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Experimental infection model of Dengue virus and Zika virus in northern Japanese local mosquitoes

Research Project

Project/Area Number 19K16675
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 49060:Virology-related
Research InstitutionRakuno Gakuen University

Principal Investigator

Uchida Leo  酪農学園大学, 獣医学群, 講師 (50756723)

Project Period (FY) 2019-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2020: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2019: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsジカウイルス / 蚊 / 感受性 / 媒介能 / 次世代シークエンス / 変異 / 適合性 / デングウイルス / 北方系ヤブカ / ウイルス変異 / Quasispecies
Outline of Research at the Start

近年、大規模な気候変動、急速な交通網の発達を背景にデング熱、ジカ熱といった蚊媒介感染症が拡大を見せている。これらを引き起こすウイルスは主にネッタイシマカやヒトスジシマカといった南方に分布する蚊と人との間で感染が維持されるが、日本を含む北方に分布するヤブカでのウイルス感受性については情報が乏しい。そこで本研究では、北海道に分布するヤブカを用い、上記のウイルスの感受性、媒介能を明らかにすると共に、蚊の体内で起こるウイルスの変異を同定する。

Outline of Final Research Achievements

This study aimed to evaluate the susceptibility and vector competence of Aedes japonicus from northern Japan to Zika virus (ZIKV), which causes febrile illness and microcephaly to human. After oral infection of ZIKV, the amplified virus was detected from several parts of the body of Ae. japonicus, and the viral RNA was detected from the saliva of the mosquitoes. The infection rate was lower than two major ZIKV vector, Ae. albopictus and Ae. aegypti, but the virus titer in the body was almost same level or higher than the two mosquito species. Besides, next generation sequencing targeting on ZIKV genome which derived from the virus-infected mosquitoes revealed that two amino acid mutations accumulate in the viral envelope protein. The two amino acid mutations imply that regulate N-linked glycosylation of envelope protein.

Academic Significance and Societal Importance of the Research Achievements

ジカウイルスは2015年、南米を中心とした世界的な流行が見られ、近年、日本国内への侵入が危惧される蚊媒介性ウイルスである。本研究では日本に広く分布するヤマトヤブカにおいて、程度は低いながらもウイルスが媒介され得ることを明らかにした。また、本研究で見つかったウイルスの変異は、ウイルスが蚊で増殖する際の適合性に関わる因子と考えられ、今後、蚊媒介性ウイルス関連の研究へ展開が見込まれる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (14 results)

All 2022 2021 2020 2019

All Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (8 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Seroepidemiological surveys of tick-borne encephalitis virus and novel tick-borne viruses in wild boar in Nagasaki, Japan2022

    • Author(s)
      Luvai Elizabeth Ajema Chebichi、Uchida Leo、Tun Mya Myat Ngwe、Inoue Shingo、Weiyin Hu、Shimoda Hiroshi、Morita Kouichi、Hayasaka Daisuke
    • Journal Title

      Ticks and Tick-borne Diseases

      Volume: 13 Issue: 1 Pages: 101860-101860

    • DOI

      10.1016/j.ttbdis.2021.101860

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Zika Virus Potential Vectors among Aedes Mosquitoes from Hokkaido, Northern Japan: Implications for Potential Emergence of Zika Disease2021

    • Author(s)
      Uchida Leo、Shibuya Miki、Morales-Vargas Ronald Enrique、Hagiwara Katsuro、Muramatsu Yasukazu
    • Journal Title

      Pathogens

      Volume: 10 Issue: 8 Pages: 938-938

    • DOI

      10.3390/pathogens10080938

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Isolation and Characterization of Mosquito-Associated Spiroplasma cantharicola from Aedes japonicus Collected in Hokkaido, Japan2021

    • Author(s)
      Shimooka Makoto、Sakurai Yoshimi、Muramatsu Yasukazu、Uchida Leo
    • Journal Title

      Insects

      Volume: 12 Issue: 12 Pages: 1056-1056

    • DOI

      10.3390/insects12121056

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 北海道の野生動物を用いたマダニ媒介性ウイルス調査と新規マダニウイルスの検索2021

    • Author(s)
      内田 玲麻
    • Journal Title

      地域ケアリング

      Volume: 23 Pages: 43-47

    • NAID

      40022476792

    • Related Report
      2020 Research-status Report
  • [Journal Article] Effect of chemical tick control practices on tick infestation and Theileria parva infection in an intensive dairy production region of Uganda2020

    • Author(s)
      Miyama Takeshi、Byaruhanga Joseph、Okamura Ikuo、Uchida Leo、Muramatsu Yasukazu、Mwebembezi William、Vudriko Patrick、Makita Kohei
    • Journal Title

      Ticks and Tick-borne Diseases

      Volume: 11 Issue: 4 Pages: 101438-101438

    • DOI

      10.1016/j.ttbdis.2020.101438

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] FTA-Sodium hydroxide-based polymerase chain reaction (PCR): An efficient and cheaper option for <i>Theileria parva</i> detection in dairy cattle in Mbarara, Uganda2020

    • Author(s)
      UCHIDA Leo, BYARUHANGA Joseph, OKAMURA Ikuo, MIYAMA Takeshi, MURAMATSU Yasukazu, VUDRIKO Patrick, MAKITA Kohei
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 82 Issue: 2 Pages: 188-192

    • DOI

      10.1292/jvms.19-0521

    • NAID

      130007799359

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 北海道のヤブカにおけるアルボウイルスの検索2021

    • Author(s)
      内田玲麻、櫻井愛美、村松康和
    • Organizer
      第164回日本獣医学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ヤマトヤブカ体内におけるジカウイルスのQuasispecies評価2021

    • Author(s)
      三木要、岩本夏奈、江下優樹、大場靖子、澤洋文、村松康和、内田玲麻
    • Organizer
      第164回日本獣医学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 北海道産ヤマトヤブカにおけるジカウイルス感受性の評価2021

    • Author(s)
      川江詩乃、岩本夏奈、江下優樹、大場靖子、澤洋文、村松康和、内田玲麻
    • Organizer
      第164回日本獣医学会学術集会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Zika Virus Potential Vectors among Aedes Mosquitoes from Northern Japan2021

    • Author(s)
      Leo Uchida, Ronald Enrique Morales-Vargas, Katsuro Hagiwara, Yasukazu Muramatsu, Yuki Eshita, Yasuko Orba, Hirofumi Sawa
    • Organizer
      Joint International Tropical Medicine Meeting (JITMM) Virtual 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 野鳥の節足動物媒介性病原体の検出2021

    • Author(s)
      益桃子、佐々木瑞希、村松康和、内田玲麻
    • Organizer
      日本寄生虫学会・日本衛生動物学会 第67回北日本支部合同大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 北海道のマダニにおける吸血源とマダニ媒介性ウイルスの調査2019

    • Author(s)
      小柳優奈、内田玲麻、村松康和
    • Organizer
      第162回日本獣医学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Identification of a novel phlebovirus gene in Ixodes persulcatus and Haemaphysalis flava collected in Hokkaido, Japan2019

    • Author(s)
      内田玲麻、小柳優奈、村松康和
    • Organizer
      第67回日本ウイルス学会学術集会
    • Related Report
      2019 Research-status Report
  • [Presentation] Development of of portable LAMP for JEV2019

    • Author(s)
      Shiela L. Dumuk-Buenaventura、内田玲麻、村松康和
    • Organizer
      第9回家畜感染症学会
    • Related Report
      2019 Research-status Report

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Published: 2019-04-18   Modified: 2023-01-30  

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