| Project/Area Number |
19K21335
|
|---|
| Project/Area Number (Other) |
18H06236 (2018)
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Multi-year Fund (2019)
Single-year Grants (2018) |
|---|
| Review Section |
0905:Surgery of the organs maintaining homeostasis and related fields
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
TAKEI YUSUKE 東北大学, 大学病院, 助教 (80822890)
|
|---|
| Project Period (FY) |
2018-08-24 – 2020-03-31
|
| Project Status |
Completed (Fiscal Year 2019)
|
| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | 人工呼吸関連肺傷害 / 内皮微小粒子 / 血管内皮傷害 / セボフルラン |
|---|
| Outline of Research at the Start |
本課題では、内皮微小粒子（EMPs：endothelial microparticles）が、人工呼吸関連肺傷害（VILI: ventilator induced lung injury) の内皮傷害を反映するか検証する。高容量換気（20 ml/kg）によりVILI 動物モデルを作製し、血中EMPs 表面の内皮抗原発現パターンからVILI の血管内皮傷害を解明する。また、VILI の血管内皮傷害に対するセボフルランの肺保護作用をEMPs の内皮抗原発現パターンから検証する。
|
| Outline of Final Research Achievements |
We reported that lung-derived circulating Endothelial Microparticles (EMPs) increased in acute lung injury, reflecting pulmonary endothelial injury. Furthermore, it were strongly correlated with the severity of vascular hyper-permeability in acute lung injury. In addition, we showed that the number of circulating EMPs increased in patients with sepsis, and that PECAM+EMPs and VE-cadherin+EMPs could be a novel indicator of vascular hyper-permeabilitiy. We established more stable VILI rat model by administering muscle relaxants to eliminate the effect of spontaneous breathing with precise ventilator that enables high volume ventilation of 20 ml / kg accurately for small animals.
|
| Academic Significance and Societal Importance of the Research Achievements |
本課題では、内皮微小粒子(endothelial microparticles: EMPs)が敗血症や急性肺傷害の内皮傷害を反映し、新たな血管内皮マーカーとなり得ることを示した。特に、PECAM+EMPsやVe-cadherin+EMPsは血管透過性亢進の指標になり得ることが示唆されており、人工呼吸関連肺傷害(VILI)の内皮傷害の病態の解明、セボフルランを含めたVILIに対する新規治療の効果判定に役立てられることが期待される。
|
