|Budget Amount *help
¥29,900,000 (Direct Cost: ¥23,000,000、Indirect Cost: ¥6,900,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2008: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Actin cytoskeleton dynamics are regulated by a variety of actin-binding proteins, which cooperatively act in the assembly/disassembly and reorganization of actin filaments in cells. In this study, we have assessed the concentration of actin monomer or cofilin in the cytoplasm of living cells by the fluorescence decay after photobleaching (FDAP) assay. We found that cofilin is involved in the generation of more than half of the actin monomers in the cytoplasm. We also found that cofilin exhibits fast turnover between cytoplasm and actin filaments. These results suggest that the degree of dynamics of actin or cofilin determines the capacity of cell motility.