Identification of tetrodotoxin in marine sediments and screening for its biosynthetic genes in marine soil bacteria
| Project/Area Number |
19K22266
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| Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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| Allocation Type | Multi-year Fund |
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| Review Section |
Medium-sized Section 38:Agricultural chemistry and related fields
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2019-06-28 – 2022-03-31
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| Project Status |
Completed (Fiscal Year 2021)
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| Budget Amount *help |
¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | テトロドトキシン / 麻痺性貝毒 / 生合成 / ホタテガイ |
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| Outline of Research at the Start |
テトロドトキシン(TTX)はフグ毒として知られるが、海洋では他にカニ、タコなど、また陸上ではイモリ、カエルに存在する。起源生物は細菌としているが、どこで膨大なTTXが生産されるのか、なぜ、広範な生物が毒化するのかわかっていない。本研究は、日本でTTXが発生している地域の二枚貝や海底土壌を分析し、TTXが検出されるのか明らかにする。さらに、TTX陽性の土壌中微生物のDNAを抽出し、TTX生合成遺伝子を得ることに挑戦する。
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| Outline of Final Research Achievements |
Tetrodotoxin (TTX) is detected in some marine animals, pufferfish, crabs, octopuses, etc., and bacterial production of TTX has been reported. However, the questions such as, where TTX is produced, and why TTX is widely distributed among marine organisms, have not been well answered yet. Biosynthetic genes of TTX have not been identified neither. In this study, we analyzed TTX in marine sediments, and in the scallops cultured in a bay of East Japan, which were temporally collected for three years. TTX was clearly detected in the marine sediment one time. TTX in the scallops was identified by LCMS for the first time in Japanese bivalves. However, the TTX concentrations in the edible tissues were confirmed to be lower than the restricted concentration proposed by European Food Safety Authority (EFSA). The concentrations of TTX in scallops repeatedly increased from August to October in three years.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、未解明のTTX生合成を解決するために、海底土壌サンプルのTTX分析や、海底付近の微小生物のDNA網羅的解析を行い、TTX生合成遺伝子を探索した。さらに、海水中に吊るして養殖したホタテガイを分析し、微量のTTXを国内で初めてLCMSで同定した。また、TTXは8月-10月にかけてピークを示すことを初めて示した。これらは、海洋生物のTTX毒化メカニズム解明において、学術的意義がある。また、ホタテガイ可食部のTTX濃度は食品衛生的に問題のないレベルであることを明らかにし、社会的意義もある。
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Report
(4 results)
Research Products
(101 results)
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[Journal Article] Synthesis of C12-keto saxitoxin derivatives with unusual inhibitory activity against voltage‐gated sodium channels.2020
Author(s)
Adachi, K., Yamada, T., Ishizuka, H., Oki, M., Tsunogae, S., Shimada, N., Chiba, O., Orihara, T., Hidaka, M., Hirokawa, T., Odagi, M., Konoki, K., Yotsu-Yamashita, M., Nagasawa, K.
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Journal Title
Chem. Eur. J.
Volume: 26
Issue: 9
Pages: 2025-2033
DOI
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Peer Reviewed
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[Journal Article] Metabolomic study of saxitoxin analogues and biosynthetic intermediates in dinoflagellates using 15N-labelled sodium nitrate as a nitrogen source2019
Author(s)
1.Cho, Y., Tsuchiya, S., Omura, T., Koike, K., Oikawa, H., Konoki, K., Oshima, Y., Yotsu-Yamashita, M.
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Journal Title
Sci. Rep.
Volume: 9
Issue: 1
Pages: 3460-3460
DOI
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Peer Reviewed / Open Access
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[Presentation] 渦鞭毛藻における麻痺性貝毒の生合成2022
Author(s)
○長 由扶子, 日出間 志寿, 大村 卓朗, 土屋 成輝, 小池 一彦, 小池 香苗, 及川 寛, 此木 敬一, 大島 泰克, 山下 まり
Organizer
令和4年度日本水産学会春季大会ミニシンポジウム フグ毒と麻痺性貝毒の産生と動態に 関する研究の現状と展望
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[Presentation] Marine and Freshwater Toxins, Detection, Structure, and Pharmacology, Possible mode of action of polycavernosides, and Nav blocking activities of highly purified tetrodotoxin analogues2021
Author(s)
Mari Yotsu-Yamashita*, Yuka Doi, Kotaro Iwasaki, Satori Sasaki, Yuki Kawashima, Noriko Shimada, Osamu Chiba, Kyoka Sato, Yuta Kudo, Yuko Cho, Keiichi Konoki, Makoto Sasaki
Organizer
PACIFICHEM 2021, Symposium #32, Marine and Freshwater Toxins, Detection
Related Report
Int'l Joint Research / Invited
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[Presentation] マイトトキシンの標的分子探索2021
Author(s)
此木敬一, 角替俊輔, 八代田陽子, 松本健, Katherine Chan, Amy Hin, Yan Tong, Kamaldeep, Kaur Aulakh, Andrea Habsid, 山下まり, Jason Moffat, Charles Boone, 吉田稔, 村田道雄
Organizer
化学コミュニケーションのフロンティア第8回公開シンポジウム
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[Presentation] 合成サキシトキシン誘導体に対する電位依存性ナトリウムチャネルの感受性評価2021
Author(s)
千葉修, 山田智士, 角替俊輔, 島田紀子, 長 由扶子, 高柳優夏, 星 美波, 安達栞菜, 石塚 颯, 長澤和夫, 山下まり, 此木敬一
Organizer
日本農芸化学会2021年度大会
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[Presentation] Identification of biosynthetic intermediates of amnesic shellfish toxin domoic acid and anthelmintic compound kainic acid2019
Author(s)
〇Maeno, Y., Kotaki, Y., Terada, R., Cho, Y., Konoki, K. and Yotsu-Yamashita, M.
Organizer
Gordon Research Conference, Mycotoxins and Phycotoxins
Related Report
Int'l Joint Research
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[Presentation] Metabolomic study of saxitoxin biosynthesis in dinoflagellates using 15N-labelled sodium nitrate as a nitrogen source2019
Author(s)
〇Cho, Y., Tsuchiya, S., Omura, T., Koike, K., Oikawa, H., Konoki, K., Oshima, Y. and Yotsu-Yamashita, M.
Organizer
Gordon Research Conference, Mycotoxins and Phycotoxins
Related Report
Int'l Joint Research
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[Presentation] Screening of novel secondary metabolites from microorganisms associated with the marine sponge Halichondria okadai2019
Author(s)
〇Goto, M., Kikuchi, S., Okada, K., Cho, Y., Yotsu-Yamashita, M. and Konoki, K.
Organizer
Tohoku University’s Chemistry Summer School
Related Report
Int'l Joint Research
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[Presentation] Isolation and structural elucidation of tetrodotoxin related compounds from pufferfish2019
Author(s)
〇Yaegashi, Y., Ueyama, N., Kudo, Y., Cho, Y., Konoki, K. and Yotsu-Yamashita, M.
Organizer
Tohoku University’s Chemistry Summer School
Related Report
Int'l Joint Research
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[Presentation] Crisprスクリーニングによる maitotoxin の標的分子探索（２）2019
Author(s)
角替俊輔,Katherine Chan,Amy Hin Yan Tong, Kamaldeep Kaur Aulakh, Andrea Habsid,八代田陽子,Jason Moffat,Charles Boone,山下まり, 村田道雄,〇此木敬一
Organizer
公益社団法人日本農芸化学会東北支部・第154回大会
Related Report
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[Presentation] Crisprスクリーニングによる maitotoxin の標的分子探索（１）2019
Author(s)
〇角替俊輔,Katherine Chan,Amy Hin Yan Tong,Kamaldeep Kaur Aulakh, Andrea Habsid,八代田陽子,Jason Moffat,Charles Boone,山下まり,村田道雄,此木敬一
Organizer
公益社団法人日本農芸化学会東北支部・第154回大会
Related Report
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