• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a novel kidney regeneration using the SWPU system

Research Project

Project/Area Number 19K17722
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 53040:Nephrology-related
Research InstitutionJikei University School of Medicine

Principal Investigator

Yokote Shinya  東京慈恵会医科大学, 医学部, 助教 (30459656)

Project Period (FY) 2019-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2020: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords腎臓再生 / 後腎移植 / 後腎
Outline of Research at the Start

申請者は、再生腎臓の尿排泄路システムであるStepwise Peristaltic Ureter system : SWPU systemを開発した。今回の研究では、SWPU systemを用いて再生した新規腎臓をさらに大型化し尿量を増加させる方法を開発し、臨床応用に向けての研究基盤を確立することが目標である。
新規腎臓を大型化するために①VEGF等の液性因子や間葉系幹細胞の新規腎臓の発育への影響を精査し、②利尿剤であるTolvaptanが新規腎臓の尿量増加に寄与するかを検討する。また、③無腎ラットの長期予後を改善しうるかについても検討することを目的とする。

Outline of Final Research Achievements

1) Analysis of the effect of HIF-PH inhibitor administration on the development of regenerated kidneys.
Metanephroi with a bladder (cloaca) was transplanted into LEWIS rats. Rats were divided into two groups. The HIF-PH group received Roxadustat and the control group received a sham drug. SWPU was performed 4 weeks after transplantation. 8 weeks after transplantationnew kidney weights and renal histology were evaluated. Although new kidney weight was increased in the HIF-PH group compared to the control group, there was no obvious significant difference in new kidney weight between the two groups. 2) Effect of Tolvaptan on regenerating kidneys. LEWIS cloaca is transplanted into rats. Rats were divided into 2 groups. The tolvaptan group received oral administration of tolvaptan, while the control group received received a sham drug orally. At 4 weeks after transplantation, SWPU was performed. tolvaptan group showed a trend toward increased urinary cloaca volume.

Academic Significance and Societal Importance of the Research Achievements

SWPUを用いた再生腎臓の大型化、尿量の増加を可能にする薬剤についての検討を行った。ある種の薬剤には再生腎臓の大型化や尿量の増加が期待できるという本研究の結果は、SWPUを用いた腎臓再生が臨床応用するために重要な所見であったと思われる。今後、研究を継続し、これらの知見を用いて実際にSWPUを用いた腎臓再生により腎不全動物の予後を改善することができれば、さらにSWPUを用いた腎臓再生の臨床応用に近づくと思われる。

Report

(6 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (2 results)

All 2019

All Journal Article (2 results)

  • [Journal Article] Clinical and Pathological Characteristics of Elderly Japanese Patients with IgA Vasculitis with Nephritis: A Case Series2019

    • Author(s)
      Hiroyuki Ueda 1 , Yoichi Miyazaki 1 , Nobuo Tsuboi 1 , Keita Hirano 1 , Shinya Yokote 1 , Emi Kobayashi 2 , Makoto Ogura 1 , Tetsuya Kawamura 1 , Munekazu Ryuzaki 2 , Takashi Yokoo
    • Journal Title

      Internal Medicine

      Volume: 58 Issue: 1 Pages: 31-38

    • DOI

      10.2169/internalmedicine.1379-18

    • NAID

      130007542252

    • ISSN
      0918-2918, 1349-7235
    • Year and Date
      2019-01-01
    • Related Report
      2019 Research-status Report
  • [Journal Article] Negative Impact of Proteinuria on Circulating Myeloid Dendritic Cells2019

    • Author(s)
      Masato Ikeda 1 , Risa Terashima 2 , Taku Yamada 3 , Masahiro Suyama 3 , Shinya Yokote 3 , Masatsugu Nakao 2 , Izumi Yamamoto 2 , Keita Hirano 2 , Hideo Okonogi 2 , Hiroyasu Yamamoto 2 , Takashi Yokoo
    • Journal Title

      Clinical and Experimental Nephrology

      Volume: 23 Issue: 7 Pages: 928-938

    • DOI

      10.1007/s10157-019-01724-7

    • Related Report
      2019 Research-status Report

URL: 

Published: 2019-04-18   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi