Spatiotemporal analysis and pathogenesis of proinflammatory regulatory T cells in intestinal villi
Project/Area Number |
19K22624
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Research Category |
Grant-in-Aid for Challenging Research (Exploratory)
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Allocation Type | Multi-year Fund |
Review Section |
Medium-sized Section 53:Organ-based internal medicine and related fields
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Research Institution | Keio University |
Principal Investigator |
SUJINO Tomohisa 慶應義塾大学, 医学部(信濃町), 講師 (40464862)
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Project Period (FY) |
2019-06-28 – 2021-03-31
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Project Status |
Completed (Fiscal Year 2020)
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Budget Amount *help |
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2020: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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Keywords | 2光子顕微鏡 / 腸管免疫 / 制御性T細胞 / 炎症性腸疾患 / 2光子顕微鏡 |
Outline of Research at the Start |
腸管のホメオスタシスの維持には自然リンパ球、マクロファージ、制御性T細胞(regulatory T cells; Treg)などの関与が報告されている。特に炎症抑制能力を有しているTregは消化管の炎症抑制の中心的役割を担っており、炎症の収束及び腸管上皮再生のキープレーヤーである。しかし、炎症性腸疾患患者において、Tregは増加しているにもかかわらず、腸炎が抑制できない相反する結果を有している。腸管炎症において腸管の上皮細胞障害(炎症期)およびその回復期のTreg集積動態に着目し、Treg動態不全と慢性大腸炎発症機序解明について研究を行う。
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Outline of Final Research Achievements |
The dynamics and functional regulation of Treg in the intestine at the single cell level is unknown. It has been reported that the number of Treg in the mucosa is increased in patients with ulcerative colitis compared with that in healthy intestine. On the other hand, chronicity of intestinal inflammation and insufficient epithelial repair are shown in this patient. In order to analyze the dynamics of Treg in gastrointestinal diseases, this study used IVM to investigate the localization of Treg in the intestinal mucosa during inflammation in an in vivo model. In this study, we used IVM to examine the frequency and dynamics of intramucosal localization of Treg (between the mucosal lamina propria and intestinal epithelium) and mucosal lamina propria-epithelium cross-migration during inflammation in an in vivo model.
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Academic Significance and Societal Importance of the Research Achievements |
消化管内における1つの細胞がどこにいて、どのように動きを制御されているかについてはいままで不明であった。腸管の一つ一つの細胞がどこに存在しどのように動いているのかを可視化することに成功した。人の病気でも同様に可視化することが可能であり、局在、動態の違いから病気の原因解明につながる可能性がある。
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Significance of endoscopic deep small bowel evaluation using balloon-assisted enteroscopy for Crohn's disease in clinical remission2021
Author(s)
Takabayashi K, Hosoe N, Kato M, Hayashi Y, Nanki K, Fukuhara K, Mikami Y, Mizuno S, Sujino T, Mutaguchi M, Naganuma M, Yahagi N, Ogata H, Kanai T.
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Journal Title
J Gastroenterol.
Volume: 56(1)
Issue: 1
Pages: 25-33
DOI
Related Report
Peer Reviewed
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[Journal Article] Characteristics of the Mucosal Surface on Scanning Electron Microscopy in Patients with Remitting Ulcerative Colitis2021
Author(s)
Nomura E, Sujino T, Hosoe N, Yoshimatsu Y, Tanemoto S, Takabayashi K, Mutaguchi M, Shimoda M, Naganuma M, Ogata H, Kanai T.
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Journal Title
Dig Dis Sci.
Volume: -
Issue: 9
Pages: 1-6
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Clinical, Endoscopic, and Pathological Characteristics of Immune Checkpoint Inhibitor-Induced Gastroenterocolitis2021
Author(s)
Hayashi Y, Hosoe N, Takabayashi K, Limpias Kamiya KJL, Tsugaru K, Shimozaki K, Hirata K, Fukuhara K, Fukuhara S, Mutaguchi M, Sujino T, Sukawa Y, Hamamoto Y, Naganuma M, Takaishi H, Shimoda M, Ogata H, Kanai T.
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Journal Title
Dig Dis Sci.
Volume: -
Pages: 1-6
DOI
Related Report
Peer Reviewed
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[Journal Article] The liver-brain-gut neural arc maintains the Treg cell niche in the gut2020
Author(s)
Teratani T, Mikami Y, Nakamoto N, Suzuki T, Harada Y, Okabayashi K, Hagihara Y, Taniki N, Kohno K, Sibata S, Miyamoto K, Ishigame H, Chu Po-Sung, Sujino T, Suda W, Hattori M, Matsui M, Okada T, Okano H, Inoue M, Yada T, Kitagawa Y, Yoshimura A, Tanida M, Tsuda M, Iwasaki Y, Kanai T.
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Journal Title
Nature
Volume: 585
Issue: 7826
Pages: 591-596
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35.2019
Author(s)
Koda Y, Nakamoto N, Chu P, Ugamura A, Teratani T, Shiba S, Taniki N, Sujino T, Miyamoto K, Mikami Y, Suzuki T, Yamaguchi A, Morikawa R, Sato K, Yoshimoto T, Kanai K.
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Journal Title
J Clin Invest
Volume: 129(8)
Issue: 8
Pages: 3201-3213
DOI
Related Report
Peer Reviewed / Open Access
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