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Whole-cell biocatalyst with non-natural membrane structure

Research Project

Project/Area Number 20360375
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionKobe University

Principal Investigator

KONDO Akihiko  Kobe University, 工学研究科, 教授 (40205547)

Co-Investigator(Kenkyū-buntansha) TANAKA Tsutomu  神戸大学, 自然科学系・先端融合研究環重点研究部, 助教 (90436551)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2010: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2009: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2008: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywordsタンパク質 / 酵素 / 非天然分子 / 酵母 / 細胞表層提示 / 菌体触媒 / バイオマス / 有機溶媒耐性
Research Abstract

Introduction of non-natural, functional molecules on the cell surface was developed for establishment of several stress, such as organic medium containing broth. Using biotin-streptavidin interaction, non-natural molecules were successfully incorporated onto yeast cell surface. In addition, novel enzymes, which can conjugate some functional molecules on the cell surfaces, were explored. The evaluation of cell surface modified microorganisms' functions are undergoing such as stress tolerance.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (10 results)

All 2011 2010 2009 2008

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (8 results)

  • [Journal Article] Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction.2010

    • Author(s)
      Tanaka, T., Masunari, S., Ishii, J., Wakamura, K., Segawa, M., Fukuda, H., Kondo, A.
    • Journal Title

      Journal of Biotechnology 145

      Pages: 79-83

    • Related Report
      2010 Final Research Report
  • [Journal Article] Displaying non-natural, functional molecules on yeast surfaces via biotin-streptavidin interaction.2010

    • Author(s)
      Tanaka, T., Masunari, S., Ishii, J., Wakamura, K., Segawa, M., Fukuda, H., Kondo, A.
    • Journal Title

      Journal of Biotechnology

      Volume: 145 Pages: 79-83

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 長期エタノール発酵に向けたアミラーゼ表層提示発現酵母株の創製2011

    • Author(s)
      山川瞬一・山田亮祐・田中勉・荻野千秋・近藤昭彦
    • Organizer
      化学工学会第76年会
    • Place of Presentation
      東京農工大学
    • Related Report
      2010 Final Research Report
  • [Presentation] 長期エタノール発酵に向けたアミラーゼ表層提示発現酵母株の創製2011

    • Author(s)
      山川瞬一・山田亮祐・田中勉・荻野千秋・近藤昭彦
    • Organizer
      化学工学会 第76年会
    • Place of Presentation
      東京農工大学(東京都)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Sortaseを用いたstreptavidinの新規修飾技術の開発2010

    • Author(s)
      松本拓也・田中勉・近藤昭彦
    • Organizer
      生物工学会62回大会
    • Place of Presentation
      宮崎シーガイアワールドコンベンションセンターサミット
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] セルラーゼ発現最適化バランス酵母を用いたセルロースからのエタノール発酵2010

    • Author(s)
      山田亮祐・田中勉・荻野千秋・近藤昭彦
    • Organizer
      生物工学会62回大会
    • Place of Presentation
      宮崎シーガイアワールドコンベンションセンターサミット
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Enzymatic protein conjugation in vivo2009

    • Author(s)
      Kanako Wakamura, Tsutomu Tanaka, Akihiko Kondo
    • Organizer
      APBioChEC 2009
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Enzymatic protein immobilization on particles2009

    • Author(s)
      Maiko Segawa, Kazusa Matsui, Tsutomu Tanaka, Chiaki Ogino, Akihiko Kondo
    • Organizer
      APBioChEC 2009
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Enzyme-mediated Site-specific protein modification2009

    • Author(s)
      Tsutomu Tanaka, Takayuki Sakamoto, Kanako Wakamura, Ryosuke Takase, Takuya Matsumoto, Shiori Sawamoto, Akihiko Kondo
    • Organizer
      APBioChEC 2009
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 新規酵素を用いたタンパク質への小分子修飾技術2008

    • Author(s)
      高瀬了奨, 田中勉, 福田秀樹, 近藤昭彦
    • Organizer
      生物工学会
    • Place of Presentation
      東北学院大学
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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