• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidating the ecology and function of novel microorganisms that dominate oil fields

Research Project

Project/Area Number 19KK0389
Research Category

Fund for the Promotion of Joint International Research (Fostering Joint International Research (A))

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Mochimaru Hanako  国立研究開発法人産業技術総合研究所, 地質調査総合センター, 主任研究員 (90462861)

Project Period (FY) 2020 – 2023
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥15,210,000 (Direct Cost: ¥11,700,000、Indirect Cost: ¥3,510,000)
Keywords嫌気微生物 / 油ガス田 / 海洋底泥 / 細菌 / 古細菌 / メタン生成 / ガス田 / 海洋微生物 / Atribacterota / 嫌気培養 / Atribacteria / 新規微生物 / 分離培養 / 海洋 / 嫌気 / 培養
Outline of Research at the Start

本研究では,世界各地の油ガス田や海洋に普遍的に生息することが遺伝子解析から示唆されるが,未だ同定株の存在しない新門候補'Atribacteria'(系統群名JS1およびOP9)の生態解明を目的としている。実施者が油田微生物の培養で発見した両系統群の培養方法を海洋の試料を用いて未培養の海洋系統群に対して実施する。海洋の未培養微生物の培養および海洋試料のゲノム解析に精通する研究室との共同研究により、海洋系統群を分離培養し、その生態を解明する。得られた結果を原油分解メタン生成技術に活用することにより、資源の効率的な増産を目指す。

Outline of Final Research Achievements

Uncultured Atribacterota are known to be the dominant bacterial species in oil fields, marine sediments, and methane hydrate-containing strata. Although this phylum encompasses a phylogenetically diverse group, only one species has been isolated. This study aimed to isolate and identify new microorganisms from various marine samples. We isolated and characterized a culture from a new genus in the phylum Atribacterota. This is only the second isolate obtained from this phylum, and both strains have a membrane surrounding the nucleoid. Additionally, uncultured Archaea of the phylum Bathyarchaeota were successfully cultured from mangrove mud that can directly or indirectly utilize lignin-related substances.

Academic Significance and Societal Importance of the Research Achievements

微生物により原油がメタンガスに分解されている油田、メタンハイドレート含有層など、商業的に重要なメタン生成環境において、Atribacterota門細菌やBathyarchaeota門古細菌等が優占していることが知られている。しかし、分離株がほとんどなく、これらの微生物の環境中における役割は未知であった。これらの微生物の生態を明らかにすることはガス田の成因解明、探鉱、微生物によるメタンガスの増産に資する基盤的知見となる。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (7 results)

All 2023 2022 2021 2020

All Int'l Joint Research (3 results) Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 1 results)

  • [Int'l Joint Research] University of Hawaii(米国)2023

    • Year and Date
      2023-07-13
    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] University of Hawaii(米国)2022

    • Year and Date
      2022-07-18
    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] University of Hawaii(米国)2021

    • Year and Date
      2021-01-16
    • Related Report
      2023 Annual Research Report
  • [Journal Article] An iron corrosion-assisted H2-supplying system: a culture method for methanogens and acetogens under low H2 pressures2020

    • Author(s)
      Souichiro Kato, Motoko Takashino, Kensuke Igarashi, Hanako Mochimaru, Daisuke Mayumi, Hideyuki Tamaki
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 1-9

    • DOI

      10.1038/s41598-020-76267-z

    • Related Report
      2020 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Isolation and characterization of the novel deep subsurface Atribacterota member possessing membrane-bounded nucleoid2022

    • Author(s)
      Hiroki Kawamoto, Miho Watanabe, Hanako Mochimaru, Nozomi Nakahara, Xiang-Ying Meng, Hideyoshi Yoshioka, Nobuhiko Nomura, Hideyuki Tamaki
    • Organizer
      18th International Symposium on Microbial Ecology
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] タンニン酸により集積されたガス田の新規古細菌2022

    • Author(s)
      持丸 華子,青柳 智, 堀 知行, 井上 一大, 佐々木 拓郎, 吉岡秀佳
    • Organizer
      第35回日本微生物生態学会
    • Related Report
      2022 Research-status Report
  • [Presentation] 核膜様構造を持つ新規 Atribacterota門地下細菌の培養化と機能解明2022

    • Author(s)
      川本 大輝,渡邊 美穂,持丸 華子,中原 望,孟 憲英,片山 泰樹, 吉岡 秀佳,野村 暢彦,玉木 秀幸
    • Organizer
      第35回日本微生物生態学会
    • Related Report
      2022 Research-status Report

URL: 

Published: 2020-02-06   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi