Analysis between RNA modification and pathogenesis in myelodysplastic syndromes
| Project/Area Number |
19K16988
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 52010:General internal medicine-related
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| Research Institution | International University of Health and Welfare |
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Principal Investigator |
Shibuta Tatsuki 国際医療福祉大学, 福岡保健医療学部, 講師 (20799192)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 骨髄異形成症候群 / RNA修飾 / NGS解析 |
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| Outline of Research at the Start |
高齢化に伴い生じる造血器腫瘍に対する早期診断および治療は非常に困難であり，現在の日本において大きな課題の1つである。骨髄異形成症候群は年間5,000例以上が発症する造血器腫瘍で，その多くは60歳以上の高齢者で増加傾向にある。
本研究においては骨髄異形成症候群の細胞内で生じるRNA修飾異常に焦点を当て次世代シーケンサーを用いた解析を実施し，さらに修飾酵素の変異・タンパク発現解析を行う。
高齢者における造血器腫瘍の早期発見，身体的負担の少ない継続治療可能な診断法および薬剤の開発を目指す。
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| Outline of Final Research Achievements |
Cytosine methylation in mRNA and RNA methyltransferases in myelodysplastic syndrome (MDS) were analyzed. Compared to CD34-positive cells, MDS cells showed increased expression of transcription factors and granulocyte lineage-specific genes, and increased methylated cytosine in the untranslated region. In addition, NSUN2, which is a RNA methyltransferase, was highly expressed in MDS cells, and its expression level was involved in cell proliferatin.
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| Academic Significance and Societal Importance of the Research Achievements |
MDSには芽球比率による病期が存在するが、NSUN2 mRNAがMDSでの病期を推測するための病態進展マーカーとして有用となり、侵襲性の高い検査の回避や検査者の習熟が必要な形態診断を補助できる可能性が高い。
またNSUN2阻害剤によりMDSの進展を抑制できる可能性があり、今後さらに検討する必要がある。
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Report
(4 results)
Research Products
(16 results)
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[Journal Article] Pre-Analytical Modification of Serum miRNAs: Diagnostic Reliability of Serum miRNAs in Hemolytic Diseases2021
Author(s)
Yukichi Takada, Tatsuki Shibuta, Mayu Hatano, Kenichi Sato, Mari Koga, Ayaka Ishibashi, Tetsuhiro Harada, Takashi Hisatomi, Hanae Shimura, Noriyasu Fukushima, Kamonlak Leecharoenkiat,Supat Chamnanchanunt, Saovaros Svasti, Suthat Fucharoen and Tsukuru Umemura
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Journal Title
J. Clin. Med.
Volume: 10
Issue: 21
Pages: 692008-692008
DOI
Related Report
Peer Reviewed / Open Access
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