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Analysis of oxidoreductases directly selected from wood decay environments

Research Project

Project/Area Number 19K15881
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 40020:Wood science-related
Research InstitutionHokkaido University

Principal Investigator

Hori Chiaki  北海道大学, 工学研究院, 助教 (50722948)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Discontinued (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2020: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords木材腐朽 / バイオマス / 木材分解酵素 / 植物細胞壁 / 木材腐朽菌
Outline of Research at the Start

これまでの研究では、植物成分の分解に関わる酵素(セルラーゼ等)について、単離された微生物から一つずつ生化学的解析を進めることで詳細な分子機能が明らかになってきた。しかし、環境下において様々な微生物が存在する場合の植物分解についてはほとんど知見が得られていなかった。そこで、本研究では実際に自然環境下で選抜された酵素について生化学的解析を行い、より効率的な植物分解メカニズムを明らかにすることを目標としている。このような知見は植物バイオマス成分を分解する糖化技術を効率化することに繋がると考えている。

Outline of Final Research Achievements

Molecular functions of enzymes involved in the decomposition of plant components such as cellulases and hemicellulases have been elucidated by using biochemical analyses on enzymes from isolated microorganisms. However, little knowledge has been revealed about plant decomposition by various microorganisms in the natural environment. Therefore, in this study, we try to clarify plant decomposition mechanisms by conducting biochemical analysis on the enzymes in the natural environment.

Academic Significance and Societal Importance of the Research Achievements

植物細胞壁の主要な高分子成分(セルロース・ヘミセルロース・リグニン)の分解酵素については、腐朽木材から単離された菌を試験管内で培養・単離・解析することで検証されてきた。一方で環境中で実際に働いている酵素についての理解は不十分であった。そこで本研究では、これら自然環境下での木材分解酵素を対象として、酵素を生化学的に解析し、多様な微生物が関与する分解酵素の一部を明らかにした。

Report

(3 results)
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Research-status Report
  • Research Products

    (33 results)

All 2021 2020 2019 Other

All Int'l Joint Research (2 results) Journal Article (10 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 10 results,  Open Access: 6 results) Presentation (20 results) (of which Int'l Joint Research: 8 results,  Invited: 6 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] Forest Products Laboratory/JGI institute(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] USDA Forest Products Laboratory(米国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Impact of abiotic stress on the regulation of cell wall biosynthesis in <i>Populus trichocarpa</i>2020

    • Author(s)
      Hori Chiaki、Yu Xiang、Mortimer Jenny C.、Sano Ryosuke、Matsumoto Tomoko、Kikuchi Jun、Demura Taku、Ohtani Misato
    • Journal Title

      Plant Biotechnology

      Volume: 37 Issue: 3 Pages: 273-283

    • DOI

      10.5511/plantbiotechnology.20.0326a

    • NAID

      130007907472

    • ISSN
      1342-4580, 1347-6114
    • Year and Date
      2020-09-25
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identifying transcription factors that reduce wood recalcitrance and improve enzymatic degradation of xylem cell wall in Populus2020

    • Author(s)
      Hori Chiaki、Takata Naoki、Lam Pui Ying、Tobimatsu Yuki、Nagano Soichiro、Mortimer Jenny C.、Cullen Dan
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 22043-22043

    • DOI

      10.1038/s41598-020-78781-6

    • NAID

      120007189282

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transport-coupled ubiquitination of the borate transporter BOR1 for its boron-dependent degradation2020

    • Author(s)
      A. Yoshinari, T. Hosokawa, M. P. Beier, K. Oshima, Y. Ogino, C. Hori, T. E. Takasuka, Y. Fukao, T. Fujiwara and J. Takano
    • Journal Title

      Plant Cell

      Volume: 33(2) Issue: 2 Pages: 420-438

    • DOI

      10.1093/plcell/koaa020

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Isolation of poly[d-lactate (LA)-co-3-hydroxybutyrate)]-degrading bacteria from soil and characterization of d-LA homo-oligomer degradation by the isolated strains2020

    • Author(s)
      Chiaki Hori, Tomohiro Sugiyama, Kodai Watanabe, Jian Sun,Yuu Kamada Toshihiko Ooi, Takuya Isono,Toshifumi Satoh, Shin-ichiro Sato, Seiichi Taguchi, Ken'ichiro Matsumoto
    • Journal Title

      Polymer Degradation and Stability

      Volume: 179 Pages: 109231-109231

    • DOI

      10.1016/j.polymdegradstab.2020.109231

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Proteomic Characterization of Lignocellulolytic Enzymes Secreted by the Insect-Associated Fungus Daldinia decipiens oita, Isolated from a Forest in Northern Japan2020

    • Author(s)
      Hori Chiaki、Song Ruopu、Matsumoto Kazuki、Matsumoto Ruy、Minkoff Benjamin B.、Oita Shuzo、Hara Hideho、Takasuka Taichi E.
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 86 Issue: 8

    • DOI

      10.1128/aem.02350-19

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Synergy of valine and threonine supplementation on poly(2-hydroxybutyrate-block-3-hydroxybutyrate) synthesis in engineered Escherichia coli expressing chimeric polyhydroxyalkanoate synthase2020

    • Author(s)
      Sudo Maho、Hori Chiaki、Ooi Toshihiko、Mizuno Shoji、Tsuge Takeharu、Matsumoto Ken'ichiro
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 129 Issue: 3 Pages: 302-306

    • DOI

      10.1016/j.jbiosc.2019.09.018

    • NAID

      120006980040

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Origin and Diversity of Wood Decay Fungi Revealed by Genome-Based Analyses2019

    • Author(s)
      Hori Chiaki、Yoshida Makoto、Igarashi Kiyohiko、Samejima Masahiro
    • Journal Title

      Mokuzai Gakkaishi

      Volume: 65 Issue: 4 Pages: 173-188

    • DOI

      10.2488/jwrs.65.173

    • NAID

      130007741345

    • ISSN
      0021-4795, 1880-7577
    • Year and Date
      2019-10-25
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Genome sequence of Striga asiatica provides insight into the evolution of plant parasitism.2019

    • Author(s)
      Yoshida S. et al.
    • Journal Title

      Current Biology

      Volume: 29 Issue: 18 Pages: 3041-3052

    • DOI

      10.1016/j.cub.2019.07.086

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ribulose-1,5-bisphosphate carboxylase/oxidase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli.2019

    • Author(s)
      Matsumoto K, Saito J, Yokoo T, Hori C, Nagata A, Kudoh Y, Ooi T, Taguchi S.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 印刷中 Issue: 3 Pages: 302-306

    • DOI

      10.1016/j.jbiosc.2019.03.002

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] The Structural Integrity of Lignin Is Crucial for Resistance against Striga hermonthica Parasitism in Rice2019

    • Author(s)
      Mutuku J. Musembi、Cui Songkui、Hori Chiaki、Takeda Yuri、Tobimatsu Yuki、Nakabayashi Ryo、Mori Tetsuya、Saito Kazuki、Demura Taku、Umezawa Toshiaki、Yoshida Satoko、Shirasu Ken
    • Journal Title

      Plant Physiology

      Volume: 179 Issue: 4 Pages: 1796-1809

    • DOI

      10.1104/pp.18.01133

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 植物バイオマス分解利用に関する基礎研究2021

    • Author(s)
      堀千明
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 微生物による木材腐朽の多様性に関するオミクス解析2021

    • Author(s)
      堀千明
    • Organizer
      第71回 日本木材学会大会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] RuBisCO経路を利用した組換え大腸菌によるポリヒドロキシアルカン酸の合成とRuBisCO評価系への応用2021

    • Author(s)
      藤原悠暉、斎藤樹理、永田暁洋、大潟祥梧、二宮太樹、谷口遼、松本直己、堀千明、大井俊彦、蘆田弘樹、松本謙一郎
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Aspergillus nidulans由来新規ラムのガラクツロナンリアーゼの機能解析2021

    • Author(s)
      伊藤昴希、鈴木裕満、鈴木健吾、酒井杏匠、高須賀太一、堀千明、加藤雅士、志水元亨
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 病原性腐朽菌ベッコウタケ 由来ラッカーゼ と樹木由来生体防御物質との反応2020

    • Author(s)
      久保田美穂、堀千明、大井俊彦、松本謙一郎
    • Organizer
      糸状菌分子生物学カンファレンス若手の会2020年度大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 微生物による木材腐朽の多様性に関するオミクス解析2019

    • Author(s)
      堀千明
    • Organizer
      セルロース学会北海道・東北支部セミナー
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Meta-multi omics analyses of wood decay in nature.2019

    • Author(s)
      Hori C
    • Organizer
      Asian Micology Congress 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 担子菌によるバイオマス分解のメカニズム2019

    • Author(s)
      堀千明
    • Organizer
      第33回セルラーゼ研究会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Microbial strategy for wood degradation in nature ecosystem2019

    • Author(s)
      Hori C
    • Organizer
      International Symposium on Biopolymer Synthesis and Degradation
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 木材腐朽菌による植物分解の多様性と進化2019

    • Author(s)
      堀千明
    • Organizer
      第63回日本菌学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Enzymatic mechanism of tolerance to tree defense substrates by the pathogen.2019

    • Author(s)
      Kubota M, Matsumoto R, Hori C, Ooi T, Matsumoto K
    • Organizer
      Hokkaido University-National Central University Joint Symposium on Material Chemistry and Physics 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular mechanism of detoxifying plant defense substrates by wood decay fungi.2019

    • Author(s)
      Matsumoto R, Hori C, Ooi T, Matsumoto K
    • Organizer
      ICBP2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular mechanism of detoxifying plant defense substrates by wood pathogens2019

    • Author(s)
      Matsumoto R, Hori C, Ooi T, Matsumoto K
    • Organizer
      International Symposium on Biopolymer Synthesis and Degradation
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] The mechanism of conifer wood degradation by white-rot fungus, Phlebiopsis gigantea2019

    • Author(s)
      Iwata M, Hori C, Ooi T, Matsumoto K
    • Organizer
      International Symposium on Biopolymer Synthesis and Degradation
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] In vitro analysis of D-lactyl-CoA-polymerizing enzyme in lactate-containing polyhydroxyalkanoates synthesis2019

    • Author(s)
      Arai S, Iijima M, Hori C, Utsunomia C, Ooi T, Taguchi S, Matsumoto K
    • Organizer
      International Symposium on Biopolymer Synthesis and Degradation
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] In vitro evolution of polyester synthase for enhanced production of 2-hydroxybutyrate-containing polymer2019

    • Author(s)
      Hosoe Y, Aoki S, Hori C, Ooi T, Taguchi S, Matsumoto K
    • Organizer
      International Symposium on Biopolymer Synthesis and Degradation
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 新規配列制御型グリコール酸ポリマーの生合成と構造解析2019

    • Author(s)
      梶川彩香、榊原早也果、荒井修造、大井俊彦、堀千明、松本謙一郎
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Research-status Report
  • [Presentation] 植物資源の分解利用へ~メタ環境のオミクス解析・樹木とキノコ(真菌類)の解析2019

    • Author(s)
      堀千明
    • Organizer
      超異分野meetup
    • Related Report
      2019 Research-status Report
  • [Presentation] 生立木が生産する生体防御物質に対する病原性木材腐朽菌の分解メカニズムの解析2019

    • Author(s)
      松本壘、堀千明、宮本敏澄、重冨建吾、佐野雄三、大井俊彦、松本謙一郎
    • Organizer
      第19回糸状菌分子生物学コンファレンス
    • Related Report
      2019 Research-status Report
  • [Presentation] Ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) 反応を経由したグリコール酸ポリマー生合成系とその応用2019

    • Author(s)
      大潟祥梧、永田暁洋、齋藤樹理、横尾俊憲、工藤悠希、堀千明、大井俊彦、田口精一、松本謙一郎
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Research-status Report
  • [Patent(Industrial Property Rights)] 担子菌由来の新規な高機能性リパーゼの利用2021

    • Inventor(s)
      堀 千明
    • Industrial Property Rights Holder
      堀 千明
    • Industrial Property Rights Type
      特許
    • Filing Date
      2021
    • Related Report
      2020 Annual Research Report

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Published: 2019-04-18   Modified: 2023-01-30  

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