Comprehensive imaging of intracellular dynamics and homemade compounds to elucidate the mechanism of asymmetric division in plants.

• Project/Area Number: 19K22421
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
• Research Institution: Tohoku University (2020-2021); Nagoya University (2019)
• Principal Investigator: Ueda Minako 東北大学, 生命科学研究科, 教授 (20598726)
• Co-Investigator(Kenkyū-buntansha): 金岡 雅浩 名古屋大学, 理学研究科, 講師 (10467277)
• Project Period (FY): 2019-06-28 – 2022-03-31
• Project Status: Completed (Fiscal Year 2021)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2020: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
• Keywords: 植物発生学 / ライブイメージング / 化合物スクリーニング / 植物受精卵 / 植物

Outline of Research at the Start

多細胞生物は複雑な構造をもつが、それら全ては単一細胞である受精卵から発生する。しかし植物では、受精卵の内部でどのような変化が起こるのかや、それを制御する因子など、いまだ全く分かっていない。その理由としては、これまで生きた受精卵を観察できる手法が存在しなかったことと、重要な機能を担う遺伝子の欠損株が得られにくいことが挙げられる。そこで本研究では、まず、研究代表者が開発した受精卵のライブイメージング技術を用いて、受精卵の内部動態を精緻に観察する。加えて、このライブイメージング法と化合物スクリーニングを組み合わせることで、従来法では見出し得なかった鍵因子を網羅的に同定する。

Outline of Final Research Achievements

Polarization and unequal division of zygote are the initial points of ontogeny. In plants, however, it has been largely unknown what factors regulate what events inside the zygote. In this study, we performed comprehensive live imaging in Arabidopsis thaliana and found that the huge vacuoles are essential for the regulation of asymmetric divisions in the zygote (Matsumoto et. al., 2021). Chemical screening also led to the discovery of several novel inhibitors that act on various regulations in plant cell division (in preparation).

Academic Significance and Societal Importance of the Research Achievements

研究代表者らは、植物の受精卵が極性化する動態をリアルタイムで観察することに成功した。さらに、細胞内にある液胞のダイナミックな動きや制御が、受精卵の不等分裂だけでなく、その後の形作りにも必須であることも突き止めた。ほとんどの植物において、受精卵は大きな液胞を持つことから、本研究が明らかにした仕組みは、植物に共通した普遍的な機構であると期待される。また、化合物スクリーニングで得られた阻害剤は、多くの植物種に働くことも判明したので、農業技術への応用など、さまざまな分野への展開が期待できる。

Research Products

• [Int'l Joint Research] Wageningen University(オランダ)
• [Int'l Joint Research] University of Warwick(英国)
• [Int'l Joint Research] Institute of Plant Sciences Paris Saclay(フランス)
• [Int'l Joint Research] Max Planck Institute(ドイツ)
• [Int'l Joint Research] Institute of Plant Sciences Paris Saclay(フランス)
• [Int'l Joint Research] University of Warwick(英国)
• [Journal Article] Dynamic Rearrangement and Directional Migration of Tubular Vacuoles are Required for the Asymmetric Division of the <i>Arabidopsis</i> Zygote (2021)
  • Author(s): Matsumoto Hikari、Kimata Yusuke、Higaki Takumi、Higashiyama Tetsuya、Ueda Minako
  • Journal Title: Plant and Cell Physiology Volume: 62 Issue: 8 Pages: 1280-1289
  • DOI: 10.1093/pcp/pcab075
  • Peer Reviewed / Open Access
• [Journal Article] What is quantitative plant biology? (2021)
  • Author(s): Autran D., Bassel GW., Chae E., Ezer D., Ferjani A., Fleck C., Hamant O., Hartmann FP., Jiao Y., Johnston IG., Kwiatkowska D., Lim BL., Mahnen AP., Morris RJ., Mulder BM., Nakayama N., Sozzani R., Strader LC., Tusscher K., Ueda M., Wolf S.
  • Journal Title: Quantitative Plant Biology Volume: 2 Pages: 10-10
  • DOI: 10.1017/qpb.2021.8
  • Int'l Joint Research
• [Journal Article] Dynamics of the cell fate specifications during female gametophyte development in Arabidopsis (2021)
  • Author(s): Susaki Daichi、Suzuki Takamasa、Maruyama Daisuke、Ueda Minako、Higashiyama Tetsuya、Kurihara Daisuke
  • Journal Title: PLOS Biology Volume: 19 Issue: 3 Pages: 3001123-3001123
  • DOI: 10.1371/journal.pbio.3001123
  • Peer Reviewed / Open Access
• [Journal Article] Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes (2020)
  • Author(s): Kimata Yusuke、Higaki Takumi、Kurihara Daisuke、Ando Naoe、Matsumoto Hikari、Higashiyama Tetsuya、Ueda Minako
  • Journal Title: Quantitative Plant Biology Volume: 1
  • DOI: 10.1017/qpb.2020.4
  • Peer Reviewed / Open Access
• [Journal Article] A new role for histone demethylases in the maintenance of plant genome integrity (2020)
  • Author(s): Antunez-Sanchez Javier、Naish Matthew、Ramirez-Prado Juan Sebastian、Ohno Sho、Huang Ying、Dawson Alexander、Opassathian Korawit、Manza-Mianza Deborah、Ariel Federico、Raynaud Cecile、Wibowo Anjar、Daron Josquin、Ueda Minako、Latrasse David、Slotkin R Keith、Weigel Detlef、Benhamed Moussa、Gutierrez-Marcos Jose
  • Journal Title: eLife Volume: 9
  • DOI: 10.7554/elife.58533
  • NAID: 120007179207
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Live-Cell Imaging of Zygotic Intracellular Structures and Early Embryo Pattern Formation in Arabidopsis thaliana (2020)
  • Author(s): Ueda Minako、Kimata Yusuke、Kurihara Daisuke
  • Journal Title: Methods in Molecular Biology Volume: 2122 Pages: 37-47
  • DOI: 10.1007/978-1-0716-0342-0_4
  • ISBN: 9781071603413, 9781071603420
  • Peer Reviewed
• [Journal Article] Intracellular dynamics and transcriptional regulations in plant zygotes: a case study of Arabidopsis (2020)
  • Author(s): Kimata Yusuke、Ueda Minako
  • Journal Title: Plant Reproduction Volume: - Issue: 2 Pages: 89-96
  • DOI: 10.1007/s00497-020-00389-7
  • Peer Reviewed
• [Presentation] Live-cell imaging of the polarization dynamics of plant zygote. (2021)
  • Author(s): Ueda M., Kimata Y., Matsumoto H., Higaki T., Komatsu T., Tanaka S., Kurihara D., Higashiyama
  • Organizer: 第44回日本分子生物学会年会
  • Int'l Joint Research / Invited
• [Presentation] Live imaging of asymmetric cell division of plant zygote. (2020)
  • Author(s): Kimata Y., Higaki T., Kurihara D., Higashiyama T., Ueda M.
  • Organizer: 第43回日本分子生物学会年会
  • Int'l Joint Research / Invited
• [Presentation] Live-cell imaging from zygote polarization to embryo patterning in plant. (2020)
  • Author(s): Ueda M., Kimata Y., Tanaka S., Higaki T., Kurihara D., Higashiyama T
  • Organizer: 日本発生生物学会
  • Invited
• [Presentation] Live-cell imaging of the polarization dynamics of plant zygote: Polar vacuolar distribution is essential for accurate asymmetric division (2019)
  • Author(s): Minako Ueda、Yusuke Kimata、Takehide Kato、Takumi Higaki、Daisuke Kurihara、Shoji Segami、Miyo Terao Morita、Masayoshi Maeshima、Seiichiro Hasezawa、Tetsuya Higashiyama
  • Organizer: 第42回日本分子生物学会年会
  • Int'l Joint Research / Invited
• [Presentation] Live-cell imaging of the intracellular dynamics of Arabidopsis zygote (2019)
  • Author(s): Yusuke Kimata、Takumi Higaki、Daisuke Kurihara、Takehide Kato、Shoji Segami、Miyo Terao Morita、Masayoshi Maeshima、Seiichiro Hasezawa、Tetsuya Higashiyama、Minako Ueda
  • Organizer: Plant Morphodynamics
  • Int'l Joint Research / Invited
• [Presentation] Live-cell imaging of the polarization dynamics of Arabidopsis zygote (2019)
  • Author(s): Minako Ueda、Yusuke Kimata、Takumi Higaki、Daisuke Kurihara、Takehide Kato、Shoji Segami、Miyo Terao Morita、Masayoshi Maeshima、Keiko Kuwata、Takamasa Suzuki、Ayato Sato、Moe Yamada、Seiichiro Hasezawa、Tetsuya Higashiyama
  • Organizer: FASEB Mechanisms in Plant Development meeting
  • Int'l Joint Research / Invited
• [Presentation] Live-cell imaging of the polarization dynamics of Arabidopsis zygote (2019)
  • Author(s): Yusuke Kimata、Takehide Kato、Takumi Higaki、Daisuke Kurihara、Tomomi Yamada、Shoji Segami、Miyo Terao Morita、Masayoshi Maeshima、Seiichiro Hasezawa、Keiko Kuwata、Ayato Sato、Takamasa Suzuki、Moe Yamada、Tomokazu Kawashima、Frederic Berger、Yoshikatsu Sato、Masao Tasaka、Tetsuya Higashiyama、Minako Ueda
  • Organizer: Principles of Pluripotent Stem Cells Underlying Plant Vitality
  • Int'l Joint Research / Invited
• [Remarks] 東北大学生命科学研究科植物細胞動態分野
  • URL: http://www.lifesci.tohoku.ac.jp/PlantCellDyn/
• [Remarks] 植物の卵細胞がつくられる様子を生きたまま観察することに成功
  • URL: https://www.yokohama-cu.ac.jp/news/2020/202103maruyama_PLOSBiol.html
• [Remarks] 植物の受精卵が非対称にミトコンドリアを分配する仕組みを発見
  • URL: https://www.lifesci.tohoku.ac.jp/research/results/detail---id-49726.html
• [Remarks] 名古屋大学トランスフォーマティブ生命分子研究所（植田美那子）
  • URL: http://www.itbm.nagoya-u.ac.jp/ja/members/m-ueda/