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Fine-scale phylogenetic diversity and underlying mechanisms of coexisting oxymonad protists in termite guts

Research Project

Project/Area Number 19K16208
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 45030:Biodiversity and systematics-related
Research InstitutionTokyo Institute of Technology

Principal Investigator

Igai Katsura  東京工業大学, 生命理工学院, 研究員 (60806438)

Project Period (FY) 2019-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords種多様性 / 種分化 / 多種共存 / 共生 / シロアリ / 原生生物 / 多様性 / エコタイプ / 腸内微生物叢 / シングルセル
Outline of Research at the Start

シロアリの高効率な木質分解能力の大部分は、1.5億年以上前から腸内に共生する木質分解性原生生物の働きによる。シロアリ腸内には複数種の原生生物が生息し、例えばヤマトシロアリ腸内には16種もが共生する。なぜ同じ資源を餌とする生物が複数種共存しうるのだろうか。本課題は、ヤマトシロアリ祖先系統の腸内原生生物群集はオオシロアリから水平伝播したという知見に着目し、疑問解明を目指す。オオシロアリを含むシロアリ4種の腸内原生生物群集について高解像度な分子系統解析を行い、その多様性と分岐の系譜を明らかにする。さらに、ごく近縁な原生生物数種の網羅的転写産物解析を行い、多種共存を成立させうる機能分化を検証する。

Outline of Final Research Achievements

To elucidate the true diversity and underlying mechanism of the coexistence of oxymonad symbiont community in guts of the termite Reticulitermes speratus, this study preformed fine-scale phylogenetic analysis, in silico simulation of speciation, and examination of interspecies niche differentiation of the protist community. The results strongly suggested the possibilities that phylogenetically closely related but distinct 33 oxymonad species coexist in a gut of Reticulitermes speratus and that the predicted species correspond with ecotypes with different niches.

Academic Significance and Societal Importance of the Research Achievements

本研究は難培養性のシロアリ腸内原生生物の種多様性と多種共存機構について新たな研究方法と知見を提示した。種とはなにかまた自然界の多様な多種共存機構の理解は、生物学および生態学の根幹的テーマである。本研究の成果は、シロアリ腸内原生生物ばかりでなく、近年発展著しい原生生物(全般)の種多様性研究に貴重な知見を付与するものである。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (10 results)

All 2022 2021 2020 2019

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Journal Article] Fine‐scale genetic diversity and putative ecotypes of oxymonad protists coinhabiting the hindgut of Reticulitermes speratus2021

    • Author(s)
      Igai Katsura、Kitade Osamu、Fu Jieyang、Omata Kazumi、Yonezawa Takahiro、Ohkuma Moriya、Hongoh Yuichi
    • Journal Title

      Molecular Ecology

      Volume: 31 Issue: 4 Pages: 1317-1331

    • DOI

      10.1111/mec.16309

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] シロアリ腸内原生生物の種多様性を見つめなおす2019

    • Author(s)
      猪飼 桂
    • Journal Title

      しろあり

      Volume: 172 Pages: 62-66

    • NAID

      40021982414

    • Related Report
      2019 Research-status Report
  • [Presentation] Dynamics of symbiotic protist community in termite guts associated with caste differentiation and vertical transmission process2022

    • Author(s)
      Tatsuya Inagaki, Katsura Igai, Kazuki Takahashi, Yuichi Hongoh
    • Organizer
      19th International Union for the Study of Social Insects Congress (IUSSI)
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Dynamics of gut microbiota in termites associated with host's adult eclosion and dispersal2022

    • Author(s)
      Tatsuya Inagaki, Katsura Igai, Kazuki Takahashi, Yuichi Hongoh
    • Organizer
      10th Congress of the International Society of Symbiosis, 3rd International Conference on Holobionts
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] シロアリ腸内共生原生生物群集におけるエコタイプ・シミュレーション解析2021

    • Author(s)
      猪飼 桂, 北出 理, 傅 潔洋, 尾又一実, 米澤隆弘, 大熊盛也, 本郷裕一
    • Organizer
      日本微生物生態学会第34回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] シロアリの王・女王特異的な腸内微生物群集動態2020

    • Author(s)
      稲垣辰哉, 猪飼桂, 本郷裕一, 松浦健二
    • Organizer
      日本共生学会第4回大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Royal-specific gut microbiota in termites2020

    • Author(s)
      Tatsuya Inagaki, Katsura Igai, Yuichi Hongoh, Kenji Matsuura
    • Organizer
      第68回日本生態学会大会
    • Related Report
      2020 Research-status Report
  • [Presentation] Fine-scale phylogenetic structure and putative ecotypes in the oxymonad community in Reticulitermes speratus.2019

    • Author(s)
      Igai K., Kitade O., Fu J., Ohkuma M., Hongoh Y.
    • Organizer
      OIST Mini Symposium “Ecology and Evolution of Termite Gut Microbes”
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヤマトシロアリ腸内オキシモナス群集におけるエコタイプ仮説の検証2019

    • Author(s)
      猪飼 桂、北出 理、傅 潔洋、大熊 盛也、本郷 裕一
    • Organizer
      原生生物学会
    • Related Report
      2019 Research-status Report
  • [Presentation] シロアリ腸内原生生物はどこから来たのか? 何者か? どこに行くのか?2019

    • Author(s)
      猪飼 桂
    • Organizer
      原生生物・寄生虫・進化セミナー
    • Related Report
      2019 Research-status Report
    • Invited

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Published: 2019-04-18   Modified: 2024-01-30  

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