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Comprehensive imaging of intracellular dynamics and homemade compounds to elucidate the mechanism of asymmetric division in plants.

Research Project

Project/Area Number 19K22421
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
Research InstitutionTohoku University (2020-2021)
Nagoya University (2019)

Principal Investigator

Ueda Minako  東北大学, 生命科学研究科, 教授 (20598726)

Co-Investigator(Kenkyū-buntansha) 金岡 雅浩  名古屋大学, 理学研究科, 講師 (10467277)
Project Period (FY) 2019-06-28 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2020: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords植物発生学 / ライブイメージング / 化合物スクリーニング / 植物受精卵 / 植物
Outline of Research at the Start

多細胞生物は複雑な構造をもつが、それら全ては単一細胞である受精卵から発生する。しかし植物では、受精卵の内部でどのような変化が起こるのかや、それを制御する因子など、いまだ全く分かっていない。その理由としては、これまで生きた受精卵を観察できる手法が存在しなかったことと、重要な機能を担う遺伝子の欠損株が得られにくいことが挙げられる。そこで本研究では、まず、研究代表者が開発した受精卵のライブイメージング技術を用いて、受精卵の内部動態を精緻に観察する。加えて、このライブイメージング法と化合物スクリーニングを組み合わせることで、従来法では見出し得なかった鍵因子を網羅的に同定する。

Outline of Final Research Achievements

Polarization and unequal division of zygote are the initial points of ontogeny. In plants, however, it has been largely unknown what factors regulate what events inside the zygote. In this study, we performed comprehensive live imaging in Arabidopsis thaliana and found that the huge vacuoles are essential for the regulation of asymmetric divisions in the zygote (Matsumoto et. al., 2021). Chemical screening also led to the discovery of several novel inhibitors that act on various regulations in plant cell division (in preparation).

Academic Significance and Societal Importance of the Research Achievements

研究代表者らは、植物の受精卵が極性化する動態をリアルタイムで観察することに成功した。さらに、細胞内にある液胞のダイナミックな動きや制御が、受精卵の不等分裂だけでなく、その後の形作りにも必須であることも突き止めた。ほとんどの植物において、受精卵は大きな液胞を持つことから、本研究が明らかにした仕組みは、植物に共通した普遍的な機構であると期待される。また、化合物スクリーニングで得られた阻害剤は、多くの植物種に働くことも判明したので、農業技術への応用など、さまざまな分野への展開が期待できる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (24 results)

All 2021 2020 2019 Other

All Int'l Joint Research (6 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 4 results) Presentation (7 results) (of which Int'l Joint Research: 6 results,  Invited: 7 results) Remarks (4 results)

  • [Int'l Joint Research] Wageningen University(オランダ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of Warwick(英国)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Institute of Plant Sciences Paris Saclay(フランス)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Max Planck Institute(ドイツ)

    • Related Report
      2020 Research-status Report
  • [Int'l Joint Research] Institute of Plant Sciences Paris Saclay(フランス)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] University of Warwick(英国)

    • Related Report
      2019 Research-status Report
  • [Journal Article] Dynamic Rearrangement and Directional Migration of Tubular Vacuoles are Required for the Asymmetric Division of the <i>Arabidopsis</i> Zygote2021

    • Author(s)
      Matsumoto Hikari、Kimata Yusuke、Higaki Takumi、Higashiyama Tetsuya、Ueda Minako
    • Journal Title

      Plant and Cell Physiology

      Volume: 62 Issue: 8 Pages: 1280-1289

    • DOI

      10.1093/pcp/pcab075

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] What is quantitative plant biology?2021

    • Author(s)
      Autran D., Bassel GW., Chae E., Ezer D., Ferjani A., Fleck C., Hamant O., Hartmann FP., Jiao Y., Johnston IG., Kwiatkowska D., Lim BL., Mahnen AP., Morris RJ., Mulder BM., Nakayama N., Sozzani R., Strader LC., Tusscher K., Ueda M., Wolf S.
    • Journal Title

      Quantitative Plant Biology

      Volume: 2 Pages: 10-10

    • DOI

      10.1017/qpb.2021.8

    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Journal Article] Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis2021

    • Author(s)
      Susaki Daichi、Suzuki Takamasa、Maruyama Daisuke、Ueda Minako、Higashiyama Tetsuya、Kurihara Daisuke
    • Journal Title

      PLOS Biology

      Volume: 19 Issue: 3 Pages: 3001123-3001123

    • DOI

      10.1371/journal.pbio.3001123

    • Related Report
      2021 Annual Research Report 2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes2020

    • Author(s)
      Kimata Yusuke、Higaki Takumi、Kurihara Daisuke、Ando Naoe、Matsumoto Hikari、Higashiyama Tetsuya、Ueda Minako
    • Journal Title

      Quantitative Plant Biology

      Volume: 1

    • DOI

      10.1017/qpb.2020.4

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A new role for histone demethylases in the maintenance of plant genome integrity2020

    • Author(s)
      Antunez-Sanchez Javier、Naish Matthew、Ramirez-Prado Juan Sebastian、Ohno Sho、Huang Ying、Dawson Alexander、Opassathian Korawit、Manza-Mianza Deborah、Ariel Federico、Raynaud Cecile、Wibowo Anjar、Daron Josquin、Ueda Minako、Latrasse David、Slotkin R Keith、Weigel Detlef、Benhamed Moussa、Gutierrez-Marcos Jose
    • Journal Title

      eLife

      Volume: 9

    • DOI

      10.7554/elife.58533

    • NAID

      120007179207

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Live-Cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana2020

    • Author(s)
      Ueda Minako、Kimata Yusuke、Kurihara Daisuke
    • Journal Title

      Methods in Molecular Biology

      Volume: 2122 Pages: 37-47

    • DOI

      10.1007/978-1-0716-0342-0_4

    • ISBN
      9781071603413, 9781071603420
    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Intracellular dynamics and transcriptional regulations in plant zygotes: a case study of Arabidopsis2020

    • Author(s)
      Kimata Yusuke、Ueda Minako
    • Journal Title

      Plant Reproduction

      Volume: - Issue: 2 Pages: 89-96

    • DOI

      10.1007/s00497-020-00389-7

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Presentation] Live-cell imaging of the polarization dynamics of plant zygote.2021

    • Author(s)
      Ueda M., Kimata Y., Matsumoto H., Higaki T., Komatsu T., Tanaka S., Kurihara D., Higashiyama
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Live imaging of asymmetric cell division of plant zygote.2020

    • Author(s)
      Kimata Y., Higaki T., Kurihara D., Higashiyama T., Ueda M.
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Live-cell imaging from zygote polarization to embryo patterning in plant.2020

    • Author(s)
      Ueda M., Kimata Y., Tanaka S., Higaki T., Kurihara D., Higashiyama T
    • Organizer
      日本発生生物学会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Live-cell imaging of the polarization dynamics of plant zygote: Polar vacuolar distribution is essential for accurate asymmetric division2019

    • Author(s)
      Minako Ueda、Yusuke Kimata、Takehide Kato、Takumi Higaki、Daisuke Kurihara、Shoji Segami、Miyo Terao Morita、Masayoshi Maeshima、Seiichiro Hasezawa、Tetsuya Higashiyama
    • Organizer
      第42回日本分子生物学会年会
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Live-cell imaging of the intracellular dynamics of Arabidopsis zygote2019

    • Author(s)
      Yusuke Kimata、Takumi Higaki、Daisuke Kurihara、Takehide Kato、Shoji Segami、Miyo Terao Morita、Masayoshi Maeshima、Seiichiro Hasezawa、Tetsuya Higashiyama、Minako Ueda
    • Organizer
      Plant Morphodynamics
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Live-cell imaging of the polarization dynamics of Arabidopsis zygote2019

    • Author(s)
      Minako Ueda、Yusuke Kimata、Takumi Higaki、Daisuke Kurihara、Takehide Kato、Shoji Segami、Miyo Terao Morita、Masayoshi Maeshima、Keiko Kuwata、Takamasa Suzuki、Ayato Sato、Moe Yamada、Seiichiro Hasezawa、Tetsuya Higashiyama
    • Organizer
      FASEB Mechanisms in Plant Development meeting
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Live-cell imaging of the polarization dynamics of Arabidopsis zygote2019

    • Author(s)
      Yusuke Kimata、Takehide Kato、Takumi Higaki、Daisuke Kurihara、Tomomi Yamada、Shoji Segami、Miyo Terao Morita、Masayoshi Maeshima、Seiichiro Hasezawa、Keiko Kuwata、Ayato Sato、Takamasa Suzuki、Moe Yamada、Tomokazu Kawashima、Frederic Berger、Yoshikatsu Sato、Masao Tasaka、Tetsuya Higashiyama、Minako Ueda
    • Organizer
      Principles of Pluripotent Stem Cells Underlying Plant Vitality
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 東北大学生命科学研究科植物細胞動態分野

    • URL

      http://www.lifesci.tohoku.ac.jp/PlantCellDyn/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 植物の卵細胞がつくられる様子を生きたまま観察することに成功

    • URL

      https://www.yokohama-cu.ac.jp/news/2020/202103maruyama_PLOSBiol.html

    • Related Report
      2020 Research-status Report
  • [Remarks] 植物の受精卵が非対称にミトコンドリアを分配する仕組みを発見

    • URL

      https://www.lifesci.tohoku.ac.jp/research/results/detail---id-49726.html

    • Related Report
      2020 Research-status Report
  • [Remarks] 名古屋大学トランスフォーマティブ生命分子研究所(植田美那子)

    • URL

      http://www.itbm.nagoya-u.ac.jp/ja/members/m-ueda/

    • Related Report
      2019 Research-status Report

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Published: 2019-07-04   Modified: 2023-01-30  

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