Analysis of the molecular basis for cold adaptation of psychrotrophic bacteria

• Project/Area Number: 20360372
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyoto University
• Principal Investigator: KURIHARA Tatsuo 京都大学, 化学研究所, 准教授 (70243087)
• Co-Investigator(Kenkyū-buntansha): ESAKI Nobuyoshi 京都大学, 化学研究所, 教授 (50135597) ; MIHARA Hisaaki 京都大学, 化学研究所, 助教 (30324693) ; KAWAMOTO Jun 京都大学, 化学研究所, 助教 (90511238)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2008: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
• Keywords: 微生物 / 特殊環境 / 好冷微生物 / 低温適応 / 生体膜 / タンパク質 / 脂質 / 生物・生体工学 / 高度不飽和脂肪酸 / エイコサペンタエン酸 / リン脂質

Research Abstract

Cold-adaptation mechanism of Shewanella livingstonensis Ac10 was analyzed. We identified cold-inducible proteins of this bacterium and analyzed their functions. We found that PspA involved in the response to membrane stress and MreB involved in cell division are important for cold adaptation. We also found that phospholipids containing eicosapentaenoyl group interact with various membrane proteins and affect their folding and properties to maintain the function of the cell membrane at low temperatures.

Research Products

• [Journal Article] Identification of cold-inducible inner membrane proteins of the psychrotrophic bacterium, Shewanella livingstonensis Ac10, by proteomic analysis (2012)
  • Author(s): Park J, Kawamoto J, Esaki N, Kurihara T
  • Journal Title: Extremophiles Volume: 16 Pages: 227-236
  • Peer Reviewed
• [Journal Article] Eicosapentaenoic acid plays a role in stabilizing dynamic membrane structure in the deep-sea piezophile Shewanella violacea : a study employing high-pressure time-resolved fluorescence anisotropy measurement (2012)
  • Author(s): Usui K, Hiraki T, Kawamoto J, Kurihara T, Nogi Y, Kato C, Abe F
  • Journal Title: Biochim Biophys Acta Volume: 1818 Pages: 574-583
  • Peer Reviewed
• [Journal Article] Identification of cold-inducible inner membrane proteins of the psychrotrophic bacterium, Shewanella livingstonensis Ac1O, by proteomic analysis (2012)
  • Author(s): Park, Jungha, et al
  • Journal Title: Extremophiles Volume: 16 Pages: 227-236
  • Peer Reviewed
• [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures (2011)
  • Author(s): Kawamoto J, Sato T, Nakasone K, Kato C, Mihara H, Esaki N, Kurihara T
  • Journal Title: Environ Microbiol Volume: 13 Pages: 2293-2298
  • Peer Reviewed
• [Journal Article] In vitro refolding of an OmpA homolog, amajor cold-inducible outer membrane protein, from a psychrotrophic bacterium, Shewanella livingstonensis Ac10, Dai X-Z (2011)
  • Author(s): Kawamoto J, Esaki N, Kurihara T
  • Journal Title: Journal of Japanese Society for Extremophiles Volume: 10 Pages: 90-96
  • Peer Reviewed
• [Journal Article] Differential roles of internal and terminal double bonds in docosahexaenoic acid : Comparative study of cytotoxicity of polyunsaturated fatty acids to HT-29 human colorectal tumor cell line (2011)
  • Author(s): Sato SB, Sato S, Kawamoto J, Kurihara T
  • Journal Title: Prostaglandins Leukot Essent Fatty Acids Volume: 84 Pages: 31-37
  • Peer Reviewed
• [Journal Article] Thermal stability of cytochrome c(5) of pressure-sensitive Shewanella livingstonensis (2011)
  • Author(s): Masanari M, Wakai S, Tamegai H, Kurihara T, Kato C, Sambongi Y
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 1859-1861
  • Peer Reviewed
• [Journal Article] Piezotolerance of the respiratory terminaloxidase activity of the piezophilic Shewanella violacea DSS12 as compared withnon-piezophilic Shewanella species (2011)
  • Author(s): Tamegai H, Ota Y, Haga M, Fujimori H, Kato C, Nogi Y, Kawamoto J, Kurihara T, Sambongi Y
  • Journal Title: Biosci Biotechnol Biochem Volume: 75 Pages: 919-924
  • Peer Reviewed
• [Journal Article] 好冷性細菌が生産する高度不飽和脂肪酸含有リン脂質の生理機能 (2011)
  • Author(s): 川本純, 栗原達夫
  • Journal Title: バイオサイエンスとインダストリー Volume: 69 Pages: 305-307
  • NAID: 10029237036
• [Journal Article] In vitro refolding of an OmpA homolog, a major cold-inducible outer membrane protein, from a psychrotrophic bacterium, Shewanella livingstonensis Ac10 (2011)
  • Author(s): Dai, Xian-Zhu, et al
  • Journal Title: Journal of Japanese Society for Extremophiles Volume: 10 Pages: 90-96
  • Peer Reviewed
• [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures (2011)
  • Author(s): Kawamoto, Jun, et al
  • Journal Title: Environ.Microbiol. Volume: 13 Pages: 2293-2298
  • Peer Reviewed
• [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in apiezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures (2011)
  • Author(s): Kawamoto, Jun, et al.
  • Journal Title: Environ.Microbiol.
  • Peer Reviewed
• [Journal Article] Differential roles of internal and terminal double bonds in docosahexaenoic acid : comparative study of cytotoxicity of polyunsaturated fatty acids to HT-29 human colorectal tumor cell line (2011)
  • Author(s): Sato, Satoshi B., et al.
  • Journal Title: Prostaglandins Leukot.Essent.Fatty Acids Volume: 84 Pages: 31-37
  • Peer Reviewed
• [Journal Article] 低温細菌における長鎖高度不飽和脂肪酸の生合成と機能 (2010)
  • Author(s): 栗原達夫, 川本純, 江崎信芳
  • Journal Title: 蛋白質核酸酵素 Volume: 55 Pages: 94-99
  • NAID: 40016985470
• [Journal Article] 低温細菌における長鎖高度不飽和脂肪酸の生合成と機能 (2010)
  • Author(s): 栗原,達夫, et al.
  • Journal Title: 蛋白質 核酸 酵素 Volume: 55 Pages: 94-99
  • NAID: 40016985470
• [Journal Article] Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium (2009)
  • Author(s): Kawamoto J, Kurihara T, Yamamoto K, Nagayasu M, Tani Y, Mihara H, Hosokawa M, Baba T, Sato SB, Esaki N
  • Journal Title: J Bacteriol Volume: 191 Pages: 632-640
  • Peer Reviewed
• [Journal Article] 低温細菌における高度不飽和脂肪酸含有リン脂質の機能 (2009)
  • Author(s): 栗原達夫
  • Journal Title: 生化学 Volume: 81 Pages: 716-719
  • NAID: 10026298336
• [Journal Article] 好冷性細菌の低温適応に関わるタンパク質とリン脂質 (2009)
  • Author(s): 栗原達夫, 川本純, 江崎信芳
  • Journal Title: 生化学 Volume: 81 Pages: 1072-1079
  • NAID: 10026299872
• [Journal Article] 低温下で機能する酵素 (2009)
  • Author(s): 江崎信芳
  • Journal Title: 化学工学 Volume: 73 Pages: 324-327
  • NAID: 10026200625
• [Journal Article] Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium, Shewanella livingstotiensis Ac10 (2009)
  • Author(s): Kawamoto, Jun, et al.
  • Journal Title: J. Bacteriol. 191 Pages: 632-640
  • Peer Reviewed
• [Journal Article] Cold adaptation of eicosapentaenoic acid-less mutant of Shewanella livingstonensis Ac10 involving uptake and remodeling of synthetic phospholipids containing various polyimsaturated fatty acids (2008)
  • Author(s): Sato, Sho, et al.
  • Journal Title: Extremophiles 12 Pages: 753-761
  • Peer Reviewed
• [Journal Article] Proteomic studies of psychrophilic microorganisms (2008)
  • Author(s): Kurihara, Tatsuo, et al.
  • Journal Title: Psychrophiles : from Biodiversity to Biotechnology Pages: 333-344
• [Presentation] 細菌細胞膜における高度不飽和脂肪酸含有リン脂質の機能:局在化と膜タンパク質生合成における機能 (2012)
  • Author(s): 栗原達夫
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都市
  • Year and Date: 2012-03-25
• [Presentation] 細菌細胞膜における高度不飽和脂肪酸含有リン脂質の機能:局在化と膜タンパク質生合成における機能 (2012)
  • Author(s): 栗原達夫, et al
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都市・京都女子大学(招待講演)
  • Year and Date: 2012-03-25
• [Presentation] 細菌細胞膜におけるリン脂質の局在化と生理機能 (2011)
  • Author(s): 栗原達夫
  • Organizer: 京都大学微生物科学寄附研究部門主催シンポジウム「微生物科学研究の現状と展望」
  • Place of Presentation: 京都市
  • Year and Date: 2011-06-23
• [Presentation] Construction of protein expression system by using cold-adapted bacteria as the host (2010)
  • Author(s): Kurihara T
  • Organizer: MicroPerm Workshop
  • Place of Presentation: Potsdam, Germany
  • Year and Date: 2010-11-08
• [Presentation] Phospholipids involved in cold adaptation of an Antarctic psychrotrophic bacterium, Shewanella livingstonensis Ac10 (2009)
  • Author(s): Kurihara T
  • Organizer: NAIST Global COE International Symposium 2009 Environmental Adaptation
  • Place of Presentation: 奈良県生駒市
  • Year and Date: 2009-11-13
• [Presentation] Cold-adaptation mechanism of an Antarctic psychrotrophic bacterium (2009)
  • Author(s): urihara T
  • Organizer: Shewanella livingstonensis Ac10, Xth SCAR International Biology Symposium
  • Place of Presentation: 札幌市
  • Year and Date: 2009-08-27
• [Presentation] Cold-adaptation mechanism of an Antarctic psychrotrophic bacterium, Shewanella livinzstonensis Ac10 (2009)
  • Author(s): Kurihara, Tatsuo, et al.
  • Organizer: Xth SCAR International Biology Symposium
  • Place of Presentation: 北海道札幌市
  • Year and Date: 2009-08-27
• [Presentation] 海洋性低温菌Shewanella livingstonensis Ac10における高度不飽和脂肪酸の機能 (2008)
  • Author(s): 栗原達夫
  • Organizer: マリンバイオテクノロジー学会
  • Place of Presentation: 京都市
  • Year and Date: 2008-05-25
• [Presentation] 海洋性低温菌Shewanella livingstonensis Ac10における高度不飽和脂肪酸の機能 (2008)
  • Author(s): 栗原, 達夫, et al
  • Organizer: マリンバイオテクノロジー学会
  • Place of Presentation: 京都府京都市
  • Year and Date: 2008-05-25
• [Presentation] Cold-adaptation mechanisms and applications of an Antarctic psychrotrophic bacteriu (2008)
  • Author(s): Kurihara T
  • Organizer: Shewanella livingstonensis Ac10, BIT Life Sciences 1st Annual World Congress of iBio2008
  • Place of Presentation: 中華人民共和国・杭州市
  • Year and Date: 2008-05-19
• [Book] 酵素利用技術大系好冷酵素の特性と利用法 (2010)
  • Author(s): 栗原達夫
  • Publisher: 株式会社エヌ・ティー・エス
• [Book] 酵素利用技術体系(好冷酵素の特性と利用法) (2010)
  • Author(s): 栗原,達夫
  • Publisher: エヌ・ティー・エス