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Development of ultra-high resolution infrared spectrum microscope

Research Project

Project/Area Number 19K22442
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 44:Biology at cellular to organismal levels, and related fields
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Ogura Toshihiko  国立研究開発法人産業技術総合研究所, 生命工学領域, 上級主任研究員 (70371028)

Co-Investigator(Kenkyū-buntansha) 山本 条太郎  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (20585088)
Project Period (FY) 2019-06-28 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2019: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords窒化シリコン薄膜 / 走査電子顕微鏡 / 赤外線 / インピーダンス / 窒化シリコン / 金属薄膜層 / 走査電子 / ナノ粒子 / 生物試料 / 波長分光
Outline of Research at the Start

本研究提案では、水溶液中の生物試料やナノ粒子をそのままの状態で観察し分析を行なう、新たな原理に基づく赤外線観察・分析システム(走査電子赤外線分光観察法)を開発する。本装置を用いることで、セラミック材料や有機材料、食品や化粧品等のナノ粒子の観察と組成分析、さらには環境中のナノレベルの汚染物質の解析にも応用を勧める。さらに、生きた生物試料の高分解能の赤外線観察を目指す予定である。

Outline of Final Research Achievements

In this study, a scanning electron infrared microscope was developed using infrared and other radiation emitted by scanning electron beams incident on a thin film. As the inside of the scanning electron microscope is a vacuum, it is essential to encapsulate the sample with a highly pressure-resistant thin film. In this study, a biological sample or organic material is sealed between two silicon nitride thin films, and electron beams are injected into the upper part of the thin films while scanning. This creates a local heat spot at the electron beam irradiation position, from which various physical rays, including infrared rays, are emitted. These physical rays are transmitted through the sample and then detected to produce an image. This system enables observation of organic materials, etc., and also enables analysis of thermal impedance changes.

Academic Significance and Societal Importance of the Research Achievements

本研究では、生物試料や有機材料等の高分解能での観察を行うため、薄膜上部の金属層に電子線を走査させながら入射し、ここから生じる赤外線等を試料を透過させた後に検出し、画像化する新たな顕微鏡システムを開発した。これにより、電子線を直接試料に照射すること無く、電子線から変換されたより低ダメージの物理線で観察を行うため、極めて低いダメージで観察が可能である。さらに、電子線の直接照射に比べてコントラストが高く、スペクトル成分の分析も可能とした。そのため、本観察システムでは、溶液中の生物試料や有機材料、ナノ粒子等の直接観察と分析が可能となり、広範囲の産業分野への貢献が期待される。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (13 results)

All 2022 2021 2020 2019

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 7 results) Presentation (6 results) (of which Int'l Joint Research: 3 results,  Invited: 6 results)

  • [Journal Article] Development of multi-frequency impedance scanning electron microscopy2022

    • Author(s)
      Ogura Toshihiko
    • Journal Title

      PLOS ONE

      Volume: 17 Issue: 1 Pages: e0263098-e0263098

    • DOI

      10.1371/journal.pone.0263098

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Raman scattering enhancement of dielectric microspheres on silicon nitride film2022

    • Author(s)
      Ogura Toshihiko
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1

    • DOI

      10.1038/s41598-022-09315-5

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nanoscale observation of PM2.5 incorporated into mammalian cells using scanning electron-assisted dielectric microscope2021

    • Author(s)
      Okada Tomoko、Iwayama Tomoaki、Murakami Shinya、Torimura Masaki、Ogura Toshihiko
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 228-228

    • DOI

      10.1038/s41598-020-80546-0

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Scanning Electron-Assisted Dielectric Microscopy Reveals Autophagosome Formation by LC3 and ATG12 in Cultured Mammalian Cells2021

    • Author(s)
      Okada Tomoko、Ogura Toshihiko
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 22 Issue: 4 Pages: 1834-1834

    • DOI

      10.3390/ijms22041834

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy2019

    • Author(s)
      Ogura Toshihiko
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 8 Pages: e0221296-e0221296

    • DOI

      10.1371/journal.pone.0221296

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of INSOL‐tag for proteome‐wide protein handling and its application in protein array analysis2019

    • Author(s)
      Fukuda Eriko、Mori Masatoshi、Shiku Hiroshi、Miyahara Yoshihiro、Kawamura Yoshifumi、Ogawa Koji、Ogura Toshihiko、Goshima Naoki
    • Journal Title

      Genes to Cells

      Volume: 25 Issue: 1 Pages: 41-53

    • DOI

      10.1111/gtc.12735

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Osteoblastic lysosome plays a central role in mineralization2019

    • Author(s)
      Iwayama Tomoaki、Okada Tomoko、Ueda Tsugumi、Tomita Kiwako、Matsumoto Shuji、Takedachi Masahide、Wakisaka Satoshi、Noda Takeshi、Ogura Taku、Okano Tomomichi、Fratzl Peter、Ogura Toshihiko、Murakami Shinya
    • Journal Title

      Science Advances

      Volume: 5 Issue: 7

    • DOI

      10.1126/sciadv.aax0672

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Direct observation of biological fine particles in water by scanning-electron assisted dielectric microscopy2021

    • Author(s)
      小椋俊彦、岡田知子
    • Organizer
      Pacifichem 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-resolution imaging of proteins and cells under aqueous condition using scanning-electron assisted dielectric microscopy2020

    • Author(s)
      小椋 俊彦、伊藤 徹次、岡田 知子
    • Organizer
      第30回日本MRS年次大会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] 革新的液中ナノ顕微鏡開発と細胞外微粒子の包括的解明2020

    • Author(s)
      小椋 俊彦
    • Organizer
      日本分析化学会第69回年会
    • Related Report
      2020 Research-status Report
    • Invited
  • [Presentation] Nanoscale observation of biological specimens in aqueous condition by scanning-electron assisted dielectric microscopy2019

    • Author(s)
      小椋 俊彦、岡田 知子
    • Organizer
      OKINAWA COLLOIDS 2019 conference
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Nanoscale imaging of unstained biological specimens in water using newly developed scanning-electron assisted dielectric microscopy2019

    • Author(s)
      小椋 俊彦、岡田 知子
    • Organizer
      5th International Kyushu Colloid Colloquium
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 走査電子誘電率顕微鏡によるナノ・マイクロプラスチックの細胞への影響解析2019

    • Author(s)
      小椋 俊彦、岡田 知子
    • Organizer
      産総研・シンポジウム「マイクロプラスチックの計測・評価を考える」
    • Related Report
      2019 Research-status Report
    • Invited

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Published: 2019-07-04   Modified: 2023-01-30  

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