Development of definitive diagnostic tools against equine trypanosomoses

• Project/Area Number: 19K15972
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 42020:Veterinary medical science-related
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: Suganuma Keisuke 帯広畜産大学, 畜産学部, 助教 (60772184)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2020: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: トリパノソーマ / 馬 / 家畜動物 / ウマ / 診断 / トリパノソーマ症 / ゲノム科学

Outline of Research at the Start

ウマトリパノソーマ症は二種類の異なるウマトリパノソーマ原虫の感染により引き起こされる致死的な原虫病である。ウマトリパノソーマ症対策のポイントは2種類の病原原虫種の鑑別と、病原原虫に合わせた清浄化プログラムの実施であるが、種特異性の低い共通抗原を用いているため、現在の診断法では鑑別診断が不可能である。そのため不適切な清浄化プログラムの実施により感染が拡大している。そこで本研究では複数のウマトリパノソーマ株の遺伝情報を比較解析した結果を元に、臨床現場で使用可能な簡便な鑑別診断法（イムノクロマトグラフィー法）を開発することを目的とする。

Outline of Final Research Achievements

Equine trypanosomosis caused by Trypanosoma equiperdum and T. evansi is a fatal protozoan diseases. To establish differential diagnosis methods for equine trypanosomosis, I have performed comparative genomic analysis of T. equiperdum IVM-t1 strain (isolated in Mongolia) and other animal trypanosomes (provided from Institute of Tropical Medicine, Belgium). In addition, epidemiological analysis and risk factor analysis of equine and animal trypanosomosis in developing countries, such as Paraguay, Sudan, and the Philippines, were performed. These results will contribute to establish of effective control strategies against animal trypanosomosis.

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的意義は、経済的価値が高く疾病対応が急務であるが有効な鑑別診断法のないウマトリパノソーマ症に対する鑑別診断法の開発を行い、また実際の検体を用いて開発した鑑別診断法の実効性を検討した点である。
また社会的意義として、動物トリパノソーマ症汚染国である熱帯・亜熱帯地域の発展途上国を対象として、多くの経済被害が出ているウマトリパノソーマ症を含む動物トリパノソーマ症に対する疫学調査を実施し、トリパノソーマ感染リスク因子候補を見出した。それらのデータをもとに科学的知見に基づくトリパノソーマ症対策の実行が期待される。

Research Products

• [Int'l Joint Research] スーダン科学技術大学(スーダン)
• [Int'l Joint Research] セブ科学技術大学(フィリピン)
• [Int'l Joint Research] 獣医学研究所(モンゴル)
• [Int'l Joint Research] 獣医学研究所(モンゴル)
• [Int'l Joint Research] スーダン科学技術大学(スーダン)
• [Int'l Joint Research] セブ科学技術大学(フィリピン)
• [Int'l Joint Research] 獣医学研究所(モンゴル)
• [Int'l Joint Research] ノースウエスト大学(南アフリカ)
• [Int'l Joint Research] スーダン科学技術大学(スーダン)
• [Journal Article] First molecular survey of animal trypanosomes in Paraguayan horses (2022)
  • Author(s): Suganuma Keisuke、Acosta Tomas J.、Valinotti Maria Fatima Rodriguez、Sanchez Antonio Rodr?guez、Mossaad Ehab、Elata Afraa、Inoue Noboru
  • Journal Title: Veterinary Parasitology: Regional Studies and Reports Volume: 27 Pages: 100664-100664
  • DOI: 10.1016/j.vprsr.2021.100664
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] First molecular detection and identification of Trypanosoma evansi in goats from Cebu, Philippines using a PCR-based assay (2020)
  • Author(s): Elata Afraa、Galon Eloiza May、Moumouni Paul Franck Adjou、Ybanez Rochelle Haidee D.、Mossaad Ehab、Salces Caro B.、Bajenting Guindolino P.、Ybanez Adrian P.、Xuan Xuenan、Inoue Noboru、Suganuma Keisuke
  • Journal Title: Veterinary Parasitology: Regional Studies and Reports Volume: 21 Pages: 100414-100414
  • DOI: 10.1016/j.vprsr.2020.100414
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Treatment Efficiency of Combination Therapy With Diminazene Aceturate and Quinapyramine Sulfate in a Horse With Dourine (2020)
  • Author(s): Davkharbayar Batbold、Davaasuren Batdorj、Narantsatsral Sandagdorj、Battur Banzragch、Punsantsogvoo Myagmarsuren、Battsetseg Badgar、Mizushima Daiki、Inoue Noboru、Suganuma Keisuke
  • Journal Title: Journal of Equine Veterinary Science Volume: 87 Pages: 102905-102905
  • DOI: 10.1016/j.jevs.2019.102905
  • NAID: 120007004080
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Serological and molecular detection of selected hemoprotozoan parasites in donkeys in West Omdurman, Khartoum State, Sudan (2020)
  • Author(s): ELATA Afraa、MOSSAAD Ehab、SATTI Rawan、MATAR Nadia、OHARI Yuma、XUAN Xuenan、INOUE Noboru、SUGANUMA Keisuke
  • Journal Title: Journal of Veterinary Medical Science Volume: 82 Issue: 3 Pages: 286-293
  • DOI: 10.1292/jvms.19-0534
  • NAID: 130007805839
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Prevalence of different trypanosomes in livestock in Blue Nile and West Kordofan States, Sudan (2020)
  • Author(s): Mossaad Ehab、Ismail Ahmed Ali、Ibrahim Abdalla Mohamed、Musinguzi Peter、Angara Tamador E.E.、Xuan Xuenan、Inoue Noboru、Suganuma Keisuke
  • Journal Title: Acta Tropica Volume: 203 Pages: 105302-105302
  • DOI: 10.1016/j.actatropica.2019.105302
  • NAID: 120006980457
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Molecular characterization of a new Trypanosoma (Megatrypanum) theileri isolate supports the two main phylogenetic lineages of this species in Japanese cattle (2019)
  • Author(s): Suganuma Keisuke、Kondoh Daisuke、Sivakumar Thillaiampalam、Mizushima Daiki、Elata Afra’a Tajelsir Mohamed、Thekisoe Oriel M. M.、Yokoyama Naoaki、Inoue Noboru
  • Journal Title: Parasitology Research Volume: 118 Issue: 6 Pages: 1927-1935
  • DOI: 10.1007/s00436-019-06313-x
  • NAID: 120006708984
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Equine trypanosomosis: enigmas and diagnostic challenges (2019)
  • Author(s): Buscher Philippe、Gonzatti Mary Isabel、Hebert Laurent、Inoue Noboru、Pascucci Ilaria、Schnaufer Achim、Suganuma Keisuke、Touratier Louis、Van Reet Nick
  • Journal Title: Parasites & Vectors Volume: 12 Issue: 1
  • DOI: 10.1186/s13071-019-3484-x
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Dourine and Trypanosoma equiperdum in Mongolia (2021)
  • Author(s): Keisuke Suganuma and Noboru Inoue
  • Organizer: Veterinary Science - Sustainable cooperation
  • Int'l Joint Research / Invited
• [Presentation] パラグアイで飼養されている馬におけるトリパノソーマ感染に関する疫学調査 (2021)
  • Author(s): 山崎 藍、菅沼啓輔、齊藤朋子、Tomas J. Acosta
  • Organizer: 日本ウマ科学会第34回学術集会
• [Book] 寄生虫病学 第３版 (2020)
  • Author(s): 日本獣医寄生虫学会
  • Total Pages: 276
  • Publisher: 緑書房
  • ISBN: 9784895315692
• [Book] 家畜伝染病ハンドブック (2020)
  • Author(s): 村上 賢二、彦野 弘一
  • Total Pages: 304
  • Publisher: 朝倉書店
  • ISBN: 9784254460384
• [Remarks] 原虫病研究センターHP
  • URL: https://www.obihiro.ac.jp/facility/protozoa/
• [Patent(Industrial Property Rights)] トリパノソーマ症の治療薬及び予防薬 (2020)
  • Inventor(s): 菅沼啓輔
  • Industrial Property Rights Holder: 菅沼啓輔
  • Industrial Property Rights Type: 特許
  • Filing Date: 2020