Identification and characterization of teleost plasma cells

• Project/Area Number: 19K21158
• Project/Area Number (Other): 18H06019 (2018)
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Multi-year Fund (2019); Single-year Grants (2018)
• Review Section: 0603:Forestry and forest products science, applied aquatic science, and related fields
• Research Institution: Fukui Prefectural University
• Principal Investigator: Takizawa Fumio 福井県立大学, 海洋生物資源学部, 准教授 (60822913)
• Project Period (FY): 2018-08-24 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 抗体 / 形質細胞 / B細胞 / ニジマス / 魚類 / ワクチン

Outline of Research at the Start

ワクチンによる感染予防には、異物である抗原を排除する抗体の産生を誘導し、免疫記憶を伴う獲得免疫を成立させることが重要である。形質細胞は、抗体を産生する主要な細胞であり、抗原存在下で白血球であるB細胞から分化する。つまり、この分化機構を促進させることが、感染症に対して効果的なワクチン開発において重要だと考えられる。そこで本研究では、魚類の形質細胞を同定・分取し、その特性を明らかにするとともに、B細胞から形質細胞への分化機構を解明する。

Outline of Final Research Achievements

Vaccine is a fundamental component of disease prevention in aquaculture and is thought to work by inducing antibody capable of binding specifically to a pathogen. Plasma cells are main antibody-producing cells, which arise from B cells upon antigen stimulation. To isolate and characterize teleost plasma cells, we developed novel and reliable method to identify plasma cells in rainbow trout by using anti-trout IgM and anti-trout IgD antibodies in combination with cell size and endoplasmic reticulum. The plasma-like cells identified in this research exhibit dense nucleus and voluminous cytoplasm, characteristic morphology in human plasma cells. Moreover, they expressed high Blimp1 transcripts while low Pax5 transcripts, a master regulator of plasma cells and mature B cells, respectively. Thus, these findings revealed that teleost plasma cells possess characters similar to those in human and provide useful methods to identify plasma cells in teleost fish.

Academic Significance and Societal Importance of the Research Achievements

魚類のB細胞から形質細胞への分化機構はほとんど分かっておらず、ワクチン投与魚においても主要な抗体産生である形質細胞が誘導されているか分かっていない。そこで本研究は、ニジマスを用いて魚類の形質細胞の同定法を発見し、ヒトの形質細胞と同様な特徴を有していることを明らかにしたものである。本研究成果により、魚類におけるB細胞から形質細胞への分化機構の解析やワクチン投与魚における形質細胞の動態を調べることが可能になり、より効果的な水産用ワクチンの開発に貢献できる。

Research Products

• [Journal Article] Specialization of mucosal immunoglobulins in pathogen control and microbiota homeostasis occurred early in vertebrate evolution (2020)
  • Author(s): Xu Zhen、Takizawa Fumio、Casadei Elisa、Shibasaki Yasuhiro、Ding Yang、Sauters Thomas J. C.、Yu Yongyao、Salinas Irene、Sunyer J. Oriol
  • Journal Title: Science Immunology Volume: 5 Issue: 44
  • DOI: 10.1126/sciimmunol.aay3254
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Teleost cytotoxic T cells (2019)
  • Author(s): Yamaguchi Takuya、Takizawa Fumio、Furihata Mitsuru、Soto-Lampe Veronica、Dijkstra Johannes M.、Fischer Uwe
  • Journal Title: Fish & Shellfish Immunology Volume: 95 Pages: 422-439
  • DOI: 10.1016/j.fsi.2019.10.041
  • Peer Reviewed / Int'l Joint Research
• [Presentation] アニサキスの簡易迅速な種判別法の開発 (2020)
  • Author(s): 末武弘章・瀧澤文雄・宮台俊明
  • Organizer: 令和2年度日本水産学会春季大会
• [Presentation] ニジマスIgTによる鰓の常在細菌叢の制御 (2020)
  • Author(s): 瀧澤文雄・Zhen Xu・Elisa Casadei・Irene Salinas・Yang Ding・柴崎康宏・J. Oriol Sunyer
  • Organizer: 令和2年度日本水産学会春季大会
• [Presentation] ニジマスIgM+ 形質細胞の同定 (2020)
  • Author(s): 原田理沙・瀧澤文雄・猿田裕典・宮台俊明・末武弘章
  • Organizer: 令和2年度日本水産学会春季大会
• [Presentation] メラノマクロファージは末梢血由来か？ (2020)
  • Author(s): 林忠弘・関澤大輝・中山宙・小高智之・瀧澤文雄・宮台俊明・末武弘章
  • Organizer: 令和2年度日本水産学会春季大会
• [Presentation] IDENTIFICATION OF PRIMORDIAL ORGANIZED LYMPHOID STRUCTURE IN THE SPLEEN OF TELEOST FISH (2020)
  • Author(s): J. Oriol Sunyer, Yasuhiro Shibasali, Fumio Takizawa, Ding Yang, Pierre Boudinot, Aleksei Krasnov.
  • Organizer: IMMUNOLOGY2020
  • Int'l Joint Research
• [Presentation] ニジマス海面養殖時に発生するビブリオ病 (2019)
  • Author(s): 末武弘章・小髙智之・瀧澤文雄・井戸篤史・西木一生・吉田照豊・宮台俊明
  • Organizer: 平成31年度日本魚病学会春季大会
• [Presentation] ニジマスのCD4+ マクロファージの同定および特性解析 (2019)
  • Author(s): 瀧澤文雄・Zhen Xu・柴崎康宏・J. Oriol Sunyer
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] 魚類メラノマクロファージの両能性 (2019)
  • Author(s): 林忠弘・関澤大輝・小高智之・瀧澤文雄・宮台俊明・末武弘章
  • Organizer: 平成31年度日本水産学会春季大会
• [Presentation] 真骨魚類の転写因子Spi-Cは哺乳類とは異なる機能を持つ (2019)
  • Author(s): 林忠弘、関澤大輝、小高智之、瀧澤文雄、宮台俊明、末武弘章
  • Organizer: 第 31 回日本比較免疫学会学術集会
• [Presentation] 魚類特有の転写因子Spic-L (2019)
  • Author(s): 林 忠弘・関澤大輝・中山 宙・小高智之・瀧澤文雄・宮台俊明・末武弘章
  • Organizer: 令和元年度日本水産学会秋季大会
• [Presentation] DISCOVERY OF CYTOTOXIC KILLER IGT+ B CELLS IN FISH (2019)
  • Author(s): Yasuhiro Shibasaki, Fumio Takizawa, Michael Gonzalez, Pierre Boudinot, J. Oriol Sunyer.
  • Organizer: 8th North American Comparative Immunology Workshop
  • Int'l Joint Research
• [Presentation] IDENTIFICATION OF PRIMORDIAL ORGANIZED LYMPHOID STRUCTURE IN THE SPLEEN OF TELEOST FISH (2019)
  • Author(s): Yasuhiro Shibasaki, Fumio Takizawa, Yang Ding, Pierre Boudinot, Aleksei Krasnov, J. Oriol Sunyer
  • Organizer: 3rd INTERNATIONAL CONFERENCE ON FISH & SHELLFISH IMMUNOLOGY
  • Int'l Joint Research
• [Presentation] PERFORIN-EXPRESSING IGT+ B CELL WITH CYTOTOXIC ACTIVITY, A NOVEL PLAYER IN THE INNATE IMMUNE RESPONSE OF TELEOST FISH (2019)
  • Author(s): Yasuhiro Shibasaki, Fumio Takizawa, Yang Ding, Pierre Boudinot, J. Oriol Sunyer
  • Organizer: 3rd INTERNATIONAL CONFERENCE ON FISH & SHELLFISH IMMUNOLOGY
  • Int'l Joint Research
• [Presentation] Discovery of perforin-expressing killer B cells in vertebrates (2019)
  • Author(s): J. Oriol Sunyer, Yasuhiro Shibasaki, Fumio Takizawa, Michael Gonzalez, Pierre Boudinot.
  • Organizer: IMMUNOLOGY2019
  • Int'l Joint Research
• [Presentation] Interactions between microbiota and the teleost immune system in health and disease, (2018)
  • Author(s): Irene Salinas, Elisa Casadei, Fumio Takizawa, Yasuhiro Shibasaki, Oriol J Sunyer.
  • Organizer: Immunology 2018, Austin, TX, USA, May 4-8, 2018
  • Int'l Joint Research
• [Presentation] AN ESSENTIAL ROLE OF IGT IN PATHOGEN CONTROL AND COMMENSAL HOMEOSTASIS IN GUT AND GILL MUCOSAL SURFACES. (2018)
  • Author(s): Fumio Takizawa, Zhen Xu, Yasuhiro SHibasaki, Elisa Casadei, Thomas Sauters, Scott LaPatra, Irene Salinas, J. Oriol Sunyer.
  • Organizer: 14th Congress of the ISDCI, Santa Fe, NM, USA, June 17-21, 2018.
  • Int'l Joint Research
• [Presentation] AN EVOLUTIONARILY CONSERVED INNATE IMMUNE ROLE IDENTIFIED IN VERTEBRATE PLASMA CELLS. (2018)
  • Author(s): Yang Ding, Yasuhiro Shibasaki, Jacob Paiano, Fumio Takizawa, Joel Wilmore, Daniella Gomez, David Allman, J. Oriol Sunyer.
  • Organizer: 14th Congress of the ISDCI, Santa Fe, NM, USA, June 17-21, 2018.
  • Int'l Joint Research
• [Presentation] PERFORIN-EXPRESSING IGT+ B CELL WITH CYTOTOXIC ACTIVITY, A NOVEL PLAYER IN THE INNATE IMMUNE RESPONSE OF TELEOST FISH. (2018)
  • Author(s): Yasuhiro Shibasaki, Fumio Takizawa, Michael Gonzalez, Pierre Boudinot, J. Oriol Sunyer.
  • Organizer: 14th Congress of the ISDCI, Santa Fe, NM, USA, June 17-21, 2018.
  • Int'l Joint Research
• [Presentation] IDENTIFICATION OF PRIMORDIAL SEMI-ORGANIZED LYMPHOID STRUCTURES IN TELEOST FISH. (2018)
  • Author(s): Yasuhiro Shibasaki, Tomas Korytor, Fumio Takizawa, Yang Ding, J. Oriol Sunyer.
  • Organizer: 14th Congress of the ISDCI, Santa Fe, NM, USA, June 17-21, 2018.
  • Int'l Joint Research
• [Presentation] THE SWIMBLADDER PLAYS A PREVIOUSLY UNRECOGNIZED NEW FUNCTION IN MUCOSAL IMMUNITY. (2018)
  • Author(s): Yongyao Yu, Weiguang Kong, Xiaoting Zhang, Fen Dong, Zhenyu Huang, Shuai Dong, Yaxing Yin, Fumio Takizawa, J. Oriol Sunyer, Zhen Xu.
  • Organizer: 14th Congress of the ISDCI, Santa Fe, NM, USA, June 17-21, 2018.
  • Int'l Joint Research
• [Presentation] COLLABORATIVE IMMUNE REAGENT NETWORK FOR AQUACULTURED SPECIES (CIRNAS) - AN UPDATE. (2018)
  • Author(s): John D. Hansen, Fumio Takizawa, Irene Salinas, J. Oriol Sunyer.
  • Organizer: 14th Congress of the ISDCI, Santa Fe, NM, USA, June 17-21, 2018.
  • Int'l Joint Research
• [Presentation] ニジマスIgTによる粘膜面の病原体感染および常在細菌叢の制御 (2018)
  • Author(s): 瀧澤文雄・Zhen Xu・柴崎康宏・Yang Ding・Elisa Casadei・Irene Salinas・J. Oriol Sunyer
  • Organizer: 第30回日本比較免疫学会、神奈川、2018年8月20日～22日
• [Presentation] CD4-1 及び CD4-2 の発現様式による魚類ヘルパーT 細胞亜集団の同定 (2018)
  • Author(s): 瀧澤文雄・柴崎康宏・Zhen Xu・Susana Magadan・Pierre Boudinot・J. Oriol Sunyer
  • Organizer: 第30回日本比較免疫学会、神奈川、2018年8月20日～22日
• [Presentation] ニジマスIL-10の抗炎症作用及びYersinia ruckeri感染症における役割 (2018)
  • Author(s): 瀧澤文雄・Zhen Xu・Yang Ding・柴崎康宏・J. Oriol Sunyer
  • Organizer: 平成30年度日本魚病学会秋季大会、広島、2018年9月13日～14日
• [Presentation] ニジマスの鰓におけるIgTを中心とした液性免疫応答 (2018)
  • Author(s): 瀧澤文雄・Zhen Xu・柴崎康宏・Yang Ding・J. Oriol Sunyer
  • Organizer: 平成30年度日本水産学会秋季大会、広島、2018年9月15日～18日
• [Presentation] 魚類特有の抗原捕捉の場メラノマクロファージセンターは増やせるか？ (2018)
  • Author(s): 林忠弘・関澤大輝・小高智之・瀧澤文雄・末武弘章・宮台俊明
  • Organizer: 平成30年度日本水産学会中部支部大会、新潟、2018年12月15日