Analysis of immunocyte dynamics for identifying refractory predictive biomarkers in pediatric rheumatic diseases

• Project/Area Number: 19K17908
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 54020:Connective tissue disease and allergy-related
• Research Institution: Yamaguchi University
• Principal Investigator: Wakiguchi Hiroyuki 山口大学, 大学院医学系研究科, 助教 (00716590)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 小児リウマチ / フローサイトメトリー / 末梢血 / 関節液 / バイオマーカー

Outline of Research at the Start

若年性特発性関節炎、全身性エリテマトーデス、若年性皮膚筋炎、シェーグレン症候群および川崎病に代表される小児リウマチ性疾患は、原因不明の難治性免疫疾患である。その病態解明には免疫担当細胞の解析が望まれるが、報告は未だ不十分である。病態を解明し、難治性を予測するバイオマーカーを見出せれば、適切な治療が患者に提供される。そこで、本研究では、小児リウマチ性疾患における難治性予測バイオマーカーの確立を目的に、治療前後および寛解増悪時の患者末梢血および関節液免疫担当細胞数を網羅的にフローサイトメトリーで測定し、その動態を解析する。

Outline of Final Research Achievements

I have established a sample collection system from the pediatric rheumatic diseases and a system for measuring the number of immunocytes. However, the number of samples is not enough to analyze the results. Although the planned research period has ended, I will continue to collect samples steadily and measure and analyze the number of immunocytes.

Academic Significance and Societal Importance of the Research Achievements

小児リウマチ性疾患における免疫担当細胞の網羅的解析により、難治性予測バイオマーカーが発見できる可能性があること。

Research Products

• [Journal Article] Successful treatment of children with juvenile systemic sclerosis using mycophenolate mofetil after methylprednisolone pulse therapy: A 3‐year follow‐up (2022)
  • Author(s): Furusawa Akinori、Wakiguchi Hiroyuki、Okazaki Fumiko、Korenaga Yuno、Azuma Yoshihiro、Yasudo Hiroki、Hasegawa Shunji
  • Journal Title: International Journal of Rheumatic Diseases Volume: 25 Issue: 1 Pages: 95-96
  • DOI: 10.1111/1756-185x.14260
  • Peer Reviewed
• [Journal Article] A novel mutation in early‐onset sarcoidosis/Blau syndrome: an association with Propionibacterium acnes (2021)
  • Author(s): Okazaki Fumiko、Wakiguchi Hiroyuki、Korenaga Yuno、Nakamura Tamaki、Yasudo Hiroki、Uchi Shohei、Yanai Ryoji、Asano Nobuyuki、Hoshii Yoshinobu、Tanabe Tsuyoshi、Izawa Kazushi、Honda Yoshitaka、Nishikomori Ryuta、Uchida Keisuke、Eishi Yoshinobu、Ohga Shouichi、Hasegawa Shunji
  • Journal Title: Pediatric Rheumatology Volume: 19 Issue: 1 Pages: 18-18
  • DOI: 10.1186/s12969-021-00505-5
  • Peer Reviewed / Open Access
• [Journal Article] Juvenile Neuropsychiatric Systemic Lupus Erythematosus With Magnetic Resonance Imaging Findings Similar to Acute Necrotizing Encephalopathy (2020)
  • Author(s): Korenaga Yuno、Wakiguchi Hiroyuki、Matsushige Takeshi、Inoue Hirofumi、Mizutani Makoto、Furuta Takashi、Kawamura Mai、Nakamura Tamaki、Okazaki Fumiko、Yasudo Hiroki、Maki Takashi、Hasegawa Shunji
  • Journal Title: JCR: Journal of Clinical Rheumatology Volume: - Issue: 8S Pages: 1-1
  • DOI: 10.1097/rhu.0000000000001698
  • Peer Reviewed
• [Journal Article] Multispecialty approach for improving outcomes in juvenile dermatomyositis (2019)
  • Author(s): Wakiguchi Hiroyuki
  • Journal Title: Journal of Multidisciplinary Healthcare Volume: Volume 12 Pages: 387-394
  • DOI: 10.2147/jmdh.s171095
  • Peer Reviewed / Open Access
• [Presentation] 小児リウマチ性疾患における免疫担当細胞の網羅的解析に向けて (2020)
  • Author(s): 脇口 宏之
  • Organizer: 第24回リウマチフォーラム
  • Invited
• [Presentation] 小児リウマチ性疾患における免疫担当細胞の網羅的解析に向けて (2019)
  • Author(s): 脇口 宏之
  • Organizer: 第6回JCRベーシックリサーチカンファレンス
  • Invited