Roles of novel intracellular ion channels

• Project/Area Number: 20249004
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Kyoto University
• Principal Investigator: TAKESHIMA Hiroshi Kyoto University, 薬学研究科, 教授 (70212024)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000); Fiscal Year 2010: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2008: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
• Keywords: TRICチャネル / ミツグミン23 / 小胞体 / 細胞内Ca^<2+>ストア / リアノジン受容体 / 細胞内カルシウムストア / IP3受容体 / カルミン / Ca2+放出 / ミツグミン53

Research Abstract

Our group recently identified the TRIC channel and mitsugumin 23 (MG23) as novel intracellular ion channels, and focused on their structure-function relationships and physiological roles in this study. We defined electrophysiological characteristics of TRIC channel subtypes in single channel measurements and their essential physiological functions in alveolar epithelial and skeletal muscle cells using knockout mice. Moreover, we also clarified the three-dimentional volume and cation-specific channel feature of MG23 in single-particle imaging and single channel monitoring, respectively.

Research Products

• [Journal Article] Imparied Orail-mediateded resting Ca^<2+> entry reduces the cytosolic [Ca^<2+>] and SR Ca^<2+> loading in quitescent junctophilinl knockout myotubes. (2010)
  • Author(s): Li, H., Ding, X., Lopez, J.R., Takeshima, H., Ma, J., Allen, P.D., Eltit, J.M.
  • Journal Title: J.Biol.Chem. 285 Pages: 39171-39179
  • Peer Reviewed
• [Journal Article] Ca^<2+> overload and sarcoplasmic reticulum instability in tric-a null skeletal muscle. (2010)
  • Author(s): Zhao, X., Yamazaki, D., Park, K-H., Komazaki, S., Tjondrokoesoemo, A., Nishi, M., Lin, P., Hirata, Y., Brotto, M., Takeshima, H., Ma, J.
  • Journal Title: J.Biol.Chem. 285 Pages: 37370-37376
  • Peer Reviewed
• [Journal Article] Charade of the SR K^+-channel : two ion-channels, TRIC-A and TRIC-B, masquerade as a single K^+-channel. (2010)
  • Author(s): Pitt, S.J., Park, K-H., Nishi, M., Urashima, T., Aoki, S., Yamazaki, D., Ma, J., Takeshima, H., Sitsapesan, R.
  • Journal Title: Biophys.J. 99 Pages: 417-426
  • Peer Reviewed
• [Journal Article] MG53 constitutes a primary determinant of cardiac ischemic preconditioning. (2010)
  • Author(s): Cao, C-M., Zhang, Y., Weisleder, N., Ferrante, C., Wang, X., Lv, F., Zhang, Y., Song, R., Hwang, M., Jin, L., Guo, J., Peng, W., Li, G., Nishi, M., Takeshima, H., Ma, J., Xiao, R-P.
  • Journal Title: Circulation 121 Pages: 2565-2574
  • Peer Reviewed
• [Journal Article] Facilitation of DNA damage- induced apoptosis by endoplasmic reticulum protein mitsugumin23. (2010)
  • Author(s): Yamazaki, T., Sasaki, N., Nishi, M., Takeshima, H.
  • Journal Title: Biochem.Biophys.Res. Commun. 392 Pages: 196-200
  • Peer Reviewed
• [Journal Article] Charade of the SR K^+-channel : two ion-channels, TRIC-A and TRIC-B, masquerade as a single K^+-channel. (2010)
  • Author(s): Pitt SJ, Park K-H, Nishi M, Urashima T, Aoki S, Yamazaki D, Ma J, Takeshima H., Sitsapesan R.
  • Journal Title: Biophys.J. Volume: 99 Pages: 417-426
  • Peer Reviewed
• [Journal Article] Ca^<2+> overload and sarcoplasmic reticulum instability in tric-a null skeletal muscle. (2010)
  • Author(s): Zhao X, Yamazaki D, Park K-H, Komazaki S, Tjondrokoesoemo A, Nishi M, Hirata Y, Brotto M, Takeshima H., Ma, J.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 37370-37376
  • Peer Reviewed
• [Journal Article] Mitsugumin 23 forms massive bowl-shaped assembly and cation-selective channel. (2010)
  • Author(s): Venturi E, Mio K, Nishi M, Ogura T, Moriya T, Okuda K, Kakizawa S, Sitapeasan R, Sato C., Takeshima H
  • Journal Title: Biochemistry Volume: 50(印刷中) Pages: 2623-2632
  • Peer Reviewed
• [Journal Article] Facilitation of DNA damage-induced apoptosis by endoplasmic reticulum protein mitsugumin23. (2010)
  • Author(s): Yamazaki, T., Sasaki, N., Nishi, M., Takeshima, H.
  • Journal Title: Biochem. Biophys. Res. Commun. 396 Pages: 196-200
  • Peer Reviewed
• [Journal Article] Molecular characterization of flubendiamide sensitivity in Iepidopterous ryanodine receptor. (2010)
  • Author(s): Kato, K., Kiyonaka, S., Sawaguchi, Y., Tohnish, M., Masaki, T., Yasokawa, N., Mizuno, Y., Mori, E., Inoue, K., Hamachi, I., Takeshima, H., Mori, Y.
  • Journal Title: Biochemistry 48 Pages: 10342-10352
  • Peer Reviewed
• [Journal Article] Essential role of TRIC-B channel in Ca^<2+>- handling of alveolar epithelium and perinatal lung maturation. (2009)
  • Author(s): Yamazaki, D., Komazaki, S., Nakanishi, H., Mishima, A., Nishi, M., Yazawa, M., Yamazaki, T., Taguchi, R., Takeshima, H.
  • Journal Title: Development 136 Pages: 2355-2361
  • Peer Reviewed
• [Journal Article] Mitsugumin 53-mediated maintenance of K^+ currents in cardiac myocytes. (2009)
  • Author(s): Masumiya, H., Asaumi, Y., Nishi, M., Minamisawa, S., Adachi-Akahane, S., Yoshida, M., Kangawa, K., Ito, K., Kagaya, Y., Yanagisawa, T., Yamazaki, T., Ma, J., Takeshima, H.
  • Journal Title: Channels 3 Pages: 6-11
  • Peer Reviewed
• [Journal Article] MG53 regulates membrane budding and exocytosis in muscle cells. (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Pan, Z., Nishi, M., Ko, J-K., Komazaki, S., Takeshima, H.^*, Ma, J.^* (*^co-corresponding authors)
  • Journal Title: J.Biol.Chem. 284 Pages: 3314-3322
  • Peer Reviewed
• [Journal Article] MG53 nucleates assembly of cell membrane repair machinery. (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Matsuda, N., Nishi, M., Hwang, M., Ko, J-K., Lin, P., Thornton, A., Zhao, X., Pan, Z., Komazaki, S., Brotto, M., Takeshima, H.^*, Ma, J.^* (*^co-corresponding authors)
  • Journal Title: Nature Cell Biol. 11 Pages: 56-64
  • Peer Reviewed
• [Journal Article] Essential role of TRIC-B channel in Ca2+ handling of alveolar epithelium and perinatal lung maturation. (2009)
  • Author(s): Yamazaki, D., Komazaki, S., Nakanishi, H., Mishima, A., Nishi, M., Yazawa, M., Yamazaki, T., Taguchi, R., Takeshima, H.
  • Journal Title: Development 136 Pages: 2355-2361
  • Peer Reviewed
• [Journal Article] MG53 nucleates assembly of cell membrane repair machinery. (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Matsuda, N., Nishi, M., Hwang, M., Ko, J-K., Lin, P., Thornton, A., Zhao, X., Pan, Z., Komazaki, S., Brotto, M., Takeshima, H., Ma, J
  • Journal Title: Nature Cell Biol. 11 Pages: 56-64
  • Peer Reviewed
• [Journal Article] TRPM2-mediated Ca^<2+> influx induces chemokine production in monocytes that aggravates inflammatory neutrophil infiltration. (2008)
  • Author(s): Yamamoto, S., Shimizu, S., Kiyonaka, S., Takahashi, N., Wajima, T., Hara, Y., Negoro, T., Hiroi, T., Kiuchi, Y., Okada, T., Kaneko, S., Lange, I., Fleig, A., Penner, R., Nishi, M., Takeshima, H, Mori, Y.
  • Journal Title: Nature Med. 14 Pages: 738-747
  • Peer Reviewed
• [Journal Article] Single-channel characterization of the rabbit recombinant RyR2 reveals a novel inactivation property of physiological concentrations of ATP. (2008)
  • Author(s): Stewart, R., Song, L., Carter, S.M., Sigalas, C., Zaccai, N.R., Kanamarlapudi, V., Bhat, M.B., Takeshima, H., Sitsapesan, R.
  • Journal Title: J.Membr.Biol. 222 Pages: 65-77
  • Peer Reviewed
• [Journal Article] Sarcalumenin alleviates stress-induced cardiac dysfunction by improving Ca^<2+> handling of the sarcoplasmic reticulum. (2008)
  • Author(s): Shimura, M., Minamisawa, S., Takeshima, H., Jiao, Q., Bai, Y., Umemura, S., Ishikawa, Y.
  • Journal Title: Cardiovasc. Res. 77 Pages: 362-370
  • Peer Reviewed
• [Journal Article] New molecular components supporting ryanodine receptor-mediated Ca2+ release : roles of junctophilin and TRIC (2008)
  • Author(s): Yamazaki, T., Yamazaki, D., Takeshima, H.
  • Journal Title: Pharmacol. Ther. 121 Pages: 265-272
  • Peer Reviewed
• [Journal Article] Single-channel characterization of the rabbit recombinant RyR2 reveals a novel inactivation property of physiological concentrations of ATP (2008)
  • Author(s): Stewart, R., Song, L., Carter, S. M., Sigalas, C., Zaccai, N. R., Kanamarlapudi, V., Bhat, M. B., Takeshima, H., Sitsapesan, R.
  • Journal Title: J. Membr. Biol. 222 Pages: 65-77
  • Peer Reviewed
• [Presentation] Immuno-proteomic approach to E-C coupling : junctophilin and TRIC channel in cardiac Ca^<2+> release (2010)
  • Author(s): Takeshima, H.
  • Organizer: IUPS satellite symposium
  • Place of Presentation: ホテルフジタ(京都)
  • Year and Date: 2010-07-28
• [Presentation] TRIC channel subtypes and SR/ER Ca^<2+> release. Gordon Research Conference. (2009)
  • Author(s): Takeshima, H.
  • Organizer: Muscle Excitation-Contraction Coupling
  • Place of Presentation: in Waterville Valley, USA.
  • Year and Date: 2009-06-18
• [Presentation] TRIC channel subtypes and SR/ER Ca2+ release. (2009)
  • Author(s): Hiroshi Takeshima
  • Organizer: Gordon Research Conference ; Muscle Excitation-Contraction Coupling.
  • Place of Presentation: Waterville Valley, USA.
  • Year and Date: 2009-06-18
• [Presentation] Molecular components supporting ryanodine receptor-mediated Ca^<2+> release : roles of junctophilin and TRIC channel in cardiac Ca^<2+> release." (2009)
  • Author(s): Takeshima, H.
  • Organizer: JST Japan-Mexico Scientific Workshop
  • Place of Presentation: in Universidad Nacional Autonoma de Mexico (UNAM), Mexico City
  • Year and Date: 2009-02-25
• [Book] トランスポートソームの世界 (2011)
  • Author(s): 金井好克、竹島浩、森泰生、久保義弘編集
  • Total Pages: 471
  • Publisher: 京都廣川書店
• [Book] トランスポートソームの世界 (2010)
  • Author(s): 金井好克、竹島浩、森泰生、久保義弘
  • Total Pages: 471
  • Publisher: 京都廣川書店
• [Remarks] ホームページ等
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/index.html