微生物を食品残渣を用いて培養し、結果として得られるバイオマス混合物を、鶏卵や鶏肉といった家禽製品に含まれるDHAやEPAの含有量を増加させられる、家禽飼料のサプリメントとして利用する手法について検討した。実際にバイオマス混合物を産卵鶏に与えた結果、鶏卵中のDHAやEPA、もも肉やむね肉といった鶏肉中のDHAが増加することを確認した。このように、本研究では食品残渣の再利用促進と価値の高い家禽製品の生産を両立させられる成果を得た。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (9 results)

All 2022 2021 2020 2019 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results)

  • [Int'l Joint Research] Institut Teknologi Sepuluh Nopember(インドネシア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] Institut Teknologi Sepuluh Nopember(インドネシア)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Effects of Dilution on Removal of Organic Carbon and Nitrogen From Miso-production Wastewater by <i>Aurantiochytrium</i> sp. L3W and Resultant Fatty Acid Production2021

    • Author(s)
      Nurlaili Humaidah, Satoshi Nakai, Wataru Nishijima, Takehiko Gotoh
    • Journal Title

      ENVIRONMENTAL SCIENCE

      Volume: 34 Issue: 1 Pages: 18-26

    • DOI

      10.11353/sesj.34.18

    • NAID

      130007978309

    • ISSN
      0915-0048, 1884-5029
    • Year and Date
      2021-01-31
    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 炭素循環による持続的な食料生産への一歩2022

    • Author(s)
      中井智司
    • Organizer
      第34回環境工学連合講演会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 食品廃棄物を用いて培養したAurantiochytrium sp. バイオマスの給餌によるDHA強化家禽産物の生産2021

    • Author(s)
      浅尾 航、中井 智司、西嶋 渉、後藤 健彦、新居 隆浩
    • Organizer
      化学工学会 第52回秋季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Production of Eco-feed using Aurantiochytrium sp. and its application for poultry breeding2021

    • Author(s)
      Asao W, Nakai S, Nishijima W, Gotoh T, Nii T
    • Organizer
      Water and Environment Technology Conference Online2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Aurantiochytrium sp. L3W株を用いた植物性残渣の高機能エコフィード化2020

    • Author(s)
      浅尾 航・中井 智司・西嶋 渉・後藤 健彦・新居 隆浩
    • Organizer
      化工学会 第51回秋季大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Production of Fish Feed Additive from Food Wastes Using2019

    • Author(s)
      Megumi FURUTA, Satoshi NAKAI, Nurlaili HUMAIDAH, Wataru NISHIJIMA, Takehiko GOTOH
    • Organizer
      Water and Environment Technology Conference 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] A novel strain of Aurantiochytrium sp. and its growth characteristics usable for cultivation in food industry wastewater without sterilization toward to production of polyunsaturated fatty acids2019

    • Author(s)
      Satoshi Nakai, Nurlaili Humaidah, Takehiko Gotoh, Wataru Nishijima, Tetsuji Okuda, Masaki Ohno
    • Organizer
      MESCYT 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2019-04-18   Modified: 2023-12-25  

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