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A study for molecular mechanisms that enable leaf acquisition in angiosperms and their functional analysis

Research Project

Project/Area Number 19K23742
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0702:Biology at cellular to organismal levels, and related fields
Research InstitutionThe University of Tokyo

Principal Investigator

Nakayama Hokuto  東京大学, 大学院理学系研究科(理学部), 助教 (30610935)

Project Period (FY) 2019-08-30 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
KeywordsAmborella trichopoda / Arabidopsis thaliana / Evo-Devo / RNA-seq / Solanum lycopersicum / インフォマティクス / 進化発生学 / 葉 / 形態 / 発生進化
Outline of Research at the Start

本研究は、現生の被子植物で最基部に位置するAmborella trichopoda(アンボレラ)を含む、ゲノム配列がすでに解読済みの複数のモデル植物の葉原基などを用いてRNA-seqを行ない、その結果を基部の陸上植物などと比較し、被子植物が葉を獲得を可能にした分子機構を世界に先駆けて明らかにしようとするものである。

Outline of Final Research Achievements

The molecular mechanisms that enable leaf acquisition in angiosperms were investigated. To investigate it, RNA -seq was performed using simple-leafed species, Amborella (Amborella trichopoda) and Arabidopsis (Arabidopsis thaliana). DEG analysis, Go enrichment analysis, and comparative Gene Co-expression network revealed that the gene expression profiles of leaves of the two species were similar, even though they are phylogenetically distant from each other. This suggested that the developmental program of simple leaves in angiosperms was already established in early diverged angiosperms such as Amborella.

Academic Significance and Societal Importance of the Research Achievements

本研究により、被子植物における単葉発生のメカニズムが、現存する被子植物の系統樹の中で最基部に位置し、単葉を形成するアンボレラ(Amborella trichopoda)とシロイヌナズナとの間で類似していることが初めて示唆された。これを端緒に、これからの解析により、これまで不明であった被子植物の葉の獲得とその進化についての理解が深まると考えられる。

Report

(4 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • Research Products

    (7 results)

All 2021 2020 Other

All Int'l Joint Research (2 results) Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (3 results)

  • [Int'l Joint Research] University of California, Davis(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] University of California, Davis(米国)

    • Related Report
      2020 Research-status Report
  • [Journal Article] Leaf form diversification in an ornamental heirloom tomato results from alterations in two different HOMEOBOX genes2021

    • Author(s)
      Nakayama Hokuto、Rowland Steven D.、Cheng Zizhang、Zumstein Kristina、Kang Julie、Kondo Yohei、Sinha Neelima R.
    • Journal Title

      Current Biology

      Volume: 31 Issue: 21 Pages: 4788-4799.e5

    • DOI

      10.1016/j.cub.2021.08.023

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Leaf shape is a predictor of fruit quality and cultivar performance in tomato2020

    • Author(s)
      Rowland Steven D.、Zumstein Kristina、Nakayama Hokuto、Cheng Zizhang、Flores Amber M.、Chitwood Daniel H.、Maloof Julin N.、Sinha Neelima R.
    • Journal Title

      New Phytologist

      Volume: 226 Issue: 3 Pages: 851-865

    • DOI

      10.1111/nph.16403

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 中山北斗、塚谷裕一2021

    • Author(s)
      現生被子植物の最基部で分岐した系統であるAmborella trichopodaを用いたトランスクリプ トーム解析
    • Organizer
      日本植物学会 第85回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 現生被子植物の系統樹上で最基部に位置するAmborella trichopodaを用いた葉の獲得に関わる進化発生学的研究2020

    • Author(s)
      中山北斗、塚谷裕一
    • Organizer
      日本植物生理学会
    • Related Report
      2020 Research-status Report
  • [Presentation] 現生被子植物の系統樹状で最基部に位置するAmborella trichopodaを用いた葉の獲得に関わる進化発生学的研究2020

    • Author(s)
      中山北斗、塚谷裕一
    • Organizer
      日本植物生理学会
    • Related Report
      2019 Research-status Report

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Published: 2019-09-03   Modified: 2023-01-30  

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