Clinical study on oral-health management in sustaining the condition of inflammatory bowel disease patients in remission

• Project/Area Number: 19K19339
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57080:Social dentistry-related
• Research Institution: Tsurumi University
• Principal Investigator: Otsuka Ryoko 鶴見大学, 歯学部, 非常勤講師 (00803701)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2020: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2019: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 炎症性腸疾患 / 口腔保健管理 / 次世代シークエンス解析 / 唾液カルプロテクチン / ELISA / カルプロテクチン

Outline of Research at the Start

炎症性腸疾患の患者数は増加の一途をたどり、厚生労働省の定める特定疾患（難病）の中で1位となった。急増した理由も根本原因に関しても依然として不明ではあるが、腸管免疫系に大きく影響する腸内細菌叢の「均衡破綻と多様性の消失；dysbiosis」の関与が有力視されている。近年では、腸内細菌叢のdysbiosisに口腔病原細菌の関与を示唆した研究が相次いで報告されており、「口腔細菌叢と腸内細菌叢との連関」に関心が高まっている。以上の背景から、炎症性腸疾患患者への歯科的介入（口腔保健管理）により、口腔・腸内細菌叢に及ぼす影響をメタゲノム解析により検証する。

Outline of Final Research Achievements

A chronic immune-mediated inflammatory disease of the gastrointestinal tract known as inflammatory bowel disease (IBD) is being widely investigated with growing attention for possible involvement of oral pathogens. This study focused on the effects of a progressive oral-health care on the conditions of IBD patients in remission comparing with healthy volunteers. In addition, salivary calprotectin; a calcium-binding antimicrobial protein complex was targeted aiming to assess the inflammatory activity in IBD patients to monitor disease activity as a biomarker instead of fecal calprotectin.
It was indicated that a progressive oral-health management including self-oral care using a custom-tray and hypochlorous acid electrolyzed water (twice a day) contributes to the improvement of the oral environment of IBD patients in remission. Salivary calprotectin might be a useful marker for monitoring IBD disease state.

Academic Significance and Societal Importance of the Research Achievements

炎症性腸疾患（IBD）は、再燃と寛解を繰り返す病態であるため、寛解期が長く続けば患者の生活の質は上がり、負担も大きく軽減される。そのためには継続的な病態把握が可能なマーカーの探索は必要不可欠である。本研究で採用した唾液カルプロテクチンはすでに保険収載されている便中カルプロテクチンと同様の傾向を示した。得られた知見は、今後のIBDバイオマーカ―を対象とした臨床研究に寄与することが考えられる。

Research Products

• [Journal Article] Evaluation of the cariogenic potential of a probiotic candidate strain Lactobacillus gasseri YIT 12321 (2022)
  • Author(s): Okada Ayako、Imai Susumu、Kikuchi Tomohiro、Matin Khairul、Otsuka Ryoko、Terai Tomohiko、Okumura Takekazu、Yamamoto Takatsugu、Hanada Nobuhiro
  • Journal Title: Archives of Oral Biology Volume: 136 Pages: 105364-105364
  • DOI: 10.1016/j.archoralbio.2022.105364
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Effect of advanced periodontal self-care in patients with early-stage periodontal diseases on endothelial function: An open-label, randomized controlled trial (2021)
  • Author(s): Okada Ayako、Murata Takatoshi、Matin Khairul、Ariyoshi Meu、Otsuka Ryoko、Yamashita Mamiko、Suzuki Masayuki、Wakiyama Rumi、Tateno Ken、Suzuki Megumi、Aoyagi Hitomi、Uematsu Hiromi、Imamura Akiko、Kosaka Miki、Mizukaki Tomoko、Sato Tsutomu、Kawahara Hiroshi、Hanada Nobuhiro
  • Journal Title: PLOS ONE Volume: 16 Issue: 9 Pages: 0257247-0257247
  • DOI: 10.1371/journal.pone.0257247
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Structure and Validity of Questionnaire for Oral Frail Screening (2021)
  • Author(s): Nomura Yoshiaki、Ishii Yoshimasa、Chiba Yota、Suzuki Shunsuke、Suzuki Akira、Suzuki Senichi、Morita Kenji、Tanabe Joji、Yamakawa Koji、Ishiwata Yasuo、Ishikawa Meu、Sogabe Kaoru、Kakuta Erika、Okada Ayako、Otsuka Ryoko、Hanada Nobuhiro
  • Journal Title: Healthcare Volume: 9 Issue: 1 Pages: 45-45
  • DOI: 10.3390/healthcare9010045
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Does Last Year’s Cost Predict the Present Cost? An Application of Machine Leaning for the Japanese Area-Basis Public Health Insurance Database (2021)
  • Author(s): Nomura Yoshiaki、Ishii Yoshimasa、Chiba Yota、Suzuki Shunsuke、Suzuki Akira、Suzuki Senichi、Morita Kenji、Tanabe Joji、Yamakawa Koji、Ishiwata Yasuo、Ishikawa Meu、Sogabe Kaoru、Kakuta Erika、Okada Ayako、Otsuka Ryoko、Hanada Nobuhiro
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 18 Issue: 2 Pages: 565-565
  • DOI: 10.3390/ijerph18020565
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nutritional Status and Oral Frailty: A Community Based Study (2020)
  • Author(s): Nomura Yoshiaki、Ishii Yoshimasa、Suzuki Shunsuke、Morita Kenji、Suzuki Akira、Suzuki Senichi、Tanabe Joji、Ishiwata Yasuo、Yamakawa Koji、Chiba Yota、Ishikawa Meu、Sogabe Kaoru、Kakuta Erika、Okada Ayako、Otsuka Ryoko、Hanada Nobuhiro
  • Journal Title: Nutrients Volume: 12 Issue: 9 Pages: 2886-2886
  • DOI: 10.3390/nu12092886
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Workforce and Contents of Home Dental Care in Japanese Insurance System (2020)
  • Author(s): Nomura Yoshiaki、Okada Ayako、Kakuta Erika、Otsuka Ryoko、Saito Hideaki、Maekawa Hiroshi、Daikoku Hideki、Hanada Nobuhiro、Sato Tamotsu
  • Journal Title: International Journal of Dentistry Volume: 2020 Pages: 1-6
  • DOI: 10.1155/2020/7316796
  • Peer Reviewed / Open Access
• [Journal Article] Oral Microbiome of Children Living in an Isolated Area in Myanmar (2020)
  • Author(s): Nomura Y, Otsuka R, Hasegawa R, Hanada N.
  • Journal Title: Int J Environ Res Public Health. Volume: 5;17(11) Issue: 11 Pages: 4033-4033
  • DOI: 10.3390/ijerph17114033
  • Peer Reviewed / Open Access
• [Journal Article] Tooth-Level Analysis of Dental Caries in Primary Dentition in Myanmar Children (2020)
  • Author(s): Nomura Yoshiaki、Otsuka Ryoko、Wint Wit Yee、Okada Ayako、Hasegawa Ryo、Hanada Nobuhiro
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 17 Issue: 20 Pages: 7613-7613
  • DOI: 10.3390/ijerph17207613
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mortality- and Health-Related Factors in a Community-Dwelling of Oldest-Older Adults at the Age of 90: A 10-Year Follow-Up Study (2020)
  • Author(s): Nomura Yoshiaki、Shimada Mieko、Kakuta Erika、Okada Ayako、Otsuka Ryoko、Tomizawa Yasuko、Taguchi Chieko、Arikawa Kazumune、Daikoku Hideki、Sato Tamotsu、Hanada Nobuhiro
  • Journal Title: International Journal of Environmental Research and Public Health Volume: 17 Issue: 24 Pages: 9584-9584
  • DOI: 10.3390/ijerph17249584
  • Peer Reviewed / Open Access
• [Journal Article] Effects of self-assessed chewing ability, tooth loss and serum albumin on mortality in 80-year-old individuals: a 20-year follow-up study (2020)
  • Author(s): Nomura Yoshiaki、Kakuta Erika、Okada Ayako、Otsuka Ryoko、Shimada Mieko、Tomizawa Yasuko、Taguchi Chieko、Arikawa Kazumune、Daikoku Hideki、Sato Tamotsu、Hanada Nobuhiro
  • Journal Title: BMC Oral Health Volume: 20 Issue: 1 Pages: 122-122
  • DOI: 10.1186/s12903-020-01113-7
  • Peer Reviewed / Open Access
• [Journal Article] Impact of the Serum Level of Albumin and Self-Assessed Chewing Ability on Mortality, QOL, and ADLs for Community-Dwelling Older Adults at the Age of 85: A 15 Year Follow up Study (2020)
  • Author(s): Nomura Yoshiaki、Kakuta Erika、Okada Ayako、Otsuka Ryoko、Shimada Mieko、Tomizawa Yasuko、Taguchi Chieko、Arikawa Kazumune、Daikoku Hideki、Sato Tamotsu、Hanada Nobuhiro
  • Journal Title: Nutrients Volume: 12 Issue: 11 Pages: 3315-3315
  • DOI: 10.3390/nu12113315
  • Peer Reviewed / Open Access
• [Journal Article] Properties of manual toothbrush that influence on plaque removal of interproximal surface in?vitro (2020)
  • Author(s): Otsuka R, Nomura Y, Okada A, Uematsu H, Nakano M, Hikiji K, Hanada N, Momoi Y
  • Journal Title: J Dent Sci Volume: 15 Issue: 1 Pages: 14-21
  • DOI: 10.1016/j.jds.2019.07.005
  • Peer Reviewed / Open Access
• [Journal Article] Real time measurement of stress by electroencephalogram during contentious mouth opening. (2019)
  • Author(s): Otsuka R, Nomura Y, Okada A, Uraguchi M, Tadokoro H, Nagai T, Fujii Y, Miura M, Kawachi R, Yamamoto M, Wakana T and Hanada N.
  • Journal Title: Asian Pac J Dent Volume: 19 Pages: 45-50
  • Peer Reviewed / Open Access
• [Journal Article] Consistency of supplied food and dentition status of the elderly in residential care homes. (2019)
  • Author(s): Nomura Y , Okada A , Kakuta E , Otsuka R , Sogabe K , Yamane K , Yamamoto T , Shigeta Y , Shigemoto S , Ogawa T , Hanada N
  • Journal Title: BMC Oral Health Volume: 19 Issue: 1 Pages: 74-74
  • DOI: 10.1186/s12903-019-0770-0
  • Peer Reviewed / Open Access
• [Journal Article] PRECEDE-PROCEED model based questionnaire and saliva tests for oral health checkup in adult (2019)
  • Author(s): Nomura Y , Matsuyama T , Fukai K , Okada A , Ida M , Yamauchi N , Hanamura H , Yabuki Y , Watanabe K , Sugawara M , Imanishi Y , Koizumi N , Murano Y , Nishiyama A , Fukukawa Y , Otsuka R , Hanada N
  • Journal Title: Journal of Oral Science Volume: 61 Issue: 4 Pages: 544-548
  • DOI: 10.2334/josnusd.18-0288
  • NAID: 130007751518
  • ISSN: 1343-4934, 1880-4926
  • Peer Reviewed / Open Access
• [Journal Article] Medical-dental and hospital-clinic collaboration in Iwate Prefecture in Japan. (2019)
  • Author(s): Okada A, Nomura Y, Kakuta E, Otsuka R, Maekawa H, Daikoku H, Hanada N, Sato T.
  • Journal Title: Asian Pac J Dent Volume: 19 Pages: 77-84
  • Peer Reviewed / Open Access
• [Presentation] ミュータンスレンサ球菌とLactobacillus gasseriの相互作用について (2022)
  • Author(s): 大塚良子、菊地朋宏、岡田彩子、今井 奬、花田信弘
  • Organizer: 第11回口腔保健用機能性食品研究会・総会
• [Presentation] 全身的な健康を歯科から考える“予防医学”としての概念を歯科へ「3DS除菌外来」の試み. (2019)
  • Author(s): 山田秀則，宮之原真由，大塚良子，石川芽生，曽我部 薫，岡田彩子，武内博朗, 村田貴俊，野村義明，花田信弘.
  • Organizer: 第68回日本口腔衛生学会・総会
• [Presentation] 血管内皮機能に対する歯周組織健康状態改善の有効性評価：ランダム化並行群間比較試験. (2019)
  • Author(s): 岡田彩子，村田貴俊，有吉芽生，大塚良子，山下万美子，鈴木 恵，青柳ひとみ, 植松裕美，今村安芸子，佐藤 勉，マティン カイルール，花田信弘.
  • Organizer: 第68回日本口腔衛生学会・総会
• [Presentation] 唾液検査を用いた歯周病のスクリーニング検査による地域健康診断システム構築. (2019)
  • Author(s): 大祢貴俊，上谷公之，矢吹義秀，福澤洋一，古藤真実，吉野浩和，三穂乙暁，西辻直之，谷村秀樹，長井博昭，角田衣理加，大塚良子，岡本公彰，岡田彩子，山下万美子，村田貴俊，野村義明，花田信弘.
  • Organizer: 第68回日本口腔衛生学会・総会