The role of copepods as primary producers of polyunsaturated fatty acids

• Project/Area Number: 19K15908
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 40040:Aquatic life science-related
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Kabeya Naoki 東京海洋大学, 学術研究院, 助教 (90758731)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: カイアシ / 多価不飽和脂肪酸 / EPA / DHA / 生合成 / 安定同位体 / 不飽和化酵素 / 鎖長延長酵素

Outline of Research at the Start

カイアシ類（コペポーダ類）にはEPAやDHAといった様々な動物にとって生理的に重要な成分が豊富に含まれていることが知られている。カイアシが魚類の餌生物として非常に重要であることを考慮すると、これらカイアシ類がなぜEPAやDHAを豊富に含むのかを解明することは生態系を栄養学的な観点から理解する上で非常に重要である。そのため本研究では、カイアシ類のEPAやDHAの生合成能を解明することを目的としている。

Outline of Final Research Achievements

The biosynthetic capabilities of polyunsaturated fatty acids (PUFA) in copepods were elucidated in the present project. The copepods were tiny crustacean species mainly inhabiting in the aquatic environment. Some PUFA such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are physiologically essential nutrients for many animals, although most of EPA and DHA are originated from marine environment. Therefore, understanding PUFA production mechanism in the marine ecosystem is important. In the present project, we have confirmed that particular species within copepods possessed high biosynthetic capabilities of PUFA including EPA and DHA, and hence those species could be primary producers of PUFA in the marine ecosystem.

Academic Significance and Societal Importance of the Research Achievements

EPAやDHAは人にとっても重要な栄養素であることが知られている。現状これら栄養素のほとんどは海洋由来であり、海洋生態系においてどのようにこれら栄養素が作り出されるのかを明らかにすることは、これら栄養素を今後持続的に得るために重要な基礎情報となる。これまで、EPAやDHAは基本的に微細藻類などによって合成され、食物連鎖の過程で高次の消費者に蓄積されていくと考えられてきた。本研究では、小型甲殻類であるカイアシの一種から微細藻類と同等のEPAやDHAの生合成経路を有する種を見出した。このことは、海洋におけるEPAやDHAの生産について大幅な見直しが必要であることを示している。

Research Products

• [Int'l Joint Research] Instituto de Acuicultura Torre de la Sal(スペイン)
• [Int'l Joint Research] Instituto de Acuicultura Torre de la Sal(スペイン)
• [Int'l Joint Research] Instituto de Acuicultura Torre de la Sal(スペイン)
• [Journal Article] Expression of long-chain polyunsaturated fatty acids biosynthesis genes during the early life-cycle stages of the tropical gar Atractosteus tropicus (2021)
  • Author(s): De la Cruz-Alvarado Fanny Janet、Alvarez-Gonzalez Carlos A.、Llera-Herrera Raul、Monroig Oscar、Kabeya Naoki、Rodriguez-Morales Sergio、Concha-Frias Bartolo、Guerrero-Zarate Rocio、Jimenez-Martinez Luis D.、Pena-Marin Emyr Saul
  • Journal Title: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Volume: 256 Pages: 110628-110628
  • DOI: 10.1016/j.cbpb.2021.110628
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The evolutionary ecology of fatty‐acid variation: Implications for consumer adaptation and diversification (2021)
  • Author(s): Twining Cornelia W.、Bernhardt Joey R.、Derry Alison M.、Hudson Cameron M.、Ishikawa Asano、Kabeya Naoki、Kainz Martin J.、Kitano Jun、Kowarik Carmen、Ladd Sarah Nemiah、Leal Miguel C.、Scharnweber Kristin、Shipley Jeremy R.、Matthews Blake
  • Journal Title: Ecology Letters Volume: 24 Issue: 8 Pages: 1709-1731
  • DOI: 10.1111/ele.13771
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A complete enzymatic capacity for biosynthesis of docosahexaenoic acid (DHA, 22 : 6n?3) exists in the marine Harpacticoida copepod Tigriopus californicus (2021)
  • Author(s): Kabeya Naoki、Ogino Masanari、Ushio Hideki、Haga Yutaka、Satoh Shuichi、Navarro Juan C.、Monroig Oscar
  • Journal Title: Open Biology Volume: 11 Issue: 4 Pages: 200402-200402
  • DOI: 10.1098/rsob.200402
  • Peer Reviewed / Open Access
• [Journal Article] Biosynthesis of Long-Chain Polyunsaturated Fatty Acids in Marine Gammarids: Molecular Cloning and Functional Characterisation of Three Fatty Acyl Elongases (2021)
  • Author(s): Ribes-Navarro Alberto、Navarro Juan C.、Hontoria Francisco、Kabeya Naoki、Standal Inger B.、Evjemo Jan O.、Monroig Oscar
  • Journal Title: Marine Drugs Volume: 19 Issue: 4 Pages: 226-226
  • DOI: 10.3390/md19040226
  • Peer Reviewed / Open Access
• [Journal Article] Polyunsaturated fatty acid metabolism in three fish species with different trophic level (2021)
  • Author(s): Galindo A.、Garrido D.、Monroig O.、Perez J.A.、Betancor M.B.、Acosta N.G.、Kabeya N.、Marrero M.A.、Bolanos A.、Rodriguez C.
  • Journal Title: Aquaculture Volume: 530 Pages: 735761-735761
  • DOI: 10.1016/j.aquaculture.2020.735761
  • Peer Reviewed
• [Journal Article] Interactive effect of dietary fish oil and pyrimidine nucleotide supplementation on the fatty acid composition of juvenile rainbow trout Oncorhynchus mykiss : Enhancement of ARA and DHA contents in the fillet of fish fed‐supplemented diet (2021)
  • Author(s): Ridwanudin Asep、Kasuya Hitaka、Haga Yutaka、Kabeya Naoki、Satoh Shuichi
  • Journal Title: Aquaculture Research Volume: - Issue: 10 Pages: 4934-4945
  • DOI: 10.1111/are.15327
  • Peer Reviewed
• [Journal Article] Taurine synthesis via the cysteic acid pathway: effect of dietary cysteic acid on growth, body taurine content, and gene expression of taurine-synthesizing enzymes, growth hormone, and insulin-like growth factor 1 in Japanese flounder Paralichthys olivaceus (2021)
  • Author(s): Nakamura Kohei、Gonzales-Plasus Marina Mojena、Ushigusa-Ito Tomoko、Masuda Reiji、Kabeya Naoki、Kondo Hidehiro、Hirono Ikuo、Satoh Shuichi、Haga Yutaka
  • Journal Title: Fisheries Science Volume: 87 Issue: 3 Pages: 353-363
  • DOI: 10.1007/s12562-021-01500-1
  • NAID: 40022567910
  • Peer Reviewed
• [Journal Article] Flatfishes colonised freshwater environments by acquisition of various DHA biosynthetic pathways (2020)
  • Author(s): Matsushita Yoshiyuki、Miyoshi Kaho、Kabeya Naoki、Sanada Shuwa、Yazawa Ryosuke、Haga Yutaka、Satoh Shuichi、Yamamoto Yoji、Str?ssmann Carlos Augusto、Luckenbach John Adam、Yoshizaki Goro
  • Journal Title: Communications Biology Volume: 3 Issue: 1 Pages: 516-516
  • DOI: 10.1038/s42003-020-01242-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity (2020)
  • Author(s): Garrido D.、Monroig O.、Galindo A.、Betancor M.B.、Perez J.A.、Kabeya N.、Marrero M.、Rodriguez C.
  • Journal Title: Aquaculture Volume: 523 Pages: 735147-735147
  • DOI: 10.1016/j.aquaculture.2020.735147
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading (2020)
  • Author(s): Naoki Kabeya, Ibrahim Gur, Angela, Jan Ove Evjemo, Arne M. Malzahn, Francisco Hontoria, Juan C. Navarro, Oscar Monroig
  • Journal Title: Philosophical Transactions of the Royal Society B Volume: 375 Issue: 1804 Pages: 20190654-20190654
  • DOI: 10.1098/rstb.2019.0654
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Diversification of enzymes for biosynthesis of long-chain polyunsaturated fatty acids in aquatic animals: Implications for aquaculture (2019)
  • Author(s): KABEYA NAOKI
  • Journal Title: NIPPON SUISAN GAKKAISHI Volume: 85 Issue: 4 Pages: 386-389
  • DOI: 10.2331/suisan.WA2653
  • NAID: 130007685233
  • ISSN: 0021-5392, 1349-998X
  • Year and Date: 2019-07-15
  • Open Access
• [Journal Article] Functional diversification of teleost Fads2 fatty acyl desaturases occurs independently of the trophic level (2019)
  • Author(s): Garrido Diego、Kabeya Naoki、Betancor Monica B.、Perez Jose A.、Acosta N. Guadalupe、Tocher Douglas R.、Rodriguez Covadonga、Monroig Oscar
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 11199-11199
  • DOI: 10.1038/s41598-019-47709-0
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A key metabolic gene for recurrent freshwater colonization and radiation in fishes (2019)
  • Author(s): Ishikawa Asano, Kabeya Naoki, Ikeya Koki, Kakioka Ryo, Cech Jennifer N., Osada Naoki, Leal Miguel C., Inoue Jun, Kume Manabu, Toyoda Atsushi, Tezuka Ayumi, Nagano Atsushi J., Yamasaki Yo Y., Suzuki Yuto, Kokita Tomoyuki, Takahashi Hiroshi et al.
  • Journal Title: Science Volume: 364 Issue: 6443 Pages: 886-889
  • DOI: 10.1126/science.aau5656
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Functional characterisation of two Δ12-desaturases demonstrates targeted production of linoleic acid as pheromone precursor in Nasonia (2019)
  • Author(s): Florian Semmelmann, Naoki Kabeya, Miriama Malcicka, Astrid Bruckmann, Bastian Broschwitz, Kristina Straub, Rainer Merkl, Oscar Monroig, Reinhard Sterner, Joachim Ruther, Jacintha Ellers
  • Journal Title: The Journal of Experimental Biology Volume: 印刷中
  • DOI: 10.1242/jeb.201038
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Methyl-end desaturases with ?12 and ω3 regioselectivities enable the de novo PUFA biosynthesis in the cephalopod Octopus vulgaris (2019)
  • Author(s): Diego Garrido, Naoki Kabeya, Francisco Hontoria, Juan C Navarro, Diana B Reis, M Virginia Martin, Covadonga Rodriguez, Eduardo Almansa, Oscar Monroig
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids Volume: 1864 Issue: 8 Pages: 1134-1144
  • DOI: 10.1016/j.bbalip.2019.04.012
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Develop non-fish meal and non-fish oil grow-out diet for Asian seabass (Lates calcarifer). (2022)
  • Author(s): Chainark, P., Obtian, J., Yuttayong, D., Kabeya, N., Haga, Y., Satoh, S.
  • Organizer: International Symposium on Fish Nutrition and Feeding 2020
  • Int'l Joint Research
• [Presentation] Purine nucleotide affects fatty acid profile of rainbow trout (Oncorhynchus mykiss) through regulation of srebp-1 in the liver and its downstream factors such as fatty acid desaturase and elongase. (2022)
  • Author(s): Tansutaphanit, S., Haga, Y., Kabeya, N., Kondo, H., Hirono, I., Satoh, S.
  • Organizer: International Symposium on Fish Nutrition and Feeding 2020
  • Int'l Joint Research
• [Presentation] Does enzyme complex treatment improve feed performance of rice protein concentrate-based low fishmeal diet for rainbow trout (Oncorhynchus mykiss) ? (2022)
  • Author(s): Yaman, G., Ueda, S., Takino, T., Kabeya, N., Haga, Y., Satoh, S.
  • Organizer: International Symposium on Fish Nutrition and Feeding 2020
  • Int'l Joint Research
• [Presentation] Cysteic acid pathway: Biosynthesis of taurine in Japanese flounder, Paralichthys olivaceus. (2022)
  • Author(s): Nakamura, K., Gonzales-Plasus, M.M., Masuda, R., Kondo, H., Hirono, I., Kabeya, N., Satoh, S., Haga, Y.
  • Organizer: International Symposium on Fish Nutrition and Feeding 2020
  • Int'l Joint Research
• [Presentation] Kabeya, N., Haga, Y., Satoh, S. Biosynthesis of long-chain polyunsaturated fatty acids in an amphidromous fish, ayu sweetfish Plecoglossus altivelis: Functional characterisation of fatty acid desaturase and elongase. (2022)
  • Author(s): Kabeya, N., Haga, Y., Satoh, S.
  • Organizer: International Symposium on Fish Nutrition and Feeding 2020
  • Int'l Joint Research
• [Presentation] Freshwater colonization of three flatfish families is underpinned by functional plasticity of fatty acid desaturase enabling DHA biosynthesis. (2022)
  • Author(s): Matsushita, Y., Kabeya, N., Chiba, M., Yazawa, R., Luckenbach, J.A., Yoshizaki, G.
  • Organizer: International Symposium on Fish Nutrition and Feeding 2020
  • Int'l Joint Research
• [Presentation] Evaluation of yellow mealworm, (Tenebrio molitor) as a partial replacement of plant-based ingredients in the non-fish meal diets for red sea bream (Pagrus major). (2022)
  • Author(s): Seong, T., Feng, S., Kabeya, N., Haga, Y., Satoh, S.
  • Organizer: International Symposium on Fish Nutrition and Feeding 2020
  • Int'l Joint Research
• [Presentation] Biosynthesis of taurine in fish. (2022)
  • Author(s): Haga, Y., Ushigusa-Itoh, T., Nakamura, K., Watanabe, R., Kabeya, N., Kondo, H., Hirono, I., Satoh, S.
  • Organizer: International Symposium on Fish Nutrition and Feeding 2020
  • Int'l Joint Research
• [Presentation] Can Artemia produce essential fatty acids? Roles of elongases in the biosynthesis of long-chain polyunsaturated fatty acids (2021)
  • Author(s): Marc Ramos-Llorens, Alberto Ribes-Navarro, Juan Carlos Navarro, Francisco Hontoria, Naoki Kabeya, Oscar Monroig
  • Organizer: Aquaculture Europe 2021
  • Int'l Joint Research
• [Presentation] Investigating the biosynthesis of long-chain polyunsaturated fatty acids in marine gammarids (2021)
  • Author(s): Alberto Ribes-Navarro, Juan C. Navarro, Francisco Hontoria, Naoki Kabeya, Inger B. Standal, Jan O. Evjemo, Oscar Monroig
  • Organizer: Aquaculture Europe 2021
  • Int'l Joint Research
• [Presentation] Molecular cloning and functional characterisation of three fatty acyl elongases from the marine gammarid Echinogammarus marinus. (2021)
  • Author(s): Ribes-Navarro, A., Navarro, J.C., Hontoria, F., Kabeya, N., Standal, I.B., Evjemo, J.O., Monroig O.
  • Organizer: Lipids in the Ocean, 2nd Edition
  • Int'l Joint Research
• [Presentation] 多毛類イシイソゴカイ Perinereis wilsoniより単離した多価不飽和脂肪酸生合成酵素の機能解析 (2021)
  • Author(s): 末澤道宏, 壁谷尚樹, 一ノ瀬智士, 小南友里, 芳賀穣, 潮秀樹, 佐藤秀一
  • Organizer: 第44回日本分子生物学会年会
• [Presentation] 甲殻類ヨコエビの一種 Melita sp.より単離した脂肪酸鎖長延長酵素の機能解析 (2021)
  • Author(s): 中野陽, 壁谷尚樹, 一ノ瀬智士, 小南友里, 芳賀穣, 潮秀樹, 佐藤秀一
  • Organizer: 第44回日本分子生物学会年会
• [Presentation] ヨコエビ・ゴカイ類における多価不飽和脂肪酸（PUFA）生合成酵素遺伝子の探索 (2021)
  • Author(s): 一ノ瀬智士, 小南友里, 潮秀樹, 中野陽, 末澤道宏, 壁谷尚樹, 芳賀穣, 佐藤秀一
  • Organizer: 第44回日本分子生物学会年会
• [Presentation] Effects of fishmeal replacement with an enzyme complex-treated rice protein concentrate on growth performance and feed utilization of rainbow trout. (2021)
  • Author(s): Gunes Yaman, Shinya Ueda, Tadashi Takino, Naoki Kabeya, Shuichi Satoh, Yutaka Haga
  • Organizer: 令和3年度日本水産学会秋季大会
• [Presentation] 新規EPA・DHA供給源の探索：①ヨコエビ類の利用性検討 (2021)
  • Author(s): 中野陽, 壁谷尚樹, 一ノ瀬智士, 小南友里, 芳賀穣, 潮秀樹, 佐藤秀一
  • Organizer: 令和3年度日本水産学会秋季大会
• [Presentation] 新規EPA・DHA供給源の探索：②ゴカイ類の利用性検討 (2021)
  • Author(s): 末澤道宏, 壁谷尚樹, 一ノ瀬智士, 小南友里, 芳賀穣, 潮秀樹, 佐藤秀一
  • Organizer: 令和3年度日本水産学会秋季大会
• [Presentation] 海産ハルパクチクス目カイアシ Tigriopus californicus におけるDHA生合成経路の解明 (2021)
  • Author(s): 壁谷尚樹, 荻野将成, 潮秀樹, 芳賀穣, 佐藤秀一, Navarro, J.C., Monroig, O
  • Organizer: 令和3年度日本水産学会春季大会
• [Presentation] Methyl-end desaturases in the common ragworm demonstrate unique activities enabling the production of various omega-3 polyunsaturated fatty acids via multiple pathways (2021)
  • Author(s): Naoki Kabeya, Ibrahim Gur, Angela Oboh, Jan O. Evjemo, Arne M. Malzahn, Francisco Hontoria, Juan C. Navarro, Oscar Monroig
  • Organizer: ISSFAL 2021 VIRTUAL CONGRESS
  • Int'l Joint Research
• [Presentation] 海産肉食性回遊魚であるサンマは完全なDHA合成経路をもつ (2020)
  • Author(s): 松下芳之, 川村亘, 壁谷尚樹, 矢澤良輔, 吉崎悟朗
  • Organizer: 日本水産学会大会
• [Presentation] 植物性飼料原料の栄養価改善に及ぼすエクストルージョン温度条件の影響 (2020)
  • Author(s): SANTIZO-TAAN R., KABEYA N., HAGA Y., SATOH S.
  • Organizer: 日本水産学会大会
• [Presentation] ニジマスOncorhynchus mykiss幼魚の成長と脂肪酸代謝におよぼす低魚粉と魚油を基にした餌料へのプリンおよびヌクレオチド補給の影響 (2020)
  • Author(s): RIDWANUDIN Asep, KABEYA Naoki, HAGA Yutaka, SATOH Shuichi
  • Organizer: 日本水産学会大会
• [Presentation] Metabolismo lipidico de la tenca (Tinca tinca): Estudio de la capacidad de biosintesis de omega-3 LC-PUFA (2019)
  • Author(s): Garrido D, Monroig O, Betancor MB, Perez JA, Kabeya N, Giraldez I, Marrero M, Rodriguez C
  • Organizer: XVII Congreso Nacional de Acuicultura
• [Presentation] Genes for de novo EPA and DHA biosynthesis are present in the harpacticoid copepod Tigriopus californicus (2019)
  • Author(s): Kabeya N, Monroig O, Ushio H
  • Organizer: Marine Biotechnology Conference 2019
  • Int'l Joint Research
• [Remarks] Naoki KABEYA
  • URL: https://sites.google.com/view/naoki-kabeya/