Research of resting state functional connectivity by neural, astrocyte and hemodynamic activity measurements

• Project/Area Number: 19K16252
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 46010:Neuroscience-general-related
• Research Institution: Okayama University (2021); The University of Tokyo (2019-2020)
• Principal Investigator: Matsui Teppei 岡山大学, 自然科学学域, 准教授 (10725948)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2020: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2019: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: カルシウムイメージング / 安静時脳活動 / 機能結合 / 機能的MRI / 脳活動イメージング / 大脳皮質 / アストロサイト

Outline of Research at the Start

機能的磁気共鳴画像法(fMRI)による安静時脳活動の計測(RS-fMRI)は、ヒト大脳ネットワークの機能構造の解明や、精神・神経疾患による大脳ネットワークの変性を検出する技術として研究開発が進んでいる。本研究では、申請者らが最近開発したイメージング技術を駆使して、神経活動・アストロサイト活動・脳血流の関係を調べ、RS-fMRIの神経基盤を明らかにする。

Outline of Final Research Achievements

We constructed a system for imaging the arousal state for the measurement of neural activity and cerebral blood flow. In addition, in the state of spontaneous brain activity, it was observed that astrocyte activity was induced following spontaneous neural activity, and that astrocyte activity suppressed the subsequent spontaneous neural activity. Regarding resting state brain activity, the effect of drug administration was observed as a change in functional connection in both neural activity and cerebral blood flow, but difference was seen in the change in neural activity and cerebral blood flow. It was found that the spatial specificity did not occur clearly in the cerebral blood flow. These results are considered to be important results for understanding the neural basis of resting brain activity.

Academic Significance and Societal Importance of the Research Achievements

機能的磁気共鳴画像法(fMRI)による安静時脳活動の計測(RS-fMRI)は、ヒト大脳皮質ネットワークの機能構造の解明や、精神・神経疾患による大脳ネットワークの変性を検出する技術として臨床応用も視野に入れた研究開発が進んでいる。しかしながらfMRIは脳血流を計測する技術であるため、RS-fMRIで計測される機能的結合がどの程度まで実際の神経活動を反映したものであるかは未だ良く分かっていない。本研究で得られた成果は、fMRIで計測できる安静時における神経細胞とアストロサイトの相互作用、および脳血流との関係性を明らかにした点で臨床応用に不可欠な基礎的知見であると考えられる。

Research Products

• [Journal Article] Counterfactual Explanation of Brain Activity Classifiers Using Image-To-Image Transfer by Generative Adversarial Network (2022)
  • Author(s): Matsui Teppei、Taki Masato、Pham Trung Quang、Chikazoe Junichi、Jimura Koji
  • Journal Title: Frontiers in Neuroinformatics Volume: 15 Pages: 802938-802938
  • DOI: 10.3389/fninf.2021.802938
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Executive control by fronto-parietal activity explains counterintuitive decision behavior in complex value-based decision-making (2022)
  • Author(s): Matsui Teppei、Hattori Yoshiki、Tsumura Kaho、Aoki Ryuta、Takeda Masaki、Nakahara Kiyoshi、Jimura Koji
  • Journal Title: NeuroImage Volume: 249 Pages: 118892-118892
  • DOI: 10.1016/j.neuroimage.2022.118892
  • Peer Reviewed / Open Access
• [Journal Article] On co-activation pattern analysis and non-stationarity of resting brain activity (2022)
  • Author(s): Matsui Teppei、Pham Trung Quang、Jimura Koji、Chikazoe Junichi
  • Journal Title: NeuroImage Volume: 249 Pages: 118904-118904
  • DOI: 10.1016/j.neuroimage.2022.118904
  • NAID: 120007188354
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Multiscale calcium imaging of spontaneous activity in the primate visual cortical network. (2020)
  • Author(s): T. Matsui, T. Hashimoto, T. Murakami, M. Uemura, K.Kikuta, T. Kato, K. Ohki
  • Organizer: 生理学研究所研究会「意思決定研究の新展開,（生理学研究所）
  • Invited
• [Presentation] Multiscale calcium imaging of spontaneous activity in the primate visual cortical network (2020)
  • Author(s): T. Matsui, T. Hashimoto, T. Murakami, M. Uemura, K.Kikuta, T. Kato, K. Ohki
  • Organizer: 日本神経科学大会国際公募シンポジウム
  • Int'l Joint Research / Invited
• [Presentation] Coupling and decoupling between neural activity and hemodynamics revealed by multispectral imaging (2020)
  • Author(s): R. Li, T. Matsui, K. Ohki
  • Organizer: 日本神経科学大会
  • Int'l Joint Research
• [Presentation] Development and Application of Multi-Scale Ca2+ Imaging in the Primate Visual Cortical Network (2020)
  • Author(s): Matsui, T., Hashimoto, T., Uemura, M., Murakami, T., Kikuta, K., Kato, T., Ohki, K.
  • Organizer: The 8th RIEC International Symposium on Brain Functions and Brain Computer
  • Int'l Joint Research / Invited
• [Presentation] Spatiotemporal structure of spontaneous activity in the marmoset visual cortex (2020)
  • Author(s): Matsui, T., Hashimoto, T., Uemura, M., Murakami, T., Kikuta, K., Kato, T., Ohki, K.
  • Organizer: 日本生理学会大会
• [Presentation] Spatiotemporal structure of spontaneous activity in the primate visual cortex (2019)
  • Author(s): Matsui, T., Hashimoto, T., Uemura, M., Murakami, T., Kikuta, K., Kato, T., Ohki, K.
  • Organizer: 神経ダイナミクスミニワークショップ
  • Int'l Joint Research / Invited
• [Presentation] 新しい神経活動イメージング法の開発による脳のマルチモーダル・マルチスケール動作原理の解明 (2019)
  • Author(s): Matsui T
  • Organizer: 高知工科大学招待講演
  • Invited
• [Presentation] Structured spontaneous activity in the marmoset visual cortex studied with in vivo calcium imaging (2019)
  • Author(s): Matsui, T., Hashimoto, T., Uemura, M., Murakami, T., Kikuta, K., Kato, T., Ohki, K.
  • Organizer: 日本神経科学大会
• [Book] 脳の半分を占める グリア細胞 (2019)
  • Author(s): 松井鉄平、大木研一
  • Total Pages: 230
  • Publisher: 羊土社
  • ISBN: 9784758103824