骨軟部腫瘍の「小胞体ストレス」機能解析に基づいた新規治療法開発

• Project/Area Number: 19K22694
• Research Category: Grant-in-Aid for Challenging Research (Exploratory)
• Allocation Type: Multi-year Fund
• Review Section: Medium-sized Section 56:Surgery related to the biological and sensory functions and related fields
• Research Institution: Juntendo University
• Principal Investigator: 末原 義之 順天堂大学, 医学部, 客員教授 (70509405)
• Co-Investigator(Kenkyū-buntansha): 高阪 真路 国立研究開発法人国立がん研究センター, 研究所, 分野長 (00627119) ; 小林 英介 国立研究開発法人国立がん研究センター, 中央病院, 医長 (40365292) ; 北野 滋久 公益財団法人がん研究会, 有明病院 がん免疫治療開発部, 部長 (60402682) ; 林 大久生 順天堂大学, 医学部, 准教授 (70569128) ; 齋藤 剛 順天堂大学, 医学部, 准教授 (80439736)
• Project Period (FY): 2019-06-28 – 2024-03-31
• Project Status: Granted (Fiscal Year 2022)
• Budget Amount: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2020: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2019: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 骨軟部腫瘍 / 骨軟部肉腫 / 小胞体ストレス

Outline of Research at the Start

骨軟部肉腫の治療成績は依然極めて不良であり、希少がんであるが故に新規治療の開発が滞っている。肉腫の特徴である組織特異的融合遺伝子関連因子タンパク質発現データベースの構築し、特に小胞体ストレスパスウェイの一つIRE1/XBP1に注目し研究を進めたユーイング肉腫においてその阻害剤が腫瘍増殖を著しく阻害することを解明している。以上を元に現在まで全く不明である肉腫と小胞体ストレスの機能解明の基に、骨軟部腫瘍組織型横断的な新規治療法開発を進める。

Outline of Annual Research Achievements

骨軟部肉腫の治療成績は依然極めて不良であり、希少がんであるが故に新規治療の開発が滞っている。肉腫の特徴である組織特異的融合遺伝子 関連因子タンパク質発現データベースの構築し、特に小胞体ストレスパスウェイの一つIRE1/XBP1に注目し研究を進めたユーイング肉腫においてその阻害剤が腫瘍増殖を著しく阻害することを解明している。以上を元に現在まで全く不明である肉腫と小胞体ストレスの機能解明の基に、 骨軟部腫瘍組織型横断的な新規治療法開発を進めている。本研究は治療成績が依然極めて不良である骨軟部腫瘍において新規治療法開発の基に予後改善を目指す。具体的には組織型特異的融合遺伝子に 基づいた整備されたタンパク質発現データベースを用い研究を進め、特にそのデータベース活用により発見された小胞体ストレスの各種パスウェイに注目し、骨軟部腫瘍における機能・腫瘍増殖・阻害剤効果などの面より解明を進めている。また近年の他癌種において腫瘍細胞内/免疫細胞 内(樹状細胞/T細胞)の小胞体ストレス応答が、発癌・癌増殖と関連付けられていることを起点に免疫面よりも解明を進めている。以上より現在まで不明である骨軟部腫瘍と小胞体ストレスの生物学的機能を解明し、それを基に新規治療法開発を進め、骨軟部腫瘍生命予後の改善に挑んでいる。

Current Status of Research Progress

3: Progress in research has been slightly delayed.

Reason

小胞体ストレスパスウェイ解析にて複数の解析価値あるパスウェイが同定可能であったため機能解析の再計画を行った。

Strategy for Future Research Activity

昨年度に引き続き、(A-1)骨軟部腫瘍の小胞体ストレスパスウェイ発現検証として、骨軟部腫瘍組織型それぞれの腫瘍検体及び細胞株にて小胞体ストレスパスウェ イ発現を確認 し、臨床病理学的因子解析を進め、骨軟部腫瘍での小胞体ストレス発現解明とデータベース化を行う。(A-2) 小胞体ストレスパス ウェイの機能解析として各組 織型腫瘍細胞株で各パスウェイを発現制御、各阻害剤を用いた抗腫瘍効果を検証する。(A-3) 阻害剤のin vivo解析を進め実臨床試験へ準備を進める。 特に(A-4) 遺伝子発現・遺伝 子変異解析とネットワーク解析として、小胞体 ストレスパスウェイの機能解析で腫瘍抑制効果を認めた標的遺伝子の制御細胞株にて網羅的遺伝子発現・遺伝子変異解析、ゲノムワイドに機能解明を行う。

Research Products

• [Int'l Joint Research] Memorial Sloan Kettering Cancer Center(米国)
• [Journal Article] Dedifferentiated liposarcoma in the extremity and trunk wall: A multi-institutional study of 132 cases by the Japanese Musculoskeletal Oncology Group (JMOG) (2023)
  • Author(s): Morii Takeshi、Anazawa Ukei、Sato Chiaki、Iwata Shintaro、Nakagawa Makoto、Endo Makoto、Nakamura Tomoki、Ikuta Kunihiro、Nishida Yoshihiro、Nakayama Robert、Udaka Toru、Kawamoto Teruya、Kito Munehisa、Sato Kenji、Imanishi Jungo、Akiyama Toru、Kobayashi Hiroshi、NKawano Hirotaka、Ueda Takafumi、Morioka Hideo、et al.
  • Journal Title: European Journal of Surgical Oncology Volume: 49 Issue: 2 Pages: 353-361
  • DOI: 10.1016/j.ejso.2022.08.024
  • Peer Reviewed / Open Access
• [Journal Article] Clinical relevance of PD-L2 expression in surgically resected lung adenocarcinoma. (2022)
  • Author(s): Takamochi K, Hara K, Hayashi T, Kohsaka S, Takahashi F, Suehara Y, Shimokawa M, Suzuki K.
  • Journal Title: Lung Cancer Volume: 168 Pages: 50-58
  • DOI: 10.1016/j.lungcan.2022.04.011
  • Peer Reviewed / Open Access
• [Journal Article] Molecular and clinicopathological analysis revealed an immuno-checkpoint inhibitor as a potential therapeutic target in a subset of high-grade myxofibrosarcoma (2022)
  • Author(s): Yamashita Atsushi、Suehara Yoshiyuki、Hayashi Takuo、Takagi Tatsuya、Kubota Daisuke、Sasa Keita、Hasegawa Nobuhiko、Ishijima Muneaki、Yao Takashi、Saito Tsuyoshi
  • Journal Title: Virchows Archiv Volume: 481 Issue: 4 Pages: 1-17
  • DOI: 10.1007/s00428-022-03358-9
  • Peer Reviewed / Open Access
• [Journal Article] Perioperative Adriamycin plus ifosfamide vs. gemcitabine plus docetaxel for high-risk soft tissue sarcomas: randomised, phase II/III study JCOG1306 (2022)
  • Author(s): Tanaka Kazuhiro、Machida Ryunosuke、Kawai Akira、Nakayama Robert、Tsukushi Satoshi、et al.
  • Journal Title: British Journal of Cancer Volume: 127 Issue: 8 Pages: 1487-1496
  • DOI: 10.1038/s41416-022-01912-5
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of Rapid and Accurate Screening System for Molecular Target Therapy of Osteosarcoma (2022)
  • Author(s): Sasa Keita、Saito Tsuyoshi、Kurihara Taisei、Hasegawa Nobuhiko、Sano Kei、Kubota Daisuke、Akaike Keisuke、Okubo Taketo、Hayashi Takuo、Takagi Tatsuya、Ishijima Muneaki、Suehara Yoshiyuki
  • Journal Title: Technology in Cancer Research & Treatment Volume: 21 Pages: 15-15
  • DOI: 10.1177/15330338221138217
  • Peer Reviewed / Open Access
• [Journal Article] Correction to: A case of primary distal-type epithelioid sarcoma of the lumbar vertebra with a review of literature (2021)
  • Author(s): Ura Ayako、Saito Tsuyoshi、Motoi Toru、Takagi Tatsuya、Suehara Yoshiyuki、Kurihara Taisei、Sano Kei、Sasa Keita、Hayashi Takuo、Yao Takashi
  • Journal Title: Virchows Archiv Volume: 478 Issue: 3 Pages: 613-613
  • DOI: 10.1007/s00428-020-02970-x
  • Peer Reviewed / Open Access
• [Journal Article] Histological characteristics of lung adenocarcinoma with uncommon actionable alterations: special emphasis on MET exon 14 skipping alterations (2021)
  • Author(s): Hayashi Takuo、Kohsaka Shinji、Takamochi Kazuya、Kishikawa Satsuki、Ikarashi Daiki、Sano Kei、Hara Kieko、Onagi Hiroko、Suehara Yoshiyuki、Takahashi Fumiyuki、Saito Tsuyoshi、Nakatsura Tetsuya、Kitano Shigehisa、Suzuki Kenji、Yao Takashi
  • Journal Title: Histopathology Volume: - Issue: 7 Pages: 1-1
  • DOI: 10.1111/his.14311
  • Peer Reviewed / Open Access
• [Journal Article] Comprehensive molecular and clinicopathological profiling of desmoid tumours (2021)
  • Author(s): Kohsaka Shinji、Hirata Makoto、Ikegami Masachika、Ueno Toshihide、Kojima Shinya、Sakai Tomohisa、Ito Kan、Naka Norifumi、Ogura Koichi、Kawai Akira、Iwata Shintaro、Okuma Tomotake、Yonemoto Tsukasa、Kobayashi Hiroshi、Suehara Yoshiyuki、Hiraga Hiroaki、Kawamoto Teruya、Motoi Toru、Oda Yoshinao、Matsubara Daisuke、etc
  • Journal Title: European Journal of Cancer Volume: 145 Pages: 109-120
  • DOI: 10.1016/j.ejca.2020.12.001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Immunological status of peripheral blood is associated with prognosis in patients with bone and soft-tissue sarcoma (2021)
  • Author(s): Youngji Kim
  • Journal Title: Oncology letters Volume: 21 Issue: 3 Pages: 212-212
  • DOI: 10.3892/ol.2021.12473
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Establishment of a patient-derived xenograft mouse model of pleomorphic leiomyosarcoma (2021)
  • Author(s): Shimada Yasuhiro、Naito Tomoharu、Hayashi Takuo、Saito Tsuyoshi、Suehara Yoshiyuki、Kakinuma Chihaya、Nozaki Yuji、Takagi Hisayoshi、Yao Takashi
  • Journal Title: Journal of Toxicologic Pathology Volume: 34 Issue: 1 Pages: 89-93
  • DOI: 10.1293/tox.2020-0061
  • NAID: 130007982485
  • ISSN: 0914-9198, 1881-915X
  • Peer Reviewed / Open Access
• [Journal Article] NTRK fusion in Japanese colorectal adenocarcinomas (2021)
  • Author(s): Yamashiro Y.、Kurihara T.、Hayashi T.、Suehara Y.、Yao T.、Kato S.、Saito T.
  • Journal Title: Scientific Reports Volume: 11 Issue: 1 Pages: 5635-5635
  • DOI: 10.1038/s41598-021-85075-y
  • Peer Reviewed / Open Access
• [Journal Article] Transcription start site-level expression of thyroid transcription factor 1 isoforms in lung adenocarcinoma and its clinicopathological significance. (2021)
  • Author(s): Sano K, Hayashi T, Suehara Y, Hosoya M, Takamochi K, Kohsaka S, Kishikawa S, Kishi M, Saito S, Takahashi F, Kaneko K, Suzuki K, Yao T, Ishijima M, Saito T.
  • Journal Title: J Pathol Clin Res Volume: 7 Issue: 4 Pages: 361-374
  • DOI: 10.1002/cjp2.213
  • Peer Reviewed / Open Access
• [Journal Article] Highly sensitive fusion detection using plasma cell-free RNA in non-small-cell lung cancers. (2021)
  • Author(s): Hasegawa N, Kohsaka S, Kurokawa K, Shinno Y, Takeda Nakamura I, Ueno T, Kojima S, Kawazu M, Suehara Y, Ishijima M, Goto Y, Kojima Y, Yonemori K, Hayashi T, Saito T, Shukuya T, Takahashi F, Takahashi K, Mano H.
  • Journal Title: Cancer Sci Volume: 112 Issue: 10 Pages: 4393-4403
  • DOI: 10.1111/cas.15084
  • Peer Reviewed / Open Access
• [Journal Article] iPSC-Derived Neoantigen-Specific CTL Therapy for Ewing Sarcoma (2021)
  • Author(s): Ishii Midori、Ando Jun、Yamazaki Satoshi、Toyota Tokuko、Ohara Kazuo、Furukawa Yoshiki、Suehara Yoshiyuki、Nakanishi Mahito、Nakashima Kazutaka、Ohshima Koichi、Nakauchi Hiromitsu、Ando Miki
  • Journal Title: Cancer Immunology Research Volume: 9 Issue: 10 Pages: 1175-1186
  • DOI: 10.1158/2326-6066.cir-21-0193
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Programmed death-ligand 1 expression and its associations with clinicopathological features, prognosis, and driver oncogene alterations in surgically resected lung adenocarcinoma (2021)
  • Author(s): Takamochi K, Hara K, Hayashi T, Kohsaka S, Takahashi F, Suehara Y, Suzuki K.
  • Journal Title: Lung Cancer Volume: 161 Pages: 163-170
  • DOI: 10.1016/j.lungcan.2021.09.011
  • Peer Reviewed / Open Access
• [Journal Article] Nodular fasciitis adjacent to the left humerus in a 15-year-old boy (2021)
  • Author(s): Sasa Keita、Suehara Yoshiyuki、Takagi Tatsuya、Sano Kei、Hayashi Takuo、Saito Tsuyoshi
  • Journal Title: Human Pathology: Case Reports Volume: 23 Pages: 200478-200478
  • DOI: 10.1016/j.ehpc.2021.200478
  • Peer Reviewed / Open Access
• [Journal Article] Symptomatic Venous Thromboembolism in Patients with Malignant Bone and Soft Tissue Tumors: A Prospective Multicenter Cohort Study (2020)
  • Author(s): Iwata Shintaro、Japanese Musculoskeletal Oncology Group (JMOG)、Kawai Akira、Ueda Takafumi、Ishii Takeshi
  • Journal Title: Annals of Surgical Oncology Volume: - Issue: 7 Pages: 3919-3927
  • DOI: 10.1245/s10434-020-09308-6
  • Peer Reviewed / Open Access
• [Journal Article] Identification of a Novel MAN1A1-ROS1 Fusion Gene Through mRNA-based Screening for Tyrosine Kinase Gene Aberrations in a Patient with Leiomyosarcoma (2020)
  • Author(s): Suehara Y, Kohsaka S, Hayashi T, Akaike K, Kurisaki-Arakawa A, Sato S, Kobayashi E, Mizuno S, Ueno T, Morii T, Okuma T, Kurihara T, Hasegawa N, Sano K, Sasa K, Okubo T, Kim Y, Mano H, Saito T
  • Journal Title: Clin Orthop Relat Res Volume: － Issue: 4 Pages: 838-852
  • DOI: 10.1097/corr.0000000000001548
  • Peer Reviewed / Open Access
• [Journal Article] Diffuse expression of MUC6 defines a distinct clinicopathological subset of pulmonary invasive mucinous adenocarcinoma (2020)
  • Author(s): Kishikawa S, Hayashi T, Saito T, Takamochi K, Kohsaka S, Sano K, Sasahara N, Sasa K, Kurihara T, Hara K, Suehara Y, Takahashi F, Suzuki K, Yao T
  • Journal Title: Mod Pathol Volume: － Issue: 4 Pages: 786-797
  • DOI: 10.1038/s41379-020-00690-w
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of multiple novel patient-derived models of desmoplastic small round cell tumor enabling functional characterization of ERBB pathway signaling and pre-clinical evaluation of a novel targeted therapy approach (2020)
  • Author(s): Smith Roger S.、Odintsov Igor、Liu Zebing、Suehara Yoshiyuki、Shaw Melissa、Bui Gabrielle、Hartono Alifiani B.、Gladstone Eric、Kunte Siddharth、Magnan Heather、Khodos Inna、De Stanchina Elisa、La Quaglia Michael P.、Yao Jinjuan、Lae Marick、Lee Sean B.、Spraggon Lee、Pratilas Christine A.、Ladanyi Marc、Somwar Romel
  • Journal Title: BioRxiv Volume: 2020 Pages: 308940-308940
  • DOI: 10.1101/2020.09.22.308940
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A case of primary distal-type epithelioid sarcoma of the lumbar vertebra with a review of literature (2020)
  • Author(s): Ura Ayako、Saito Tsuyoshi、Motoi Toru、Takagi Tatsuya、Suehara Yoshiyuki、Kurihara Taisei、Sano Kei、Sasa Keita、Hayashi Takuo、Yao Takashi
  • Journal Title: Virchows Archiv Volume: - Issue: 2 Pages: 1-1
  • DOI: 10.1007/s00428-020-02955-w
  • Peer Reviewed
• [Journal Article] Nanostring-based screening for tyrosine kinase fusions in inflammatory myofibroblastic tumors (2020)
  • Author(s): Kurihara Taisei、Suehara Yoshiyuki、Akaike Keisuke、Hayashi Takuo、Kohsaka Shinji、Ueno Toshihide、Hasegawa Nobuhiko、Takagi Tatsuya、Sasa Keita、Okubo Taketo、Kim Youngji、Mano Hiroyuki、Yao Takashi、Kaneko Kazuo、Saito Tsuyoshi
  • Journal Title: Scientific Reports Volume: 10 Issue: 1 Pages: 1-1
  • DOI: 10.1038/s41598-020-75596-3
  • Peer Reviewed / Open Access
• [Journal Article] Comprehensive clinicopathological and molecular analysis of primary malignant melanoma of the oesophagus (2020)
  • Author(s): Tsuyama Sho、Kohsaka Shinji、Hayashi Takuo、Suehara Yoshiyuki、Hashimoto Takashi、Kajiyama Yoshiaki、Tsurumaru Masahiko、Ueno Toshihide、Mano Hiroyuki、Yao Takashi、Saito Tsuyoshi
  • Journal Title: Histopathology Volume: 78 Issue: 2 Pages: 240-251
  • DOI: 10.1111/his.14210
  • Peer Reviewed
• [Journal Article] Clinicopathological characteristics of lung adenocarcinoma with compound EGFR mutations. (2020)
  • Author(s): Hayashi T, Kohsaka S, Takamochi K, Hara K, Kishikawa S, Sano K, Takahashi F, Suehara Y, Saito T, Takahashi K, Suzuki K, Yao T.
  • Journal Title: Human Pathology Volume: 14 Pages: 42-51
  • DOI: 10.1016/j.humpath.2020.07.007
  • Peer Reviewed
• [Journal Article] Molecular and clinicopathological features of colorectal adenocarcinoma with enteroblastic differentiation. (2020)
  • Author(s): 3.Yamashiro Y, Saito T, Hayashi T, Murakami T, Yanai Y, Tsuyama S, Suehara Y, Takamochi K, Yao T.
  • Journal Title: Histopathol Volume: in press Issue: 3 Pages: 492-502
  • DOI: 10.1111/his.14158
  • Peer Reviewed
• [Journal Article] Preoperative denosumab treatment with curettage may be a risk factor for recurrence of giant cell tumor of bone (2020)
  • Author(s): Sano Kei、Suehara Yoshiyuki、Okubo Taketo、Sasa Keita、Kurihara Taisei、Akaike Keisuke、Kubota Daisuke、Torigoe Tomoaki、Hasegawa Nobuhiko、Ishii Midori、Nakamura Yasuhiro、Kim Youngji、Takagi Tatsuya、Kaneko Kazuo、Hayashi Takuo、Saito Tsuyoshi
  • Journal Title: Journal of Orthopaedic Surgery Volume: 28 Issue: 2 Pages: 1-1
  • DOI: 10.1177/2309499020929786
  • Peer Reviewed / Open Access
• [Journal Article] Transformation from EGFR/PTEN co-mutated lung adenocarcinoma to small cell carcinoma in lymph node metastasis (2020)
  • Author(s): Hayashi T, Takamochi K, Kohsaka S, Kishikawa S, Suehara Y, Takahashi F, Suzuki K, Saito T, Yao T
  • Journal Title: Pathology International Volume: － Issue: 5 Pages: 295-299
  • DOI: 10.1111/pin.12919
  • Peer Reviewed / Open Access
• [Journal Article] Assessment of Predictive Biomarkers of the Response to Pazopanib Based on an Integrative Analysis of High-grade Soft-tissue Sarcomas: Analysis of a Tumor Sample from a Responder and Patients with Other Soft-tissue Sarcomas (2020)
  • Author(s): Suehara Y.、Kohsaka S.、Yamaguchi S.、Hayashi T.、Kurihara T.、Sano K.、Sasa K.、Akaike K.、Ueno T.、Kojima S.、Ikegami M.、Mizuno S.、Okubo T.、Kim Y.、Kaneko K.、Saito T.、Kato S.、Mano H.
  • Journal Title: Clinical Orthopaedics & Related Research Volume: 478 Issue: 11 Pages: 2461-2476
  • DOI: 10.1097/corr.0000000000001322
  • Peer Reviewed
• [Journal Article] Identification of Novel CD74-NRG2α Fusion From Comprehensive Profiling of Lung Adenocarcinoma in Japanese Never or Light Smokers (2020)
  • Author(s): Kohsaka S, Hayashi T, Nagano M, Ueno T, Kojima S, Kawazu M, Shiraishi Y, Kishikawa S, Suehara Y, Takahashi F, Takahashi K, Suzuki K, Takamochi K, Mano H
  • Journal Title: Journal of Thoracic Oncology Volume: － Issue: 6 Pages: 30098-8
  • DOI: 10.1016/j.jtho.2020.01.021
  • Peer Reviewed / Open Access
• [Journal Article] Nintedanib inhibits epithelial-mesenchymal transition in A549 alveolar epithelial cells through regulation of the TGF-β/Smad pathway (2020)
  • Author(s): Ihara Hiroaki、Mitsuishi Yoichiro、Kato Motoyasu、Takahashi Fumiyuki、Tajima Ken、Hayashi Takuo、Takamochi Kazuya、Suehara Yoshiyuki、Ogura Dai、Niwa Shin-ichiro、Suzuki Kenji、Takahashi Kazuhisa
  • Journal Title: Respiratory Investigation Volume: 58 Issue: 4 Pages: 275-284
  • DOI: 10.1016/j.resinv.2020.01.003
  • Peer Reviewed
• [Journal Article] Activation of insulin-like growth factor-1 receptor confers acquired resistance to osimertinib in non-small cell lung cancer with EGFR T790M mutation (2020)
  • Author(s): Hayakawa D, Takahashi F, Mitsuishi Y, Tajima K, Hidayat M, Winardi W, Ihara H, Kanamori K, Matsumoto N, Asao T, Ko R, Shukuya T, Takamochi K, Hayashi T, Suehara Y, Takeda Nakamura I, Ueno T, Kohsaka S, Mano H, Takahashi K
  • Journal Title: Thorac Cancer Volume: 11 Issue: 1 Pages: 140-149
  • DOI: 10.1111/1759-7714.13255
  • Peer Reviewed / Open Access
• [Journal Article] The role of chemotherapy and radiotherapy in localized extraskeletal osteosarcoma (2020)
  • Author(s): Heng Marilyn、Gupta Abha、Chung Peter W、Bishop Andrew J.、Ueda Takafumi、Kakunaga Shigeki、Kawai Akira、Sugiura Hideshi、Kidani Teruki、Nagano Akihito、Yamamoto Norio、Tsuchiya Hiroyuki、Yanagawa Takashi、Morioka Hideo、Simose Shoji、Yamagami Yoshiki、Yamamoto Tetsuji、Kawasaki Motohiro、Torigoe Tomoaki .et.al
  • Journal Title: European Journal of Cancer Volume: 125 Pages: 130-141
  • DOI: 10.1016/j.ejca.2019.07.029
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Clinical genomic sequencing of pediatric and adult osteosarcoma reveals distinct molecular subsets with potentially targetable alterations. (2019)
  • Author(s): Suehara Y, Alex D, Bowman A, Middha S, Zehir A, Chakravarty D, Wang L, Jour G, Nafa K, Hayashi T, Jungbluth AA, Frosina D, Slotkin E, Shukla N, Meyers P, Healey J, Hameed M, Ladanyi M.
  • Journal Title: Clinical Cancer Research Volume: 25 Issue: 21 Pages: 6346-6356
  • DOI: 10.1158/1078-0432.ccr-18-4032
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Protein Expression Profiles Corresponding to Histological Changes with Denosumab Treatment in Giant Cell Tumors of Bone. (2019)
  • Author(s): Suehara Y, Okubo T, Kurihara T, Hayashi T, Kohsaka S, Kazuno S, Sano K, Hasegawa N, Miura Y, Akaike K, Kim Y, Takamochi K, Takahashi F, Ueno T, Kaneko K, Saito T.
  • Journal Title: Proteomics Clin Appl Volume: 13 Issue: 5 Pages: 1800147-1800147
  • DOI: 10.1002/prca.201800147
  • Peer Reviewed
• [Presentation] 臨床的治療実用可能な遺伝子変化の骨・軟部腫瘍における効率的同定法 (2022)
  • Author(s): 原 義之, 市田 美夕, 五木田 茶舞, 菊田 一貴, 鳥越 知明, 元井 紀子, 安田 政実, 平林 かおる, 林 大久生, 齋藤 剛, 石島 旨章
  • Organizer: 第37回日本整形外科学会基礎学術集会
• [Presentation] Whole exome sequence/RNA sequenceによる腱滑膜巨細胞腫の新規融合遺伝子、遺伝子変異の同定 (2022)
  • Author(s): 津田 祐輔, 平田 真, 小林 寛, 河野 博隆, 西田 佳弘, 大隈 知威, 阿江 啓介, 川井 章, 穴澤 卯圭, 末原 義之, 松田 浩一
  • Organizer: 第55回日本整形外科学会骨・軟部腫瘍学術集会
• [Presentation] Ewing肉腫における血漿中のcell-free RNAを用いた高感度融合遺伝子解析 (2022)
  • Author(s): 長谷川 延彦, 高阪 真路, 岩田 慎太郎, 川井 章, 齋藤 剛, 佐々 恵太, 窪田 大介, 末原 義之, 高木 辰哉, 間野 博行, 石島 旨章
  • Organizer: 第95回日本整形外科学会学術集会
• [Presentation] Comprehensive transcriptome analysis of Cap site at the 5'end in osteosarcomas. (2021)
  • Author(s): Sano K, Suehara Y, Oguchi A, Sasa K, Hayashi T, Kurihara T, AkaikeK, Kim Y, Okubo T, Kubota D, Takagi T, Murakawa Y, SaitoT.
  • Organizer: 2021 Annual Meeting of Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] Comprehensive transcriptome analysis of endoplasmic reticulum stress in osteosarcomas. (2020)
  • Author(s): Kurihara T, Suehara Y, Hayashi T, Sano K, Sasa K, Kubota D, Akaike K, Okubo T, Kim Y, Saito T.
  • Organizer: European Society For Medical Oncology, ASIA 2020
  • Int'l Joint Research
• [Presentation] Results of a randomized phase II/III study comparing perioperative adriamycin plus ifosfamide (AI) and gemcitabine plus docetaxel (GD) for operable high-grade soft tissue sarcomas (STS): Japan Clinical Oncology Group study JCOG1306. (2020)
  • Author(s): Tanaka K, Mizusawa J, Kawai A, Nakayama R, Tsukushi S, Asanuma K, Matsumoto Y, Hiraga H, Hiraoka K, Watanuki M, Yonemoto T, Abe S, Katagiri H, Nishida Y, Nagano A, Suehara Y, Fukuda H, Ozaki T, Iwamoto Y.
  • Organizer: American Society of Clinical Oncology, 2020 Annual Meeting
  • Int'l Joint Research
• [Presentation] Detection of Fusion Genes in Inflammatory Myofibroblastic Tumor/Inflammatory Pseudotumor by Nanostring-Based Screening System. (2020)
  • Author(s): Kurihara T, Suehara Y, Akaike K, Hayashi T, Kohsaka S, Hasegawa N, Takagi T, Okubo T, Kim Y, Yao T, Kaneko K, Saito T.
  • Organizer: USCAP, the 2020 Annual Meeting
  • Int'l Joint Research
• [Presentation] Detection of fusion genes in inflammatory myofibroblastic tumor by NanoString and immunohistochemistry. (2020)
  • Author(s): Kurihara T, Suehara Y, Sano K, Akaike K, Hayashi T, Hasegawa N, Takagi T, Okubo T, Yao T, Kaneko K, Saito T.
  • Organizer: 2020 Annual Meeting of Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] Expression Analysis of proteins associated with the regulation of c-kit and surgical material in gastrointestinal stromal tumor in which the effectiveness of HSP90 inhibitors has been pointed out. (2020)
  • Author(s): Sasa K, Suehara Y, Okubo T, Hayashi T, Sano K, Kurihara T, Akaike K, Ishii M, Kim Y, Kaneko K, Saito T.
  • Organizer: 2020 Annual Meeting of Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] Protein profiling of cell lines and surgical specimens reveal that heat shock protein 90-beta may be involved in the malignancy of synovial sarcoma. (2020)
  • Author(s): Sano K, Suehara Y, Sasa K, Kurihara T, Akaike K, Ishii M, Kim Y, Okubo T, Hayashi T, Kaneko K, Saito T.
  • Organizer: 2020 Annual Meeting of Orthopaedic Research Society
  • Int'l Joint Research
• [Presentation] シンポジウム:骨・軟部腫瘍研究の最前線 ゲノムシークエンシング 骨軟部腫瘍におけるクリニカルシークエンス (2020)
  • Author(s): 末原 義之、林 大久生、加藤 俊介、Marc Ladanyi、齋藤 剛
  • Organizer: 第35回日本整形外科学会基礎学術集会
  • Invited
• [Presentation] 優秀演題賞：高悪性骨肉腫の新規治療標的にいてのサンプリング部位による変化検討 (2020)
  • Author(s): 末原 義之, Alex Deepu, 林 大久生, Bowman Anita, Middha Sumit, Zehir Ahmet, Wang Lu, Meyers Paul, Healey John, Hameed Meera, Ladanyi Marc
  • Organizer: 第58回日本癌治療学会学術集会
  • Invited
• [Presentation] 骨肉腫と小胞体ストレス応答との関連性 (2020)
  • Author(s): 栗原 大聖, 末原 義之, 佐々 恵太, 佐野 圭, 赤池 慶祐, 窪田 大介, 林 大久生, 高木 辰哉, 金子 和夫, 齋藤 剛
  • Organizer: 第93日本整形外科学会学術総会
• [Presentation] 骨・軟部肉腫と小胞体ストレス応答との関連性 (2020)
  • Author(s): 栗原 大聖, 末原 義之, 赤池 慶祐, 林 大久生, 金 栄智, 大久保 武人, 高木 辰哉, 金子 和夫, 齋藤 剛
  • Organizer: 第53回日本整形外科学会 骨・軟部腫瘍学術集会
• [Presentation] Efficacy of IRE1α-XBP1 inhibitors in osteosarcomas (2019)
  • Author(s): Kurihara T, S Suehara Y, Hayashi T, Takagi T, Akaike K, Sano K, Kaneko K, Saito T.
  • Organizer: Connective Tissue Oncology Society, 24th Annual Meeting, November 13-16, 2019, Tokyo, Japan
  • Int'l Joint Research
• [Presentation] 骨肉腫に対するIRE1α阻害剤のin vivoとin vitroでの有効性. (2019)
  • Author(s): 栗原大聖, 末原義之, 赤池慶祐, 林大久生, 金 栄智, 大久保武人, 金子和夫, 齋藤 剛.
  • Organizer: 第92回日本整形外科学会学術総会, パシフィコ横浜, 横浜